| 质粒名称: | pAAV-CaMKII-ElectraON-Kv2.1 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000565 |
| 产品用途: | to express ElectraON-Kv2.1 in neurons |
| 存储实验室: | KIRYL PIATKEVICH Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV |
| 表达系统: | 腺病毒，哺乳动物表达 |
| 启动子: | CaMKII |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | ElectraON-Kv2.1 |
| 物种来源: | mouse |
| 密码子优化: | 老鼠表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Bright and Photostable Voltage Sensors Derived from mBaoJin
Zhang H., Zhou S., Kuzmicheva T.P., Subach O.M., Grimm C., Eom M., Baror-Sebban S., Adam Y., Yoon Y.G., Emilliani V., Subach F.V., Piatkevich K.D.
bioRxiv 2025.05.30.657123 (2025)
|
买家导航
pAAV-CaMKII-ElectraON-Kv2.1载体质粒基本信息
pAAV-CaMKII-ElectraON-Kv2.1质粒图谱载体图谱和pAAV-CaMKII-ElectraON-Kv2.1载体序列质粒序列多克隆位点信息
pAAV-CaMKII-ElectraON-Kv2.1质粒载体简介
pAAV-CaMKII-ElectraON-Kv2.1质粒序列载体序列
LOCUS pAAV-CaMKII-ElectraON-Kv2.1 6729 bp DNA circular SYN 24-JUN-2024
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6729)
AUTHORS Kiryl Piatkevich
TITLE Direct Submission
JOURNAL Exported Apr 30, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6729
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 34..223
/label=ITR
promoter 237..1526
/label=CaMKII promoter
/note="promoter for the mouse calcium/calmodulin-dependent
kinase II alpha subunit"
regulatory 1539..1548
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
misc_feature 1539..1544
/label=Kozak sequence
CDS 1545..2231
/codon_start=1
/label=AcePR
/translation="MADVETETGMIAQWIVFAIMAAAAIAFGVAVHFRPSELKSAYYIN
IAICTIAATAYYAMAVNYQDLTMNGERQVVYAKYIDWVLTTPLLLLNLIVMTKMGGVMI
SWVIGADIFMIVFGILGAFEDEHKFKWVYFIAGCVMQAVLTYGMYNATWKDDLKKSPEY
HSSYVSLLVFLSILFVFYPVVWAFGSGSGVLSVDNEAILMGILDVLAKPLFGMGCLIAH
ETIFKSY"
regulatory 1545..1548
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 2235..2903
/codon_start=1
/label=mBaoJin2(codon optimized)
/translation="STPFKFQLKGTINGKSFTVEGEGEGNSHEGSHKGKYVCTSGKLPM
SWAALGTSFGYGMKYYTKYPSGLKNWFREVMPGGFTYDRHIQYKGDGSIHAKHQHFMKN
GTYHNIVEFTGQDFKENSPVLTGDMNVSLPNEVPHIPRDDGVECPVTLLYPLLSDKSKY
VEAYQYTICKPLHNQPAPDVPYHWIRKQYTQSKDDAEERDHICQSETLEAHLKGMDELY
K"
CDS 2904..2963
/codon_start=1
/label=KGC
/translation="KSRITSEGEYIPLDQIDINV"
CDS 2964..2984
/codon_start=1
/label=ER2
/translation="FCYENEV"
CDS 2985..3179
/codon_start=1
/label=Kv2.1
/translation="QSQPILNTKEMAPQSKPPEELEMSSMPSPVAPLPARTEGVIDMRS
MSSIDSFISCATDFPEATRF"
primer_bind complement(3160..3208)
/label=1104-3-Kv2.1-R
primer_bind complement(3160..3181)
/label=Kv2.1-Rv-ER2
misc_feature 3207..3795
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
CDS complement(3678..3689)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
polyA_signal 3838..3959
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
misc_feature complement(4062..4251)
/label=ITR
misc_feature 4305..5245
/label=ColE1
/note="ColE1"
CDS complement(5246..6106)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(6107..6211)
/gene="bla"
/label=AmpR promoter
rep_origin complement(6237..6692)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
ORIGIN
1 gcgatcggtg cgggcctctt cgctattacg ccagctgcgc gctcgctcgc tcactgaggc
61 cgcccgggca aagcccgggc gtcgggcgac ctttggtcgc ccggcctcag tgagcgagcg
121 agcgcgcaga gagggagtgg ccaactccat cactaggggt tccttgtagt taatgattaa
181 cccgccatgc tacttatcta cgtagccatg ctctaggaag atcgtaggtt aattaacatt
241 atggccttag gtcacttcat ctccatgggg ttcttcttct gattttctag aaaatgagat
301 gggggtgcag agagcttcct cagtgacctg cccagggtca catcagaaat gtcagagcta
361 gaacttgaac tcagattact aatcttaaat tccatgcctt gggggcatgc aagtacgata
421 tacagaagga gtgaactcat tagggcagat gaccaatgag tttaggaaag aagagtccag
481 ggcagggtac atctacacca cccgcccagc cctgggtgag tccagccacg ttcacctcat
541 tatagttgcc tctctccagt cctaccttga cgggaagcac aagcagaaac tgggacagga
601 gccccaggag accaaatctt catggtccct ctgggaggat gggtggggag agctgtggca
661 gaggcctcag gaggggccct gctgctcagt ggtgacagat aggggtgaga aagcagacag
721 agtcattccg tcagcattct gggtctgttt ggtacttctt ctcacgctaa ggtggcggtg
781 tgatatgcac aatggctaaa aagcagggag agctggaaag aaacaaggac agagacagag
841 gccaagtcaa ccagaccaat tcccagagga agcaaagaaa ccattacaga gactacaagg
901 gggaagggaa ggagagatga attagcttcc cctgtaaacc ttagaaccca gctgttgcca
961 gggcaacggg gcaatacctg tctcttcaga ggagatgaag ttgccagggt aactacatcc
1021 tgtctttctc aaggaccatc ccagaatgtg gcacccacta gccgttacca tagcaactgc
1081 ctctttgccc cacttaatcc catcccgtct gttaaaaggg ccctatagtt ggaggtgggg
1141 gaggtaggaa gagcgatgat cacttgtgga ctaagtttgt tcacatcccc ttctccaacc
1201 ccctcagtac atcaccctgg gagaacaagg tccacttgct tctgggccca cacagtcctg
1261 cagtattgtg tatataaggc cagggcaacg gaggagcagg ttttgaagtg aaaggcaggc
1321 aggtgttggg gaggcagtta ccggggcaac gggaacaggg cgtttcggag gtggttgcca
1381 tggggacctg gatgctgacg aaggctcgcg aggctgtgag cagccacagt gccctgctca
1441 gaagccccaa gctcgtcaat caagctggtt ctccatttgc actcaggagc acgggcaggc
1501 gagtggcccc tagttctggg ggcagcgggg gatcctaagc caccatggct gacgtggaaa
1561 ccgagaccgg catgattgca cagtggattg tctttgctat tatggctgct gctgctattg
1621 cttttggagt ggctgtgcac tttcggcctt cagagctgaa gagcgcatac tatatcaaca
1681 ttgccatctg cactatcgcc gctaccgctt actatgcaat ggccgtgaac taccaggacc
1741 tgacaatgaa tggtgaaagg caggtggtct acgcaaagta tattgactgg gtgctgacca
1801 caccactgct cctgctcaac ctcatcgtca tgaccaagat gggcggagtg atgatttctt
1861 gggtcatcgg cgcagacatt ttcatgatcg tgtttggtat tctgggcgcc ttcgaggatg
1921 aacacaagtt caaatgggtg tactttatcg ctggatgtgt gatgcaggca gtcctgacat
1981 acgggatgta taacgccact tggaaagacg atctgaagaa aagccccgag taccatagct
2041 cctatgtcag tctgctcgtc ttcctgtcaa tcctcttcgt gttttatcct gtcgtgtggg
2101 ctttcgggtc tggtagtggc gtgctgtccg tcgacaatga ggccattctc atgggaatcc
2161 tggatgtgct cgctaagcca ctgtttggaa tggggtgcct cattgcccat gagactatct
2221 tcaagtccta cgccagtact cccttcaagt ttcagctcaa agggaccatc aatgggaaga
2281 gcttcactgt tgagggagag ggcgaaggaa actctcatga gggttctcac aaggggaaat
2341 atgtctgcac atctggaaag cttcccatgt catgggcagc cttgggcaca agtttcggct
2401 atggcatgaa gtactacacg aaatacccat ccggtctgaa gaactggttt cgagaggtga
2461 tgcctggtgg ctttacctat gacaggcata tccagtacaa gggagatgga tccattcatg
2521 cgaagcacca gcacttcatg aagaatggga cgtaccacaa cattgtggag tttaccggtc
2581 aagacttcaa ggagaatagc ccggtgttga caggcgatat gaatgtgagc cttcccaacg
2641 aagtccctca catacctaga gatgatggcg ttgagtgtcc agtcacactc ctgtacccac
2701 tgctgagcga caagtccaag tatgtggaag cctatcagta caccatctgc aaaccgctgc
2761 acaatcaacc agctcctgat gtgccctatc actggattcg gaaacagtat actcagagca
2821 aagatgacgc agaagaacgc gaccatatct gtcagtcaga aaccctggaa gctcatctga
2881 aagggatgga cgagctctac aaaaagagca ggatcaccag cgagggcgag tacatccccc
2941 tggaccagat cgacatcaac gtgttctgct acgagaacga ggtgcagagc cagcctatcc
3001 tgaacaccaa ggagatggcc cctcagagca agcctcctga ggagctggag atgagcagca
3061 tgcctagccc tgtggcccct ctgcctgcca ggaccgaggg cgtgatcgac atgaggagca
3121 tgagcagcat cgacagcttc atcagctgcg ccaccgactt ccctgaggcc accaggttct
3181 aagaattcga tatcaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat
3241 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
3301 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
3361 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
3421 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
3481 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
3541 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
3601 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
3661 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
3721 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
3781 gggccgcctc cccgcatcga taccgtcgac ccgggcggcc gcttcgagca gacatgataa
3841 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
3901 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta
3961 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggagatgtgg gaggtttttt
4021 aaagcaagta aaacctctac aaatgtggta aaatcgataa ggatcttcct agagcatggc
4081 tacgtagata agtagcatgg cgggttaatc attaactaca aggaacccct agtgatggag
4141 ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc
4201 cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ctgcattaat
4261 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
4321 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
4381 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
4441 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
4501 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
4561 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
4621 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
4681 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
4741 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
4801 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
4861 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
4921 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
4981 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
5041 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
5101 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
5161 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
5221 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
5281 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
5341 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
5401 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
5461 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
5521 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
5581 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
5641 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
5701 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
5761 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
5821 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
5881 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
5941 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
6001 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
6061 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
6121 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
6181 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctaaat
6241 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
6301 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
6361 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
6421 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
6481 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
6541 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
6601 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
6661 cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc tgcgcaactg
6721 ttgggaagg
//
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