| 质粒名称: | pLYL170 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002788 |
| 产品用途: | Mammalian PhCMV-driven constitutive expression vector for Type-C TMP-inducible NanoLuc secretion containing a C-terminal 6xHis-tag (PhCMV-NanoLuc-FCS-ecDHFRN18T, A19V-6xHis-tag-pA). |
| 存储实验室: | Mingqi XIE Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 未拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | ecDHFRN18T, A19V/6xHis-tag, NanoLuc |
| 物种来源: |
synthetic
|
| 密码子优化: | 未优化 |
pLYL170
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pLYL170载体质粒基本信息
pLYL170质粒图谱载体图谱和pLYL170载体序列质粒序列多克隆位点信息
pLYL170质粒载体简介
pLYL170质粒序列载体序列
LOCUS pLYL170 6545 bp DNA circular SYN 15-JUL-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6545)
AUTHORS Westlake University
TITLE Direct Submission
JOURNAL Exported Sep 12, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6545
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 186..207
/label=olyl419
misc_feature 235..818
/label=P_CMV
/label=nonstandard type: promoter_const
misc_feature 235..614
/label=P_CMV_enhancer
/label=nonstandard type: promoter_element
misc_feature 615..818
/label=P_CMV_IE
/label=nonstandard type: promoter_element
primer_bind 769..789
/label=oMHS-CMV-F
misc_feature 829
/label=putative transcriptional start
promoter 863..882
/label=T7 promoter
primer_bind complement(871..894)
/label=olyl418
primer_bind 880..947
/label=olyl539
primer_bind 884..908
/label=OLYL631
primer_bind 897..939
/label=olyl396
primer_bind 917..953
/label=oSCL1108
misc_feature 920..924
/label=NheI
primer_bind 930..953
/label=oSCL084
misc_feature 932..1000
/label=Secretory (IgK)
/label=nonstandard type: signal
primer_bind complement(996..1018)
/label=OLYL632
primer_bind complement(998..1024)
/label=olyl540
primer_bind complement(998..1021)
/label=olyl500
primer_bind complement(998..1018)
/label=OLYL616
primer_bind 999..1022
/label=OLYL636
primer_bind 1000..1027
/label=olyl555
misc_feature 1022..1531
/label=NLuc (nano Luciferase)
/label=nonstandard type: reporter
primer_bind 1022..1046
/label=olyl543
misc_feature 1052..1054
/label=cyt-STOP (TGG->TGA)
primer_bind complement(1513..1531)
/label=olyl544
primer_bind complement(1513..1531)
/label=olyl556
primer_bind complement(1517..1557)
/label=olyl459
primer_bind complement(1521..1537)
/label=oSCL085
primer_bind complement(1521..1537)
/label=oSCL1109
primer_bind 1537..1586
/label=olyl345
primer_bind 1538..1562
/label=olyl404
CDS 1538..1549
/codon_start=1
/label=fcs
/translation="RIKR"
primer_bind 1545..1586
/label=olyl339
primer_bind 1550..1586
/label=olyl347
CDS 1550..1564
/codon_start=1
/label=linker
/translation="GSGGG"
misc_feature 1571..2047
/label=ecDHFR(N18T/A19V)
primer_bind complement(1571..1590)
/label=olyl540
primer_bind complement(2025..2088)
/label=olyl402
CDS 2048..2065
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
primer_bind complement(2104..2127)
/label=olyl256
primer_bind complement(2108..2127)
/label=olyl544
primer_bind complement(2108..2127)
/label=olyl556
primer_bind complement(2108..2127)
/label=CD4(human tm+ct)-P6
terminator 2142..2369
/label=bGH polyA
rep_origin 2415..2843
/label=f1 bacteriophage origin of replication
/label=f1 bacteriophage origin of replication; arrow _f1
ori
misc_feature 2857..3186
/label=P_SV40
/label=nonstandard type: promoter_const
rep_origin 3037..3172
/label=SV40 ORI
misc_feature 3089..3138
/label=P_SV40 TSS-region
CDS 3253..4047
/label=Translation 2136-2930
misc_feature 3253..4044
/label=Kan/neoR
/label=nonstandard type: resistance
primer_bind complement(4387..4407)
/label=M13-rev
misc_binding complement(4413..4435)
/label=LacO
promoter complement(4439..4469)
/label=lac promoter
rep_origin complement(4793..5378)
/direction=LEFT
/label=high-copy-number ColE1/pMB1/pBR322/pUC origin o
/label=high-copy-number ColE1/pMB1/pBR322/pUC origin of
_ori
CDS complement(5549..6409)
/label=Translation 5292-4432
misc_feature complement(5552..6409)
/label=AmpR
/label=nonstandard type: resistance
promoter complement(6410..6514)
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gtttaaactt aagcttctgg ctagcgccac catgactagt gagacagaca cactcctgct
961 atgggtactg ctgctctggg ttccaggttc cactggtgac gctagtggtg gttctggtat
1021 ggtcttcaca ctcgaagatt tcgttgggga ctggcgacag acagccggct acaacctgga
1081 ccaagtcctt gaacagggag gtgtgtccag tttgtttcag aatctcgggg tgtccgtaac
1141 tccgatccaa aggattgtcc tgagcggtga aaatgggctg aagatcgaca tccatgtcat
1201 catcccgtat gaaggtctga gcggcgacca aatgggccag atcgaaaaaa tttttaaggt
1261 ggtgtaccct gtggatgatc atcactttaa ggtgatcctg cactatggca cactggtaat
1321 cgacggggtt acgccgaaca tgatcgacta tttcggacgg ccgtatgaag gcatcgccgt
1381 gttcgacggc aaaaagatca ctgtaacagg gaccctgtgg aacggcaaca aaattatcga
1441 cgagcgcctg atcaaccccg acggctccct gctgttccga gtaaccatca acggagtgac
1501 cggctggcgg ctgtgcgaac gcattctggc ggctagcagg atcaagaggg gttccggagg
1561 tggcgaattc atgatcagcc tgatcgccgc cctggccgtg gaccacgtga tcggcatgga
1621 gaccgtgatg ccctggaacc tgcccgccga cctggcctgg ttcaagagaa acaccctgaa
1681 caagcccgtg atcatgggca gacacacctg ggagagcatc ggcagacccc tgcccggcag
1741 aaagaacatc atcctgagca gccagcccag caccgacgac agagtgacct gggtgaagag
1801 cgtggacgag gccatcgccg cctgcggcga cgtgcccgag atcatggtga tcggcggcgg
1861 cagagtgtac gagcagttcc tgcccaaggc ccagaagctg tacctgaccc acatcgacgc
1921 cgaggtggag ggcgacaccc acttccccga ctacgagccc gacgactggg agagcgtgtt
1981 cagcgagttc cacgacgccg acgcccagaa cagccacagc tactgcttcg agatcctgga
2041 gagaagacac caccaccacc accactaaga attctgcaga tatccagcac agtggcggcc
2101 gctcgagtct agagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg
2161 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
2221 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
2281 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
2341 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc
2401 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
2461 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
2521 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
2581 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
2641 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
2701 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
2761 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
2821 ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag
2881 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
2941 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
3001 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
3061 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
3121 gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
3181 tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcaaga gacaggatga
3241 ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
3301 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
3361 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
3421 ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
3481 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
3541 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
3601 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
3661 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
3721 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
3781 cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc
3841 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
3901 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
3961 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
4021 tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag
4081 cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg
4141 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
4201 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca
4261 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
4321 ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg
4381 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
4441 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
4501 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
4561 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
4621 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
4681 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
4741 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
4801 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
4861 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
4921 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
4981 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
5041 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
5101 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
5161 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
5221 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
5281 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ttttttgttt
5341 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
5401 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
5461 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
5521 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
5581 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
5641 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
5701 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
5761 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
5821 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
5881 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
5941 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
6001 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
6061 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
6121 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
6181 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
6241 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
6301 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
6361 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
6421 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
6481 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
6541 acgtc
//
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