pAAV-CAG-SNIFP

LOCUS       pAAV-CAG-SNIFP       5686 bp DNA     circular SYN 21-APR-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5686)
  AUTHORS   Triple Threat
  TITLE     Direct Submission
  JOURNAL   Exported Jun 25, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..5686
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        279..556
                     /label=chicken beta-actin promoter
     regulatory      977..982
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             983..1942
                     /codon_start=1
                     /label=SNIFP
                     /translation="MSRDPLPFFPPLYLGGPEITTENCEREPIHIPGSIQPHGALLTAD
                     GHSGEVLQMSLNAATFLGQEPTVLRGQTLAALLPEQWPALQAALPPGCPDALQYRATLD
                     WPAAGHLSLTVHRVGELLILEFEPTEAWDSTGPHALRNAMSALESAPNLRALAEVATQT
                     VRELTGFDRVMLYKFAPDATGEVIAEARREGLHAFLGHRFPASLIPAQARALYTRHLLR
                     LTADTRAAAVPLDPVLNPQTNAPTPLGGAVLRATSPMHMQFLRNMGVRSSLSVSVVVGG
                     QLWGLIACHHQTPYVLPPDLRTTLEYLGRELSEQVQVKE"
     primer_bind     complement(1919..1971)
                     /label=0503-SNIFP-R
     misc_feature    1970..2558
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(2441..2452)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2601..2722
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3250..3838)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4009..4869)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4870..4974)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5000..5455)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 tctacgtagc catgctctag gaagagtacc attgacgtca ataatgacgt atgttcccat
       61 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      121 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
      181 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
      241 gcagtacatc tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg
      301 cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta
      361 attattttgt gcagcgatgg gggcgggggg gggggggggg cgcgcgccag gcggggcggg
      421 gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg
      481 cgcgctccga aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg
      541 aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc
      601 cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg
      661 ggacggccct tctcctccgg gctgtaatta gcgcttggtt taatgacggc ttgtttcttt
      721 tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg gggggagcgg
      781 ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg
      841 gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct
      901 ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag
      961 aattggatcc ggtaccgcca ccatgtcccg tgaccctctc cccttcttcc ctcccctgta
     1021 cttgggtggc cccgagatta cgactgagaa ctgcgagagg gaacccatcc acattccggg
     1081 aagcatacag cctcatgggg cactcctgac tgccgatgga cactctggcg aagtcctgca
     1141 gatgagcctg aacgcagcta cctttcttgg ccaggagcca actgtgcttc gagggcagac
     1201 actggcggcc ctcctgcctg agcagtggcc tgccttgcaa gcggccctcc ctcccggatg
     1261 tcccgatgcc ctccagtatc gggcgacact ggactggcca gccgcagggc atctcagtct
     1321 gactgtccat cgggttggtg agctcttgat cctggagttt gagcccacgg aagcatggga
     1381 ctctaccggt ccccatgccc ttagaaacgc catgtcagct ctcgagtctg caccaaacct
     1441 gagagctctg gccgaggtcg caacccagac cgtgcgggag ctgacgggct tcgatcgtgt
     1501 aatgctgtac aagttcgcac ccgatgctac aggcgaggtg atcgctgaag cgcgcagaga
     1561 aggactgcac gcgtttctgg ggcataggtt tccggcttct ctgattcccg cacaagctag
     1621 ggccctctat actcgccatc tgctgcggct taccgctgac acaagagccg cagctgtgcc
     1681 tctggaccca gtcctgaatc cccagaccaa tgccccaacg cccctcggag gggctgtgct
     1741 gcgcgccact agccccatgc acatgcagtt cctccggaat atgggcgtga ggtcctcact
     1801 gagcgtaagt gtggtggtag gagggcaact gtggggcctg attgcctgtc accaccagac
     1861 accttacgtg cttcctcctg atctgaggac aactctcgag tatctggggc gtgaactgtc
     1921 cgaacaggtg caagtcaaag aataagaatt cgatatcaag cttatcgata atcaacctct
     1981 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     2041 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     2101 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     2161 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     2221 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     2281 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     2341 caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc
     2401 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     2461 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     2521 tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgtc gacccgggcg
     2581 gccgcttcga gcagacatga taagatacat tgatgagttt ggacaaacca caactagaat
     2641 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
     2701 tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca
     2761 gggggagatg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtaaaatcga
     2821 taaggatctt cctagagcat ggctacgtag ataagtagca tggcgggtta atcattaact
     2881 acaaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg
     2941 aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg
     3001 agcgagcgcg cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     3061 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     3121 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     3181 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     3241 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     3301 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     3361 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     3421 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     3481 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     3541 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     3601 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     3661 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     3721 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     3781 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     3841 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     3901 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     3961 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     4021 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     4081 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     4141 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     4201 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     4261 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     4321 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     4381 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     4441 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     4501 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     4561 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     4621 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     4681 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     4741 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     4801 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     4861 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     4921 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     4981 tccccgaaaa gtgccaccta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat
     5041 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa
     5101 tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta
     5161 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca
     5221 ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat
     5281 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg
     5341 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc
     5401 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat
     5461 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
     5521 cgccagctgc gcgctcgctc gctcactgag gccgcccggg caaagcccgg gcgtcgggcg
     5581 acctttggtc gcccggcctc agtgagcgag cgagcgcgca gagagggagt ggccaactcc
     5641 atcactaggg gttccttgta gttaatgatt aacccgccat gctact
//