| 出品公司: | ATCC |
|---|---|
| 载体名称: | pC194 |
| 产品编号: | 37034 |
| 生长条件： | 37°C |
| 生物安全等级: | 1级 |
| 宿主菌: | 金黄色葡萄球菌芽孢杆菌 |
| 载体类型: | 质粒 |
| 复制子: | pC194 |
| 抗性: |
氯霉素
|
| 启动子: | lamdaPL |
| 建议宿主菌: | |
| 载体大小（kb）: | 3 |
| 多克隆位点: | 否 |
| 备注： |
自然隔离
用于芽孢杆菌克隆的通用质粒载体。
|
| 参考文献: |
P32
Ehrlich, S. D. 1978. DNA cloning in Bacillus subtilis. Proc. Natl. Acad. Sci. U. S. A. 75: 1433-1436.
P449
Gryczan TJ, et al. 1978. Characterization of Staphylococcus aureus plasmids introduced by transformation into Bacillus subtilis. J. Bacteriol. 134: 318-329.
P450
Horinouchi S, Weisblum B. 1982. Nucleotide sequence and functional map of pC194, a plasmid that specifies inducible chloramphenicol resistance. J. Bacteriol. 150: 815-825.
|
pC194载体序列
LOCUS pC194 2910 bp DNA circular CON 14-NOV-2021
DEFINITION Staphylococcus aureus plasmid pC194, complete sequence.
ACCESSION NC_002013 J01754
VERSION NC_002013.1
DBLINK BioProject: PRJNA224116
BioSample: SAMN14229320
KEYWORDS RefSeq; chloramphenicol resistance; circular; drug resistance gene;
transferase; unidentified reading frame.
SOURCE Staphylococcus aureus
ORGANISM Staphylococcus aureus
Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
Staphylococcus.
REFERENCE 1 (bases 1 to 2910)
AUTHORS Horinouchi,S. and Weisblum,B.
TITLE Nucleotide sequence and functional map of pC194, a plasmid that
specifies inducible chloramphenicol resistance
JOURNAL J Bacteriol 150 (2), 815-825 (1982)
PUBMED 6950931
COMMENT REFSEQ INFORMATION: The reference sequence is identical to
V01277.1.
The annotation was added by the NCBI Prokaryotic Genome Annotation
Pipeline (PGAP). Information about PGAP can be found here:
https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
##Genome-Annotation-Data-START##
Annotation Provider :: NCBI RefSeq
Annotation Date :: 11/14/2021 19:04:37
Annotation Pipeline :: NCBI Prokaryotic Genome
Annotation Pipeline (PGAP)
Annotation Method :: Best-placed reference protein
set; GeneMarkS-2+
Annotation Software revision :: 5.3
Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA;
repeat_region
Genes (total) :: 2
CDSs (total) :: 2
Genes (coding) :: 0
CDSs (with protein) :: 0
Genes (RNA) :: 0
tRNAs :: 0
ncRNAs :: 0
Pseudo Genes (total) :: 2
CDSs (without protein) :: 2
Pseudo Genes (ambiguous residues) :: 0 of 2
Pseudo Genes (frameshifted) :: 2 of 2
Pseudo Genes (incomplete) :: 0 of 2
Pseudo Genes (internal stop) :: 0 of 2
##Genome-Annotation-Data-END##
COMPLETENESS: full length.
FEATURES Location/Qualifiers
source 1..2910
/organism="Staphylococcus aureus"
/mol_type="genomic DNA"
/db_xref="taxon:1280"
/plasmid="pC194"
gene complement(join(2773..2910,1..791))
/locus_tag="HXH75_RS00005"
/old_locus_tag="pC194_p1"
/pseudo
CDS complement(join(2773..2910,1..791))
/locus_tag="HXH75_RS00005"
/old_locus_tag="pC194_p1"
/inference="COORDINATES: similar to AA
sequence:RefSeq:WP_002499696.1"
/note="frameshifted; Derived by automated computational
analysis using gene prediction method: Protein Homology."
/pseudo
/codon_start=1
/transl_table=11
/product="protein rep"
gene 1260..1979
/gene="catA"
/locus_tag="HXH75_RS00010"
/old_locus_tag="pC194_p3"
/pseudo
CDS 1260..1979
/gene="catA"
/locus_tag="HXH75_RS00010"
/old_locus_tag="pC194_p3"
/inference="COORDINATES: similar to AA
sequence:RefSeq:WP_032488880.1"
/note="frameshifted; Derived by automated computational
analysis using gene prediction method: Protein Homology."
/pseudo
/codon_start=1
/transl_table=11
/product="type A chloramphenicol O-acetyltransferase"
ORIGIN
1 gatcaatttc ttttaagtaa tctaaatccc cattttttaa tttcttttta gcctctttaa
61 ataatcctga ataaactaat acctgtttac ctttaagtga tttataaaat gcatcaaaga
121 ctttttgatt tatttattaa ataatcacta tctttaccag aatacttagc catttcatat
181 aattctttat tattattttg tcttattttt tgaacttgaa cttgtgttat ttctgaaatg
241 cccgttacat cacgccataa atctaaccat tcttgttggc taatataata tcttttatct
301 gtgaaatacg atttatttac tgcaattaac acatgaaaat gaggattata atcatctctt
361 tttttattat atgtaatctc taacttacga acatatccct ttataacact acctactttt
421 tttctcttta taagttttct aaaagaatta ttataacgtt ttatttcatt ttctaattca
481 tcactcatta cattaggtgt agtcaaagtt aaaaagataa actccttttt ctcttgctgc
541 ttaatatatt gcatcatcaa agataaaccc aatgcatctt ttctagcttt tctccaagca
601 cagacaggac aaaatcgatt tttacaagaa ttagctttat ataatttctg tttttctaaa
661 gttttatcag ctacaaaaga cagaaatgta ttgcaatctt caactaaatc catttgattc
721 tctccaatat gacgtttaat aaatttctga aatacttgat ttctttgttt tttctcagta
781 tacttttcca tgttataaca cataaaaaca acttagtttt cacaaactat gacaataaaa
841 aaagttgctt tttccccttt ctatgtatgt tttttactag tcatttaaaa cgatacatta
901 ataggtacga aaaagcaact ttttttgcgc ttaaaaccag tcataccaat aacttaaggg
961 taactagcct cgccggcaat agttaccctt attatcaaga taagaaagaa aaggattttt
1021 cgctacgctc aaatccttta aaaaaacaca aaagaccaca ttttttaatg tggtctttat
1081 tcttcaacta aagcacccat tagttcaaca aacgaaaatt ggataaagtg ggatattttt
1141 aaaatatata tttatgttac agtaatattg acttttaaaa aaggattgat tctaatgaag
1201 aaagcagaca agtaagcctc ctaaattcac tttagataaa aatttaggag gcatatcaaa
1261 tgaactttaa taaaattgat ttagacaatt ggaagagaaa agagatattt aatcattatt
1321 tgaaccaaca aacgactttt agtataacca cagaaattga tattagtgtt ttataccgaa
1381 acataaaaca agaaggatat aaattttacc ctgcatttat tttcttagtg acaagggtga
1441 taaactcaaa tacagctttt agaactggtt acaatagcga cggagagtta ggttattggg
1501 ataagttaga gccactttat acaatttttg atggtgtatc taaaacattc tctggtattt
1561 ggactcctgt aaagaatgac ttcaaagagt tttatgattt atacctttct gatgtagaga
1621 aatataatgg ttcggggaaa ttgtttccca aaacacctat acctgaaaat gctttttctc
1681 tttctattat tccatggact tcatttactg ggtttaactt aaatatcaat aataatagta
1741 attaccttct acccattatt acagcaggaa aattcattaa taaaggtaat tcaatatatt
1801 taccgctatc tttacaggta catcattctg tttgtgatgg ttatcatgca ggattgttta
1861 tgaactctat tcaggaattg tcagataggc ctaatgactg gcttttataa tatgagataa
1921 tgccgactgt actttttaca gtcggttttc taatgtcact aacctgcccc gttagttgaa
1981 gaaggttttt atattacagc tccagatcca tatccttctt tttctgaacc gacttctcct
2041 ttttcgcttc tttattccaa ttgctttatt gacgttgagc ctcggaaccc ttaacaatcc
2101 caaaacttgt cgaatggtcg gcttaatagc tcacgctatg ccgacattcg tctgcaagtt
2161 tagttaaggg ttcttctcaa cgcacaataa attttctcgg cataaatgcg tggtctaatt
2221 tttattttta ataaccttga tagcaaaaaa tgccattcca atacaaaacc acatacctat
2281 aatcgataac cacataacag tcataaaacc actccttttt aacaaacttt atcacaagaa
2341 atatttaaat tttaaatgcc tttattttga attttaaggg gcattttaaa gatttagggg
2401 taaatcatat agttttatgc ctaaaaacct acagaagctt ttaaaaagca aatatgagcc
2461 aaataaatat attctaattc tacaaacaaa aatttgagca aattcagtgt cgatttttta
2521 agacactgcc cagttacatg caaattaaaa ttttcatgat tttttatagt tcctaacagg
2581 gttaaaattt gtataacgaa agtataatgt ttatataacg ttagtataat aaagcatttt
2641 aacattatac ttttgataat cgtttatcgt cgtcatcaca ataactttta aaatactcgt
2701 gcataattca cgctgacctc ccaataacta catggtgtta tcgggaggtc agctgttagc
2761 acttatattt tgttattgtt cttcctcgat ttcgtctatc attttgtgat taatttctct
2821 tttttcttgt tctgttaagt cataaagttc actagctaaa tactcttttt gtttccaaat
2881 ataaaaaatt tgatagatat attacggttg
//
