px330-U6-SpCas9 gRNA(Bbs1)-CAG-SpCas9-P2A-mCherry

LOCUS      px330-U6-SpCas9 gRNA(Bbs1)-CAG-SpCas9-P2A-mCherry               9281 bp DNA     circular SYN 20-APR-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9281)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Aug 26, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..9281
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        51..70
                     /label=chicken beta-actin promoter
                     /note="contains a 21-bp deletion relative to the standard 
                     chicken beta-actin promoter"
     intron          207..434
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     regulatory      446..455
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             455..520
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             527..547
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             572..4672
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4673..4720
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4730..4786
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4787..5497
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     DDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     misc_feature    4803..4808
                     /label=SDS
     misc_feature    5235..5240
                     /label=SDS
     polyA_signal    5528..5735
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   5744..5884
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    5744..5873
                     /label=AAV2 ITR
     rep_origin      5959..6414
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        6696..6800
                     /gene="bla"
                     /label=AmpR promoter
     CDS             6801..7661
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7832..8420
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        8482..8730
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        8752..8827
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        8924..9209
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     promoter        join(9211..9281,1..206)
                     /label=chicken beta-actin promoter
                     /note="contains a 21-bp deletion relative to the standard 
                     chicken beta-actin promoter"
ORIGIN
        1 tttatttttt aattattttg tgcagcgatg ggggcggggg gggggggggg cgcgcgccag
       61 gcggggcggg gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg gcggcagcca
      121 atcagagcgg cgcgctccga aagtttcctt ttatggcgag gcggcggcgg cggcggccct
      181 ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc cccgtgcccc
      241 gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta ctcccacagg
      301 tgagcgggcg ggacggccct tctcctccgg gctgtaatta gctgagcaag aggtaagggt
      361 ttaagggatg gttggttggt ggggtattaa tgtttaatta cctggagcac ctgcctgaaa
      421 tcactttttt tcaggttgga ccggtgccac catggactat aaggaccacg acggagacta
      481 caaggatcat gatattgatt acaaagacga tgacgataag atggccccaa agaagaagcg
      541 gaaggtcggt atccacggag tcccagcagc cgacaagaag tacagcatcg gcctggacat
      601 cggcaccaac tctgtgggct gggccgtgat caccgacgag tacaaggtgc ccagcaagaa
      661 attcaaggtg ctgggcaaca ccgaccggca cagcatcaag aagaacctga tcggagccct
      721 gctgttcgac agcggcgaaa cagccgaggc cacccggctg aagagaaccg ccagaagaag
      781 atacaccaga cggaagaacc ggatctgcta tctgcaagag atcttcagca acgagatggc
      841 caaggtggac gacagcttct tccacagact ggaagagtcc ttcctggtgg aagaggataa
      901 gaagcacgag cggcacccca tcttcggcaa catcgtggac gaggtggcct accacgagaa
      961 gtaccccacc atctaccacc tgagaaagaa actggtggac agcaccgaca aggccgacct
     1021 gcggctgatc tatctggccc tggcccacat gatcaagttc cggggccact tcctgatcga
     1081 gggcgacctg aaccccgaca acagcgacgt ggacaagctg ttcatccagc tggtgcagac
     1141 ctacaaccag ctgttcgagg aaaaccccat caacgccagc ggcgtggacg ccaaggccat
     1201 cctgtctgcc agactgagca agagcagacg gctggaaaat ctgatcgccc agctgcccgg
     1261 cgagaagaag aatggcctgt tcggaaacct gattgccctg agcctgggcc tgacccccaa
     1321 cttcaagagc aacttcgacc tggccgagga tgccaaactg cagctgagca aggacaccta
     1381 cgacgacgac ctggacaacc tgctggccca gatcggcgac cagtacgccg acctgtttct
     1441 ggccgccaag aacctgtccg acgccatcct gctgagcgac atcctgagag tgaacaccga
     1501 gatcaccaag gcccccctga gcgcctctat gatcaagaga tacgacgagc accaccagga
     1561 cctgaccctg ctgaaagctc tcgtgcggca gcagctgcct gagaagtaca aagagatttt
     1621 cttcgaccag agcaagaacg gctacgccgg ctacattgac ggcggagcca gccaggaaga
     1681 gttctacaag ttcatcaagc ccatcctgga aaagatggac ggcaccgagg aactgctcgt
     1741 gaagctgaac agagaggacc tgctgcggaa gcagcggacc ttcgacaacg gcagcatccc
     1801 ccaccagatc cacctgggag agctgcacgc cattctgcgg cggcaggaag atttttaccc
     1861 attcctgaag gacaaccggg aaaagatcga gaagatcctg accttccgca tcccctacta
     1921 cgtgggccct ctggccaggg gaaacagcag attcgcctgg atgaccagaa agagcgagga
     1981 aaccatcacc ccctggaact tcgaggaagt ggtggacaag ggcgcttccg cccagagctt
     2041 catcgagcgg atgaccaact tcgataagaa cctgcccaac gagaaggtgc tgcccaagca
     2101 cagcctgctg tacgagtact tcaccgtgta taacgagctg accaaagtga aatacgtgac
     2161 cgagggaatg agaaagcccg ccttcctgag cggcgagcag aaaaaggcca tcgtggacct
     2221 gctgttcaag accaaccgga aagtgaccgt gaagcagctg aaagaggact acttcaagaa
     2281 aatcgagtgc ttcgactccg tggaaatctc cggcgtggaa gatcggttca acgcctccct
     2341 gggcacatac cacgatctgc tgaaaattat caaggacaag gacttcctgg acaatgagga
     2401 aaacgaggac attctggaag atatcgtgct gaccctgaca ctgtttgagg acagagagat
     2461 gatcgaggaa cggctgaaaa cctatgccca cctgttcgac gacaaagtga tgaagcagct
     2521 gaagcggcgg agatacaccg gctggggcag gctgagccgg aagctgatca acggcatccg
     2581 ggacaagcag tccggcaaga caatcctgga tttcctgaag tccgacggct tcgccaacag
     2641 aaacttcatg cagctgatcc acgacgacag cctgaccttt aaagaggaca tccagaaagc
     2701 ccaggtgtcc ggccagggcg atagcctgca cgagcacatt gccaatctgg ccggcagccc
     2761 cgccattaag aagggcatcc tgcagacagt gaaggtggtg gacgagctcg tgaaagtgat
     2821 gggccggcac aagcccgaga acatcgtgat cgaaatggcc agagagaacc agaccaccca
     2881 gaagggacag aagaacagcc gcgagagaat gaagcggatc gaagagggca tcaaagagct
     2941 gggcagccag atcctgaaag aacaccccgt ggaaaacacc cagctgcaga acgagaagct
     3001 gtacctgtac tacctgcaga atgggcggga tatgtacgtg gaccaggaac tggacatcaa
     3061 ccggctgtcc gactacgatg tggaccatat cgtgcctcag agctttctga aggacgactc
     3121 catcgacaac aaggtgctga ccagaagcga caagaaccgg ggcaagagcg acaacgtgcc
     3181 ctccgaagag gtcgtgaaga agatgaagaa ctactggcgg cagctgctga acgccaagct
     3241 gattacccag agaaagttcg acaatctgac caaggccgag agaggcggcc tgagcgaact
     3301 ggataaggcc ggcttcatca agagacagct ggtggaaacc cggcagatca caaagcacgt
     3361 ggcacagatc ctggactccc ggatgaacac taagtacgac gagaatgaca agctgatccg
     3421 ggaagtgaaa gtgatcaccc tgaagtccaa gctggtgtcc gatttccgga aggatttcca
     3481 gttttacaaa gtgcgcgaga tcaacaacta ccaccacgcc cacgacgcct acctgaacgc
     3541 cgtcgtggga accgccctga tcaaaaagta ccctaagctg gaaagcgagt tcgtgtacgg
     3601 cgactacaag gtgtacgacg tgcggaagat gatcgccaag agcgagcagg aaatcggcaa
     3661 ggctaccgcc aagtacttct tctacagcaa catcatgaac tttttcaaga ccgagattac
     3721 cctggccaac ggcgagatcc ggaagcggcc tctgatcgag acaaacggcg aaaccgggga
     3781 gatcgtgtgg gataagggcc gggattttgc caccgtgcgg aaagtgctga gcatgcccca
     3841 agtgaatatc gtgaaaaaga ccgaggtgca gacaggcggc ttcagcaaag agtctatcct
     3901 gcccaagagg aacagcgata agctgatcgc cagaaagaag gactgggacc ctaagaagta
     3961 cggcggcttc gacagcccca ccgtggccta ttctgtgctg gtggtggcca aagtggaaaa
     4021 gggcaagtcc aagaaactga agagtgtgaa agagctgctg gggatcacca tcatggaaag
     4081 aagcagcttc gagaagaatc ccatcgactt tctggaagcc aagggctaca aagaagtgaa
     4141 aaaggacctg atcatcaagc tgcctaagta ctccctgttc gagctggaaa acggccggaa
     4201 gagaatgctg gcctctgccg gcgaactgca gaagggaaac gaactggccc tgccctccaa
     4261 atatgtgaac ttcctgtacc tggccagcca ctatgagaag ctgaagggct cccccgagga
     4321 taatgagcag aaacagctgt ttgtggaaca gcacaagcac tacctggacg agatcatcga
     4381 gcagatcagc gagttctcca agagagtgat cctggccgac gctaatctgg acaaagtgct
     4441 gtccgcctac aacaagcacc gggataagcc catcagagag caggccgaga atatcatcca
     4501 cctgtttacc ctgaccaatc tgggagcccc tgccgccttc aagtactttg acaccaccat
     4561 cgaccggaag aggtacacca gcaccaaaga ggtgctggac gccaccctga tccaccagag
     4621 catcaccggc ctgtacgaga cacggatcga cctgtctcag ctgggaggcg acaaaaggcc
     4681 ggcggccacg aaaaaggccg gccaggcaaa aaagaaaaag ggaagcggag ctactaactt
     4741 cagcctgctg aagcaggctg gagacgtgga ggagaaccct ggacctatgg tgagcaaggg
     4801 cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg
     4861 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg
     4921 cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat
     4981 cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc
     5041 cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga
     5101 tgacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta
     5161 caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaaaac
     5221 catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga
     5281 gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac
     5341 ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga
     5401 catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg
     5461 ccactccacc ggcggcatgg acgagctgta caagtaagaa ttcctagagc tcgctgatca
     5521 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     5581 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     5641 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     5701 gaggattggg aagagaatag caggcatgct ggggagcggc cgcaggaacc cctagtgatg
     5761 gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc
     5821 gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg
     5881 caggggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
     5941 cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     6001 ttacgcgcag cgtgaccgct acacttgcca gcgccttagc gcccgctcct ttcgctttct
     6061 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     6121 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg
     6181 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     6241 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac tctatctcgg
     6301 gctattcttt tgatttataa gggattttgc cgatttcggt ctattggtta aaaaatgagc
     6361 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca attttatggt
     6421 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
     6481 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     6541 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     6601 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     6661 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     6721 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     6781 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     6841 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     6901 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     6961 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     7021 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     7081 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     7141 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     7201 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     7261 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     7321 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     7381 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     7441 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     7501 gagccggtga gcgtggaagc cgcggtatca ttgcagcact ggggccagat ggtaagccct
     7561 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     7621 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     7681 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     7741 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     7801 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     7861 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     7921 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     7981 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     8041 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     8101 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     8161 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     8221 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     8281 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     8341 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     8401 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     8461 gctggccttt tgctcacatg tgagggccta tttcccatga ttccttcata tttgcatata
     8521 cgatacaagg ctgttagaga gataattgga attaatttga ctgtaaacac aaagatatta
     8581 gtacaaaata cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta
     8641 tgttttaaaa tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct
     8701 ttatatatct tgtggaaagg acgaaacacc gtatggtaag acatgcagac cgttttagag
     8761 ctagaaatag caagttaaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag
     8821 tcggtgcttt tttgttttag agctagaaat agcaagttaa aataaggcta gtccgttttt
     8881 agcgcgtgcg ccaattctgc agacaaatgg ctctagaggt acccgttaca taacttacgg
     8941 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca atagtaacgc
     9001 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
     9061 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
     9121 ggcccgcctg gcattgtgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     9181 tctacgtatt agtcatcgct attaccatgg tcgaggtgag ccccacgttc tgcttcactc
     9241 tccccatctc ccccccctcc ccacccccaa ttttgtattt a
//