| 质粒名称: | pAAV-hSyn-GRAB_HA1h |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000149 |
| 产品用途: | Express the high-affinity histamine sensor GRAB_HA1h in neurons |
| 存储实验室: | Yulong Li Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV |
| 表达系统: | 腺病毒，哺乳动物表达 |
| 启动子: | hSyn |
| 拷贝数: | 低拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a，TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | GRAB_HA1h |
| 物种来源: | Synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Genetically encoded sensors for measuring histamine release both in vitro and in vivo
Dong H., Li M., Yan Y., Qian T., Lin Y., Ma X., Vischer H.F., Liu C., Li G., Wang H., Leurs R., Li Y.
Neuron 111, 1564-1576.e6 (2023)
|
买家导航
pAAV-hSyn-GRAB_HA1h载体质粒基本信息
pAAV-hSyn-GRAB_HA1h质粒图谱载体图谱和pAAV-hSyn-GRAB_HA1h载体序列质粒序列多克隆位点信息
pAAV-hSyn-GRAB_HA1h质粒载体简介
pAAV-hSyn-GRAB_HA1h质粒序列载体序列
LOCUS pAAV-hSyn-GRAB_HA1h 6278 bp DNA circular SYN 21-APR-2023
DEFINITION Express the high-affinity histamine sensor GRAB_HA1h in neurons.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6278)
AUTHORS Dong H, Li M, Yan Y, Qian T, Lin Y, Ma X, Vischer HF, Liu C, Li G,
Wang H, Leurs R, Li Y
TITLE Genetically encoded sensors for measuring histamine release both in
vitro and in vivo
JOURNAL bioRxiv 2022.08.19.504485
REFERENCE 2 (bases 1 to 6278)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 28, 2026 from SnapGene 6.0.2
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv
2022.08.19.504485"
FEATURES Location/Qualifiers
source 1..6278
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR (alternate)
/note="Functional equivalent of wild-type AAV2 ITR"
promoter 131..574
/label=hSyn
regulatory 581..590
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
sig_peptide 587..649
/codon_start=1
/product="leader sequence from mouse immunoglobulin kappa
light chain"
/label=Ig-kappa leader
/translation="METDTLLLWVLLLWVPGSTGD"
protein_bind 650..674
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
CDS 674..2380
/codon_start=1
/label=GRAB_HA1h
/translation="TPDTNSTINLSLSTRVTLAFFMSLVAFAIMLGNALVILAFVVDKN
LRHRSSYFFLNLAISDFFVGVISIPLYIPHTLFEWDFGKEICVFWLTTDYLLCTASVYN
IVLISYDRYLSVSNAVSYRTQHTGVLKIVTLMVAVWVLAFLVNGPMILVSESWKDEGSE
CEPGFFSEWYILAITSFLEFVIPVILVAYFNMNIYWSLWKRDHLSRCLPAPAGPRDTDA
LDLEEGGNVYIKADKQKNGIKANFVIRHNIEDGGVQLAYHYQQNTPIGDGPVLLPDNHY
LSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVRKGEELFTGVVPI
LVELDGDVNGHKFSVSGEGEGDATEGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSR
YPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKE
DGNILGHKLEYNTGAAARWRAAKSLAILLGVFAVCWAPYSLFTIVLSFYSSATGPKSVW
YRIAFWLQWFNSFVNPLLYPLCHKRFQKAFLKIFCIKKQPLPSQHSRSVSS"
primer_bind 1567..1588
/label=EGFP-C
/note="EGFP, forward primer"
primer_bind complement(1694..1715)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(1955..1974)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
misc_feature 2405..2993
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(2458..2478)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(2876..2887)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
polyA_signal 3025..3501
/label=hGH poly(A) signal
/note="human growth hormone polyadenylation signal"
primer_bind complement(3224..3243)
/label=hGH-PA-R
/note="Human growth hormone terminator, reverse primer"
repeat_region 3541..3681
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
repeat_region 3541..3670
/label=AAV2 ITR
rep_origin 3756..4211
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(3843..3862)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 4053..4074
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(4228..4247)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 4347..4369
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(4407..4425)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 4493..4597
/gene="bla"
/label=AmpR promoter
CDS 4598..5458
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(4816..4835)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 5629..6217
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 6118..6137
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtatgagg cggggtgggg gtgcctacct gacgaccgac
181 cccgacccac tggacaagca cccaaccccc attccccaaa ttgcgcatcc cctatcagag
241 agggggaggg gaaacaggat gcggcgaggc gcgtgcgcac tgccagcttc agcaccgcgg
301 acagtgcctt cgcccccgcc tggcggcgcg cgccaccgcc gcctcagcac tgaaggcgcg
361 ctgacgtcac tcgccggtcc cccgcaaact ccccttcccg gccaccttgg tcgcgtccgc
421 gccgccgccg gcccagccgg accgcaccac gcgaggcgcg agataggggg gcacgggcgc
481 gaccatctgc gctgcggcgc cggcgactca gcgctgcctc agtctgcggt gggcagcgga
541 ggagtcgtgt cgtgcctgag agcgcagtcg agaaaccggt gccaccatgg agacagacac
601 actcctgcta tgggtactgc tgctctgggt tccaggttcc actggtgaca caagtttgta
661 caaaaaagtt ggcaccccag atactaatag cacaatcaat ttatcactaa gcactcgtgt
721 tactttagca ttttttatgt ccttagtagc ttttgctata atgctaggaa atgctttggt
781 cattttagct tttgtggtgg acaaaaacct tagacatcga agtagttatt tttttcttaa
841 cttggccatc tctgacttct ttgtgggtgt gatctccatt cctttgtaca tccctcacac
901 gctgttcgaa tgggattttg gaaaggaaat ctgtgtattt tggctcacta ctgactatct
961 gttatgtaca gcatctgtat ataacattgt cctcatcagc tatgatcgat acctgtcagt
1021 ctcaaatgct gtgtcttata gaactcaaca tactggggtc ttgaagattg ttactctgat
1081 ggtggccgtt tgggtgctgg ccttcttagt gaatgggcca atgattctag tttcagagtc
1141 ttggaaggat gaaggtagtg aatgtgaacc tggatttttt tcggaatggt acatccttgc
1201 catcacatca ttcttggaat tcgtgatccc agtcatctta gtcgcttatt tcaacatgaa
1261 tatttattgg agcctgtgga agcgtgatca tctcagtagg tgcctgccag caccggccgg
1321 gccgcgcgac accgacgcgc tggacctgga ggagggagga aatgtctata tcaaggccga
1381 caagcagaag aacggcatca aggcgaactt cgtgatccgc cacaacatcg aggacggcgg
1441 cgtgcagctc gcctaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
1501 gcccgacaac cactacctga gcgtgcagtc caaactttcg aaagacccca acgagaagcg
1561 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
1621 gctgtacaag ggcggtaccg gagggagcat ggtgagaaag ggcgaggagc tgttcaccgg
1681 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
1741 cggcgagggt gagggcgatg ccaccgaggg caagctgacc ctgaagttca tctgcaccac
1801 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg
1861 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
1921 aggctacatc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
1981 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
2041 caaggaggac ggcaacatcc tggggcacaa gctggagtac aacaccggag cagcagcacg
2101 ctggcgcgcg gcgaagtcac tggccattct cttaggggtt tttgctgttt gctgggctcc
2161 atattctctg ttcacaattg tcctttcatt ttattcctca gcaacaggtc ctaaatcagt
2221 ttggtataga attgcatttt ggcttcagtg gttcaattcc tttgtcaatc ctcttttgta
2281 tccattgtgt cacaagcgct ttcaaaaggc tttcttgaaa atattttgta taaaaaagca
2341 acctctacca tcacaacaca gtcggtcagt atcttcttaa gaattcgata tcaagcttat
2401 cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
2461 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
2521 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
2581 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
2641 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
2701 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
2761 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
2821 gctcgcctat gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
2881 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
2941 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata
3001 ccgagcgctg ctcgagagat ctacgggtgg catccctgtg acccctcccc agtgcctctc
3061 ctggccctgg aagttgccac tccagtgccc accagccttg tcctaataaa attaagttgc
3121 atcattttgt ctgactaggt gtccttctat aatattatgg ggtggagggg ggtggtatgg
3181 agcaaggggc aagttgggaa gacaacctgt agggcctgcg gggtctattg ggaaccaagc
3241 tggagtgcag tggcacaatc ttggctcact gcaatctccg cctcctgggt tcaagcgatt
3301 ctcctgcctc agcctcccga gttgttggga ttccaggcat gcatgaccag gctcagctaa
3361 tttttgtttt tttggtagag acggggtttc accatattgg ccaggctggt ctccaactcc
3421 taatctcagg tgatctaccc accttggcct cccaaattgc tgggattaca ggcgtgaacc
3481 actgctccct tccctgtcct tctgattttg taggtaacca cgtgcggacc gagcggccgc
3541 aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg
3601 ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc
3661 gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt tctccttacg catctgtgcg
3721 gtatttcaca ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg gcgcattaag
3781 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccttagcgcc
3841 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
3901 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
3961 aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
4021 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
4081 actcaactct atctcgggct attcttttga tttataaggg attttgccga tttcggtcta
4141 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
4201 gtttacaatt ttatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
4261 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
4321 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
4381 atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
4441 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
4501 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
4561 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
4621 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
4681 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
4741 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
4801 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
4861 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
4921 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
4981 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
5041 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
5101 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
5161 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
5221 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
5281 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
5341 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
5401 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
5461 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
5521 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
5581 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
5641 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
5701 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
5761 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
5821 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
5881 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
5941 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
6001 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
6061 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
6121 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
6181 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
6241 acggttcctg gccttttgct ggccttttgc tcacatgt
//
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