| 质粒名称: | pSR47 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 69258 |
| 存储实验室: | Sander van den Heuvel |
|
载体骨架基本信息 |
|
| 载体名称: | pCFJ355 |
| 载体出品公司: | Erik Jorgensen |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 8941 bp |
| 完整质粒大小: | 12454 bp |
| 载体修饰: | -- |
| 载体类型: | 蠕虫表达，Cre/Lox；莫西 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Cre recombinase,Cre recombinase |
| 物种来源: | C. elegans (nematode) |
| 插入大小: | 1134 bp |
| 基因突变: | codon-optimzed index 1.0 |
| GenBank编号 | -- |
| 启动子 | myo-3 |
| 标签/融合蛋白: | 人细胞色素c氧化酶亚基VIII（N端） |
| 克隆方法: | Gibson克隆 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | M13 forward |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pSR47 was a gift from Sander van den Heuvel (Addgene plasmid # 69258 ; http://n2t.net/addgene:69258 ; RRID:Addgene_69258) |
| 参考文献部分: | G1/S Inhibitors and the SWI/SNF Complex Control Cell-Cycle Exit during Muscle Differentiation. Ruijtenberg S, van den Heuvel S. Cell. 2015 Jul 16;162(2):300-13. doi: 10.1016/j.cell.2015.06.013. Epub 2015 Jul 2. 10.1016/j.cell.2015.06.013 PubMed 26144318 |
买家导航
pSR47载体质粒基本信息
pSR47质粒图谱载体图谱和pSR47载体序列质粒序列多克隆位点信息
pSR47质粒载体简介
pSR47质粒序列载体序列
LOCUS pSR47 12454 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION pmyo-3::CRE for integration on ttTi14024, Chr X.
ACCESSION .
VERSION .
KEYWORDS pSR47
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 12454)
AUTHORS Ruijtenberg S, van den Heuvel S
TITLE G1/S Inhibitors and the SWI/SNF Complex Control Cell-Cycle Exit
during Muscle Differentiation.
JOURNAL Cell. 2015 Jul 16;162(2):300-13. doi: 10.1016/j.cell.2015.06.013.
Epub 2015 Jul 2.
PUBMED 26144318
REFERENCE 2 (bases 1 to 12454)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..12454
/organism="synthetic DNA construct"
/mol_type="other DNA"
protein_bind 15..35
/gene="mutant version of attB"
/label=attB4
/bound_moiety="BP Clonase(TM)"
/note="core recombination site for the Gateway(R) BP
reaction"
protein_bind 1795..1819
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
CDS complement(1965..1976)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind 3916..3933
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 3917..3933
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
CDS 6050..6124
/codon_start=1
/product="nuclear localization signal from the C. elegans
EGL-13 transcription factor (Lyssenko et al., 2007)"
/label=EGL-13 NLS
/translation="MSRRRKANPTKLSENAKKLAKEVEN"
terminator 7278..7601
/gene="tbb-2"
/label=tbb-2 terminator
/note="transcription terminator for the C. elegans tubulin
beta-2 gene"
primer_bind complement(7642..7658)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(7642..7658)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
protein_bind complement(7665..7689)
/gene="mutant version of attB"
/label=attB2
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
protein_bind complement(10119..10139)
/gene="mutant version of attB"
/label=attB3
/bound_moiety="BP Clonase(TM)"
/note="core recombination site for the Gateway(R) BP
reaction"
rep_origin complement(10276..10864)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(10356..10375)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(11035..11895)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 11658..11677
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(11896..12000)
/gene="bla"
/label=AmpR promoter
primer_bind 12068..12086
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(12124..12146)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 12246..12265
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
ORIGIN
1 atccgccaag ctatcaactt tgtatagaaa agttgatgct tttacatctc catcctagct
61 cacttgtcat actattaccg ttcccatagt aattgtacaa tgcccccgtt ttatcaagca
121 tttctttttt ttttttgaga atttttaaat taatcaatat cccgagttct tccacctttt
181 gagcaagtct cgttgaggat tgcattggtc ccagcgctaa ctaggcgtca attcatccac
241 ctctccattc tctctcccgt tccaactgtg tttttctttg tcagcggcga attttccaca
301 accaatcgag tcctatttct ctttttttcc cccacctccc aataatttta tttgcatgtt
361 ttataaactc actttgttca ataatttatt gattttattt tttctatgat ttgaaacttt
421 acatttttcc tactcttcta tagcaatact ttgtcactta caataacccc ccccccccag
481 tctgcctata tttatttaga tgttctttat ggccacccaa tgtataaact cttttagtgc
541 atttgtaggt tctttcttca tcccacccaa ttccccctcc accacaaatc ccagttttga
601 ttattttaag cccatattta taagaagtaa atatatttat agatttttct tagattataa
661 gctcttctag gttttccctc gttgagaaga atgatctgaa aaagcctaaa ttatagattt
721 ttgaaacgta ttgtgtagaa tttgtattta ttcgtgaaag tggaataaag ttaaaacgga
781 aaaatgtagt attagttttg tgcaaaacat tggccctata aatgtagatg taggaaagtc
841 tgtttattct tggaaaaatc cctaattttt gtgaaattgt ttggtttgca gcggtcggaa
901 gcaatcttga aaataattat catttgtaat cacattttgg taatttatta cgtttatttt
961 cttcattgat tggtaggtat atgttttctt tcgaaagatg aacaaatgat agtgaattgg
1021 tgcacctata aacggaaaat ataccttaaa caattaatgg ttaaaaaatc cacaatgaag
1081 aaaagatatt agaaaattcc ggttgctaag acattgaaaa aaacttgaaa aaagactttg
1141 aaaacttgac aaaatttggc acttttgaaa agaacccatt taaaacttaa agcatgtact
1201 aaaacgagaa ctttatttga aaattattat atgtgtattg ctgcgcttgt acatttaaaa
1261 aaaggaacct tttcaattga tacttaaaac aaatagaagt tttttaatcc atatcctcaa
1321 gcttcgcaga taaaccctca atatgccaaa ctctaataag tagcaatgta acatgttttt
1381 ggcgctggtg agtaggtact aaaaatttaa taaatgtttc cgatttaaaa ttttagatct
1441 cgtcagaatc gttgtcaacg cagaactaca attctggcat acttttgtta taacgatatc
1501 aaataccaaa gaactcgtcg aaattgcata cttgttgatg gaaccatccg ttcaactgaa
1561 cgccagccag tttattactt aaatgaaaac taatttttca attgatgacg gttagtgact
1621 ttaggtttca tttgatcagc gtagttgctg acacttaatg gttcgggaac ataaaagaac
1681 atgtcgaaca cgtacatgac gactgcttaa aagttgaaaa tttcccatat ccgtttctca
1741 ttttatgtag tctctttcag tgtggctact aacgattttg caattttaca ttatacaagt
1801 ttgtacaaaa aagcaggctt gagccaattt atccaagtcc ttgtaaaaag tattcgaaaa
1861 ttgttaacgg ataaaatgtt tattataata tcaaaaacaa tttgtcagtt gaccactttt
1921 tgatatattt gacagaaacc gggatgaatt ggctcaaagt tagggcgccc ttctattaca
1981 gggtttctga taacaaaccg gttattaact cccaacaagg gatgatttca attcatcatg
2041 ctcaaattga cccaaattaa gttacatgac aaattcatcg cccttttcaa ctcttcttgg
2101 ctcatcatct gttattctgt tcctattctg tctgcaccct ataccccttt gcatactctc
2161 tcgtcattcc tttcttggat agtgtcttct tctccccagc tctgctactt ctatgacttg
2221 cgcccgctgc ttttccgctc gttcctctcc tctctgacgt cttcttctgc ttcttttctc
2281 cttccttttt cccttctcaa tcttcctctt tttcccattt ctgtcaatca ttcgaagaag
2341 aagaagaaga agaccctcat tcatttcatt tttttttctg tcggtgtgtg ctgctcggtt
2401 aagagtgagc tctctattcc acgtcttctt ctttttcttc ttcgattcga atcaatcact
2461 ccaccaaaac gcattcgttt ttgggattca ccccgcggtt cgcaataggt ttctttttca
2521 aattattagc gttataaata gaaaaatggt ggagtttcaa taaaaatgat aatttacaaa
2581 gtgtattttg attacatgta ctcaaaaagg ttgaaatttt tcataccagt tttccggaaa
2641 tccatctgat atcattatcg tatttttctt tttaaaaatg ttttcaaaaa aaaacaaaat
2701 atagctggtt atttggcacc ctctaattac cattttcctg tcacaccaca ctcttttcct
2761 tcttccactt ctttccgttt tcagccgctt ccaaccaaac cgatatgaaa gccgagcaac
2821 aacgatcgat tccacccggc tcggcgacct tcccgtcgca ggtgagactc agaaaactag
2881 agaaaccgct caactaactc ttgatatccg atttcattct tttctctttt ctttttgttg
2941 aacttttcca tatttccaga tgccacggcc accaccaagc accgaacaag gaatcacaac
3001 ggaatcggag cttgcgaaga aagctcaaat cactccgaac gacgttttag cacttccggg
3061 aatcactcaa ggtatcttgc tccctttgga tttcagttta aaacataaat ttaggattct
3121 tatgctcccc atctgcgaac atctataaca tcgagttcac caagttccaa atccgtgatc
3181 tggacactga gcaagtgctt ttcgagatcg ccaaaccgga gaacgatcag gagaatgatg
3241 agtcgccaca ggagtcggca agatacgtgc gttatagatt tgctccaaac tttttgaaac
3301 tcaaaacggt cggagcaact gtggaattca aagtaggaga catcccaatc catcatttcc
3361 gaatgatcga acgtcacttc ttcaaagatc gccttctgaa gtgttttgac tttgaattcg
3421 gattctgtat tccgaattca cgaaacaact gtgaacatat ctatgagttc cctcaactct
3481 ctcaacaact cagtgagtca ttattctaaa aagtacaatt caaaagacta atctcttttt
3541 cagtggacga catgatcaac aatccaaacg agactcgttc tgacagcttc tatttcgtcg
3601 ataacaaact cgtcatgcac aacaaagccg actactcata tgatgcataa atatttaata
3661 caaaaatgtt ctggataatt attctgtcga atagaaaaaa aactccaaat gtgattaaat
3721 tccaataatt cctgtctagt ttgttccttc cccttcccct tctcatgttc aatgcattcc
3781 taagcttttc agttcccccc ttgtttctat attttttcgc tgtcctgtca cactcgctaa
3841 aaacactaat cacacggaaa tctgttttca ataaaaactc caactttaac tcattttcaa
3901 tttcaactgc acgggtgtaa aacgacggcc agtccgtaat acgactcact taaggccttg
3961 actagagggt accagagctc acctaggtat ctagatgtgt gtgattgctt tttcacaatc
4021 agtgttttca ggattatgtg atgaactaga tcttcaagtt tcgttacatt tcatatgttt
4081 tcggaactca cgaagtacat attgggtatt gtgctcaaaa aattcagcaa tcagcttcgc
4141 tccgctgact ttagaaccca aaaaaatagt atggccaaac tgactgtgtt acgatcattt
4201 caatttttca atacatattt aagatttcta agagtaagaa ggtcaaaaac tgttctggaa
4261 tacatatata tttttcaggt tacaattagt caaaaagtgc actgaaatat acgttttaat
4321 ttcacgaata acccaattag ttcaatgtat ttttggtcaa ccaacgttaa agtttggctt
4381 ccaaccaatt atcatttctg atcaaccaca atgttttttc tttatctgca agttaatttt
4441 ttatttttat ccagatgttt ggcatatttt tcaattcttc actagcgccc acttcttgca
4501 cttccggcgc cctgaatcta atgcatctgt tgcaagaatt gaaagaccaa tcaacacatt
4561 gttttcttca cgagatactg aagaaaatga ataaaaacag agaaaaagag ccatgtgatt
4621 agtgacaact gttgctaaca gataccatag cttggacttg gtacgtgatg gcaacgtatg
4681 ggtcaacaaa aatgattgca gagggggtgc aaaacagtca agtcgagaaa atatgaaaaa
4741 cagaaaacaa agaacagaaa aatgggtttg agagtcagta taatttataa aagaaaaatt
4801 gtacatagaa attaaccatt tttgtagaag aagttatttt tcaagcatcg ttaaaaatta
4861 ttcaaagcac cttatttcat atttaatttt aaacatggtt aaatgaacaa cacggtgcgc
4921 aatcaggaaa acttgaaatc tgaaactgtt gttgtgatct tcttcgcaac tgttcagata
4981 gcactagtgt aatgttaaga gtgcgcgaat ataatggaat ataatggatc acacctcctg
5041 ccatcaggta aacgtctctg ttatcacata tttccaacta ttaaattttt accttttaca
5101 gttttacatt tttttgaaaa aagtaacttt ttgtcttcaa aatccctgac gaaaatatca
5161 aatattttaa tcgagactgc agaggaaccg attgatgatt tggaaaatcc agctttacct
5221 gtgtaagaac tgaaaagttt cataacccta gggtattccc agttacattc cccactggct
5281 aacaatagca cccagttttt catcaccttt cttcaaattt ctcggcgatt tgttaaaaac
5341 aaaatttgtg tcccttctct gatatctcta tgtctctaaa cacaagttca tcggaaaacg
5401 aaggagggta ggtgttggtt gggctcccga agtgaaaata gaagagcaag aatagaatat
5461 tagagagaga gtgcagagag ggcgggatag ctcccgggat tccgttttct tcttctttat
5521 cttcaacgat gatgtgtgtg cgtgttgtat agattctgtt gctcccccac aactcgctcc
5581 gaaggctcaa tacaattcaa ttgatattgg aggagagcct accggagtgg gaggataaga
5641 agaaacataa gaagaagaag aagaagaagc atgcttctgg tttttgatgc tatgaaaacg
5701 gcacaaaaag atgattgagg tcccttttca ataccttctc tcatctttca aatcccattg
5761 aaacctaaaa cttctcacca cgctttacca ttgttctcca aaaacttata gcaatgtcta
5821 taactttttt atctctgaaa agcagtgttc catttttctt tttcctattt tatttcaatt
5881 gtttctcaca tttcgtttgg attctttgct tgtcaaccag cttcttcttc cacttttacc
5941 gtctaatttt cagggcaggg agccatcaaa cccacgacca ctagatccat ctagattaat
6001 taaaatggat tacaaggatc acgatggaga ctacaaggac cacgatggaa tgtcccgtcg
6061 tcgtaaggcc aacccaacca agctctccga gaacgccaag aagctcgcca aggaggtcga
6121 gaacggagga atgtccaacc tcctcaccgt ccaccaaaac ctcccagccc tcccagtcga
6181 cgccacctcc gacgaggtcc gtaagaacct catggacatg ttccgtgacc gtcaagcctt
6241 ctccgagcac acctggaaga tgctcctctc cgtctgccgt tcctgggccg cctggtgcaa
6301 gctcaacaac cgtaagtggt tcccagccga gccagaggac gtccgtgact acctcctcta
6361 cctccaagcc cgtggactcg ccgtcaagac catccaacaa cacctcggac aactcaacat
6421 gctccaccgt cgttccggac tcccacgtcc atccgactcc aacgccgtct ccctcgtcat
6481 gcgtcgtatc cgtaaggtaa gtttaaacat atatatacta actaaccctg attatttaaa
6541 ttttcaggag aacgtcgacg ccggagagcg tgccaagcaa gccctcgcct tcgagcgtac
6601 cgacttcgac caagtccgtt ccctcatgga gaactccgac cgttgccaag acatccgtaa
6661 cctcgccttc ctcggaatcg cctacaacac cctcctccgt atcgccgaga tcgcccgtat
6721 ccgtgtcaag gacatctccc gtaccgacgg aggacgtatg ctcatccaca tcggacgtac
6781 caagaccctc gtctccaccg ccggagtcga gaaggccctc tccctcggag tcaccaagct
6841 cgtcgagcgt tggatctccg tctccggagt cgccgacgac ccaaacaact acctcttctg
6901 ccgtgtccgt aaggtaagtt taaacagttc ggtactaact aaccatacat atttaaattt
6961 tcagaacgga gtcgccgccc catccgccac ctcccaactc tccacccgtg cccttgaggg
7021 aatcttcgag gccacccacc gtctcatcta cggagccaag gacgactccg gacaacgtta
7081 cctcgcctgg tccggacact ccgcccgtgt cggagccgcc cgtgacatgg cccgtgccgg
7141 agtctccatc ccagagatca tgcaagccgg aggatggacc aacgtcaaca tcgttatgaa
7201 ctacatccgt aacctcgact ccgagaccgg agctatggtc cgtctccttg aggacggaga
7261 ctaagcggcc gcgataaatg caaaatcctt tcaagcattc ccttcttctc tatcactctt
7321 ctttcttttt gtcaaaaaat tctctcgcta atttatttgc ttttttaatg ttattatttt
7381 atgacttttt atagtcactg aaaagtttgc atctgagtga agtgaatgct atcaaaatgt
7441 gattctgtct gatgtacttt cacaatctct cttcaattcc attttgaagt gctttaaacc
7501 cgaaaggttg agaaaaatgc gagcgctcaa atatttgtat tgtgttcgtt gagtgaccca
7561 acaaaaagag gaaactttat tgtgccgcca agaaaaaagt ctcaactagt gagtcgtatt
7621 acgtagcttg gcgtaatcat ggtcatagct gtttcctgcc cgtgacccag ctttcttgta
7681 caaagtggtt tgtttgtgag tttcaggaaa cttgtgaatt cgttcaaacc cttcagaatc
7741 agaataaaaa atggtgcttt gtatttctta aattttaata actttttcac gagcactaca
7801 ttttaaaatt ttgtagaagt tgtctgtttg cacccattta atttcaaatg tgtcataagt
7861 caacaactgg cacgttgagt atacatagaa aagaatgatt ttacctttta atattttttt
7921 tgttataaaa gcttgcaaca tttttattac ctctatggat aaaaaacgaa ggctaggaga
7981 aagaatctaa aaactcggat tagtttttgg atacaaaaca gaagattaga atatttccct
8041 tcacgaattt tgagggtaaa acaattttac cgaagttttc aaaggttttt gttcaagatt
8101 ccattcattt tttttgaaat acttcataca cgtggtgtca ggttgttacg gtttgactta
8161 caaattttgc ccaaaaaatg agacgtcagc acgtttttag ccatttaaat cagttaagaa
8221 ctctgtgtct caactcccgc attttttgta gatatttgta gatcaaaccg aaatgagaca
8281 ctctgacacc acgagttcat attgtgaaac tctcttgaat acttatcact gtgccaaccc
8341 atgtcacact tttaaataca ttgaacagta taaaaaactc aggtcaaaag taacatttga
8401 ttgatccccc ccatttcctt ctgaactttg cttctcctgt ttttgcgtcg tttcgccatc
8461 atcattaaag tgactcttgg acttgagact ctagttgctt atcgattggt gattgaaaaa
8521 gtaggagaaa cgaatctttt catctactcc tcttctgcac ccgccttctc ttcctcttct
8581 tcatccagca gtgccatttc tcttcggcag caagaatggt gtgtaaagtg atgacggcgc
8641 agtttcttct ggcacaggag tagcacagca caacctcagc agtagttgga gcaacccagc
8701 agcagcatat tttcgatact catcgagcac ttgccgttgt gttgtgtcat ccgccaacaa
8761 atcagcaaac aacgtttttt atcccgatca ttattttttt tcctcgctct gggttggact
8821 tgtctatgtt tcaattttta ttacaaaaat aatggaagaa aaacatttgt ttgaaaattt
8881 taatccctac tttaaagtta tattatatat agaaaataag tctaaaaaat gttttattat
8941 ttttttaaat tttatttaaa attttgaaaa aaatttcgta tggctgatgt cccagaccca
9001 tcattttttg tagttttctg tttttctgaa aatttaaatg accttctccg gtttttctaa
9061 gatgacgtca catttcaaaa acccattgaa actaattttc agaaaacgga aaactcaata
9121 aaactaagcg tgaaatggaa aaaaaatcat ttaaaaagtt agaaaaattt aaaaaaaaga
9181 tttcaattct actcggccat tgcgatgaat tttttatcaa ttaacgctaa atctgtaaaa
9241 attagattaa aaaccaaata ataaagaaac atacaaggaa tttcaatttt aaaaattgtg
9301 tcatcaaact ttttgagaaa cttcagtatt aatttaattt catggaatgc ggttttttct
9361 ctaacttttt aacaaaacta ggtagattaa aaaatttaaa caagaccata tataagctac
9421 tgtttcaaat ctacctctgt atgtatgtac aacaactcct tcaaagtgaa cgtatttgcg
9481 aaaaatgggc tcatcacaga agctcatcat tttttacaac attgtattat aaacttgcca
9541 gtgcgtagcc agcattttat ttaactttct atgaattttt gatatttttt gaaaaagatt
9601 ttctcacatg gttctcaatt gaacactaaa acataatcta aaaactacga attttgagtt
9661 taaagttttt cagttttaaa ggtggagtag cgccagtagg gatttagtct aagtacaatt
9721 ataatgacct aaaacaacca aatatcataa caaaacactc cgaaaatttt cagatttttt
9781 cataatttcg gagctttggc aaattgccaa attttgaaaa atatgagctt ttgaggaaat
9841 ccaaagcaat gtcgcatgtt ccgaccctta caatgtttca atacaagtaa tcaaaacaaa
9901 attaaaatat aaaaaatgta gaaaaaaatg ttttttttgg tcgtcttcca aaattatgag
9961 tggcaaaaac tgagtaattg tcattttttg acaggaaata aaaaaaattc aaaaattttt
10021 tgaaaatttt tattatgata tttgaatatt ttaggaccaa aggagtggtt tttaacagtt
10081 tccccacatg cgctactcaa cctttaaact tcatccccca actttattat acatagttga
10141 taattcgatg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
10201 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag accaggaacc gtaaaaaggc
10261 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
10321 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
10381 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
10441 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
10501 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
10561 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
10621 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
10681 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
10741 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
10801 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
10861 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
10921 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
10981 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
11041 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
11101 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
11161 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
11221 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
11281 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
11341 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
11401 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
11461 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
11521 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
11581 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
11641 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
11701 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
11761 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
11821 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
11881 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
11941 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
12001 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
12061 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga
12121 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
12181 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
12241 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
12301 cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg
12361 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
12421 tgctgcaagg cgattaagtt gggtaacgcc aggg
//
