| 质粒名称: | pOBCol3.6-GFPtpz |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 110211 |
| 存储实验室: | David Rowe |
|
载体骨架基本信息 |
|
| 载体名称: | pBC SK+ |
| 载体出品公司: | Stratagene |
| 载体抗性: | 氯霉素 |
| 空载体大小: | 3400 bp |
| 完整质粒大小: | 9600 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | eGFPtopaz,Topaz,YFP |
| 物种来源: | A. victoria |
| 插入大小: | 720 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | 3.6 kb rat type 1 collagen + first intron |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | Sac2(未破坏) |
| 3' 克隆位点: | Sal1 (未破坏) |
| 5' 测序引物 | T3 |
| 3' 测序引物 | T7 |
|
文献引用方法 |
|
| 材料和方法部分: | pOBCol3.6-GFPtpz was a gift from David Rowe (Addgene plasmid # 110211 ; http://n2t.net/addgene:110211 ; RRID:Addgene_110211) |
| 参考文献部分: | Use of type I collagen green fluorescent protein transgenes to identify subpopulations of cells at different stages of the osteoblast lineage. Kalajzic I, Kalajzic Z, Kaliterna M, Gronowicz G, Clark SH, Lichtler AC, Rowe D. J Bone Miner Res. 2002 Jan;17(1):15-25. doi: 10.1359/jbmr.2002.17.1.15. 10.1359/jbmr.2002.17.1.15 PubMed 11771662 |
买家导航
pOBCol3.6-GFPtpz载体 pOBCol3.6GFPtpz质粒基本信息
pOBCol3.6-GFPtpz质粒图谱 pOBCol3.6GFPtpz载体图谱和pOBCol3.6-GFPtpz载体序列 pOBCol3.6GFPtpz质粒序列多克隆位点信息
pOBCol3.6-GFPtpz质粒 pOBCol3.6GFPtpz载体简介
pOBCol3.6-GFPtpz质粒序列 pOBCol3.6GFPtpz载体序列
LOCUS pOBCol3.6-GFPtpz 9671 bp ds-DNA circular SYN 07-AUG-2018
DEFINITION Labels immature osteoblasts and connective tissue cells expressing
type 1 collagen..
ACCESSION .
VERSION .
KEYWORDS pOBCol3.6-GFPtpz
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9671)
AUTHORS Kalajzic I, Kalajzic Z, Kaliterna M, Gronowicz G, Clark SH, Lichtler
AC, Rowe D
TITLE Use of type I collagen green fluorescent protein transgenes to
identify subpopulations of cells at different stages of the
osteoblast lineage.
JOURNAL J Bone Miner Res. 2002 Jan;17(1):15-25. doi:
10.1359/jbmr.2002.17.1.15.
PUBMED 11771662
REFERENCE 2 (bases 1 to 9671)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 7, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9671
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 480..499
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 733..750
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 867..888
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 903..933
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 941..957
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 946..968
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 965..981
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 965..981
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 1000..1020
/label=T3
/note="T3 promoter, forward primer"
promoter 1002..1020
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind 6294..6310
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 6294..6310
/label=pBluescriptSK
/note="For pBluescript vector"
regulatory 6377..6386
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 6383..7102
/codon_start=1
/product="enhanced yellow variant of GFP"
/label=Topaz YFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMRQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(6428..6449)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(6689..6708)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 7036..7057
/label=EGFP-C
/note="EGFP, forward primer"
polyA_signal 7139..7363
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
primer_bind complement(7378..7394)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(7379..7395)
/label=pBluescriptKS
/note="For pBluescript vector"
primer_bind complement(7419..7438)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(7420..7438)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(7448..7465)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(7448..7464)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(7457..7479)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 7606..8061
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(7693..7712)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 7903..7924
/label=F1ori-F
/note="F1 origin, forward primer"
promoter 8087..8191
/gene="bla"
/label=AmpR promoter
CDS complement(8680..9339)
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/label=CmR
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPELRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
primer_bind 9254..9273
/label=CAT-R
/note="Chloramphenicol resistance gene, reverse primer"
promoter complement(9340..9442)
/label=cat promoter
/note="promoter of the E. coli cat gene encoding
chloramphenicol acetyltransferase"
rep_origin 9662..579
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
61 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
121 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
181 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
241 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
301 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
361 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
421 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
481 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
541 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
601 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
661 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
721 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
781 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
841 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
901 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
961 cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg
1021 gaacaaaagc tggagctcca ccgcggtggc ggccgctcta gaatatagaa gccaaggatt
1081 tcaagggttt ccttttctct cttcttcttt ttttttcttt ttctttttcc tgagatggag
1141 tttccttttg tagccctgac tgtcctggaa ttcactctat agaccaggct agcctcacac
1201 ttagtgatct gcctgcctct gcctcttggg tgctcaggat tcaaggcatg aaccaccact
1261 acccgaccag ggatttctta cacacttctg actggactaa ccaggaaagc agagagggag
1321 acaggaagaa aatgctcaga aggaaggagt aggattggag gtgagctggg ggaacccaga
1381 ctgagccgtg cagaagacaa ggaagaagaa agccacccac acacctagga tccacccaca
1441 gattttgctc tgggtacccc tgtctggaga ctgtagggct ttgtgatgga gggtggggta
1501 tgcttcatgc cccgtgccct ttactccaga cctaaatgcc cacccccaca tacagctgct
1561 cgctctctct ctcccctgcc cttctcccaa gagaccagtt ctccatccct ggtctgcagc
1621 caaggctggg ggcagaagaa ctttctggag gatttgagtg agaaaagcaa gagagcctca
1681 agtagggact ggaacctctg ggaagggagt gcagaggaga cccgggtatg tgccctacct
1741 ggtacattta tacctgggca gcctctgctc ctgttccaga cttcagagcc cagacgggtc
1801 ctctccctcc ctcatgaggg gaaacatttg gggaaatttg gagagagaca gaactcagag
1861 ctcagcactt tcctctttct gtttttcttc ttgaggaatt ttttccccca actgctgatg
1921 actttaccat tcttgggggt gggggggtgg agattctggc ttttgctccc cctacactcc
1981 aagtgccgga caaagcccta cattccacaa gaagccaggg cttcagagtt tcctaaagat
2041 gaggtggcgt ggcgagtctc ctccctctcc cagctccaac tccccctccc ccagtctcca
2101 gccctcagcc tggccaggga ggccccgcca ggctgggagg agaccccaag cacattcttc
2161 ctctcgctgt catgctgcag aaattaaaga cacatctctg agctgggtac ccgccaatcg
2221 tttcaagttg agaagtggca gaggaggtcc cgagcttcag ctccatgcca cgtgtaaagg
2281 aagcttggaa cccactgccc acaactcctg gggcaaaaac ctggagtcag acatggggtg
2341 aaggctgtca cacggcacag acacgtcaag cacccccccc aattctagta gtctcctagc
2401 ctccaccaga accccagacc cttgatgtgg cagtcaccag tccacacctg ttaggctctt
2461 gtctcttctt ccagatgagc ctggggggcg ggggggtgct agatcaggag cagggaaaag
2521 tagctttgga taagtgcttt tcccaataca aaacccaaca aagagtgggc agatcacact
2581 gtgtagtgct tcgtggaacc ctaccctaga caactgcctt gaacacctat tccctctgat
2641 gtacaccatc cccgtccact gttagggagt gggcatcctt tggaactgac cactgtggaa
2701 ggcaggactt tactgagttc cggaactacc atctcagctt ctcagcccca gccttaccct
2761 acaggcactg gcataggcgg gggcagatcc tgggccacaa gtcactgcca catggttggg
2821 ataattgatg aagtcctgtc cttccattgc tgtctccagt tctgcttctc tggaaactct
2881 atatttttcc ctttaattat agcctctgca gtctccctct gccaccccac ccgcaccgct
2941 tagcctaact gcccacggcc agcgacgtgg ctccctcccc ttctgctccc ttggtctttt
3001 ttattttttt ttctttgcct tcgttgcaca aaactagctc agggagggcg tgaagggggg
3061 gggagcaatg gaatcttgga tggtttggag gaggcgggac tccttgcttc cacgtttaca
3121 gctctgaaga cggctgtggg ggaagtgata caggacgtct atgggccctg agaggagacc
3181 cctatgcttc cctgccaccc acacagttta acaaaatgaa gttcctaagt agagtggggg
3241 tcaggcagag cacctttgca gggttgatgg gagcccaggg aaagaaagga cactgtcttt
3301 tagggacaca tttaaatata agccactttt cttgggggac gacaaatgac cctttcctga
3361 ttgcagaggt ggggaacaat ggctgagatt ttcagcaaag aagcgaggac atgaggagta
3421 gccttcaaat aaagtcactc agctaccaaa aacaagtttc tgccacacac cgagttcacc
3481 taggtgtccc cagaccagat ccaagtacag taaggaaagc aggttctcta cagagagaac
3541 acggctctat ggccaatgcc ttctacctgc tctttctgga ttgatactgc tacctaagag
3601 ggcctctaac caattcctgg ctgtagccac agctgacaca agaccttttt ctaagacatc
3661 cctggtcaca ggcctcctgt agcaaattcc agccctggga tggaggtggt caggaaagag
3721 tttatacaag aagacccagg ccacagcttt aaggactcag aaacccccct gcccacacgg
3781 ctgcccatca taacgcagaa ggtttcttct ggaaggacaa ggatgtcaaa cttctcccca
3841 agcctaatcc tcagagatgt ctccctctgt tacacctggg gctggagaaa ggtgggtctt
3901 tcatggagcc acattcatgg cagaacagat agccacccca ctcctttcaa acaaccacat
3961 atctgactct tagtatctgt gaagagatgt ctaatttgtt cccaaatatt cctaccctgc
4021 atacctgggc ccacaccatg aggtattctc ctccctctaa cagtcacatc tgcttagctg
4081 cctggttctt cggatttgga gagatgcttg cctaacttat tcttccttag gtcttcccaa
4141 ggatgccaga aagactatga gacatggcca agaggacctt ttcccaattg tgcctgacac
4201 tgaacccttt gtaatgttcc ccaactcaga ttcccaattc tacatccttc tgatttgagg
4261 tcccagaagg aaagtgcaag gggcatcccc tacccacaat cagtatatcg agggcccagc
4321 cacactcagt gatagcacct ctggcccatg tagatctggg ggacaagggt ggcagaattg
4381 caaagggggg agggggctgg gtggactcct ttcccttcct ttccctcctc ccccctcttc
4441 gttccaaatt gggggccggg ccaggcagtt ctgattggct gggggccggg ctgctggctc
4501 cccctctcca agaggcaggg ttcctcccag ccctcctcca tcaggatggt ataaaagggg
4561 cccaggccag tcgtcggagc agacgggagt ttcacctccg gacggagcag gaggcacacg
4621 gagtgaggcc acgcatgagc cgaagctaac cccccacccc agccgcaaag agtctacatg
4681 tctagggtct agaggcggcc gccttcagct ttgtggacct ccggctcctg ctcctcttag
4741 gggccactgc cctcctgacg catggccaag aagacagtaa gttccaaact ctggggaggt
4801 tatggtttct tgctatcgcg ttctgcatag gggtcctagc aataaagact taaacggaag
4861 cgcggggtgc tccagagtta ggagggatga gaaggtgatg gagaggaact gggaagagag
4921 aggtcaccct cgcgagcaga ggagagtgag cggatctcag aagtggagcc ctggttgaga
4981 tggcacggca ggaaatttca agcatctatc tgtagcgcga cgggaaaagt gggaatctgg
5041 gcaggggttg ggtcagagta cgtcgaagat caaataagac ctctggaata gagattcgaa
5101 gctcaagatg taaaagcaat caaaaaggct gcgggatgat tcataaggaa agattgtttg
5161 ccctctctgc aggctagaca gtgttgctgg ggcctccggg gtgctgggct ctgggagtgg
5221 ggtgggggga ggaagggacg cacggagagg gcgggttgct ggatgagaaa gtttggctaa
5281 gacttgactg ggcgcggtcc ctgtgcctcc tgctggtcaa ccttgagcac tacgagtcct
5341 ggtacagtac tttacagcgc gcgggcttaa gagggaaaac ctgggctatt gccgggcctg
5401 gaccctcatc tccagactag gaaaataaaa gcacagatgg atgtgatagc tttggtcttt
5461 gaaagtgacc caggccagta aaattgggtc cgccccgtgc attgcttagc gttgcccgcc
5521 acctagtggt tgctttgatc cggtgctaga gaggtgggcg tggttaacta atcattgcta
5581 tttgtagact tgagttcttt ctttggctaa ggagtgaccc caaagctcta ctggctttga
5641 aggaaggacc gggcaagctt tgattcctgg gagcggagag ttagggtgta tctaacttta
5701 gggatatatt ttactactct ggacaaggct ccacggaagt caccctcctc cattttagcc
5761 ccgcccattc cgtcccacct gcccaaggaa aggaggtggg accagggctg gaatttccct
5821 tctctcctcc cacccccgcc tccggtctcc ccgcaaacca cgtcccacaa acttccacct
5881 actttgtagg agccctccta ctctccgtca ggaggacttc tatttcattt ggttcccgcg
5941 cgcgtgggct gaagttgagt ttggaagcaa ccccagtcta aagctttgtt taaattcctg
6001 ggaacttgga agtaacagcc ctggccaggc ttcttggtgc tgtcactgaa cgccgttaca
6061 gagcagacct gcatttaagg atttaaggga agaaaatagg ggttggggga agatgtggga
6121 agtggaaaat ctacgtgcac ctcttagaca ggggaaaggg tgagtaaggc tgggatccgg
6181 tggtggtgca aatcaaagaa ctgctcctca gtggatgttg cctttacttc taggcctgta
6241 cggaagtgtt acttctgctc taaaagctgc tgcaggtctt agttaactaa gactctagaa
6301 ctagtggatc ccccgggctg caggaattcg atatcaagct tatcgataag cttggcattc
6361 cggtactgtt ggtaaagcca ccatggtgag caagggcgag gagctgttca ccggggtggt
6421 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
6481 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
6541 gctgcccgtg ccctggccca ccctcgtgac caccttcggc tacggcgtgc agtgcttcgc
6601 ccgctacccc gaccacatgc gccagcacga cttcttcaag tccgccatgc ccgaaggcta
6661 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
6721 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
6781 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
6841 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
6901 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
6961 cgtgctgctg cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa
7021 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
7081 catggacgag ctgtacaagt aactcgctag agtcggggcg gccgggcgag ctcgaattct
7141 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
7201 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
7261 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
7321 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggaattccg agcttatcga
7381 taccgtcgac ctcgaggggg ggcccggtac ccaattcgcc ctatagtgag tcgtattacg
7441 cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
7501 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
7561 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg
7621 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
7681 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
7741 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
7801 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
7861 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
7921 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
7981 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
8041 aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat
8101 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
8161 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
8221 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
8281 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
8341 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
8401 ttgatcagaa aaaaaggatc atatcgtcaa ttattacctc cacggggaga gcctgagcaa
8461 actggcctca ggcatttgag aagcacacgg tcacactgct tccggtagtc aataaaccgg
8521 taaaccagca atagacataa gcggctattt aacgaccctg ccctgaaccg acgaccgggt
8581 cgaatttgct ttcgaatttc tgccattcat ccgcttatta tcacttattc aggcgtagca
8641 accaggcgtt taagggcacc aataactgcc ttaaaaaaat tacgccccgc cctgccactc
8701 atcgcagtac tgttgtaatt cattaagcat tctgccgaca tggaagccat cacaaacggc
8761 atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc
8821 catggtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat caaaactggt
8881 gaaactcacc cagggattgg ctgagacgaa aaacatattc tcaataaacc ctttagggaa
8941 ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta gaaactgccg
9001 gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct catggaaaac
9061 ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca ttgccatacg
9121 taattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt
9181 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt
9241 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga
9301 tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga
9361 aaatctcgac aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt
9421 ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc
9481 ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat
9541 ttattcggtc gaaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
9601 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
9661 cttgagatcc t
//
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