| 质粒名称: | pHAGE-FGFR3__TACC3 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 116818 |
| 存储实验室: | Gordon Mills, Kenneth Scott |
|
载体骨架基本信息 |
|
| 载体名称: | pHAGE |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 慢病毒表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | FGFR3__TACC3 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | Gateway克隆 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | |
| 参考文献部分: | Systematic Functional Annotation of Somatic Mutations in Cancer. Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z, Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott KL, Yi S, Sahni N, Liang H, Mills GB. Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 10.1016/j.ccell.2018.01.021. 10.1016/j.ccell.2018.01.021 PubMed 29533785 |
买家导航
pHAGE-FGFR3__TACC3载体质粒基本信息
pHAGE-FGFR3__TACC3质粒图谱载体图谱和pHAGE-FGFR3__TACC3载体序列质粒序列多克隆位点信息
pHAGE-FGFR3__TACC3质粒载体简介
pHAGE-FGFR3__TACC3质粒序列载体序列
LOCUS pHAGE-FGFR3__TACC3 11381 bp ds-DNA circular SYN 12-APR-2019
DEFINITION Lentiviral expression of FGFR3__TACC3 fusion.
ACCESSION .
VERSION .
KEYWORDS pHAGE-FGFR3__TACC3
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11381)
AUTHORS Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K,
Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip
CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R,
Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott
KL, Yi S, Sahni N, Liang H, Mills GB
TITLE Systematic Functional Annotation of Somatic Mutations in Cancer.
JOURNAL Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi:
10.1016/j.ccell.2018.01.021.
PUBMED 29533785
REFERENCE 2 (bases 1 to 11381)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 12, 2019 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..11381
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 1..634
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 681..806
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1303..1536
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 2058..2175
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 2223..3401
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
intron 2454..3392
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of human
EF-1-alpha"
primer_bind 3349..3369
/label=EF1a-F
/note="Human elongation factor-1a promoter, forward primer"
protein_bind 3421..3445
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
CDS 6292..6315
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
/translation="DYKDDDDK"
protein_bind complement(6318..6342)
/gene="mutant version of attB"
/label=attB2
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
CDS 6395..6436
/codon_start=1
/product="epitope tag from simian virus 5"
/label=V5 tag
/translation="GKPIPNPLLGLDST"
misc_feature 6457..7024
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
misc_feature 6474..7026
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(6618..6635)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 6845..6864
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
CDS 7038..7757
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(7083..7104)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(7344..7363)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 7691..7712
/label=EGFP-C
/note="EGFP, forward primer"
misc_feature 7771..8359
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(7824..7844)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(8242..8253)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 8434..8667
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
primer_bind complement(8786..8804)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 8872..8976
/gene="bla"
/label=AmpR promoter
CDS 8977..9837
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(9195..9214)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 10008..10596
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 10497..10516
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 10750..10767
/label=L4440
/note="L4440 vector, forward primer"
primer_bind 10859..10878
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
protein_bind 11007..11028
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 11043..11073
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 11081..11097
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 11086..11108
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 11105..11121
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 11105..11121
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 11171..11190
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
ORIGIN
1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
3421 acaagtttgt acaaaaaagt tggcatggga gcaccagctt gtgccctggc tctgtgtgtg
3481 gccgtggcta ttgtggctgg cgcctctagc gagtctctgg gcacagagca gagagtcgtg
3541 ggcagagccg ctgaagtgcc tggacctgag cctggccagc aagagcagct ggtgtttggc
3601 tctggcgacg ccgtggaact gagctgtcct cctcctggtg gcggccctat gggccctaca
3661 gtgtgggtta aggacggcac cggactggtg cctagcgaga gagtgctcgt tggccctcag
3721 agactgcagg tcctgaacgc ctctcacgag gactccggcg cctacagctg cagacagagg
3781 ctgacccagc gggtgctgtg ccacttctcc gtcagagtga ccgatgcccc tagcagcggc
3841 gacgacgagg atggcgagga tgaggccgaa gataccggcg tggacacagg cgccccttac
3901 tggaccagac ccgagcggat ggacaagaaa ctgctggccg tgcctgccgc caacaccgtg
3961 cggtttagat gcccagccgc cggaaacccc accccttcca tcagctggct gaagaacgga
4021 agagagttcc ggggcgagca ccggatcggc ggcattaagc tgagacacca gcagtggtcc
4081 ctggtcatgg aaagcgtggt gccctccgac cggggcaact acacctgtgt ggtggaaaac
4141 aagttcggca gcatccggca gacctacacc ctggacgtgc tggaaagaag ccctcacaga
4201 cccatcctgc aggccggact gcctgccaat cagacagccg tgctgggcag cgacgtggaa
4261 tttcactgca aggtgtacag cgacgcccag ccccacatcc agtggctgaa acacgtggaa
4321 gtgaacggca gcaaagtggg ccccgacggc acaccttacg tgaccgtgct gaaaacagcc
4381 ggcgctaaca ccaccgacaa agaactggaa gtgctgtccc tgcacaacgt gaccttcgag
4441 gatgccggcg agtacacatg tctggccggc aacagcatcg gcttctccca ccattctgcc
4501 tggctggtgg tgctgcccgc cgaagaggaa ctggtggaag ccgacgaggc cggaagcgtg
4561 tacgccggaa tcctgagcta cggcgtgggc tttttcctgt tcatcctggt tgtggccgcc
4621 gtgaccctgt gcagactgag aagcccacct aagaagggcc tgggctcccc taccgtgcac
4681 aagatcagcc ggttccctct gaagagacag gtgtccctgg aaagcaacgc ctccatgagc
4741 agcaacaccc ctctcgtgcg gatcgccaga ctgtctagcg gcgaaggccc aaccctggcc
4801 aacgtgtcag agctggaact gccagccgac cccaagtggg agctgagcag agctagactg
4861 accctgggca agcctctggg cgagggctgc tttggacagg ttgtgatggc cgaggccatc
4921 ggcatcgata aggatagagc cgccaagccc gtgacagtgg cagtgaagat gctgaaggac
4981 gacgccacag acaaggacct gtccgacctg gtgtccgaga tggaaatgat gaagatgatc
5041 ggcaagcaca agaacatcat caatctgctg ggagcctgca cccagggcgg acccctgtat
5101 gtgctggtcg aatacgccgc caaaggcaac ctgcgcgagt tcctgagagc cagaaggcct
5161 cccggcctgg actacagctt cgatacctgc aagccacccg aggaacagct gacctttaag
5221 gacctcgtgt cctgcgccta tcaggtggcc agaggcatgg aatacctggc ctcccagaag
5281 tgcatccacc gggacctggc cgccagaaac gtgctcgtga ccgaggacaa cgtcatgaag
5341 atcgccgatt tcggcctggc ccgggatgtg cacaatctgg attactacaa gaaaaccacc
5401 aacggccggc tgccagtgaa gtggatggcc cctgaggccc tgttcgacag agtgtacacc
5461 caccagtctg acgtgtggtc cttcggcgtg ctgctgtggg agatcttcac actgggcggc
5521 agcccctacc ctggcatccc cgtggaagaa ctgttcaagc tgctgaaaga gggccaccgg
5581 atggataagc ccgccaattg cacccacgac ctgtatatga tcatgcggga atgctggcac
5641 gccgctccaa gccagcggcc taccttcaaa cagctggttg aggacctgga cagggtgctg
5701 accgtgacct ccaccgacgt gaaggccacc caagaggaaa accgcgagct gcggagcaga
5761 tgcgaggaac tccacggcaa gaacctggaa ctcggcaaga tcatggacag attcgaagag
5821 gtggtgtacc aggccatgga agaggtccag aagcagaaag agctgtctaa ggccgagatc
5881 cagaaagtcc tgaaagaaaa ggaccagctc accacggacc tgaacagcat ggaaaagagc
5941 ttcagcgacc tgtttaagcg cttcgaaaaa cagaaagaag tgatcgaggg ctatcggaag
6001 aacgaggaat ccctgaagaa gtgcgtcgag gactacctgg ctcggatcac acaagagggg
6061 cagcggtatc aggccctgaa ggctcacgcc gaggaaaaac tccagctggc caatgaagag
6121 attgcccaag tgcggtccaa ggcccaggct gaagcactgg cactgcaggc ttccctgcgg
6181 aaagaacaga tgcggatcca gagcctggaa aaaacagtcg agcaaaagac caaagagaac
6241 gaagaactca cccggatctg cgacgacctg atcagcaaga tggaaaaaat cgactacaag
6301 gacgatgacg acaagtgacc caactttctt gtacaaagtg gttgatatcc agcacagtgg
6361 cggccgctcg agtctagagg gcccgcggtt cgaaggtaag cctatcccta accctctcct
6421 cggtctcgat tctacgcgta ccggttagta atgagatccc tccccccccc ctaacgttac
6481 tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat
6541 attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat
6601 tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga
6661 agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca
6721 gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac
6781 acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt
6841 caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca
6901 ttgtatggga tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt
6961 aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg
7021 ataatatggc cacacatatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
7081 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
7141 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
7201 ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct
7261 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
7321 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
7381 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
7441 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
7501 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
7561 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
7621 tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga
7681 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
7741 acgagctgta caagtaaatc gatagatcct aatcaacctc tggattacaa aatttgtgaa
7801 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
7861 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
7921 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
7981 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
8041 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
8101 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
8161 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
8221 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
8281 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
8341 ctttgggccg cctccccgcc tgagatcctt taagaccaat gacttacaag gcagctgtag
8401 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
8461 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
8521 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
8581 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
8641 tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt
8701 tataacttgc aaagaaatga atatcagaga gtgagaggcc cgggttaatt aaggaaaggg
8761 ctagatcatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
8821 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
8881 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
8941 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
9001 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
9061 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
9121 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
9181 acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa
9241 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
9301 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
9361 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
9421 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
9481 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
9541 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
9601 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
9661 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
9721 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
9781 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
9841 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
9901 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
9961 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
10021 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
10081 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
10141 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
10201 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
10261 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
10321 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
10381 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
10441 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
10501 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
10561 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
10621 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
10681 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
10741 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
10801 ctccccgcgc gttggccgat tcattaatgc agcaagctca tggctgacta atttttttta
10861 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
10921 tttttggagg cctaggcttt tgcaaaaagc tccccgtggc acgacaggtt tcccgactgg
10981 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
11041 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
11101 cacacaggaa acagctatga catgattacg aatttcacaa ataaagcatt tttttcactg
11161 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaactgg
11221 ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat taccactgcc
11281 aattacctgt ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa
11341 agaaattgta tttgttaaat atgtactaca aacttagtag t
//
