| 质粒名称: | pBAD-HisD-Thyone |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000363 |
| 产品用途: | Express green fluorescent sensor Thyone in E.coli |
| 存储实验室: | KIRYL PIATKEVICH Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pBAD |
| 表达系统: | 细菌表达 |
| 启动子: | araBAD |
| 拷贝数: | 低拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a，TOP10 |
|
插入基因信息 |
|
| 插入基因名称: |
Thyone
|
| 物种来源: | Branchiostoma lanceolatum |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Genetically encoded green fluorescent sensor for probing sulfate transport activity of solute carrier family 26 member a2 (Slc26a2) protein
Lai C., Yang L., Pathiranage V., Wang R., Subach F.V., Walker A.R., Piatkevich K.D.
Commun. Biol. 2024 71 7, 1–13 (2024)
|
买家导航
pBAD-HisD-Thyone载体质粒基本信息
pBAD-HisD-Thyone质粒图谱载体图谱和pBAD-HisD-Thyone载体序列质粒序列多克隆位点信息
pBAD-HisD-Thyone质粒载体简介
pBAD-HisD-Thyone质粒序列载体序列
LOCUS pBAD-HisD-Thyone 4710 bp DNA circular SYN 18-MAR-2024
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4710)
AUTHORS Kiryl Piatkevich
TITLE Direct Submission
JOURNAL Exported Apr 15, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..4710
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 120..285
/gene="araBAD"
/label=araBAD promoter
/note="promoter of the L-arabinose operon of E. coli; the
araC regulatory gene is transcribed in the opposite
direction"
CDS 319..327
/codon_start=1
/label=Start
/translation="MGS"
CDS 328..345
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
primer_bind complement(336..359)
/label=VECTOR-R
primer_bind 344..363
/label=RL26-F
CDS 355..1062
/codon_start=1
/product="bright monomeric yellow-green fluorescent protein
derived from LanYFP (Shaner et al., 2013)"
/label=PB26 mNeonGreen-4
/translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYE
ELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFED
GASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWIRAKKTYPNDKTIIS
TFKISYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQ
KAFTDVMGMDELYK"
regulatory 355..358
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
regulatory 610..619
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
primer_bind complement(1049..1076)
/label=RL26-R
primer_bind 1061..1090
/label=VECTOR-F
terminator 1281..1367
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 1459..1486
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
promoter 1505..1596
/gene="bla"
/label=AmpR promoter
CDS 1597..2457
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 2628..3216
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature 3402..3542
/label=bom
/note="basis of mobility region from pBR322"
CDS complement(3806..4684)
/codon_start=1
/gene="araC"
/product="L-arabinose regulatory protein"
/label=araC
/translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
EKVNDVAVKLS"
ORIGIN
1 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct
61 tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca
121 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg
181 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg
241 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg ttttttgggc
301 taacaggagg aattaaccat ggggagccac catcaccatc accatggcct cgagatggtg
361 agcaagggcg aggaggataa catggcctct ctcccagcga cacatgagtt acacatcttt
421 ggctccatca acggtgtgga ctttgacatg gtgggtcagg gcaccggcaa tccaaatgat
481 ggttatgagg agttaaacct gaagtccacc aagggtgacc tccagttctc cccctggatt
541 ctggtccctc atatcgggta tggcttccat cagtacctgc cctaccctga cgggatgtcg
601 cctttccagg ccgccatggt agatggctcc ggataccaag tccatcgcac aatgcagttt
661 gaagatggtg cctcccttac tgttaactac cgctacacct acgagggaag ccacatcaaa
721 ggagaggccc aggtgaaggg gactggtttc cctgctgacg gtcctgtgat gaccaactcg
781 ctgaccgctg cggactggat cagggcgaag aagacttacc ccaacgacaa aaccatcatc
841 agtaccttta agatcagtta caccactgga aatggcaagc gctaccggag cactgcgcgg
901 accacctaca cctttgccaa gccaatggcg gctaactatc tgaagaacca gccgatgtac
961 gtgttccgta agacggagct caagcactcc aagaccgagc tcaacttcaa ggagtggcaa
1021 aaggccttta ccgatgtgat gggcatggac gagctgtaca agtaagaatt cgaagcttgg
1081 ctgttttggc ggatgagaga agattttcag cctgatacag attaaatcag aacgcagaag
1141 cggtctgata aaacagaatt tgcctggcgg cagtagcgcg gtggtcccac ctgaccccat
1201 gccgaactca gaagtgaaac gccgtagcgc cgatggtagt gtggggtctc cccatgcgag
1261 agtagggaac tgccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
1321 gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg ccgggagcgg
1381 atttgaacgt tgcgaagcaa cggcccggag ggtggcgggc aggacgcccg ccataaactg
1441 ccaggcatca aattaagcag aaggccatcc tgacggatgg cctttttgcg tttctacaaa
1501 ctcttttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
1561 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
1621 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
1681 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
1741 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
1801 acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa
1861 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
1921 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
1981 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
2041 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
2101 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
2161 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
2221 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
2281 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
2341 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
2401 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
2461 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
2521 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
2581 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
2641 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
2701 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
2761 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
2821 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
2881 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
2941 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
3001 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
3061 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
3121 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
3181 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
3241 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
3301 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
3361 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc
3421 cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct
3481 gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg
3541 cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
3601 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
3661 catcaccgaa acgcgcgagg cagcagatca attcgcgcgc gaaggcgaag cggcatgcat
3721 aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac cctatgctac tccgtcaagc
3781 cgtcaattgt ctgattcgtt accaattatg acaacttgac ggctacatca ttcacttttt
3841 cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta aatacccgcg
3901 agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata ggcatccggg
3961 tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag cttaagacgc
4021 taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag caaacatgct
4081 gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag
4141 cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc
4201 gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc ccttcccctt
4261 gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg
4321 ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca tgccagtagg
4381 cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga tgacgaccgt
4441 agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa acaaattctc
4501 gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata taacctttca
4561 ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac
4621 ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt tgcgcttcag
4681 ccatactttt catactcccg ccattcagag
//
