pCMV-eBE-S3

LOCUS       pCMV-eBE-S3               9499 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Expresses eBE-S3 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    pCMV-eBE-S3
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9499)
  AUTHORS   Wang L, Xue W, Yan L, Li X, Wei J, Chen M, Wu J, Yang B, Yang L, 
            Chen J
  TITLE     Enhanced base editing by co-expression of free uracil DNA 
            glycosylase inhibitor.
  JOURNAL   Cell Res. 2017 Aug 29. doi: 10.1038/cr.2017.111.
  PUBMED    28849781
REFERENCE   2  (bases 1 to 9499)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..9499
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        132..335
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     286..306
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     377..396
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        377..395
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             409..1095
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWG
                     GRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFL
                     SRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSN
                     EAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHI
                     LWATGLK"
     CDS             1144..5244
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5257..5505
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5518..5538
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5545..5598
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5599..5847
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5848..5868
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5869..5925
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5926..6174
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             6175..6195
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6196..6249
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6250..6498
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             6511..6531
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(6554..6571)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6560..6784
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6855..6871)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6855..6871)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6868..6890)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6879..6895
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6903..6933)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6948..6969
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7086..7103)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7257..7845)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7337..7356)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8016..8876)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8639..8658
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8877..8981)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9060..9079)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        9251..131
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
       61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gagctcagag
      421 actggcccag tggctgtgga ccccacattg agacggcgga tcgagcccca tgagtttgag
      481 gtattcttcg atccgagaga gctccgcaag gagacctgcc tgctttacga aattaattgg
      541 gggggccggc actccatttg gcgacataca tcacagaaca ctaacaagca cgtcgaagtc
      601 aacttcatcg agaagttcac gacagaaaga tatttctgtc cgaacacaag gtgcagcatt
      661 acctggtttc tcagctggag cccatgcggc gaatgtagta gggccatcac tgaattcctg
      721 tcaaggtatc cccacgtcac tctgtttatt tacatcgcaa ggctgtacca ccacgctgac
      781 ccccgcaatc gacaaggcct gcgggatttg atctcttcag gtgtgactat ccaaattatg
      841 actgagcagg agtcaggata ctgctggaga aactttgtga attatagccc gagtaatgaa
      901 gcccactggc ctaggtatcc ccatctgtgg gtacgactgt acgttcttga actgtactgc
      961 atcatactgg gcctgcctcc ttgtctcaac attctgagaa ggaagcagcc acagctgaca
     1021 ttctttacca tcgctcttca gtcttgtcat taccagcgac tgcccccaca cattctctgg
     1081 gccaccgggt tgaaaagcgg cagcgagact cccgggacct cagagtccgc cacacccgaa
     1141 agtgataaaa agtattctat tggtttagcc atcggcacta attccgttgg atgggctgtc
     1201 ataaccgatg aatacaaagt accttcaaag aaatttaagg tgttggggaa cacagaccgt
     1261 cattcgatta aaaagaatct tatcggtgcc ctcctattcg atagtggcga aacggcagag
     1321 gcgactcgcc tgaaacgaac cgctcggaga aggtatacac gtcgcaagaa ccgaatatgt
     1381 tacttacaag aaatttttag caatgagatg gccaaagttg acgattcttt ctttcaccgt
     1441 ttggaagagt ccttccttgt cgaagaggac aagaaacatg aacggcaccc catctttgga
     1501 aacatagtag atgaggtggc atatcatgaa aagtacccaa cgatttatca cctcagaaaa
     1561 aagctagttg actcaactga taaagcggac ctgaggttaa tctacttggc tcttgcccat
     1621 atgataaagt tccgtgggca ctttctcatt gagggtgatc taaatccgga caactcggat
     1681 gtcgacaaac tgttcatcca gttagtacaa acctataatc agttgtttga agagaaccct
     1741 ataaatgcaa gtggcgtgga tgcgaaggct attcttagcg cccgcctctc taaatcccga
     1801 cggctagaaa acctgatcgc acaattaccc ggagagaaga aaaatgggtt gttcggtaac
     1861 cttatagcgc tctcactagg cctgacacca aattttaagt cgaacttcga cttagctgaa
     1921 gatgccaaat tgcagcttag taaggacacg tacgatgacg atctcgacaa tctactggca
     1981 caaattggag atcagtatgc ggacttattt ttggctgcca aaaaccttag cgatgcaatc
     2041 ctcctatctg acatactgag agttaatact gagattacca aggcgccgtt atccgcttca
     2101 atgatcaaaa ggtacgatga acatcaccaa gacttgacac ttctcaaggc cctagtccgt
     2161 cagcaactgc ctgagaaata taaggaaata ttctttgatc agtcgaaaaa cgggtacgca
     2221 ggttatattg acggcggagc gagtcaagag gaattctaca agtttatcaa acccatatta
     2281 gagaagatgg atgggacgga agagttgctt gtaaaactca atcgcgaaga tctactgcga
     2341 aagcagcgga ctttcgacaa cggtagcatt ccacatcaaa tccacttagg cgaattgcat
     2401 gctatactta gaaggcagga ggatttttat ccgttcctca aagacaatcg tgaaaagatt
     2461 gagaaaatcc taacctttcg cataccttac tatgtgggac ccctggcccg agggaactct
     2521 cggttcgcat ggatgacaag aaagtccgaa gaaacgatta ctccatggaa ttttgaggaa
     2581 gttgtcgata aaggtgcgtc agctcaatcg ttcatcgaga ggatgaccaa ctttgacaag
     2641 aatttaccga acgaaaaagt attgcctaag cacagtttac tttacgagta tttcacagtg
     2701 tacaatgaac tcacgaaagt taagtatgtc actgagggca tgcgtaaacc cgcctttcta
     2761 agcggagaac agaagaaagc aatagtagat ctgttattca agaccaaccg caaagtgaca
     2821 gttaagcaat tgaaagagga ctactttaag aaaattgaat gcttcgattc tgtcgagatc
     2881 tccggggtag aagatcgatt taatgcgtca cttggtacgt atcatgacct cctaaagata
     2941 attaaagata aggacttcct ggataacgaa gagaatgaag atatcttaga agatatagtg
     3001 ttgactctta ccctctttga agatcgggaa atgattgagg aaagactaaa aacatacgct
     3061 cacctgttcg acgataaggt tatgaaacag ttaaagaggc gtcgctatac gggctgggga
     3121 cgattgtcgc ggaaacttat caacgggata agagacaagc aaagtggtaa aactattctc
     3181 gattttctaa agagcgacgg cttcgccaat aggaacttta tgcagctgat ccatgatgac
     3241 tctttaacct tcaaagagga tatacaaaag gcacaggttt ccggacaagg ggactcattg
     3301 cacgaacata ttgcgaatct tgctggttcg ccagccatca aaaagggcat actccagaca
     3361 gtcaaagtag tggatgagct agttaaggtc atgggacgtc acaaaccgga aaacattgta
     3421 atcgagatgg cacgcgaaaa tcaaacgact cagaaggggc aaaaaaacag tcgagagcgg
     3481 atgaagagaa tagaagaggg tattaaagaa ctgggcagcc agatcttaaa ggagcatcct
     3541 gtggaaaata cccaattgca gaacgagaaa ctttacctct attacctaca aaatggaagg
     3601 gacatgtatg ttgatcagga actggacata aaccgtttat ctgattacga cgtcgatcac
     3661 attgtacccc aatccttttt gaaggacgat tcaatcgaca ataaagtgct tacacgctcg
     3721 gataagaacc gagggaaaag tgacaatgtt ccaagcgagg aagtcgtaaa gaaaatgaag
     3781 aactattggc ggcagctcct aaatgcgaaa ctgataacgc aaagaaagtt cgataactta
     3841 actaaagctg agaggggtgg cttgtctgaa cttgacaagg ccggatttat taaacgtcag
     3901 ctcgtggaaa cccgccaaat cacaaagcat gttgcacaga tactagattc ccgaatgaat
     3961 acgaaatacg acgagaacga taagctgatt cgggaagtca aagtaatcac tttaaagtca
     4021 aaattggtgt cggacttcag aaaggatttt caattctata aagttaggga gataaataac
     4081 taccaccatg cgcacgacgc ttatcttaat gccgtcgtag ggaccgcact cattaagaaa
     4141 tacccgaagc tagaaagtga gtttgtgtat ggtgattaca aagtttatga cgtccgtaag
     4201 atgatcgcga aaagcgaaca ggagataggc aaggctacag ccaaatactt cttttattct
     4261 aacattatga atttctttaa gacggaaatc actctggcaa acggagagat acgcaaacga
     4321 cctttaattg aaaccaatgg ggagacaggt gaaatcgtat gggataaggg ccgggacttc
     4381 gcgacggtga gaaaagtttt gtccatgccc caagtcaaca tagtaaagaa aactgaggtg
     4441 cagaccggag ggttttcaaa ggaatcgatt cttccaaaaa ggaatagtga taagctcatc
     4501 gctcgtaaaa aggactggga cccgaaaaag tacggtggct tcgatagccc tacagttgcc
     4561 tattctgtcc tagtagtggc aaaagttgag aagggaaaat ccaagaaact gaagtcagtc
     4621 aaagaattat tggggataac gattatggag cgctcgtctt ttgaaaagaa ccccatcgac
     4681 ttccttgagg cgaaaggtta caaggaagta aaaaaggatc tcataattaa actaccaaag
     4741 tatagtctgt ttgagttaga aaatggccga aaacggatgt tggctagcgc cggagagctt
     4801 caaaagggga acgaactcgc actaccgtct aaatacgtga atttcctgta tttagcgtcc
     4861 cattacgaga agttgaaagg ttcacctgaa gataacgaac agaagcaact ttttgttgag
     4921 cagcacaaac attatctcga cgaaatcata gagcaaattt cggaattcag taagagagtc
     4981 atcctagctg atgccaatct ggacaaagta ttaagcgcat acaacaagca cagggataaa
     5041 cccatacgtg agcaggcgga aaatattatc catttgttta ctcttaccaa cctcggcgct
     5101 ccagccgcat tcaagtattt tgacacaacg atagatcgca aacgatacac ttctaccaag
     5161 gaggtgctag acgcgacact gattcaccaa tccatcacgg gattatatga aactcggata
     5221 gatttgtcac agcttggggg tgactctggt ggttctacta atctgtcaga tattattgaa
     5281 aaggagaccg gtaagcaact ggttatccag gaatccatcc tcatgctccc agaggaggtg
     5341 gaagaagtca ttgggaacaa gccggaaagc gatatactcg tgcacaccgc ctacgacgag
     5401 agcaccgacg agaatgtcat gcttctgact agcgacgccc ctgaatacaa gccttgggct
     5461 ctggtcatac aggatagcaa cggtgagaac aagattaaga tgctctctgg tggttctccc
     5521 aagaagaaga ggaaagtcgg gcccgagggc agaggaagtc tgctaacatg cggtgacgtc
     5581 gaggagaatc ctggcccaac taatctgtca gatattattg aaaaggagac cggtaagcaa
     5641 ctggttatcc aggaatccat cctcatgctc ccagaggagg tggaagaagt cattgggaac
     5701 aagccggaaa gcgatatact cgtgcacacc gcctacgacg agagcaccga cgagaatgtc
     5761 atgcttctga ctagcgacgc ccctgaatac aagccttggg ctctggtcat acaggatagc
     5821 aacggtgaga acaagattaa gatgctcccc aagaagaaga ggaaagtcgc tactaacttc
     5881 agcctgctga agcaggctgg agacgtggag gagaaccctg gacctaccaa cctgtccgat
     5941 atcattgaga aagagaccgg caaacagctg gtgatccagg agagcatcct gatgctgccc
     6001 gaagaggtgg aggaagtgat cggcaacaag cccgagtccg acatcctggt gcacacagcc
     6061 tatgatgaat ccaccgacga gaacgtgatg ctgctgacct ccgatgctcc cgagtataaa
     6121 ccctgggcac tggtgatcca ggactctaat ggagagaaca agatcaagat gctgcccaag
     6181 aagaagagga aagtcgaagg acggggctcc ctcctgacct gtggcgatgt ggaagagaac
     6241 cccggcccca caaacctcag tgacattatc gagaaggaaa caggaaaaca gctcgtcatt
     6301 caagaatcta ttcttatgtt gcctgaggaa gtcgaagagg ttattggcaa taaacctgaa
     6361 tctgatattc ttgtccatac cgcttacgat gagtccacag atgaaaatgt tatgctgctc
     6421 acatctgacg caccagagta caaaccatgg gcgctcgtta ttcaagattc caacggcgaa
     6481 aacaaaatca aaatgctttc tggtggttct cctaaaaaaa aaagaaaggt gtgagtttaa
     6541 acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     6601 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     6661 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     6721 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
     6781 atggcttctg aggcggaaag aaccagctgg ggctcgatac cgtcgacctc tagctagagc
     6841 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     6901 cacaacatac gagccggaag cataaagtgt aaagcctagg gtgcctaatg agtgagctaa
     6961 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     7021 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     7081 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     7141 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     7201 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     7261 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     7321 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     7381 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     7441 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     7501 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     7561 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     7621 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     7681 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     7741 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     7801 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     7861 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     7921 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     7981 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     8041 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     8101 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     8161 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     8221 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     8281 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     8341 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     8401 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     8461 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     8521 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     8581 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     8641 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     8701 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     8761 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     8821 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     8881 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     8941 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     9001 ccacctgacg tcgacggatc gggagatcga tctcccgatc ccctagggtc gactctcagt
     9061 acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag
     9121 gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat
     9181 tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga
     9241 tatacgcgtt gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
     9301 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
     9361 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
     9421 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
     9481 gcagtacatc aagtgtatc
//