| 出品公司: | Invitrogen |
|---|---|
| 载体名称: | pENTR3C, pENTR 3C, pENTR-3C |
| 质粒类型: | Gateway载体；入门载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 启动子: | -- |
| 克隆方法: | Gateway |
| 载体大小: | 2723bp |
| 5' 测序引物及序列: | pENTR-F: CTACAAACTCTTCCTGTTAGTTAG |
| 3' 测序引物及序列: | pENTR-R: ATGGCTCATAACACCCCTTG |
| 载体标签: | -- |
| 载体抗性: | 卡那霉素 |
| 筛选标记: | -- |
| 备注: |
载体本身含有CCDb致死基因，能够使得普通的DH5alpha等细菌死亡，必须 使用DB3.1感受态细胞进行质粒扩增，用来构建Gateway系统入门载体。 |
| 产品目录号: | 11817012 |
| 病毒/非病毒: | 非病毒 |
买家导航
pENTR3C载体 pENTR 3C质粒基本信息
pENTR3C质粒图谱 pENTR 3C载体图谱和pENTR3C载体序列 pENTR 3C质粒序列多克隆位点信息
pENTR3C质粒 pENTR 3C载体简介
pENTR3C质粒序列 pENTR 3C载体序列
LOCUS pENTR3C 2723 bp DNA circular SYN 2-SEP-2003
SOURCE
ORGANISM
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTREPLTYPE|Plasmid
COMMENT VNTEXTCHREPL|Bacteria
FEATURES Location/Qualifiers
source 1..2723
/invitrogen="563"
/vntifkey="98"
misc_recomb 358..457
/invitrogen="2670000"
/vntifkey="86"
/label=attL1
misc_recomb complement(952..1051)
/invitrogen="2680000"
/vntifkey="86"
/label=attL2
CDS 618..923
/invitrogen="1060000"
/vntifkey="4"
/label=ccdB
CDS 1174..1983
/invitrogen="1210000"
/vntifkey="4"
/label=Kan(R)
rep_origin 2047..2720
/invitrogen="2540000"
/vntifkey="33"
/label=pUC\origin
terminator complement(106..149)
/invitrogen="2580000"
/vntifkey="43"
/label=rrnB\T1\transcription\terminator
terminator complement(281..308)
/invitrogen="2590000"
/vntifkey="43"
/label=rrnB\T2\transcription\terminator
BASE COUNT 710 a 604 c 672 g 737 t
ORIGIN
1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagca tggatctcgg
61 ggacgtctaa ctactaagcg agagtaggga actgccaggc atcaaataaa acgaaaggct
121 cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
181 aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg
241 gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat
301 ggcctttttg cgtttctaca aactcttcct gttagttagt tacttaagct cgggccccaa
361 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc
421 ttttttataa tgccaacttt gtacaaaaaa gcaggctctt taaaggaacc aattcagtcg
481 actggatccg gtaccgaatt cgatcgctta ctaaaagcca gataacagta tgcgtatttg
541 cgcgctgatt tttgcggtat aagaatatat actgatatgt atacccgaag tatgtcaaaa
601 agaggtgtgc ttctagaatg cagtttaagg tttacaccta taaaagagag agccgttatc
661 gtctgtttgt ggatgtacag agtgatatta ttgacacgcc cgggcgacgg atggtgatcc
721 ccctggccag tgcacgtctg ctgtcagata aagtctcccg tgaactttac ccggtggtgc
781 atatcgggga tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg ccggtctccg
841 ttatcgggga agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta
901 acctgatgtt ctggggaata tagaattcgc ggccgcactc gagatatcta gacccagctt
961 tcttgtacaa agttggcatt ataagaaagc attgcttatc aatttgttgc aacgaacagg
1021 tcactatcag tcaaaataaa atcattattt gccatccagc tgcagctctg gcccgtgtct
1081 caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgaac aataaaactg
1141 tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtcg
1201 aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat
1261 aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg ggaagcccga tgcgccagag
1321 ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga
1381 ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct
1441 gatgatgcat ggttactcac cactgcgatc cccggaaaaa cagcattcca ggtattagaa
1501 gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg
1561 cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag
1621 gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat
1681 ggctggcctg ttgaacaagt ctggaaagaa atgcataaac ttttgccatt ctcaccggat
1741 tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta
1801 ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc
1861 ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat
1921 ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc
1981 taatcagaat tggttaattg gttgtaacat tattcagatt gggccccgtt ccactgagcg
2041 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
2101 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
2161 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
2221 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
2281 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
2341 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
2401 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
2461 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
2521 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
2581 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
2641 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
2701 tgctggcctt ttgctcacat gtt
//
