| 质粒名称: | TagBFP2-CAAX |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 116856 |
| 存储实验室: | Gerry Hammonda |
|
载体骨架基本信息 |
|
| 载体名称: | pTagBFP2-C1 |
| 载体出品公司: | Clontech |
| 空载体大小: | 4758bp |
| 完整质粒大小: | 4758bp |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
|
载体抗性:LOCUS TagBFP2-CAAX 4758 bp DNA circular SYN 14-APR-2025
DEFINITION plasma membrane marker.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4758)
AUTHORS Goulden BD, Pacheco J, Dull A, Zewe JP, Deiters A, Hammond GRV
TITLE A high-avidity biosensor reveals plasma membrane PI(3,4)P2 is
predominantly a class I PI3K signaling product.
JOURNAL J Cell Biol. 2019 Mar 4;218(3):1066-1079. doi:
10.1083/jcb.201809026. Epub 2018 Dec 27.
PUBMED 30591513
REFERENCE 2 (bases 1 to 4758)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; doi:
"10.1083/jcb.201809026"; journalName: "J Cell Biol"; date:
"2019-03-4- 4"; volume: "218"; issue: "3"; pages: "1066-1079"
COMMENT Sequence Label: TagBFP2-CAAX
FEATURES Location/Qualifiers
source 1..4758
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 37..394
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
enhancer 37..228
/label=SV40 enhancer
/note="enhancer for the SV40 early promoter (Herr, 1993)"
primer_bind complement(60..80)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 245..380
/label=SV40 ori
/note="SV40 origin of replication"
CDS 429..1223
/codon_start=1
/product="aminoglycoside phosphotransferase"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
CDS 429..1223
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(483..502)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 1093..1112
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(1411..1430)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 1455..1502
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 1831..2419
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 2320..2339
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
enhancer 2594..2897
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 2898..3101
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 3052..3072
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
regulatory 3140..3149
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 3146..3847
/codon_start=1
/product="monomeric blue fluorescent protein"
/label=TagBFP
/note="mammalian codon-optimized"
/translation="MVSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIK
VVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
ATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMAL
KLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARY
CDLPSKLGHKLN"
polyA_signal 4079..4200
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(4116..4135)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 4170..4189
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(4207..4662)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(4344..4365)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 4556..4575
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter join(4689..4758,1..35)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gataaatgct tcaataatat tgaaaaagga agagtcctga ggcggaaaga accagctgtg
61 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
121 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
181 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
241 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
301 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
361 aggaggcttt tttggaggcc taggcttttg caaagatcga tcaagagaca ggatgaggat
421 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
481 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
541 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
601 atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
661 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
721 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
781 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
841 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
901 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca
961 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
1021 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
1081 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
1141 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
1201 gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac
1261 gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
1321 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
1381 gttcttcgcc caccctaggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg
1441 aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt
1501 tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca
1561 ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga
1621 aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcct caggttactc
1681 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
1741 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
1801 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
1861 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
1921 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
1981 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
2041 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
2101 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
2161 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
2221 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
2281 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
2341 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
2401 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
2461 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
2521 taccgccatg cattagttat taatagtaat caattacggg gtcattagtt catagcccat
2581 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
2641 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
2701 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
2761 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
2821 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
2881 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
2941 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
3001 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
3061 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tggtttagtg aaccgtcaga
3121 tccgctagcg ctaccggtcg ccaccatggt gagcgagctg attaaggaga acatgcacat
3181 gaagctgtac atggagggca ccgtggacaa ccatcacttc aagtgcacat ccgagggcga
3241 aggcaagccc tacgagggca cccagaccat gagaatcaag gtggtcgagg gcggccctct
3301 ccccttcgcc ttcgacatcc tggctactag cttcctctac ggcagcaaga ccttcatcaa
3361 ccacacccag ggcatccccg acttcttcaa gcagtccttc cctgagggct tcacatggga
3421 gagagtcacc acatacgaag acgggggcgt gctgaccgct acccaggaca ccagcctcca
3481 ggacggctgc ctcatctaca acgtcaagat cagaggggtg aacttcacat ccaacggccc
3541 tgtgatgcag aagaaaacac tcggctggga ggccttcacc gagacgctgt accccgctga
3601 cggcggcctg gaaggcagaa acgacatggc cctgaagctc gtgggcggga gccatctgat
3661 cgcaaacgcc aagaccacat atagatccaa gaaacccgct aagaacctca agatgcctgg
3721 cgtctactat gtggactaca gactggaaag aatcaaggag gccaacaacg agacctacgt
3781 cgagcagcac gaggtggcag tggccagata ctgcgacctc cctagcaaac tggggcacaa
3841 gcttaattcc ggactcagat ctcgagctca agcttcgaat tctgcagtcg acaaccctcc
3901 tgatgagagt ggccccggct gcatgagctg caagtgtgtg ctctcctgag gatccaccgg
3961 atctagataa ctgatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
4021 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
4081 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
4141 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
4201 acgcgtaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
4261 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
4321 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
4381 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
4441 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
4501 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
4561 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
4621 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc acttttcggg
4681 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
4741 tcatgagaca ataaccct
//
|
|
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | HRAS |
| 基因突变: | Amino acids 172-189 |
| GenBank编号 | JN247409 |
| 启动子: | CMV |
| 标签/融合蛋白: | TagBFP2 (N terminal on insert) |
| 克隆方法: | Gibson Cloning |
| 5’测序引物 | CMV-F |
| 3’测序引物 | SV40-pArev |
|
文献引用方法 |
|
| 材料和方法部分: | TagBFP2-CAAX was a gift from Gerry Hammond (Addgene plasmid # 116856 ; http://n2t.net/addgene:116856 ; RRID:Addgene_116856) |
| 参考文献部分: | A high-avidity biosensor reveals plasma membrane PI(3,4)P2 is predominantly a class I PI3K signaling product. Goulden BD, Pacheco J, Dull A, Zewe JP, Deiters A, Hammond GRV. J Cell Biol. 2019 Mar 4;218(3):1066-1079. doi: 10.1083/jcb.201809026. Epub 2018 Dec 27. 10.1083/jcb.201809026 PubMed 30591513 |
买家导航
TagBFP2-CAAX载体质粒基本信息
TagBFP2-CAAX质粒图谱载体图谱和TagBFP2-CAAX载体序列质粒序列多克隆位点信息
TagBFP2-CAAX质粒载体简介
TagBFP2-CAAX质粒序列载体序列
LOCUS TagBFP2-CAAX 4758 bp DNA circular SYN 14-APR-2025
DEFINITION plasma membrane marker.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4758)
AUTHORS Goulden BD, Pacheco J, Dull A, Zewe JP, Deiters A, Hammond GRV
TITLE A high-avidity biosensor reveals plasma membrane PI(3,4)P2 is
predominantly a class I PI3K signaling product.
JOURNAL J Cell Biol. 2019 Mar 4;218(3):1066-1079. doi:
10.1083/jcb.201809026. Epub 2018 Dec 27.
PUBMED 30591513
REFERENCE 2 (bases 1 to 4758)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; doi:
"10.1083/jcb.201809026"; journalName: "J Cell Biol"; date:
"2019-03-4- 4"; volume: "218"; issue: "3"; pages: "1066-1079"
COMMENT Sequence Label: TagBFP2-CAAX
FEATURES Location/Qualifiers
source 1..4758
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 37..394
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
enhancer 37..228
/label=SV40 enhancer
/note="enhancer for the SV40 early promoter (Herr, 1993)"
primer_bind complement(60..80)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 245..380
/label=SV40 ori
/note="SV40 origin of replication"
CDS 429..1223
/codon_start=1
/product="aminoglycoside phosphotransferase"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
CDS 429..1223
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(483..502)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 1093..1112
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(1411..1430)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 1455..1502
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 1831..2419
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 2320..2339
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
enhancer 2594..2897
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 2898..3101
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 3052..3072
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
regulatory 3140..3149
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 3146..3847
/codon_start=1
/product="monomeric blue fluorescent protein"
/label=TagBFP
/note="mammalian codon-optimized"
/translation="MVSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIK
VVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
ATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMAL
KLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARY
CDLPSKLGHKLN"
polyA_signal 4079..4200
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(4116..4135)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 4170..4189
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(4207..4662)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(4344..4365)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 4556..4575
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter join(4689..4758,1..35)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gataaatgct tcaataatat tgaaaaagga agagtcctga ggcggaaaga accagctgtg
61 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
121 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
181 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
241 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
301 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
361 aggaggcttt tttggaggcc taggcttttg caaagatcga tcaagagaca ggatgaggat
421 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
481 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
541 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
601 atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
661 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
721 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
781 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
841 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
901 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca
961 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
1021 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
1081 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
1141 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
1201 gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac
1261 gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
1321 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
1381 gttcttcgcc caccctaggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg
1441 aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt
1501 tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca
1561 ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga
1621 aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcct caggttactc
1681 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
1741 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
1801 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
1861 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
1921 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
1981 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
2041 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
2101 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
2161 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
2221 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
2281 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
2341 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
2401 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
2461 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
2521 taccgccatg cattagttat taatagtaat caattacggg gtcattagtt catagcccat
2581 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
2641 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
2701 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
2761 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
2821 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
2881 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
2941 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
3001 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
3061 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tggtttagtg aaccgtcaga
3121 tccgctagcg ctaccggtcg ccaccatggt gagcgagctg attaaggaga acatgcacat
3181 gaagctgtac atggagggca ccgtggacaa ccatcacttc aagtgcacat ccgagggcga
3241 aggcaagccc tacgagggca cccagaccat gagaatcaag gtggtcgagg gcggccctct
3301 ccccttcgcc ttcgacatcc tggctactag cttcctctac ggcagcaaga ccttcatcaa
3361 ccacacccag ggcatccccg acttcttcaa gcagtccttc cctgagggct tcacatggga
3421 gagagtcacc acatacgaag acgggggcgt gctgaccgct acccaggaca ccagcctcca
3481 ggacggctgc ctcatctaca acgtcaagat cagaggggtg aacttcacat ccaacggccc
3541 tgtgatgcag aagaaaacac tcggctggga ggccttcacc gagacgctgt accccgctga
3601 cggcggcctg gaaggcagaa acgacatggc cctgaagctc gtgggcggga gccatctgat
3661 cgcaaacgcc aagaccacat atagatccaa gaaacccgct aagaacctca agatgcctgg
3721 cgtctactat gtggactaca gactggaaag aatcaaggag gccaacaacg agacctacgt
3781 cgagcagcac gaggtggcag tggccagata ctgcgacctc cctagcaaac tggggcacaa
3841 gcttaattcc ggactcagat ctcgagctca agcttcgaat tctgcagtcg acaaccctcc
3901 tgatgagagt ggccccggct gcatgagctg caagtgtgtg ctctcctgag gatccaccgg
3961 atctagataa ctgatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
4021 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
4081 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
4141 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
4201 acgcgtaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
4261 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
4321 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
4381 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
4441 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
4501 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
4561 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
4621 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc acttttcggg
4681 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
4741 tcatgagaca ataaccct
//
