pAAVLINKv1-pCALM1-Cre-3'-DCC-FLAG

LOCUS       pAAVLINKv1-pCALM1-Cre-3'-DCC-FLAG         7106 bp DNA     circular SYN 10-AUG-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7106)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 10, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7106
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     promoter        145..265
                     /label=pCALM1
     CDS             277..1308
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     intron          1309..1349
                     /label=Splicing Donor
     protein_bind    1356..1389
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1390..1481
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1482..3819
                     /codon_start=1
                     /gene="DCC"
                     /gene_synonym="CRC18; CRCR1; HGPPS2; IGDCC1; MRMV1;NTN1R1"
                     /product="netrin receptor DCC"
                     /label=DCC
                     /note="colorectal cancer suppressor; tumor 
                     suppressorprotein DCC; immunoglobulin superfamily DCC 
                     subclass member 1; deleted in colorectal carcinoma; 
                     putative colorectal tumor suppressor"
                     /db_xref="GI:110431348"
                     /db_xref="CCDS:CCDS11952.1"
                     /db_xref="GeneID:1630"
                     /db_xref="HGNC:HGNC:2701"
                     /db_xref="MIM:120470"
                     /protein_id="NP_005206.2"
                     /translation="GEMETLEPNNLWYLFTGLEKGSQYSFQVSAMTVNGTGPPSNWYTA
                     ETPENDLDESQVPDQPSSLHVRPQTNCIIMSWTPPLNPNIVVRGYIIGYGVGSPYAETV
                     RVDSKQRYYSIERLESSSHYVISLKAFNNAGEGVPLYESATTRSITDPTDPVDYYPLLD
                     DFPTSVPDLSTPMLPPVGVQAVALTHDAVRVSWADNSVPKNQKTSEVRLYTVRWRTSFS
                     ASAKYKSEDTTSLSYTATGLKPNTMYEFSVMVTKNRRSSTWSMTAHATTYEAAPTSAPK
                     DLTVITREGKPRAVIVSWQPPLEANGKITAYILFYTLDKNIPIDDWIMETISGDRLTHQ
                     IMDLNLDTMYYFRIQARNSKGVGPLSDPILFRTLKVEHPDKMANDQGRHGDGGYWPVDT
                     NLIDRSTLNEPPIGQMHPPHGSVTPQKNSNLLVIIVVTVGVITVLVVVIVAVICTRRSS
                     AQQRKKRATHSAGKRKGSQKDLRPPDLWIHHEEMEMKNIEKPSGTDPAGRDSPIQSCQD
                     LTPVSHSQSETQLGSKSTSHSGQDTEEAGSSMSTLERSLAARRAPRAKLMIPMDAQSNN
                     PAVVSAIPVPTLESAQYPGILPSPTCGYPHPQFTLRPVPFPTLSVDRGFGAGRSQSVSE
                     GPTTQQPPMLPPSQPEHSSSEEAPSRTIPTACVRPTHPLRSFANPLLPPPMSAIEPKVP
                     YTPLLSQPGPTLPKTHVKTASLGLAGKARSPLLPVSVPTAPEVSEESHKPTEDSANVYE
                     QDDLSEQMASLEGLMKQLNAITGSAF"
     misc_feature    3449..3454
                     /label=SDS
     CDS             3826..3891
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      3895..4326
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    4039..4044
                     /label=SDS
     repeat_region   4369..4509
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4369..4498
                     /label=AAV2 ITR
     repeat_region   4370..4510
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4584..5039
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5321..5425
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5426..6286
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6457..7045
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
      181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
      241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
      301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
      361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
      421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
      481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
      541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
      601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
      661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
      721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
      781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
      841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
      901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
      961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
     1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
     1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
     1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
     1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
     1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
     1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
     1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
     1441 tattggtctt actgacatcc actttgcctt tctctccaca ggggtgagat ggaaacactg
     1501 gagccaaaca acctctggta cctattcaca ggactggaga aaggaagtca gtacagtttc
     1561 caggtgtcag ccatgacagt caatggtact ggaccacctt ccaactggta tactgcagag
     1621 actccagaga atgatctaga tgaatctcaa gttcctgatc aaccaagctc tcttcatgtg
     1681 aggccccaga ctaactgcat catcatgagt tggactcctc ccttgaaccc aaacatcgtg
     1741 gtgcgaggtt atattatcgg ttatggcgtt gggagccctt acgctgagac agtgcgtgtg
     1801 gacagcaagc agcgatatta ttccattgag aggttagagt caagttccca ttatgtaatc
     1861 tccctaaaag cttttaacaa tgccggagaa ggagttcctc tttatgaaag tgccaccacc
     1921 aggtctataa ccgatcccac tgacccagtt gattattatc ctttgcttga tgatttcccc
     1981 acctcggtcc cagatctctc cacccccatg ctcccaccag taggtgtaca ggctgtggct
     2041 cttacccatg atgctgtgag ggtcagctgg gcagacaact ctgtccctaa gaaccaaaag
     2101 acgtctgagg tgcgacttta caccgtccgg tggagaacca gcttttctgc aagtgcaaaa
     2161 tacaagtcag aagacacaac atctctaagt tacacagcaa caggcctcaa accaaacaca
     2221 atgtatgaat tctcggtcat ggtaacaaaa aacagaaggt ccagtacttg gagcatgact
     2281 gcacatgcca ccacgtatga agcagccccc acctctgctc ccaaggactt gacagtcatt
     2341 actagggaag ggaagcctcg tgccgtcatt gtgagttggc agcctccctt ggaagccaat
     2401 gggaaaatta ctgcttacat cttattttat accttggaca agaacatccc aattgatgac
     2461 tggattatgg aaacaatcag tggtgatagg cttactcatc aaatcatgga tctcaacctt
     2521 gatactatgt attactttcg aattcaagca cgaaattcaa aaggagtggg gccactctct
     2581 gatcctatcc tcttcaggac tctgaaagtg gaacaccctg acaaaatggc taatgaccaa
     2641 ggtcgtcatg gagatggagg ttattggcca gttgatacta atttgattga tagaagcacc
     2701 ctaaatgagc cgccaattgg acaaatgcac cccccgcatg gcagtgtcac tcctcagaag
     2761 aacagcaacc tgcttgtgat cattgtggtc accgttggtg tcatcacagt gctggtagtg
     2821 gtcatcgtgg ctgtgatttg cacccgacgc tcttcagccc agcagagaaa gaaacgggcc
     2881 acccacagtg ctggcaaaag gaagggcagc cagaaggacc tccgaccccc tgatctttgg
     2941 atccatcatg aagaaatgga gatgaaaaat attgaaaagc catctggcac tgaccctgca
     3001 ggaagggact ctcccatcca aagttgccaa gacctcacac cagtcagcca cagccagtca
     3061 gaaacccaac tgggaagcaa aagcacctct cattcaggtc aagacactga ggaagcaggg
     3121 agctctatgt ccactctgga gaggtcgctg gctgcacgcc gagccccccg ggccaagctc
     3181 atgattccca tggatgccca gtccaacaat cctgctgtcg tgagcgccat cccggtgcca
     3241 acgctagaaa gtgcccagta cccaggaatc ctcccgtctc ccacctgtgg atatccccac
     3301 ccgcagttca ctctccggcc tgtgccattc ccaacactct cagtggaccg aggtttcgga
     3361 gcaggaagaa gtcagtcagt gagtgaagga ccaactaccc aacaaccacc tatgctgccc
     3421 ccatctcagc ctgagcattc tagcagcgag gaggcaccaa gcagaaccat ccccacagct
     3481 tgtgttcgac caactcaccc actccgcagc tttgctaatc ctttgctacc tccaccaatg
     3541 agtgcaatag aaccgaaagt cccttacaca ccacttttgt ctcagccagg gcccactctt
     3601 cctaagaccc atgtgaaaac agcctccctt gggttggctg gaaaagcaag atcccctttg
     3661 cttcctgtgt ctgtgccaac agcccctgaa gtgtctgagg agagccacaa accaacagag
     3721 gattcagcca atgtgtatga acaggatgat ctgagtgaac aaatggcaag tttggaagga
     3781 ctcatgaagc agcttaatgc catcacaggc tcagcctttt gtacagacta caaagaccat
     3841 gacggtgatt ataaagatca tgacatcgac tacaaggatg acgatgacaa gtaactcgag
     3901 ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     3961 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     4021 gtatggcttt cattttctcc tccttgtata aatcctggtt agttcttgcc acggcggaac
     4081 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     4141 ccgtggtgtt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc atctagcttt
     4201 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
     4261 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggagat gtgggaggtt
     4321 ttttaaagat ctggagaccg gtgtggcacg tgcggaccga gcggccgcag gaacccctag
     4381 tgatggagtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa
     4441 aggtcgcccg acgcccgggc tttgcccggg cggcctcagt gagcgagcga gcgcgcagct
     4501 gcctgcaggg gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     4561 gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     4621 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ttagcgcccg ctcctttcgc
     4681 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     4741 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgattt
     4801 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     4861 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaactctat
     4921 ctcgggctat tcttttgatt tataagggat tttgccgatt tcggtctatt ggttaaaaaa
     4981 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt
     5041 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc
     5101 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
     5161 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
     5221 cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
     5281 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     5341 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     5401 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     5461 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     5521 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     5581 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     5641 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     5701 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     5761 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     5821 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     5881 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     5941 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     6001 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     6061 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     6121 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     6181 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     6241 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     6301 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     6361 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     6421 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     6481 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     6541 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     6601 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     6661 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     6721 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     6781 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     6841 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     6901 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     6961 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     7021 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     7081 cttttgctgg ccttttgctc acatgt
//