| 质粒名称: | pcDNA3.1-Peredox-mCherry-NLS |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 32384 |
| 存储实验室: | Gary Yellen |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.1 |
| 载体出品公司: | InVitrogen |
| 空载体大小: | 5400bp |
| 完整质粒大小: | 8155bp |
| 载体类型: | 哺乳细胞表达 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | Peredox-mCherry-NLS |
| 插入大小: | 2800bp |
| 启动子: | CMV |
| 标签/融合蛋白: | Nuclear Localization Seq (C terminal on insert) |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | XbaI (not destroyed) |
| 3’克隆位点: | XhoI (destroyed during cloning) |
| 5’测序引物 | T7 |
| 3’测序引物 | mCherry-R |
|
文献引用方法 |
|
| 材料和方法部分: | pcDNA3.1-Peredox-mCherry-NLS was a gift from Gary Yellen (Addgene plasmid # 32384 ; http://n2t.net/addgene:32384 ; RRID:Addgene_32384) |
| 参考文献部分: | Imaging Cytosolic NADH-NAD(+) Redox State with a Genetically Encoded Fluorescent Biosensor. Hung YP, Albeck JG, Tantama M, Yellen G. Cell Metab. 2011 Oct 5;14(4):545-54. 10.1016/j.cmet.2011.08.012 PubMed 21982714 |
买家导航
pcDNA3.1-Peredox-mCherry-NLS载体质粒基本信息
pcDNA3.1-Peredox-mCherry-NLS质粒图谱载体图谱和pcDNA3.1-Peredox-mCherry-NLS载体序列质粒序列多克隆位点信息
pcDNA3.1-Peredox-mCherry-NLS质粒载体简介
pcDNA3.1-Peredox-mCherry-NLS质粒序列载体序列
LOCUS pcDNA3.1-Peredox-mCherry-NLS 8158 bp DNA circular SYN 14-APR-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8158)
AUTHORS Hung YP, Albeck JG, Tantama M, Yellen G
TITLE Imaging Cytosolic NADH-NAD(+) Redox State with a Genetically Encoded
Fluorescent Biosensor.
JOURNAL Cell Metab. 2011 Oct 5;14(4):545-54.
PUBMED 21982714
REFERENCE 2 (bases 1 to 8158)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Cell
Metab."; date: "2011-10-5"; volume: "14(4)"; pages: "545-54"
COMMENT Sequence Label: pcDNA3.1-Peredox-mCherry-NLS
FEATURES Location/Qualifiers
source 1..8158
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 66..85
/label=T7
/note="T7 promoter, forward primer"
promoter 66..84
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS join(133..744,745..765,766..1500,1501..1503,1504..2133,
2134..2178)
/codon_start=1
/product="genetically encoded fluorescent biosensor of the
cytosolic NADH-NAD+ redox state (Hung et al., 2011)"
/label=Peredox
/note="consists of two pH-insensitive mutant copies of the
Thermus aquaticus redox-sensing repressor Rex (T-Rex)
separated by circularly permuted monomeric T-Sapphire
(cpmTS), a pH-resistant GFP variant"
/translation="KVPEAAISRLITYLRILEELEAQGVHRTASEQLGELAQVTAFQVD
KDLSYFGSYGTDGVGYTVPVLKRELRHILGLNRKWGLCIVGMGRLGSALADWPGFGESF
ELRGFFDVDPGMVGRPVRGGVIEHVDLLPQRVPGRIEIALLTVPREAAQKAADLLVAAG
IKGILNFAPVVLEVPKEVAVENVDILAGLTRLSFAILNPTWSAAGGHGFTAHNVYIMAD
KQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSIQSKLSKDPNEK
RDHMVLLEFVTAAGITHGMDELYKGGTGGSMVSKGEELFTGVVPILVELDGDVNGHKFS
VSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFSYGVMVFARYPDHMKQHDFFKSA
MPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNTK
VPEAAISRLITYLRILEELEAQGVHRTASEQLGELAQVTAFQVDEDLSYFGSYGTDGVG
YTVPVLKRELRHILGLNRKWGLCIVGMGRLGSALADWPGFGESFELRGFFDVDPGMVGR
PVRGGVIEHVDLLPQRVPGRIEIALLTVPREAAQKAADLLVAAGIKGILNFAPVVLEVP
KEVAVENVDFLAGLTRLSFAILNPKWREEMMGSGTGGNASDGGGSGG"
CDS 2179..2886
/codon_start=1
/product="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
/label=mCherry
/note="mammalian codon-optimized"
/translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
EGRHSTGGMDELYK"
primer_bind complement(2324..2342)
/label=mCherry-R
/note="mCherry, reverse primer"
primer_bind 2592..2611
/label=mCherry-F
/note="mCherry, forward primer"
CDS 2887..2907
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
primer_bind complement(2952..2969)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 2958..3182
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 3228..3656
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(3315..3334)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 3525..3546
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(3665..3685)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 3670..3999
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 3850..3985
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 3912..3931
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 4066..4860
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(4120..4139)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 4730..4749
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 5034..5155
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(5071..5090)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 5125..5144
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(5204..5220)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5204..5220)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(5217..5239)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 5228..5244
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(join(5252..5258,5259..5276,5277..5282))
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5297..5318
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(5435..5452)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(5606..6194)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(5686..5705)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(join(6365..7156,7157..7225))
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 6988..7007
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(7226..7330)
/gene="bla"
/label=AmpR promoter
primer_bind complement(7405..7424)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 7596..7975
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter join(7976..8158,1..21)
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 8130..8150
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
ORIGIN
1 gaggtctata taagcagagc tctctggcta actagagaac ccactgctta ctggcttatc
61 gaaattaata cgactcacta tagggagacc caagctggct agctctagat tgggcccacc
121 atggttgcta gcaaagttcc tgaagcagcc atttccagac tgattactta cctgagaatt
181 ctggaagagc tggaagccca aggtgtacac cgcaccgcct ccgagcaact gggcgagctg
241 gcccaggtca ccgccttcca ggttgataag gacctgtcct acttcggctc ctacggcact
301 gacggcgttg gctacactgt accggtcctc aagagagaac tgcgccatat cctcggtctg
361 aaccgcaaat ggggcctgtg catcgtgggg atgggccgcc tgggatccgc tcttgctgat
421 tggcctggtt ttggcgagag cttcgagctg cgcggtttct tcgatgttga cccaggtatg
481 gttggtcggc cggttcgcgg tggtgttatc gaacacgttg atctgctgcc ccagcgcgta
541 cctggtcgca tcgaaatcgc tctgctgacg gttccacgcg aggcagcaca gaaggctgcc
601 gacctgctgg ttgccgctgg catcaagggc atcctgaact tcgctccagt tgtactggag
661 gttcccaagg aagtggctgt tgagaacgtt gacatcctgg ccggtctgac gcgtctgagc
721 tttgccattc tgaaccccac gtggagcgca gcaggtgggc atggtttcac tgctcacaac
781 gtgtatatca tggctgacaa acagaagaac ggtatcaagg ccaacttcaa gatccgccac
841 aacatcgaag acggcggcgt tcagctggct gatcactacc agcagaacac cccaatcggc
901 gacggccctg ttctgctgcc tgacaaccac tacttaagca tccagtctaa actgtccaaa
961 gacccaaacg agaagcgcga ccacatggtt ctgctggaat ttgttaccgc cgctggcatc
1021 acccacggca tggacgaact gtacaagggc ggcaccgggg gatctatggt ttccaaaggc
1081 gaggaactgt tcactggtgt tgttcctatc ctggttgaac tggacggcga cgttaacggc
1141 cacaaattca gcgttagcgg ggaaggcgaa ggagacgcta cctacggcaa gctgactctg
1201 aagttcatct gcaccactgg caagctgcct gttccttggc ctaccctggt tactaccttc
1261 tcctatggtg ttatggtttt cgccagatat cctgaccaca tgaagcagca cgacttcttc
1321 aagagcgcca tgcctgaggg ctacgttcag gaacgcacca tctttttcaa ggacgacggc
1381 aactataaga cacgcgctga ggttaagttc gagggcgaca ctctggttaa ccgcatcgag
1441 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg gccataagct tgaatataac
1501 accaaggtac ctgaagccgc catcagccgc ctcatcactt acctccgcat cctggaggag
1561 ctcgaagccc aaggcgttca ccgcactgca agcgagcagc tgggcgaact ggcacaggtt
1621 acagcattcc aggttgacga agatctgtcc tatttcggtt cctacggcac cgacggtgtt
1681 ggctacacag tccctgtcct gaagcgcgag ctgcgccaca tcctgggcct gaaccgcaag
1741 tggggcttat gcatcgttgg catgggccgc ctcggtagtg cactggcaga ctggccgggc
1801 tttggtgaat ctttcgaact gcgcggcttc ttcgacgttg atccaggcat ggttgggcgc
1861 cctgttcgcg gtggcgttat cgagcatgtt gacctcctgc cacagcgcgt cccgggtcgc
1921 atcgagatcg ccctgctgac cgttcctcgc gaagctgcac agaaggcagc cgacttacta
1981 gtcgccgctg gtatcaaggg aattctgaac ttcgcccctg ttgttctgga ggttcctaag
2041 gaagttgctg ttgaaaacgt tgatttcctg gccggcctga cccgcctgag cttcgcaatc
2101 ctgaatccta agtggcgcga agaaatgatg ggttccggaa ctgggggcaa cgcctctgac
2161 ggtggtgggt ctggtggtat ggtgagcaag ggcgaggagg ataacatggc catcatcaag
2221 gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc
2281 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc
2341 aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc
2401 aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag
2461 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag
2521 gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc
2581 ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg
2641 atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac
2701 ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg
2761 cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc
2821 atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgaactg
2881 tacaagccta aaaagaaacg taaagttgaa gatgcttgag tcgagaagct taagtttaaa
2941 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
3001 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
3061 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
3121 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
3181 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
3241 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
3301 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
3361 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
3421 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
3481 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
3541 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
3601 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt
3661 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
3721 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
3781 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
3841 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
3901 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
3961 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata
4021 tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat
4081 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
4141 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
4201 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg
4261 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
4321 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
4381 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
4441 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
4501 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
4561 gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc
4621 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
4681 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
4741 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
4801 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
4861 gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt
4921 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
4981 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt
5041 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
5101 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
5161 tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg
5221 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
5281 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
5341 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
5401 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
5461 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
5521 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
5581 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
5641 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
5701 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
5761 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
5821 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
5881 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
5941 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
6001 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
6061 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
6121 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
6181 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
6241 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
6301 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
6361 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
6421 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
6481 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
6541 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
6601 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
6661 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
6721 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
6781 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
6841 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
6901 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
6961 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
7021 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
7081 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
7141 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
7201 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
7261 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
7321 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc
7381 cgatccccta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta
7441 tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac
7501 aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc
7561 gctgcttcgc gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta
7621 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
7681 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
7741 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
7801 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
7861 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
7921 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat
7981 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag
8041 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
8101 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtgg
//
