| 质粒名称: | p18sheLL |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 37321 |
| 存储实验室: | John Schiller |
|
载体骨架基本信息 |
|
| 载体名称: | custom |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | HPV18L1+L2,p18LLw |
| 物种来源: | H. sapiens (human) |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | CMV |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | p18sheLL was a gift from John Schiller (Addgene plasmid # 37321 ; http://n2t.net/addgene:37321 ; RRID:Addgene_37321) |
| 参考文献部分: | Genital transmission of HPV in a mouse model is potentiated by nonoxynol-9 and inhibited by carrageenan. Roberts JN, Buck CB, Thompson CD, Kines R, Bernardo M, Choyke PL, Lowy DR, Schiller JT. Nat Med. 2007 Jul;13(7):857-61. Epub 2007 Jul 1. 10.1038/nm1598 PubMed 17603495 |
买家导航
p18sheLL载体质粒基本信息
p18sheLL质粒图谱载体图谱和p18sheLL载体序列质粒序列多克隆位点信息
p18sheLL质粒载体简介
p18sheLL质粒序列载体序列
LOCUS p18sheLL 10723 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS p18sheLL
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10723)
AUTHORS Roberts JN, Buck CB, Thompson CD, Kines R, Bernardo M, Choyke PL,
Lowy DR, Schiller JT
TITLE Genital transmission of HPV in a mouse model is potentiated by
nonoxynol-9 and inhibited by carrageenan.
JOURNAL Nat Med. 2007 Jul;13(7):857-61. Epub 2007 Jul 1.
PUBMED 17603495
REFERENCE 2 (bases 1 to 10723)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..10723
/organism="synthetic DNA construct"
/mol_type="other DNA"
regulatory 7..16
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
misc_feature 3659..4247
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(3712..3732)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(4130..4141)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind complement(4284..4301)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 4290..4514
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 4560..4988
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(4647..4666)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 4857..4878
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(4997..5017)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
CDS 6946..7314
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/label=BleoR
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="KLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDDVT
LFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGREF
ALRDPAGNCVHFVAEEQD"
polyA_signal 7444..7565
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(7481..7500)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 7535..7554
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(7614..7630)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(7614..7630)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(7627..7649)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 7638..7654
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(7662..7692)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 7707..7728
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(7845..7862)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(8016..8601)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(8096..8115)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(8772..9632)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 9395..9414
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(9633..9737)
/gene="bla"
/label=AmpR promoter
primer_bind complement(9812..9831)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 10003..10382
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 10383..10586
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 10537..10557
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
protein_bind 10588..10606
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 10609..10627
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
primer_bind 10637..10661
/label=LNCX
/note="Human CMV promoter, forward primer"
ORIGIN
1 ccggtcgcca ccatggccct ctggagacca tccgataaca cagtgtactt gcccccaccc
61 agcgtcgccc gggtggtgaa cacagacgac tacgtcacca gaacctcaat cttctaccac
121 gccgggtcca gccggctgct gaccgtgggc aacccctact tccgcgtgcc cgccggcggc
181 ggaaacaaac aagacatccc caaagtcagc gcctatcagt accgggtgtt ccgcgtccaa
241 ctgcccgatc ccaacaagtt cggcctgccc gacacctcca tctacaaccc cgagacccag
301 aggctggtct gggcttgcgc cggcgtcgag atcgggaggg gccaacccct gggcgtgggg
361 ttgtccggcc accccttcta caacaagctg gacgataccg agtccagcca cgcagcaacc
421 agcaacgtct ccgaagatgt gcgcgataac gtcagcgtgg actacaaaca aacccaactg
481 tgcatcctgg gatgcgcacc cgccatcggc gagcattggg ccaaggggac cgcctgcaag
541 agcaggcccc tgagccaagg ggactgtcca cccctggagt tgaagaatac cgtgctcgag
601 gacggcgaca tggtggacac cggctacggc gctatggatt tctccaccct ccaggacacc
661 aagtgcgaag tgcccctcga catctgccaa agcatctgca agtaccccga ctacctccag
721 atgagcgccg acccctacgg cgacagcatg ttcttctgtc tcagaaggga acaattgttc
781 gcccgccact tctggaaccg ggccggcaca atgggagata cagtccccca gagcctgtac
841 atcaagggga ccggaatgag ggccagcccc gggtcctgcg tctacagccc aagcccctcc
901 gggagcatcg tcacaagcga tagccaactc ttcaacaagc cctactggct ccacaaagcc
961 caaggccaca ataacggggt gtgttggcac aaccagctgt tcgtgaccgt cgtggacaca
1021 accaggtcca caaacctgac catctgcgcc agcacccaaa gccccgtgcc cggccagtac
1081 gacgccacaa agttcaaaca atactctcgg cacgtggaag agtacgacct ccaattcatc
1141 ttccaactct gcaccatcac cctcaccgcc gacgtgatga gctacatcca ctccatgaac
1201 tcctccatcc tggaagactg gaatttcggc gtgccaccac cccctaccac ctccctcgtc
1261 gacacctaca gattcgtgca gagcgtggcc atcacatgcc agaaagacgc cgcccccgcc
1321 gagaacaaag acccatacga caaactgaaa ttctggaacg tcgacctgaa agagaaattc
1381 agcctggatc tggaccagta cccattgggc aggaagttcc tcgtgcaagc cggcctcagg
1441 agaaaaccaa caatcgggcc caggaagagg agcgccccca gcgcaaccac cagcagcaag
1501 cccgcaaaaa gggtcagagt gagggcacgc aaatgagcta gaactagtgg atcccccggg
1561 ctgcaggaat tcgatgggtc gacctgcaga agcttaaaac agctctgggg ttgtacccac
1621 cccagaggcc cacgtggcgg ctagtactcc ggtattgcgg tacccttgta cgcctgtttt
1681 atactccctt cccgtaactt agacgcacaa aaccaagttc aatagaaggg ggtacaaacc
1741 agtaccacca cgaacaagca cttctgtttc cccggtgatg tcgtatagac tgcttgcgtg
1801 gttgaaagcg acggatccgt tatccgctta tgtacttcga gaagcccagt accacctcgg
1861 aatcttcgat gcgttgcgct cagcactcaa ccccagagtg tagcttaggc tgatgagtct
1921 ggacatccct caccggtgac ggtggtccag gctgcgttgg cggcctacct atggctaacg
1981 ccatgggacg ctagttgtga acaaggtgtg aagagcctat tgagctacat aagaatcctc
2041 cggcccctga atgcggctaa tcccaacctc ggagcaggtg gtcacaaacc agtgattggc
2101 ctgtcgtaac gcgcaagtcc gtggcggaac cgactacttt gggtgtccgt gtttcctttt
2161 attttattgt ggctgcttat ggtgacaatc acagattgtt atcataaagc gaattggatt
2221 gcggccgcat ggtcagccat agggctgcca ggaggaagag agccagcgtg accgatctgt
2281 acaagacctg caagcagagc ggcacctgcc cccccgacgt ggtgcccaaa gtcgaaggga
2341 caaccctggc cgacaagatc ctccagtgga gctctttggg catcttcctc ggcggcttgg
2401 ggatcggcac cgggtccggc accggcggca ggaccggcta tatccccctc gggggcagga
2461 gcaacaccgt cgtcgacgtg ggccccacca ggcctcccgt cgtgatcgag cccgtcgggc
2521 ctaccgatcc cagcatcgtg accctgatcg aagatagctc cgtcgtgacc agcggcgccc
2581 cccgccccac cttcaccggg accagcggct tcgacatcac cagcgccggc accaccaccc
2641 ccgccgtgct cgacattacc cccagcagca caagcgtcag catcagtacc acaaacttca
2701 caaaccccgc cttcagcgac cccagcatca tcgaggtgcc ccagaccggc gaagtcgccg
2761 gcaacgtgtt cgtgggcaca cccaccagcg gcacccacgg ctacgaagag atccccctgc
2821 agaccttcgc cagcagcggc accggcgaag agccaatctc ctccacaccc ctccccaccg
2881 tcagaagggt ggccggccca aggttgtatt cccgcgctta tcagcaagtc agcgtcgcca
2941 atcccgaatt cctgaccagg cccagcagcc tgatcaccta cgataacccc gctttcgaac
3001 ccgtcgatac caccctgacc ttcgacccca ggtccgacgt gcccgacagc gacttcatgg
3061 acatcattag gttgcaccgc cccgccctga ccagccgcag gggcaccgtg aggttctccc
3121 ggctgggcca gagagccacc atgttcacaa ggtctggcac ccagatcggc gcccgcgtgc
3181 atttctatca cgacatctcc cccatcgccc ccagccccga gtacatcgag ctccaacccc
3241 tcgtgagcgc taccgaagat aacgatctct tcgacatcta cgccgacgat atggatcccg
3301 ccgtccccgt gcccagcagg agcaccacaa gcttcgcctt cttcaagtac agcccaacca
3361 tcagcagcgc tagcagttac tccaacgtga ccgtgcccct gacaagtagc tgggacgtcc
3421 ccgtgtatac cggccccgac atcaccctgc ccagcaccac aagcgtgtgg ccaatcgtga
3481 gcccaaccgc tcccgctagc acccaataca tcggcatcca cggcacccac tactacctct
3541 ggcccctgta ctacttcatc cccaagaaga ggaagagggt gccatacttc ttcgccgacg
3601 ggttcgtcgc cgcttgagat cccccgggct gcaggaattc gatatcaagc ttatcgataa
3661 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
3721 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
3781 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
3841 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
3901 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
3961 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
4021 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
4081 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
4141 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
4201 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgtcg
4261 gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
4321 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
4381 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
4441 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
4501 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca
4561 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
4621 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
4681 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
4741 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
4801 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
4861 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
4921 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
4981 cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
5041 gcaggcagaa gtatgcaaag catgcagaat tctatcaaat atttaaagaa aaaaaaattg
5101 tatcaacttt ctacaatctc tttcagaaga cagaagcaga gggaatactt cctaaatcat
5161 tcaactaggc cagcattacc ttaataccgg aactagaaaa tgacattaca agaaaagaaa
5221 acaacagacc aatatctctc atgaacaaag atacaaacat tttcaacaaa atattagcaa
5281 aaagaatcca agaatgtatc aaaaaatata caccacaacc aagtagaatt tattccagat
5341 atgtaagggt ggttcaacgt ttgaaaatca attaacgtaa tttgtcccat caacaggtta
5401 aagaagaaaa tcacatggtc atattgatag acacagaaaa agcatttgac aaaatttaac
5461 acccattcat gatgcaatct ctcagtaaac taggaataga ggaaaacttc ctcagcttga
5521 atgtaccttc ctctcaattt tgctatgaac ctgaaactcc tcttaaaaaa taaagttttt
5581 catttaaaaa gaaaacaaaa aacatggagg agcgttgatg tatctcattt tagaccaatc
5641 agctatggat agttaggcga cagcacagat agctgctgta cttctgtttc tggcaatgtt
5701 ccagactaca tttaaaaaat ttttaattat agacttgtac ttaatgttca agaaaaatat
5761 gaaaatggct ttgccgtgtt aatgctactc ttttttaaaa aaaactaaag ttcaaacttt
5821 atttatattt cattagtttt ttagctactg ttctttttct gttctgggat ctcattcaga
5881 atgccacatt acatataatt ctcatgtctc cttgggttcc tcttagtttt gacagttcct
5941 cagacttttc ttatttttga tgaccttgac agttttgagg agtactggtt agatataggg
6001 taatggtttt taaagtatat ttgtcatgat ttatactggg gtaagggttt ggggaggaag
6061 cccatggggt aaagtactgt tctcatcaca tcatatcaag gttatatacc atcaatattg
6121 ccacagatgt tacttagcct tttaatattt ctctaattta gtgtatatgc aatgatagtt
6181 ctctgatttc tgagattgag tttctcatgt gtaatgatta tttagagttt ctctttcatc
6241 tgttcaaatt tttgtctagt tttatttttt actgatttgt aagacttctt tttataatct
6301 gcatattaca attctcttta ctggggtgtt gcaaatattt tctgtcattc tatggcctga
6361 cttttcttaa tggtttttta attttaaaaa taagtcttaa tattcatgca atctaattaa
6421 caatcttttc tttgtggtta ggactttgag tcataagaaa tttttctcta cactgaagtc
6481 atgatggcat gcttctatat tattttctaa aagatttaaa gttttgcctt ctccatttag
6541 acttataatt cactggaatt tttttgtgtg tatggtatga catatgggtt cccttttatt
6601 ttttacatat aaatatattt ccctgttttt ctaaaaaaga aaaagatcat cattttccca
6661 ttgtaaaatg ccatattttt ttcataggtc acttacatat atcaatgggt ctgtttctga
6721 gctctactct attttatcag cctcactgtc tatccccaca catctcatgc tttgctctaa
6781 atcttgatat ttagtggaac attctttccc attttgttct acaagaatat ttttgttatt
6841 gtcttttggg cttctatata cattttagaa tgaggttggc aagttaacaa acagcttttt
6901 tggggtgaac atattgacta caaatttatg tggaaagaaa gtaccaagtt gaccagtgcc
6961 gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg agttctggac cgaccggctc
7021 gggttctccc gggacttcgt ggaggacgac ttcgccggtg tggtccggga cgacgtgacc
7081 ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca acaccctggc ctgggtgtgg
7141 gtgcgcggcc tggacgagct gtacgccgag tggtcggagg tcgtgtccac gaacttccgg
7201 gacgcctccg ggccggccat gaccgagatc ggcgagcagc cgtgggggcg ggagttcgcc
7261 ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg aggagcagga ctgacacgtg
7321 ctacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
7381 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
7441 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
7501 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
7561 tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag
7621 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
7681 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
7741 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
7801 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
7861 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
7921 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
7981 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
8041 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
8101 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
8161 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
8221 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
8281 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
8341 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
8401 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
8461 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
8521 tgatccggca aacaaaccac cgctggtagc ggtttttttg tttgcaagca gcagattacg
8581 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
8641 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
8701 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
8761 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
8821 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
8881 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
8941 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
9001 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
9061 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
9121 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
9181 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
9241 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
9301 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
9361 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
9421 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
9481 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
9541 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
9601 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
9661 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
9721 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga
9781 gatctcccga tcccctatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
9841 gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt
9901 aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc
9961 gttttgcgct gcttcgcgat gtacgggcca gatatacgcg ttgacattga ttattgacta
10021 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
10081 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
10141 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
10201 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
10261 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
10321 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
10381 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
10441 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggaaccaa aatcaacggg
10501 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
10561 ggtgggaggt ctatataagc agagctctcc ctatcagtga tagagatctc cctatcagtg
10621 atagagatcg tcgacgagct cgtttagtga accgtcagat cgcctggaga cgccatccac
10681 gctgttttga cctccataga agacaccggg accgatccag cct
//
