| 质粒名称: | pLYL225 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002796 |
| 产品用途: | Mammalian constitutive expression vector for ecDHFRY100I-mediated Type-N TMP-inducible ssHwTx secretion (PhEF1α-SP-ecDHFRY100I-FCS-ssHwTx-pA). |
| 存储实验室: | Mingqi XIE Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | hEF1a |
| 拷贝数: | 未拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | ecDHFRY100I/ssHwTx |
| 物种来源: | synthetic |
| 密码子优化: | 未优化 |
pLYL225
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pLYL225载体质粒基本信息
pLYL225质粒图谱载体图谱和pLYL225载体序列质粒序列多克隆位点信息
pLYL225质粒载体简介
pLYL225质粒序列载体序列
LOCUS pLYL225 7136 bp DNA circular SYN 15-JUL-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7136)
AUTHORS Westlake University
TITLE Direct Submission
JOURNAL Exported Sep 12, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7136
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 147..189
/label=olyl254
primer_bind 167..189
/label=olyl433
misc_feature 173..1239
/label=P_EF1a
primer_bind 173..197
/label=P_EF1a F
primer_bind 173..196
/label=OGT184-F
primer_bind 1089..1109
/label=P-EF1a-SEQ-F
primer_bind complement(1210..1239)
/label=P_EF1a R
primer_bind complement(1222..1252)
/label=olyl434
primer_bind complement(1230..1271)
/label=olyl253
primer_bind 1242..1277
/label=olyl612
primer_bind 1256..1283
/label=olyl539
primer_bind 1262..1289
/label=oSCL1108
primer_bind 1262..1275
/label=olyl396
primer_bind 1266..1289
/label=oSCL084
misc_feature 1268..1336
/label=Secretory (IgK)
/label=nonstandard type: signal
primer_bind complement(1334..1374)
/label=olyl540
primer_bind complement(1334..1359)
/label=olyl500
primer_bind 1343..1370
/label=olyl339
primer_bind 1343..1370
/label=olyl345
primer_bind 1343..1370
/label=olyl347
primer_bind complement(1353..1387)
/label=olyl547
CDS 1355..1831
/codon_start=1
/label=ecDHFR(Y100I)
/translation="MISLIAALAVDHVIGMENAMPWNLPADLAWFKRNTLNKPVIMGRH
TWESIGRPLPGRKNIILSSQPSTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVIEQFL
PKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
primer_bind 1355..1378
/label=olyl541
misc_feature 1358..1360
/label=NLuc (nano Luciferase)
/label=nonstandard type: reporter
primer_bind 1371..1410
/label=olyl548
primer_bind complement(1393..1414)
/label=OGT61-mDHFR-R
primer_bind 1399..1423
/label=OGT61-mDHFR-2-F
primer_bind complement(1622..1660)
/label=OLYL551
primer_bind complement(1624..1654)
/label=olyl549
primer_bind 1636..1673
/label=olyl550
primer_bind complement(1642..1662)
/label=OGT62-mDHFR-R
primer_bind 1647..1667
/label=OGT62-mDHFR-2-F
primer_bind 1654..1681
/label=OLYL552
primer_bind complement(1807..1849)
/label=olyl542
primer_bind complement(1825..1849)
/label=OLYL595
primer_bind 1830..1878
/label=olyl613
primer_bind 1833..1878
/label=olyl560
CDS 1838..1849
/codon_start=1
/label=fcs
/translation="RIKR"
primer_bind 1844..1873
/label=Hwtx-F
misc_feature 1849..2551
sig_peptide 1849
/codon_start=1
/product="human interleukin-2 signal sequence"
/label=IL-2 signal sequence
/translation=""
misc_feature 2582..2695
/label=optimized Hwtx-IV(H)
primer_bind complement(2670..2692)
/label=Hwtx-R
primer_bind complement(2673..2692)
/label=olyl338
primer_bind complement(2680..2718)
/label=olyl614
misc_feature 2704..2705
/label=insert from pWH60
terminator 2733..2960
/label=bGH polyA
primer_bind complement(2972..2998)
/label=OGT184-R
rep_origin 3006..3434
/label=f1 bacteriophage origin of replication
/label=f1 bacteriophage origin of replication; arrow _f1
ori
misc_feature 3448..3777
/label=P_SV40
/label=nonstandard type: promoter_const
rep_origin 3628..3763
/label=SV40 ORI
misc_feature 3680..3729
/label=P_SV40 TSS-region
CDS 3844..4638
/label=Translation 2136-2930
misc_feature 3844..4635
/label=Kan/neoR
/label=nonstandard type: resistance
primer_bind complement(4978..4998)
/label=M13-rev
misc_binding complement(5004..5026)
/label=LacO
promoter complement(5030..5060)
/label=lac promoter
rep_origin complement(5384..5969)
/direction=LEFT
/label=high-copy-number ColE1/pMB1/pBR322/pUC origin o
/label=high-copy-number ColE1/pMB1/pBR322/pUC origin of
_ori
CDS complement(6140..7000)
/label=Translation 5292-4432
misc_feature complement(6143..7000)
/label=AmpR
/label=nonstandard type: resistance
promoter complement(7001..7105)
/label=AmpR promoter
primer_bind 7042..7070
/label=olyl231-amp-pro
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattggcta gcgggaaagt
181 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
241 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
301 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
361 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
421 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
481 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
541 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
601 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
661 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
721 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
781 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
841 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
901 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
961 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
1021 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
1081 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
1141 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
1201 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gaattagctt ggtacaagct
1261 tgccaccatg actagtgaga cagacacact cctgctatgg gtactgctgc tctgggttcc
1321 aggttccact ggtgacgcta gtggtggttc tggtatgatc agcctgatcg ccgccctggc
1381 cgtggaccac gtgatcggca tggagaacgc gatgccctgg aacctgcccg ccgacctggc
1441 ctggttcaag agaaacaccc tgaacaagcc cgtgatcatg ggcagacaca cctgggagag
1501 catcggcaga cccctgcccg gcagaaagaa catcatcctg agcagccagc ccagcaccga
1561 cgacagagtg acctgggtga agagcgtgga cgaggccatc gccgcctgcg gcgacgtgcc
1621 cgagatcatg gtgatcggcg gcggcagagt gatcgagcag ttcctgccca aggcccagaa
1681 gctgtacctg acccacatcg acgccgaggt ggagggcgac acccacttcc ccgactacga
1741 gcccgacgac tgggagagcg tgttcagcga gttccacgac gccgacgccc agaacagcca
1801 cagctactgc ttcgagatcc tggagagaag agctagcagg atcaagagga tatcggccat
1861 ggttagatct gacaaaactc acacatgccc accgtgccca gcacctgaac tcctgggggg
1921 accgtcagtc ttcctcttcc ccccaaaacc caaggacacc ctcatgatct cccggacccc
1981 tgaggtcaca tgcgtggtgg tggacgtgag ccacgaagac cctgaggtca agttcaactg
2041 gtacgtggac ggcgtggagg tgcataatgc caagacaaag ccgcgggagg agcagtacaa
2101 cagcacgtac cgtgtggtca gcgtcctcac cgtcctgcac caggactggc tgaatggcaa
2161 ggagtacaag tgcaaggtct ccaacaaagc cctcccagcc cccatcgaga aaaccatctc
2221 caaagccaaa gggcagcccc gagaaccaca ggtgtacacc ctgcccccat cccgggagga
2281 gatgaccaag aaccaggtca gcctgacctg cctggtcaaa ggcttctatc ccagcgacat
2341 cgccgtggag tgggagagca atgggcagcc ggagaacaac tacaagacca cgcctcccgt
2401 gctggactcc gacggctcct tcttcctcta cagcaagctc accgtggaca agagcaggtg
2461 gcagcagggg aacgtcttct catgctccgt gatgcacgag gctctgcaca accactacac
2521 gcagaagagc ctctccctgt ctccgggtaa agccagcgga agtggcggag gaggcggatc
2581 cggctgcctg ggcatcttca aggcctgcaa ccccagcaac gaccagtgct gcaagagcag
2641 caagctcgtg tgcagcagaa agacccggtg gtgcaagtgg cagatcggca agtgagattc
2701 tagagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
2761 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
2821 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
2881 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
2941 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta
3001 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
3061 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
3121 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
3181 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
3241 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
3301 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
3361 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
3421 atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
3481 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
3541 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
3601 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
3661 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc
3721 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
3781 ctcccgggag cttgtatatc cattttcgga tctgatcaag agacaggatg aggatcgttt
3841 cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta
3901 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg
3961 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa
4021 ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct
4081 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg
4141 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca
4201 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat
4261 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac
4321 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc
4381 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa
4441 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag
4501 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc
4561 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt
4621 cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca
4681 acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
4741 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
4801 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
4861 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
4921 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat
4981 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
5041 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
5101 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
5161 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
5221 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
5281 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
5341 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
5401 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
5461 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
5521 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
5581 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
5641 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
5701 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
5761 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
5821 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
5881 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tttttttgtt tgcaagcagc
5941 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
6001 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
6061 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
6121 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
6181 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
6241 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
6301 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
6361 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
6421 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
6481 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
6541 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
6601 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
6661 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
6721 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
6781 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
6841 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
6901 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
6961 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
7021 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
7081 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtc
//
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pLYL225质粒载体应用举例
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