| 质粒名称: | pQP-iRIS |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 102584 |
| 存储实验室: | Vladislav Verkhusha |
|
载体骨架基本信息 |
|
| 载体名称: | pIRES2-EGFP |
| 载体出品公司: | -- |
| 载体抗性: | 卡那霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | -- |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | BphP1-mVenus-CAAX-IRES2-NES-mCherry-QPAS1-AsLOV2cNLS |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pQP-iRIS was a gift from Vladislav Verkhusha (Addgene plasmid # 102584 ; http://n2t.net/addgene:102584 ; RRID:Addgene_102584) |
| 参考文献部分: | Near-infrared optogenetic pair for protein regulation and spectral multiplexing. Redchuk TA, Omelina ES, Chernov KG, Verkhusha VV. Nat Chem Biol. 2017 Mar 27. doi: 10.1038/nchembio.2343. 10.1038/nchembio.2343 PubMed 28346403 |
买家导航
pQP-iRIS载体 pQPiRIS质粒基本信息
pQP-iRIS质粒图谱 pQPiRIS载体图谱和pQP-iRIS载体序列 pQPiRIS质粒序列多克隆位点信息
pQP-iRIS质粒 pQPiRIS载体简介
pQP-iRIS质粒序列 pQPiRIS载体序列
LOCUS pQP-iRIS 9403 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Regulation of protein localization
(near-infrared-blue-light-inducible shuttle).
ACCESSION .
VERSION .
KEYWORDS pQP-iRIS
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9403)
AUTHORS Redchuk TA, Omelina ES, Chernov KG, Verkhusha VV
TITLE Near-infrared optogenetic pair for protein regulation and spectral
multiplexing.
JOURNAL Nat Chem Biol. 2017 Mar 27. doi: 10.1038/nchembio.2343.
PUBMED 28346403
REFERENCE 2 (bases 1 to 9403)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9403
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 61..364
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 365..568
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 519..539
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
regulatory 616..625
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 2859..3569
/codon_start=1
/product="Venus YFP with monomerizing A206K mutation (Nagai
et al., 2002)"
/label=mVenus
/note="mammalian codon-optimized"
/translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKL
ICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKAN
FKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLER
VTAAGITLGMDELYK"
primer_bind complement(2898..2919)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(3159..3178)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
misc_feature 3624..3680
/label=MCS
/note="multiple cloning site"
misc_feature 3682..4256
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
misc_feature 3706..4258
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(3850..3867)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 4077..4096
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
CDS 4315..5022
/codon_start=1
/product="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
/label=mCherry
/note="mammalian codon-optimized"
/translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
EGRHSTGGMDELYK"
primer_bind complement(4460..4478)
/label=mCherry-R
/note="mCherry, reverse primer"
primer_bind 4728..4747
/label=mCherry-F
/note="mCherry, forward primer"
CDS 5527..5541
/codon_start=1
/product="enterokinase recognition and cleavage site"
/label=enterokinase site
/translation="DDDDK"
polyA_signal 6191..6312
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(6228..6247)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 6282..6301
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(6319..6774)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(6456..6477)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 6668..6687
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 6801..6905
/gene="bla"
/label=AmpR promoter
promoter 6907..7264
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
primer_bind complement(6930..6950)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 7115..7250
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 7177..7196
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 7299..8093
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(7353..7372)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 7963..7982
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(8281..8300)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 8325..8372
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 8701..9289
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 9190..9209
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
601 ccggactcag atctaccatg gtggcaggtc atgcctctgg cagccccgca ttcgggaccg
661 ccgatctttc gaattgcgaa cgtgaagaga tccacctcgc cggctcgatc cagccgcatg
721 gcgcgcttct ggtcgtcagc gagccggatc atcgcatcat ccaggccagc gccaacgccg
781 cggaatttct gaatctcgga agcgtgctcg gcgttccgct cgccgagatc gacggcgatc
841 tgttgatcaa gatcctgccg catctcgatc ccaccgccga aggcatgccg gtcgcggtgc
901 gctgccggat cggcaatccc tccacggagt acgacggtct gatgcatcgg cctccggaag
961 gcgggctgat catcgagctc gaacgtgccg gcccgccgat cgatctgtcc ggcacgctgg
1021 cgccggcgct ggagcggatc cgcacggcgg gctcgctgcg cgcgctgtgc gatgacaccg
1081 cgctgctgtt tcagcagtgc accggctacg accgggtgat ggtgtatcgc ttcgacgagc
1141 agggccacgg cgaagtgttc tccgagcgcc acgtgcccgg gctcgaatcc tatttcggca
1201 accgctatcc gtcgtcggac attccgcaga tggcgcggcg gctgtacgag cggcagcgcg
1261 tccgcgtgct ggtcgacgtc agctatcagc cggtgccgct ggagccgcgg ctgtcgccgc
1321 tgaccgggcg cgatctcgac atgtcgggct gcttcctgcg ctcgatgtcg ccgatccatc
1381 tgcagtacct gaagaacatg ggcgtgcgcg ccaccctggt ggtgtcgctg gtggtcggcg
1441 gcaagctgtg gggcctggtt gcctgtcacc attatctgcc gcgcttcatc catttcgagc
1501 tgcgggcgat ctgcgaactg ctcgccgaag cgatcgcgac gcggatcacc gcgcttgaga
1561 gcttcgcgca gagccagtcg gagctgttcg tgcagcggct cgaacagcgc atgatcgaag
1621 cgatcacccg tgaaggcgat tggcgcgcag cgattttcga caccagccaa tcgatcctgc
1681 agccgctgca cgccgacggt tgcgcgctgg tgtacgaaga ccagatcagg accatcggtg
1741 acgtaccttc cacgcaggat gttcgcgaga tcgccgggtg gctcgatcgc cagccacgtg
1801 cggcggtgac ctcgaccgcg tcgctcggtc tcgacgtgcc ggagctcgcg catctgacgc
1861 ggatggcgag cggcgtggtc gcggcgccga tttcggatca tcgcggcgag tttctgatgt
1921 ggttccgccc cgagcgcgtc cacaccgtta cctggggcgg cgatccgaag aagccgttca
1981 cgatgggcga tacaccggcg gatctgtcgc cgcggcgctc cttcgccaaa tggcatcagg
2041 ttgtcgaagg cacgtccgat ccgtggacgg ccgccgatct cgccgcggct cgcaccatcg
2101 gtcagaccgt cgccgacatc gtgctgcaat tccgcgcggt gcggacactg atcgcccgcg
2161 aacagtacga acagttttcg tcccaggtgc acgcttcgat gcagccggtg ctgatcaccg
2221 acgccgaagg ccgcatcctg ctgatgaacg actcgttccg cgacatgttg ccggcggggt
2281 cgccatccgc cgtccatctc gacgatctcg ccgggttctt cgtcgaatcg aacgatttcc
2341 tgcgcaacgt cgccgaactg atcgatcacg gccgcgggtg gcgcggcgaa gttctgctgc
2401 gcggcgcagg taatcgcccg ttgccgctgg cagtgcgcgc cgatccggtg acgcgcacgg
2461 aggaccagtc gctcggcttc gtgctgatct tcagcgacgc taccgatcgt cgcaccgcag
2521 atgccgcacg cacgcgtttc caggaaggca ttcttgccag cgcacgtccc ggcgtgcggc
2581 tcgactccaa gtccgacctc ttgcacgaga agctgctgtc cgcgctggtc gagaacgcgc
2641 agcttgccgc attggaaatt acttacggcg tcgagaccgg acgcatcgcc gagctgctcg
2701 aaggcgttcg ccagtcgatg ctgcgcaccg ccgaagtgct cggccatctg gtgcagcacg
2761 cggcgcgcac ggccggcagc gacagctcga gcaatggctc gcagaacaag aaggctagca
2821 gtgctggcgg tagtgctggt agtgctggtg gcactagtag caagggcgag gagctgttca
2881 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
2941 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ctgatctgca
3001 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgggc tacggcctgc
3061 agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
3121 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
3181 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
3241 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
3301 acgtctatat caccgccgac aagcagaaga acggcatcaa ggccaacttc aagatccgcc
3361 acaacatcga ggacggcggc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
3421 gcgacggccc cgtgctgctg cccgacaacc actacctaag ctaccagtcc aagctgagca
3481 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga acgcgtgacc gccgccggga
3541 tcactctcgg catggacgag ctgtacaagg ctagcggtaa aaagaagaaa aagaagtcaa
3601 agacaaagtg tgtaattatg taaagatctc gagctcaagc ttcgaattct gcagtcgacg
3661 gtaccgcggg cccgggatcc gcccctctcc ctcccccccc ccctaacgtt actggccgaa
3721 gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt
3781 cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg
3841 gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc
3901 ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc
3961 ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa
4021 aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc
4081 tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg
4141 gatctgatct ggggcctcgg tacacatgct ttacatgtgt ttagtcgagg ttaaaaaaac
4201 gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataatatg
4261 gccacaaacc ggtccatggg aattgatctc tcaggattaa cactgcagac tagtatggtg
4321 agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac
4381 atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc
4441 tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc
4501 tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc
4561 gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg
4621 aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag
4681 ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag
4741 aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg
4801 aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc
4861 aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc
4921 aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc
4981 gagggccgcc actccaccgg cggcatggac gagctgtaca agagtgctgg tgggtccgcc
5041 ggcgggagtg ctggcggtag tgccggcggt agttctagag gcaagaacat gcaggcggtc
5101 accgagctgc attcccggct gatcgctgcg cagcaggcga tggagcgcga ctattggcgg
5161 ttgcgtgaat tggagactcg ctaccgcctg gtgttcgacg ctgccgccga tgcggtgatg
5221 atcgtctccg ccggcgacat gcgcatcgtc gaagccaacc gggcggcggt gaatgcgatc
5281 agccgcgtcg agcgcggcaa tgacgacctt gcggggcgtg atttcctcgc cgaagtggcg
5341 gctgccgatc gcgatgcggt gcgcgacatg ctggcccagg tgcgtcagcg cggcaccgca
5401 ctcagcgtcc tcgttcatct cggccgttac gaccgcgcct ggatgctgcg cggttcgctg
5461 atgtcgtccg agcgtcgtca ggttttcctg ctgcacttca ccccggtgac cacgactccc
5521 gcgatcgacg acgacgacaa aggtgtcgtt gcttctgctg ccgacggtgc agagggcgcc
5581 tcagacgacg cagaggatga attcccggct agcggcgccg gcgccttttt ggctactaca
5641 cttgaacgta ttgagaagaa ctttgtcatt actgacccaa ggttgccaga taatcccatt
5701 atattcgcgt ccgatagttt cttgcagttg acagaatata gccgtgaaga aattttggga
5761 agaaactgca ggtttctaca aggtcctgaa actgatcgcg cgacagtgag aaaaattaga
5821 gatgccatag ataaccaaac agaggtcact gttcagctga ttaattatac aaagagtggt
5881 aaaaagttct ggaacctctt tcacttgcag cctatgcgag atcagaaggg agatgtccag
5941 tactttattg gggttcagtt ggatggaact gagcatgtcc gagatgctgc cgagagagag
6001 ggcgttatgc tggccaagaa gaccgccgag aacatcgaca tggctgccaa gagggtcaag
6061 ttggactgag cggccgcgac tctagatcat aatcagccat accacatttg tagaggtttt
6121 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
6181 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
6241 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
6301 caatgtatct taaggcgtaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt
6361 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
6421 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
6481 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
6541 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc
6601 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
6661 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
6721 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg
6781 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
6841 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
6901 agagtcctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc
6961 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
7021 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
7081 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
7141 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
7201 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
7261 caaagatcga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc
7321 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
7381 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
7441 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca gcgcggctat
7501 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
7561 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
7621 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
7681 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
7741 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
7801 ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc
7861 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
7921 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
7981 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
8041 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac
8101 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc
8161 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
8221 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccctaggg ggaggctaac
8281 tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa taaaaagaca
8341 gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg
8401 gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg tttcttcctt
8461 ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca acgtcggggc
8521 ggcaggccct gccatagcct caggttactc atatatactt tagattgatt taaaacttca
8581 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
8641 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
8701 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
8761 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
8821 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
8881 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
8941 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
9001 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
9061 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
9121 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
9181 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
9241 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
9301 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
9361 gttatcccct gattctgtgg ataaccgtat taccgccatg cat
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