pCRI004-pYFAC-riboB-PgpdA-dLbCas12a-VPR-TtrpC

LOCUS       pCRI004-pYFAC-riboB-PgpdA-dLbCas12a-VPR-TtrpC               25019 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Episomal expression of dLbCas12a-VPR. Filamentous fungi vector with 
            AMA1 and riboB selection marker..
ACCESSION   .
VERSION     .
KEYWORDS    pCRI004-pYFAC-riboB-PgpdA-dLbCas12a-VPR-TtrpC
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 25019)
  AUTHORS   Roux I, Woodcraft C, Hu J, Wolters R, Gilchrist CLM, Chooi YH
  TITLE     CRISPR-mediated activation of biosynthetic gene clusters for 
            bioactive molecule discovery in filamentous fungi.
  JOURNAL   ACS Synth Biol. 2020 Jun 11. doi: 10.1021/acssynbio.0c00197.
  PUBMED    32526136
REFERENCE   2  (bases 1 to 25019)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..25019
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     2802..2823
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             4101..7784
                     /codon_start=1
                     /product="CRISPR-associated protein from Lachnospiraceae 
                     bacterium ND2006 (Zetsche et al., 2015)"
                     /label=LbCpf1
                     /translation="MSKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAE
                     DYKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKE
                     IAKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFDNRENMFS
                     EEAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEG
                     EFFNFVLTQEGIDVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVL
                     SDRESLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKN
                     GPAISTISKDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQ
                     LQEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKD
                     LLDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYS
                     KDKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNG
                     NYEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKL
                     IDFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLV
                     EEGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKK
                     EELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKI
                     NTEVRVLLKHDDNPYVIGIARGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTD
                     YHSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSG
                     FKNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQ
                     NGFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALD
                     YKNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQ
                     QGDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRN
                     YEAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTS
                     VKH"
     CDS             7785..7832
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             7839..7919
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             7926..7946
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7971..8120
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             8145..8165
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8595..8951
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALL"
     CDS             8970..9539
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
     CDS             complement(10361..11176)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     11084..11103
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     11168..11187
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             17103..17963
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(17321..17340)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      18134..18722
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     18623..18642
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     18876..18893
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    19010..19031
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        19046..19076
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    19084..19100
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     19089..19111
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     19108..19124
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     19108..19124
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      19141..20483
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(20104..20151)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     20524..20542
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(20580..20602)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     20702..20721
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        20745..20960
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             20961..21764
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     primer_bind     complement(22046..22063)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(22046..22062)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(22055..22077)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(22362..22381)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      22403..2632
                     /label=AMA1
                     /note="replication origin from Aspergillus nidulans 
                     (Aleksenko and Clutterbuck, 1996)"
ORIGIN
        1 tgcagatttt gacctagtta acaaggcaca tcatgctatc acttccaaca ctcttgctga
       61 gcgcgttctg ccttgccttt ctggttctac gaagtatcca gtcccgcctg caacgccgca
      121 gaaacgccaa aaggtggggt tgtcaaccgc cagctagagc tccatcggga ttctttggca
      181 ttccgccgtt cctcggtggt cgcagataac tccctaaatt ccctgagcta actctaagtc
      241 gaccatgccg tttatggtta gcgcctccca aaaaggaatg gccgacttag agttacctct
      301 tgaccgactt tttctttcct cccccttaca tttcgttacc acaacacatt cctatatcca
      361 aactccaggt acataactag tcgaaatctc ttttaaatct agtcaagaac tagatactta
      421 accttcatac tgcttagaga tagcctttga agctcttatt tcgctctttg tctcactctc
      481 accttcctcc tcctcctcca atcctttttg ctcgggacta gtccaagact agtcaacgat
      541 tagtatgccg agcattcgcg ataacgatct ccgaaagtcc ccagagtact gtcactatct
      601 cgaggcagtt aaggacgggg agcttacgct gccggatttc aagatagtaa gccgacccga
      661 ctagctttaa gcctagttac tgactagtcc acaggacgac aatggcgtgc ctgatatcca
      721 tccatatgaa gtctactgcc gagtgaaggg atgcctcaag cgtacagtga gtctactgct
      781 atactagttt ctgactagtt attaactagt tgacaggttc cctctgccaa cagaaacata
      841 ttggtcaagc acttgaagga caagaactcc cacggcatgg agtttacatt gcacaatgga
      901 cctcccacta tgaaggaact gatggaggcc aaaggcaagt cctatctaga ttacttggtg
      961 actagtccct gactagtcaa gtagcatggt atgaaggctt gtttgaaggc actgttctcc
     1021 caaccccgac tcctaccaag aagcgcaagc gagctgcgta agtttctgtg agtctaacta
     1081 gtgtattagc taatatatag cagcaccaag tccaaggacc acaatactaa gggtgtcgag
     1141 aattcgtgag tttcttctcc catttcaact agtccttgac tagtcactaa ctactttgca
     1201 gcaacgaggg tgaagctgga aatgatcagg acaatggcga gggcccgtaa gtacagccat
     1261 tcaatgcaga ctagttgcta actaatctgt gactagttca agtggtccgt acgccgtgca
     1321 tacccctgtg actggtagga atttgagcaa gcctgtcttg ccgcgcgatg agaaaggaaa
     1381 ggcaagttac attcagcccc gtacctagga tcagtgctaa tttataacct ctagccactc
     1441 tttatgcaga tccgccgtga gggtagcaag gcagctaaat cagctggtga gaaaggaacc
     1501 ataccctgca agacctgtcg caacgcaaag ggcaaaggta agctatccaa gctagtttgg
     1561 gactagattc taactagtct cagcaccgtg tggttcaaag ccatattgcg agttttggcg
     1621 ctttttctca tcgattgacg aggcaaaggg agcgagtatg caacctcgta agtcagactc
     1681 agacaaagac caactagtta ttgaccagtc ttctagaagg ctctgttgtg gatcttgagg
     1741 ccctggagag ttcctccaac aatccggaga caagcaagtc ctcgtcggac tagtcactaa
     1801 ctagactcta actagttgca gacatggata atgcaaaaga gacaagcaat gaagaaagtg
     1861 gtaagacatt tctcctttgt ggttctggac tagtctttga ctagtcacag tcttaaacaa
     1921 ggaaaatgag catgaaaatg aggaggaaaa ggctgctgag cccgaggaag tgcagggtga
     1981 tggcagacat ggtaggttaa taccttgtta gttattgcta gtcactgact agtcaataac
     2041 tagtctctga acaccttgca atcactccgt ttgcgcagct gaacagtggt gaggataata
     2101 gtagtaagtt attctagctt cagagttata ggagactaga tactaactag tattagttgc
     2161 aactaacctg gatctcagag actttggcct caatctagaa tctatctagt tgtcaactag
     2221 actgtggtat cattgtcttt tattttccta gtcctggaac tagcttctaa ctagtctccc
     2281 taatatgtgg ctgtcttgtt tttttttttt gtttccctac ccggatatct agtccccttc
     2341 taggttctgt taacctctcg ggctctgatt tagtttaacg caaacctgag attagtttct
     2401 aactagtctc taggttttct atccaccttt aattgtaata ataaatacaa gcaacgttta
     2461 tacgtcaaaa gcatttataa acttttaccc taaagtagct tgcttgtgtg tttagtttat
     2521 aattagtctc ttattaattt gatgtaggta agcccgccac aaatatatat ttttaacaag
     2581 ataccgtgga aaaacttcgt gctatcacaa aacagtatac aaaaaataag ctggctttcc
     2641 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     2701 cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac
     2761 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     2821 tggaacaaca ctcaactcta tctcgggcta ttcttttgat ttataaggga ttttgccgat
     2881 ttcggtctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     2941 aatggggtga cgatgagccg ctcttgcatc tttgtttgta ttatactgtc tttcttgtta
     3001 cacataatta ttctagaatg ccccaccgtt acatacggga cacagccatt tacatatgca
     3061 tgtggattac gagctaacga gttcattcaa atctcagaac tatcacataa tcatcattcc
     3121 cctatcgtca aagaccgtaa gacaaatccg gttcatgcac tgaacccatt cgggtagtga
     3181 gtcatttact cagcacactc gcgctgacgc tcgtcgaaca ccttcaatgc ctcctcggca
     3241 gccttgacac cactgagaac catggcaccg aaggtagggc ccatgcggtt aaagccatca
     3301 atttcagaca gctccatacc gccgattatc aagcccttag taacctcgcg ggtgttcttg
     3361 acgatggcat cctgccatgc ggagagacgg acggacgcag agagaagggc tgagtaataa
     3421 gcgccactgc gccagacagc tctggcggct ctgaggtgca gtggatgatt attaatccgg
     3481 gaccggccgc ccctccgccc cgaagtggaa aggctggtgt gcccctcgtt gaccaagaat
     3541 ctattgcatc atcggagaat atggagcttc atcgaatcac cggcagtaag cgaaggagaa
     3601 tgtgaagcca ggggtgtata gccgtcggcg aaatagcatg ccattaacct aggtacagaa
     3661 gtccaattgc ttccgatctg gtaaaagatt cacgagatag taccttctcc gaagtaggta
     3721 gagcgagtac ccggcgcgta agctccctaa ttggcccatc cggcatctgt agggcgtcca
     3781 aatatcgtgc ctctcctgct ttgcccggtg tatgaaaccg gaaaggccgc tcaggagctg
     3841 gccagcggcg cagaccggga acacaagctg gcagtcgacc catccggtgc tctgcactcg
     3901 acctgctgag gtccctcagt ccctggtagg cagctttgcc ccgtctgtcc gcccggtgtg
     3961 tcggcggggt tgacaaggtc gttgcgtcag tccaacattt gttgccatat tttcctgctc
     4021 tccccaccag ctgctctttt cttttctctt tcttttccca tcttcagtat attcatcttc
     4081 ccatccaaga acctttaatc atgagcaagc tggagaagtt tacaaactgc tactccctgt
     4141 ctaagaccct gaggttcaag gccatccctg tgggcaagac ccaggagaac atcgacaata
     4201 agcggctgct ggtggaggac gagaagagag ccgaggatta taagggcgtg aagaagctgc
     4261 tggatcgcta ctatctgtct tttatcaacg acgtgctgca cagcatcaag ctgaagaatc
     4321 tgaacaatta catcagcctg ttccggaaga aaaccagaac cgagaaggag aataaggagc
     4381 tggagaacct ggagatcaat ctgcggaagg agatcgccaa ggccttcaag ggcaacgagg
     4441 gctacaagtc cctgtttaag aaggatatca tcgagacaat cctgccagag ttcctggacg
     4501 ataaggacga gatcgccctg gtgaacagct tcaatggctt taccacagcc ttcaccggct
     4561 tctttgataa cagagagaat atgttttccg aggaggccaa gagcacatcc atcgccttca
     4621 ggtgtatcaa cgagaatctg acccgctaca tctctaatat ggacatcttc gagaaggtgg
     4681 acgccatctt tgataagcac gaggtgcagg agatcaagga gaagatcctg aacagcgact
     4741 atgatgtgga ggatttcttt gagggcgagt tctttaactt tgtgctgaca caggagggca
     4801 tcgacgtgta taacgccatc atcggcggct tcgtgaccga gagcggcgag aagatcaagg
     4861 gcctgaacga gtacatcaac ctgtataatc agaaaaccaa gcagaagctg cctaagttta
     4921 agccactgta taagcaggtg ctgagcgatc gggagtctct gagcttctac ggcgagggct
     4981 atacatccga tgaggaggtg ctggaggtgt ttagaaacac cctgaacaag aacagcgaga
     5041 tcttcagctc catcaagaag ctggagaagc tgttcaagaa ttttgacgag tactctagcg
     5101 ccggcatctt tgtgaagaac ggccccgcca tcagcacaat ctccaaggat atcttcggcg
     5161 agtggaacgt gatccgggac aagtggaatg ccgagtatga cgatatccac ctgaagaaga
     5221 aggccgtggt gaccgagaag tacgaggacg atcggagaaa gtccttcaag aagatcggct
     5281 ccttttctct ggagcagctg caggagtacg ccgacgccga tctgtctgtg gtggagaagc
     5341 tgaaggagat catcatccag aaggtggatg agatctacaa ggtgtatggc tcctctgaga
     5401 agctgttcga cgccgatttt gtgctggaga agagcctgaa gaagaacgac gccgtggtgg
     5461 ccatcatgaa ggacctgctg gattctgtga agagcttcga gaattacatc aaggccttct
     5521 ttggcgaggg caaggagaca aacagggacg agtccttcta tggcgatttt gtgctggcct
     5581 acgacatcct gctgaaggtg gaccacatct acgatgccat ccgcaattat gtgacccaga
     5641 agccctactc taaggataag ttcaagctgt attttcagaa ccctcagttc atgggcggct
     5701 gggacaagga taaggagaca gactatcggg ccaccatcct gagatacggc tccaagtact
     5761 atctggccat catggataag aagtacgcca agtgcctgca gaagatcgac aaggacgatg
     5821 tgaacggcaa ttacgagaag atcaactata agctgctgcc cggccctaat aagatgctgc
     5881 caaaggtgtt cttttctaag aagtggatgg cctactataa ccccagcgag gacatccaga
     5941 agatctacaa gaatggcaca ttcaagaagg gcgatatgtt taacctgaat gactgtcaca
     6001 agctgatcga cttctttaag gatagcatct cccggtatcc aaagtggtcc aatgcctacg
     6061 atttcaactt ttctgagaca gagaagtata aggacatcgc cggcttttac agagaggtgg
     6121 aggagcaggg ctataaggtg agcttcgagt ctgccagcaa gaaggaggtg gataagctgg
     6181 tggaggaggg caagctgtat atgttccaga tctataacaa ggacttttcc gataagtctc
     6241 acggcacacc caatctgcac accatgtact tcaagctgct gtttgacgag aacaatcacg
     6301 gacagatcag gctgagcgga ggagcagagc tgttcatgag gcgcgcctcc ctgaagaagg
     6361 aggagctggt ggtgcaccca gccaactccc ctatcgccaa caagaatcca gataatccca
     6421 agaaaaccac aaccctgtcc tacgacgtgt ataaggataa gaggttttct gaggaccagt
     6481 acgagctgca catcccaatc gccatcaata agtgccccaa gaacatcttc aagatcaata
     6541 cagaggtgcg cgtgctgctg aagcacgacg ataaccccta tgtgatcggc atcgctcgag
     6601 gcgagcgcaa tctgctgtat atcgtggtgg tggacggcaa gggcaacatc gtggagcagt
     6661 attccctgaa cgagatcatc aacaacttca acggcatcag gatcaagaca gattaccact
     6721 ctctgctgga caagaaggag aaggagaggt tcgaggcccg ccagaactgg acctccatcg
     6781 agaatatcaa ggagctgaag gccggctata tctctcaggt ggtgcacaag atctgcgagc
     6841 tggtggagaa gtacgatgcc gtgatcgccc tggaggacct gaactctggc tttaagaata
     6901 gccgcgtgaa ggtggagaag caggtgtatc agaagttcga gaagatgctg atcgataagc
     6961 tgaactacat ggtggacaag aagtctaatc cttgtgcaac aggcggcgcc ctgaagggct
     7021 atcagatcac caataagttc gagagcttta agtccatgtc tacccagaac ggcttcatct
     7081 tttacatccc tgcctggctg acatccaaga tcgatccatc taccggcttt gtgaacctgc
     7141 tgaaaaccaa gtataccagc atcgccgatt ccaagaagtt catcagctcc tttgacagga
     7201 tcatgtacgt gcccgaggag gatctgttcg agtttgccct ggactataag aacttctctc
     7261 gcacagacgc cgattacatc aagaagtgga agctgtactc ctacggcaac cggatcagaa
     7321 tcttccggaa tcctaagaag aacaacgtgt tcgactggga ggaggtgtgc ctgaccagcg
     7381 cctataagga gctgttcaac aagtacggca tcaattatca gcagggcgat atcagagccc
     7441 tgctgtgcga gcagtccgac aaggccttct actctagctt tatggccctg atgagcctga
     7501 tgctgcagat gcggaacagc atcacaggcc gcaccgacgt ggattttctg atcagccctg
     7561 tgaagaactc cgacggcatc ttctacgata gccggaacta tgaggcccag gagaatgcca
     7621 tcctgccaaa gaacgccgac gccaatggcg cctataacat cgccagaaag gtgctgtggg
     7681 ccatcggcca gttcaagaag gccgaggacg agaagctgga taaggtgaag atcgccatct
     7741 ctaacaagga gtggctggag tacgcccaga ccagcgtgaa gcacaaaagg ccggcggcca
     7801 cgaaaaaggc cggccaggca aaaaagaaaa agggatccta cccatacgat gttccagatt
     7861 acgcttatcc ctacgacgtg cctgattatg catacccata tgatgtcccc gactatgccg
     7921 ctgaccccaa gaagaagagg aaggtggagg ccagcggttc cggacgggct gacgcattgg
     7981 acgattttga tctggatatg ctgggaagtg acgccctcga tgattttgac cttgacatgc
     8041 ttggttcgga tgcccttgat gactttgacc tcgacatgct cggcagtgac gcccttgatg
     8101 atttcgacct ggacatgctg attaactcta gaagttccgg atctccgaaa aagaaacgca
     8161 aagttggtag ccagtacctg cccgacaccg acgaccggca ccggatcgag gaaaagcgga
     8221 agcggaccta cgagacattc aagagcatca tgaagaagtc ccccttcagc ggccccaccg
     8281 accctagacc tccacctaga agaatcgccg tgcccagcag atccagcgcc agcgtgccaa
     8341 aacctgcccc ccagccttac cccttcacca gcagcctgag caccatcaac tacgacgagt
     8401 tccctaccat ggtgttcccc agcggccaga tctctcaggc ctctgctctg gctccagccc
     8461 ctcctcaggt gctgcctcag gctcctgctc ctgcaccagc tccagccatg gtgtctgcac
     8521 tggctcaggc accagcaccc gtgcctgtgc tggctcctgg acctccacag gctgtggctc
     8581 caccagcccc taaacctaca caggccggcg agggcacact gtctgaagct ctgctgcagc
     8641 tgcagttcga cgacgaggat ctgggagccc tgctgggaaa cagcaccgat cctgccgtgt
     8701 tcaccgacct ggccagcgtg gacaacagcg agttccagca gctgctgaac cagggcatcc
     8761 ctgtggcccc tcacaccacc gagcccatgc tgatggaata ccccgaggcc atcacccggc
     8821 tcgtgacagg cgctcagagg cctcctgatc cagctcctgc ccctctggga gcaccaggcc
     8881 tgcctaatgg actgctgtct ggcgacgagg acttcagctc tatcgccgac atggacttct
     8941 ccgcactgct gggtagcgga tcgggatctc gggattccag ggaagggatg tttttgccga
     9001 agcctgaggc cggctccgct attagtgacg tgtttgaggg ccgcgaggtg tgccagccaa
     9061 aacgaatccg gccatttcat cctccaggaa gtccatgggc caaccgccca ctccccgcca
     9121 gcctcgcacc aacaccaacc ggtccagtac atgagccagt cgggtcactg accccggcac
     9181 cagtccctca gccactggat ccagcgcccg cagtgactcc cgaggccagt cacctgttgg
     9241 aggatcccga tgaagagacg agccaggctg tcaaagccct tcgggagatg gccgatactg
     9301 tgattcccca gaaggaagag gctgcaatct gtggccaaat ggacctttcc catccgcccc
     9361 caaggggcca tctggatgag ctgacaacca cacttgagtc catgaccgag gatctgaacc
     9421 tggactcacc cctgaccccg gaattgaacg agattctgga taccttcctg aacgacgagt
     9481 gcctcttgca tgccatgcat atcagcacag gactgtccat cttcgacaca tctctgtttt
     9541 agtgggcgac gacgacgttc gagggggggc ccggtaccgc cccgtccggt cctgcccgtc
     9601 accgagatcc acttaacgtt actgaaatca tcaaacagct tgacgaatct ggatataaga
     9661 tcgttggtgt cgatgtcagc tccggagttg agacaaatgg tgttcaggat ctcgataaga
     9721 tacgttcatt tgtccaagca gcaaagagtg ccttctagtg atttaatagc tccatgtcaa
     9781 caagaataaa acgcgtttcg ggtttacctc ttccagatac agctcatctg caatgcatgg
     9841 ccgccctgaa tacggcctga attgattgta gacctttaga ctaaatcttc tttggctgcg
     9901 ctaagtggca gagacttcac aggtgtgagt cgagggtata cgcaccatac agaggcacgg
     9961 ctggatgaca attaatggag atagaagtca tgatagacag cgggctgact tttcaattga
    10021 cagaatctga gtgtgcatat agtaggacca aagaaaccgc ctaaatccac ttattcagag
    10081 tattcctctt cgatctggag agcatacaat gtaggtgata cttcatggca tcatcccgca
    10141 cgactctctc gcgggctcgc gctccacggg gactcgcttc aatttgttcc gcggcctatt
    10201 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc
    10261 ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
    10321 ctaaatacat tcaaatatgt atccgctcat gaattaattc ttagaaaaac tcatcgagca
    10381 tcaaatgaaa ctgcaattta ttcatatcag gattatcaat accatatttt tgaaaaagcc
    10441 gtttctgtaa tgaaggagaa aactcaccga ggcagttcca taggatggca agatcctggt
    10501 atcggtctgc gattccgact cgtccaacat caatacaacc tattaatttc ccctcgtcaa
    10561 aaataaggtt atcaagtgag aaatcaccat gagtgacgac tgaatccggt gagaatggca
    10621 aaagtttatg catttctttc cagacttgtt caacaggcca gccattacgc tcgtcatcaa
    10681 aatcactcgc atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg agacgaaata
    10741 ccctgtctga gttaaaagga caattacaaa caggaatcga atgcaaccgg cgcaggaaca
    10801 ctgccagcgc atcaacaata ttttcacctg aatcaggata ttcttctaat acctggaatg
    10861 ctgttttccc ggggatcgca gtggtgagta accatgcatc atcaggagta cggataaaat
    10921 gcttgatggt cggaagaggc ataaattccg tcagccagtt tagtctgacc atctcatctg
    10981 taacatcatt ggcaacgcta cctttgccat gtttcagaaa caactctggc gcatcgggct
    11041 tcccatacaa tcgatagatt gtcgcacctg attgcccgac attatcgcga gcccatttat
    11101 acccatataa atcagcatcc atgttggaat ttaatcgcgg cctagagcaa gacgtttccc
    11161 gttgaatatg gctcataaca ccccttgtat tactgtttat gtaagcagac agttttattg
    11221 ttcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
    11281 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
    11341 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
    11401 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagtcg
    11461 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
    11521 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
    11581 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggtttgtg cacacagccc
    11641 agcttggagc gaacgaccta caccgaactg agatactaac cattattttt ttcctcaaca
    11701 taacgagaac acacaggggc gctatcgcac agaatcaaat tcgatgactg gaaatttttt
    11761 gttaatttca gaggtcgcct gacgcatata cctttttcaa ctgaaaaatt gggagaaaaa
    11821 ggaaaggtga gagcgccgga accggctttt catatagaat agagaagcgt tcatgactaa
    11881 atgcttgcat cacaatactt gaagttgaca atattattta aggacctatt gtttttccaa
    11941 taggtggtta gcaatcgtct tactttctaa cttttcttac cttttacatt tcagcaatat
    12001 atatatatat ttcaaggata taccattcta atgtctgccc ctaagaagat cgtcgttttg
    12061 ccaggtgacc acgttggtca agaaatcaca gccgaagcca ttaaggttct taaagctatt
    12121 tctgatgttc gttccaatgt caagttcgat ttcgaaaatc atttaattgg tggtgctgct
    12181 atcgaagcta caggtgtccc acttccagat gaggcgctgg aagcctccaa gaaggctgat
    12241 gccgttttgt taggtgctgt gggtggtcct aaatggggta caggtagtgt tagacctgaa
    12301 caaggtttac taaaaatccg taaagaactt caattgtacg ccaacttaag accatgtaac
    12361 tttgcatccg actctctttt agacttatct ccaatcaagc cacaatttgc taaaggtact
    12421 gacttcgttg ttgtcagaga attagtggga ggtatttact ttggtaagag aaaggaagat
    12481 gatggtgatg gtgtcgcttg ggatagtgaa caatacaccg ttccagaagt gcaaagaatc
    12541 acaagaatgg ccgctttcat ggccctacaa catgagccac cattgcctat ttggtccttg
    12601 gataaagcta atgttttggc ctcttcaaga ttatggagaa aaactgtgga ggaaaccatc
    12661 aagaacgaat ttcctacatt gaaggttcaa catcaattga ttgattctgc cgccatgatc
    12721 ctagttaaga acccaaccca cctaaatggt attataatca ccagcaacat gtttggtgat
    12781 atcatctccg atgaagcctc cgttatccca ggttccttgg gtttgttgcc atctgcgtcc
    12841 ttggcctctt tgccagacaa gaacaccgca tttggtttgt acgaaccatg ccacggttct
    12901 gctccagatt tgccaaagaa taaggtcaac cctatcgcca ctatcttgtc tgctgcaatg
    12961 atgttgaaat tgtcattgaa cttgcctgaa gaaggtaagg ccattgaaga tgcagttaaa
    13021 aaggttttgg atgcaggtat cagaactggt gatttaggtg gttccaacag taccaccgaa
    13081 gtcggtgatg ctgtcgccga agaagttaag aaaatcctca cccctgcatc ttggaatagc
    13141 ttgcagtagg tctaacagtg ctagagctgt actattgtaa gttgaaacct tggatacaac
    13201 cttggctcga agagatgcca aaacgatcct ttctgctact gatgaatcac cggcatagag
    13261 tattatatgt gcataaatat tgagcagagc tgtggcaagt ggccttggct ggaatccgac
    13321 taaaaaaata acacgcaaaa aaaaggaaga cgggtgaggg gctgtttgag ccttgattca
    13381 aagtcaaatc atcgcatttg gctaccataa tacctatact tcccagtcta tccagtctgt
    13441 ggtttatgat tgcttacaag tgctatccgt atcaggctag gaaaagtgga agggaaatcg
    13501 gttgaaccaa ggccaaaatc attgtaccat cttgattgaa aactatatac aaagatatca
    13561 agcatggaca tcataaacat gaaacatctg acgattgtgt ttgtagttcc agcccgtggc
    13621 cgttgaaacg cttattctgg catcttgcgg ccgctggatg actccctttc gatgataccc
    13681 tttagcagaa gtgttcttaa attgcgggaa gaggccgttc aggagtctgg ctcgtttgat
    13741 cacatgggat taaaatctgg tgttgaggct gggatatttt atgttttgtc tcttgcgctt
    13801 cgatatcaac agattgacaa taatgaataa ctatctacgg actatgaatg cagctcgatc
    13861 tactccgtat ttagtcgggc aatctcccgt agaagatgct agactgcgct gaaagactag
    13921 gcacagttaa ttgccgcgga tcacgtcagg cctgacaaat gacaggtgga ccttcatgta
    13981 atcgcacgat gagtcagcgc ctcttctccc ccgccgactt gcagctgcgg atgtcccgag
    14041 gagccgaaca ttgaatctat cccaagagtg actgaaatat gattgcaatc gtcgtctttt
    14101 tcttgcagag aatgtggtga ggtgatcaat cgtgtttttc ttcatatatt atattctgta
    14161 atgatcatac tttgttgaga tacccgtctc taaaattgta ctttcgcctc cctcctccct
    14221 ctcaagtccg tcatcgcaat gtcgaattta ccgtcccatg cttctccgtc ttttggggcg
    14281 caaacgccta ccacgccgca gcccgtcgac gagctttctc tcaagtcgaa catcacatca
    14341 gcccctgcgt tacactccag attagacgaa ctccgagatg agacttccag ccagatcagc
    14401 agcatagaca ctgaccgcgg ggacatgacc cctgcggttc ccgcatcatt actctcccca
    14461 tccttcactc ctccagcaac ccccgggggc tcgatcaata ccgcggagct gcttcaacag
    14521 acccaattag ctggcgcacg gcaagggtca acacatacta agcccccgag gttgctttct
    14581 cgtcttccca atgtcgaatg tatcgtccgc gctcggatac ccacgacaac tggcgcggag
    14641 atgtttcttc acctgtacca caatgactta gacaacaaag agcatttggc gattgtgttt
    14701 ggaaatacaa ttcgaagcag aagcttggac aagatccggc ctggagagac ggagatggat
    14761 cgcatgatcc gtggcgcgta tgtcgggaaa ctccgaccgg gccgtgtgag cagctggtat
    14821 gacgaggaga aagctgaaga ggccgccagt ccggaagctg gcggcgctgt atccagtccg
    14881 cacacactgc cgcgaagtag cctcaaggag gcacctcttg tgagaatcca ttcggagtgc
    14941 tacactggtg agacggcttg gtctgcacgc tgcgactgcg gcgagcagct cgatgaagca
    15001 gctcgcctca tgtcgctgcc tatggaaacg ttggcagagg ctgcgtcacc gccggatggg
    15061 gcggtaccgt caaatgctgc tggaggtgta attgtgtacc tgcgccaaga aggacgaggc
    15121 attgggctgg gcgagaaact gaaagcgtac aatcttcagg atctgggatc agacacggtg
    15181 gaagccaatc tcctgcttcg ccaccctgcg gatgcaagaa gttatggatt agctacggcg
    15241 atcctggtgg atcttggtct agggattgac tcgaatccgc acggcatccg actactcacg
    15301 aataacccgg acaagattcg agcggttgag ggacccaacc gagaagtggt ggtgaaagag
    15361 cgggtgccga tggtgccatt ggcatggaga tcgggtggaa agatgggaat caagagttcc
    15421 gaggtcgaag ggtatctgag aacgaaggta tgtcgacaat ctatctcttg caagctagct
    15481 tattttctca tcattcttga acaatgtctc actctttcct cgcagatatc gaaaatgggc
    15541 catttgctcc aatgaaccta tcccctttcg gttagtaacc ttgtgctgga agtacgcctt
    15601 cctgcttttg gacttagcca agtcgacacg gtgtatgctc gtcacactca tgtaacggtt
    15661 ctgcagcgca aacgttctgg tcacgttatt cgcttttgtc cattttgtct ttttgtgcgg
    15721 tacataaact agaggattag cgcactttgt acatgatgtt cccgacatgt cacattaggc
    15781 ctcgattggt caaaataatc cctctctaac ctctggttcg catactctta aatgcagctc
    15841 tagtctctag acggactagt tcctcctgca gtggatgtct gacgagtgtc tgcaaggacc
    15901 gttttccgtg tccagttagt tccaatcacc cggatatcgg cgagacgtcg gccgattgag
    15961 atcactgagt caatggcgtt gagtggcaat taatatatct ggatgcctag aatcctataa
    16021 attgggaaaa ctagagaccc cggtctatcg tggtggcagt gcgtcatttt ttcctcttgg
    16081 agcaaaagtg tagtgccagt acgagtgttg tggaggaagg ctgcatacat tgtgcctgtc
    16141 attaaacgat gagctcgtcc gtattggccc ctgtaatgcc atgttttccg cccccaatcg
    16201 tcaaggtttt ccctttgtta gattcctacc agtcatctag caagtgaggt aagctttgcc
    16261 agaaacgcca aggctttatc tatgtagtcg ataagcaaag tggactgata gcttaatatg
    16321 gaaggtccct caggacaagt cgacctgtgc agaagagata acagcttggc atcacgcatc
    16381 agtgcctcct ctcagacaga ataagtcgcc ataagttatc gaccgaacct aggtagggta
    16441 tatttggtga cgacaatacc tcccgacgat acctggatta gacaccgtat gccgcgaatt
    16501 aacgccttcc gaccaagtcc cttttggtga tcgacacttt aggatcatct cctgccagtc
    16561 gcccagactg acggtgttat ggtggcgcgc tatggctttc ctagccggtc aagctccgtg
    16621 cactagagag cggagaagga gggggagttg atagggaatg atatctaggt agagaagtac
    16681 tccaaccaga gatatgcgga tcaatgctcc ggcagttgtt agcatacccc aacctcgttt
    16741 aagtatcctc ttcttccatc catctttatc attatttgtc tatgtgcatc cctagatcac
    16801 gtcaattatt tcgataaatg gatacgtgca ctcgggaatg cgtgattggc tcgtctcgtg
    16861 ttctcagttc ctcattcccc cccacgttac attgacaacg gagtattagc ctatggtcct
    16921 gttcgataag cttgctccat cgcctccact acgtcaagcg gagtatcaga catgagctct
    16981 accccagata tcgcctgctt atcgctggtt gctttgtcat gcaagtacag atcacatcgg
    17041 caattctgat aacagatggt gccattgtcc cgtaatcaat tgcccattga aaaaggaaga
    17101 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
    17161 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
    17221 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
    17281 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
    17341 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
    17401 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
    17461 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
    17521 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
    17581 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
    17641 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
    17701 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
    17761 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
    17821 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
    17881 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
    17941 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
    18001 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
    18061 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
    18121 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
    18181 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
    18241 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
    18301 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
    18361 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
    18421 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
    18481 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
    18541 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
    18601 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
    18661 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
    18721 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
    18781 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
    18841 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
    18901 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
    18961 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
    19021 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
    19081 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccactg
    19141 aacgaagcat ctgtgcttca ttttgtagaa caaaaatgca acgcgagagc gctaattttt
    19201 caaacaaaga atctgagctg catttttaca gaacagaaat gcaacgcgaa agcgctattt
    19261 taccaacgaa gaatctgtgc ttcatttttg taaaacaaaa atgcaacgcg agagcgctaa
    19321 tttttcaaac aaataatctg agctgcattt ttacagaaca gaaatgcaac gcgagagcgc
    19381 tattttacca acaaagaatc tatacttctt ttttgttcta caaaaatgca tcccgagagc
    19441 gctatttttc taacaaagca tcttagatta ctttttttct cctttgtgcg ctctataatg
    19501 cagtctcttg ataacttttt gcactgtagg tccgttaagg ttagaagaag gctactttgg
    19561 tgtctatttt ctcttccata aaaaaagcct gactccactt cccgcgttta ctgattacta
    19621 gcgaagctgc gggtgcattt tttcaagata aaggcatccc cgattatatt ctataccgat
    19681 gtggattgcg catactttgt gaacagaaag tgatagcgtt gatgattctt cattggtcag
    19741 aaaattatga acggtttctt ctattttgtc tctatatact acgtatagga aatgtttaca
    19801 ttttcgtatt gttttcgatt cactctatga atagttctta ctacaatttt tttgtctaaa
    19861 gagtaatact agagataaac ataaaaaatg tagaggtcga gtttagatgc aagttcaagg
    19921 agcgaaaggt ggatgggtag gttatatagg gatatagcac agagatatat agcaaagaga
    19981 tacttttgag caatgtttgt ggaagcggta ttcgcaatat tttagtagct cgttacagtc
    20041 cggtgcgttt ttggtttttt gaaagtgcgt cttcagagcg cttttggttt tcaaaagcgc
    20101 tctgaagttc ctatactttc tagagaatag gaacttcgga ataggaactt caaagcgttt
    20161 ccgaaaacga gcgcttccga aaatgcaacg cgagctgcgc acatacagct cactgttcac
    20221 gtcgcaccta tatctgcgtg ttgcctgtat atatatatac atgagaagaa cggcatagtg
    20281 cgtgtttatg cttaaatgcg tacttatatg cgtctattta tgtaggatga aaggtagtct
    20341 agtacctcct gtgatattat cccattccat gcggggtatc gtatgcttcc ttcagcacta
    20401 ccctttagct gttctatatg ctgccactcc tcaattggat tagtctcatc cttcaatgct
    20461 atcatttcct ttgatattgg atcatactaa gaaaccatta ttatcatgac attaacctat
    20521 aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac
    20581 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
    20641 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat
    20701 gcggcatcag agcagattgt actgagagtg caccatacca ccttttcaat tcatcatttt
    20761 ttttttattc ttttttttga tttcggtttc cttgaaattt ttttgattcg gtaatctccg
    20821 aacagaagga agaacgaagg aaggagcaca gacttagatt ggtatatata cgcatatgta
    20881 gtgttgaaga aacatgaaat tgcccagtat tcttaaccca actgcacaga acaaaaacct
    20941 gcaggaaacg aagataaatc atgtcgaaag ctacatataa ggaacgtgct gctactcatc
    21001 ctagtcctgt tgctgccaag ctatttaata tcatgcacga aaagcaaaca aacttgtgtg
    21061 cttcattgga tgttcgtacc accaaggaat tactggagtt agttgaagca ttaggtccca
    21121 aaatttgttt actaaaaaca catgtggata tcttgactga tttttccatg gagggcacag
    21181 ttaagccgct aaaggcatta tccgccaagt acaatttttt actcttcgaa gacagaaaat
    21241 ttgctgacat tggtaataca gtcaaattgc agtactctgc gggtgtatac agaatagcag
    21301 aatgggcaga cattacgaat gcacacggtg tggtgggccc aggtattgtt agcggtttga
    21361 agcaggcggc agaagaagta acaaaggaac ctagaggcct tttgatgtta gcagaattgt
    21421 catgcaaggg ctccctatct actggagaat atactaaggg tactgttgac attgcgaaga
    21481 gcgacaaaga ttttgttatc ggctttattg ctcaaagaga catgggtgga agagatgaag
    21541 gttacgattg gttgattatg acacccggtg tgggtttaga tgacaaggga gacgcattgg
    21601 gtcaacagta tagaaccgtg gatgatgtgg tctctacagg atctgacatt attattgttg
    21661 gaagaggact atttgcaaag ggaagggatg ctaaggtaga gggtgaacgt tacagaaaag
    21721 caggctggga agcatatttg agaagatgcg gccagcaaaa ctaaaaaact gtattataag
    21781 taaatgcatg tatactaaac tcacaaatta gagcttcaat ttaattatat cagttattac
    21841 cctatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggaaattg
    21901 taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta
    21961 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
    22021 tgagtggggt accgagctcg aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa
    22081 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
    22141 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
    22201 ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgtc
    22261 aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
    22321 gcgcagcgtg accgctacac ttgccagcgc cttagcgccc gctcctttcg ctttcttccc
    22381 ttcctttctc gccacgttcg ccagcttatt ttttgtatac tgttttgtga tagcacgaag
    22441 tttttccacg gtatcttgtt aaaaatatat atttgtggcg ggcttaccta catcaaatta
    22501 ataagagact aattataaac taaacacaca agcaagctac tttagggtaa aagtttataa
    22561 atgcttttga cgtataaacg ttgcttgtat ttattattac aattaaaggt ggatagaaaa
    22621 cctagagact agttagaaac taatctcagg tttgcgttaa actaaatcag agcccgagag
    22681 gttaacagaa cctagaaggg gactagatat ccgggtaggg aaacaaaaaa aaaaaacaag
    22741 acagccacat attagggaga ctagttagaa gctagttcca ggactaggaa aataaaagac
    22801 aatgatacca cagtctagtt gacaactaga tagattctag attgaggcca aagtctctga
    22861 gatccaggtt agttgcaact aatactagtt agtatctagt ctcctataac tctgaagcta
    22921 gaataactta ctactattat cctcaccact gttcagctgc gcaaacggag tgattgcaag
    22981 gtgttcagag actagttatt gactagtcag tgactagcaa taactaacaa ggtattaacc
    23041 taccatgtct gccatcaccc tgcacttcct cgggctcagc agccttttcc tcctcatttt
    23101 catgctcatt ttccttgttt aagactgtga ctagtcaaag actagtccag aaccacaaag
    23161 gagaaatgtc ttaccacttt cttcattgct tgtctctttt gcattatcca tgtctgcaac
    23221 tagttagagt ctagttagtg actagtccga cgaggacttg cttgtctccg gattgttgga
    23281 ggaactctcc agggcctcaa gatccacaac agagccttct agaagactgg tcaataacta
    23341 gttggtcttt gtctgagtct gacttacgag gttgcatact cgctcccttt gcctcgtcaa
    23401 tcgatgagaa aaagcgccaa aactcgcaat atggctttga accacacggt gctgagacta
    23461 gttagaatct agtcccaaac tagcttggat agcttacctt tgccctttgc gttgcgacag
    23521 gtcttgcagg gtatggttcc tttctcacca gctgatttag ctgccttgct accctcacgg
    23581 cggatctgca taaagagtgg ctagaggtta taaattagca ctgatcctag gtacggggct
    23641 gaatgtaact tgcctttcct ttctcatcgc gcggcaagac aggcttgctc aaattcctac
    23701 cagtcacagg ggtatgcacg gcgtacggac cacttgaact agtcacagat tagttagcaa
    23761 ctagtctgca ttgaatggct gtacttacgg gccctcgcca ttgtcctgat catttccagc
    23821 ttcaccctcg ttgctgcaaa gtagttagtg actagtcaag gactagttga aatgggagaa
    23881 gaaactcacg aattctcgac acccttagta ttgtggtcct tggacttggt gctgctatat
    23941 attagctaat acactagtta gactcacaga aacttacgca gctcgcttgc gcttcttggt
    24001 aggagtcggg gttgggagaa cagtgccttc aaacaagcct tcataccatg ctacttgact
    24061 agtcagggac tagtcaccaa gtaatctaga taggacttgc ctttggcctc catcagttcc
    24121 ttcatagtgg gaggtccatt gtgcaatgta aactccatgc cgtgggagtt cttgtccttc
    24181 aagtgcttga ccaatatgtt tctgttggca gagggaacct gtcaactagt taataactag
    24241 tcagaaacta gtatagcagt agactcactg tacgcttgag gcatcccttc actcggcagt
    24301 agacttcata tggatggata tcaggcacgc cattgtcgtc ctgtggacta gtcagtaact
    24361 aggcttaaag ctagtcgggt cggcttacta tcttgaaatc cggcagcgta agctccccgt
    24421 ccttaactgc ctcgagatag tgacagtact ctggggactt tcggagatcg ttatcgcgaa
    24481 tgctcggcat actaatcgtt gactagtctt ggactagtcc cgagcaaaaa ggattggagg
    24541 aggaggagga aggtgagagt gagacaaaga gcgaaataag agcttcaaag gctatctcta
    24601 agcagtatga aggttaagta tctagttctt gactagattt aaaagagatt tcgactagtt
    24661 atgtacctgg agtttggata taggaatgtg ttgtggtaac gaaatgtaag ggggaggaaa
    24721 gaaaaagtcg gtcaagaggt aactctaagt cggccattcc tttttgggag gcgctaacca
    24781 taaacggcat ggtcgactta gagttagctc agggaattta gggagttatc tgcgaccact
    24841 ggactctcag gctttccatt ggggtactaa catagccatc aaatgccggc ccgtgtttaa
    24901 tctcctcgag atggctgggc tcacatgcgt tctactcaaa aactcttata cctaggccct
    24961 ctcccaatcg tgtatgtttg gaccttcctc tctcacactt tggataacat caagcatac
//