pQL128

LOCUS       pQL128             8233 bp DNA     circular SYN 19-SEP-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 8233)
  AUTHORS   Westlake University
  TITLE     Direct Submission
  JOURNAL   Exported Sep 2, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..8233
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     primer_bind     186..207
                     /label=olyl419
     misc_feature    235..818
                     /label=P_CMV
                     /label=nonstandard type: promoter_const
     misc_feature    235..614
                     /label=P_CMV_enhancer
                     /label=nonstandard type: promoter_element
     misc_feature    615..818
                     /label=P_CMV_IE
                     /label=nonstandard type: promoter_element
     primer_bind     769..789
                     /label=oMHS-CMV-F
     misc_feature    829
                     /label=putative transcriptional start
     promoter        863..882
                     /label=T7 promoter
     primer_bind     complement(871..894)
                     /label=olyl418
     primer_bind     881..921
                     /label=QL319 NFAT4-F
     primer_bind     881..882
                     /label=T7
     misc_feature    901..3795
                     /label=NFAT4
     primer_bind     complement(3775..3815)
                     /label=QL320 NFAT4-R
     terminator      3830..4057
                     /label=bGH polyA
     rep_origin      4103..4531
                     /label=f1 bacteriophage origin of replication
                     /label=f1 bacteriophage origin of replication; arrow _f1 
                     ori
     misc_feature    4545..4874
                     /label=P_SV40
                     /label=nonstandard type: promoter_const
     rep_origin      4725..4860
                     /label=SV40 ORI
     misc_feature    4777..4826
                     /label=P_SV40 TSS-region
     CDS             4941..5735
                     /label=Translation 2136-2930
     misc_feature    4941..5732
                     /label=Kan/neoR
                     /label=nonstandard type: resistance
     primer_bind     complement(6075..6095)
                     /label=M13-rev
     misc_binding    complement(6101..6123)
                     /label=LacO
     promoter        complement(6127..6157)
                     /label=lac promoter
     rep_origin      complement(6481..7066)
                     /direction=LEFT
                     /label=high-copy-number ColE1/pMB1/pBR322/pUC origin o
                     /label=high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     _ori
     CDS             complement(7237..8097)
                     /label=Translation 5292-4432
     misc_feature    complement(7240..8097)
                     /label=AmpR
                     /label=nonstandard type: resistance
     promoter        complement(8098..8202)
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 atgataacca ccctcccatc tctcctaccc gccagcctcg ccagtatctc ccaccgagtc
      961 acgaatctcc catctaactc cctctcacac aacccaggcc tctccaagcc tgactttccc
     1021 ggaaactcca gtccaggtct tccttcctcc tccagcccag gccgggacct gggggctcct
     1081 gccggatcca tgggggcggc cagctgcgag gatgaggagc tggaatttaa gctggtgttc
     1141 ggggaggaaa aggaggcccc cccgctgggc gcggggggat tgggggaaga actggactca
     1201 gaggatgccc cgccatgctg ccgtctggcc ttgggagagc cccctcccta tggcgctgca
     1261 cctatcggta ttccccgacc tccaccccct cggcctggca tgcattcgcc accgccgcga
     1321 ccagccccct cacctggcac ctgggagagc cagcccgcca ggtcggtgag gctgggagga
     1381 ccaggagggg gtgctggggg tgctgggggt ggccgtgttc tcgagtgtcc cagcatccgc
     1441 atcacctcca tctctcccac gccggagccg ccagcagcgc tggaggacaa ccctgatgcc
     1501 tggggggacg gctctcctag agattacccc ccaccagaag gctttggggg ctacagagaa
     1561 gcagggggcc agggtggggg ggccttcttc agcccaagcc ctggcagcag cagcctgtcc
     1621 tcgtggagct tcttctccga tgcctctgac gaggcagccc tgtatgcagc ctgcgacgag
     1681 gtggagtctg agctaaatga ggcggcctcc cgctttggcc tgggctcccc gctgccctcg
     1741 ccccgggcct cccctcggcc atggaccccc gaagatccct ggagcctgta tggtccaagc
     1801 cccggaggcc gagggccaga ggatagctgg ctactcctca gtgctcctgg gcccacccca
     1861 gcctccccgc ggcctgcctc tccatgtggc aagcggcgct attccagctc gggaacccca
     1921 tcttcagcct ccccagctct gtcccgccgt ggcagcctgg gggaagaggg gtctgagcca
     1981 cctccaccac ccccattgcc tctggcccgg gacccgggct cccctggtcc ctttgactat
     2041 gtgggggccc caccagctga gagcatccct cagaagacac ggcggacttc cagcgagcag
     2101 gcagtggctc tgcctcggtc tgaggagcct gcctcatgca atgggaagct gcccttggga
     2161 gcagaggagt ctgtggctcc tccaggaggt tcccggaagg aggtggctgg catggactac
     2221 ctggcagtgc cctccccact cgcttggtcc aaggcccgga ttgggggaca cagccctatc
     2281 ttcaggacct ctgccctacc cccactggac tggcctctgc ccagccaata tgagcagctg
     2341 gagctgagga tcgaggtaca gcctagagcc caccaccggg cccactatga gacagaaggc
     2401 agccgtggag ctgtcaaagc tgcccctggc ggtcaccccg tagtcaagct cctaggctac
     2461 agtgagaagc cactgaccct acagatgttc atcggcactg cagatgaaag gaacctgcgg
     2521 cctcatgcct tctatcaggt gcaccgtatc acaggcaaga tggtggccac ggccagctat
     2581 gaagccgtag tcagtggcac caaggtgttg gagatgactc tgctgcctga gaacaacatg
     2641 gcggccaaca ttgactgcgc gggaatcctg aagcttcgga attcagacat tgagcttcgg
     2701 aagggtgaga cggacatcgg gcgcaaaaac acacgtgtac ggctggtgtt ccgggtacac
     2761 gtgccccagg gcggcgggaa ggtcgtctca gtacaggcag catcggtgcc catcgagtgc
     2821 tcccagcgct cagcccagga gctgccccag gtggaggcct acagccccag tgcctgctct
     2881 gtgagaggag gcgaggaact ggtactgact ggctccaact tcctgccaga ctccaaggtg
     2941 gtgttcattg agaggggtcc tgatgggaag ctgcaatggg aggaggaggc cacagtgaac
     3001 cgactgcaga gcaacgaggt gacgctgacc ctgactgtcc ccgagtacag caacaagagg
     3061 gtttcccggc cagtccaggt ctacttttat gtctccaatg ggcggaggaa acgcagtcct
     3121 acccagagtt tcaggtttct gcctgtgatc tgcaaagagg agcccctacc ggactcatct
     3181 ctgcggggtt tcccttcagc atcggcaacc ccctttggca ctgacatgga cttctcacca
     3241 cccaggcccc cctacccctc ctatccccat gaagaccctg cttgcgaaac tccttaccta
     3301 tcagaaggct tcggctatgg catgccccct ctgtaccccc agacggggcc cccaccatcc
     3361 tacagaccgg gcctgcggat gttccctgag actaggggta ccacaggttg tgcccaacca
     3421 cctgcagttt ccttccttcc ccgccccttc cctagtgacc cgtatggagg gcggggctcc
     3481 tctttctccc tggggctgcc attctctccg ccagccccct ttcggccgcc tcctcttcct
     3541 gcatccccac cgcttgaagg ccccttccct tcccagagtg atgtgcatcc cctacctgct
     3601 gagggataca ataaggtagg gccaggctat ggccctgggg agggggctcc ggagcaggag
     3661 aaatccaggg gtggctacag cagcggcttc cgagacagtg tccctatcca gggtatcacg
     3721 ctggaggaag gtgggtgtgg gactgggggc tgtgagtgtg agtgtgtgca agagattgct
     3781 ctgcatgttt gctgatctag agggcccgtt taaacccgct gatcagcctc gactgtgcct
     3841 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     3901 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     3961 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     4021 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc
     4081 tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     4141 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     4201 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     4261 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     4321 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     4381 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     4441 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     4501 gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt
     4561 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     4621 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     4681 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     4741 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     4801 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     4861 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct gatcaagaga
     4921 caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg
     4981 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
     5041 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
     5101 ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg
     5161 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
     5221 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
     5281 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
     5341 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
     5401 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
     5461 tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc
     5521 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
     5581 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
     5641 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
     5701 tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac
     5761 cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga
     5821 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
     5881 tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa
     5941 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     6001 tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta
     6061 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     6121 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     6181 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     6241 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     6301 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     6361 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     6421 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     6481 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     6541 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     6601 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     6661 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     6721 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     6781 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     6841 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     6901 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     6961 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggttt
     7021 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     7081 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     7141 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     7201 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     7261 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     7321 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     7381 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     7441 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     7501 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     7561 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     7621 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     7681 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     7741 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     7801 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     7861 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     7921 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     7981 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     8041 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     8101 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     8161 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     8221 gccacctgac gtc
//