| 质粒名称: | pYK652 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000833 |
| 产品用途: | Mammalian PhCMV-driven constitutive GNCR-ZFPCDKN1C125-3xFLAG-NLS expression vector (PhCMV-GNCR-ZFPCDKN1C125-3xFLAG-NLS-pA). |
| 存储实验室: | Anping ZENG Lab |
|
载体骨架基本信息 |
|
| 载体名称: |
pCMV
|
| 表达系统: | 哺乳动物表达 |
| 启动子: |
CMV
|
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | GNCR-ZFPCDKN1C125-3xFLAG-NLS |
| 物种来源: | Synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Programmable solid-state condensates for spatiotemporal control of mammalian gene expression
Wang Y., Jiang J., Xiong Q., Li S., Shao J., Xie M., Zeng A.P.
Nat. Chem. Biol. 2025 1–10 (2025)
|
pYK652
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pYK652载体质粒基本信息
pYK652质粒图谱载体图谱和pYK652载体序列质粒序列多克隆位点信息
pYK652质粒载体简介
pYK652质粒序列载体序列
LOCUS pYK652 7017 bp DNA circular SYN 11-FEB-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7017)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 28, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7017
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 232..819
/label=CMV
primer_bind 863..882
/label=T7
primer_bind 880..946
/label=oYF0019
primer_bind 881..918
/label=oYK421_GNCR_F
primer_bind 881..902
/label=oYK31-mCherry-NS3a-F
primer_bind 910..952
/label=oYF005
primer_bind 916..937
/label=oYK481_GNCR_F
primer_bind 916..937
/label=oYK483_LCK1_F
primer_bind 916..936
/label=oYK490_P2A_GNCR1_F
CDS 919..1617
/codon_start=1
/label=GNCR1-N
/translation="DIEKLCKKAEEEAKEAQEKADELRQRHPDSQAAEDAEDLANLAVA
AVLTACLLAQEHPNADIAKLCIKAASEAAEAASKAAELAQRHPDSQAARDAIKLASQAA
RAVILAIMLAAENPNADIAKLCIKAASEAAEAASKAAELAQRHPDSQAARDAIKLASQA
AEAVERAIWLAAENPNADIAKKCIKAASEAAEEASKAAEEAQRHPDSQKARDEIKEASQ
KAEEVKERCKS"
primer_bind 919..952
/label=oYF0011
primer_bind 919..943
/label=oLZ146 GNCR1-N F
primer_bind 919..943
/label=oSCL762
primer_bind 1585..1597
/label=oYK03-02-F
primer_bind 1585..1596
/label=oYK31-mCherry-NS3a-F
primer_bind complement(1596..1653)
/label=oYF0020
primer_bind complement(1596..1647)
/label=oYF006
primer_bind complement(1596..1618)
/label=oYF0012
primer_bind complement(1605..1624)
/label=oYK465_GNCR1_R
primer_bind 1615..1637
/label=oSCL1129
primer_bind 1615..1637
/label=oSCL1192
primer_bind 1615..1635
/label=oLZ147 NS3a F
primer_bind 1615..1634
/label=oYF0033
primer_bind 1617..1637
/label=oLZ048 NSP3-F
primer_bind 1617..1634
/label=oYK419_GS_F
primer_bind 1618..1647
/label=oSCL388
primer_bind 1618..1633
/label=oYK28-mCherry-NS3a-F
primer_bind complement(1619..1667)
/label=oLZ145 DNCR2-GNCR1 R
primer_bind complement(1622..1637)
/label=oYK520_GS_R
primer_bind complement(1625..1643)
/label=Oscl18-4
primer_bind complement(1628..1648)
/label=oSCL1119
primer_bind complement(1628..1647)
/label=oSCL075
primer_bind complement(1628..1647)
/label=oSCL075a
primer_bind complement(1628..1647)
/label=oSCL075`b
primer_bind complement(1628..1647)
/label=oSCL075`c
primer_bind complement(1631..1647)
/label=oYK472_NLS_R
primer_bind 1632..1652
/label=oLZ146 GNCR1-N F
primer_bind complement(1632..1648)
/label=oYK92_L7Ae_R
primer_bind complement(1634..1662)
/label=oYK489_GNCR1_R
misc_feature 1634..1653
/label=linker
primer_bind complement(1636..1648)
/label=oYK75_NLS_mcp_R
primer_bind complement(1645..1668)
/label=oYK421_GNCR3_R
primer_bind 1649..1690
/label=oYK652_ZF125_F
misc_feature 1654..1668
/label=NSP3
CDS 1668..2376
/codon_start=1
/label=ZF_CDKN1C(125)
/translation="FACDICGRKFARKYHLSRHTRIHTGEKPFACDICGRKFADRSTLR
RHTKIHTQRPQIPPKPFACDICGRKFARKDHLRNHTRIHTGEKPFACDICGRKFAFPYL
LRRHTKIHTQRPQIPPKPFACDICGRKFALKHHLLRHTRIHTGEKPFACDICGRKFAER
SKLRRHTKIHTQRPQIPPKPFACDICGRKFALKQTLQVHTRIHTGEKPFACDICGRKFA
DRSTLRRHTKIHSR"
primer_bind 1669..1695
/label=oYK1243_CDKN1C_F
primer_bind complement(2377..2400)
/label=oYK563_ZF_R
primer_bind 2381..2404
/label=oYK563_Flag_F
CDS 2386..2451
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
primer_bind complement(2426..2489)
/label=oYK502_ZF_R
CDS 2458..2478
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
primer_bind 2480..2501
/label=oSCL1129
primer_bind 2480..2501
/label=oSCL1192
primer_bind 2480..2499
/label=oLZ147 NS3a F
primer_bind 2480..2498
/label=oYF0033
misc_feature 2482..2511
/label=linker
primer_bind 2482..2511
/label=oSCL388
misc_feature 2482..2502
/label=linker
primer_bind 2482..2501
/label=oLZ048 NSP3-F
primer_bind 2482..2498
/label=oYK419_GS_F
primer_bind 2482..2497
/label=oYK28-mCherry-NS3a-F
primer_bind complement(2483..2511)
/label=oLZ145 DNCR2-GNCR1 R
primer_bind complement(2486..2501)
/label=oYK520_GS_R
primer_bind complement(2489..2507)
/label=Oscl18-4
primer_bind complement(2492..2511)
/label=oSCL075
primer_bind complement(2492..2511)
/label=oSCL075a
primer_bind complement(2492..2511)
/label=oSCL075`b
primer_bind complement(2492..2511)
/label=oSCL1119
primer_bind complement(2492..2511)
/label=oSCL075`c
primer_bind complement(2495..2537)
/label=oYK472_NLS_R
primer_bind 2496..2511
/label=oLZ146 GNCR1-N F
primer_bind complement(2496..2511)
/label=oYK92_L7Ae_R
primer_bind complement(2498..2511)
/label=oYK489_GNCR1_R
primer_bind complement(2500..2511)
/label=oYK75_NLS_mcp_R
primer_bind complement(2515..2537)
/label=oYK57-MCP-mCherry-R
terminator 2611..2838
/label=bGH PA
misc_feature 2884..3312
/label=f1 origin
misc_feature 3317..3660
/label=SV40 early promoter and origin
misc_feature 3506..3583
/label=SV40 ORI
CDS 3725..4516
/label=Kan/neoR
misc_feature 4690..4820
/label=SV40 early polyadenylation signal
primer_bind complement(4856..4876)
/label=M13-rev
misc_binding complement(4882..4904)
/label=LacO
promoter complement(4909..4938)
/label=lac
misc_feature complement(5203..5876)
/label=pUC origin
CDS complement(6024..6683)
/label=AmpR
promoter complement(6923..6951)
/label=Amp prom
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gctggctagg ccaccatgga catcgagaag ctgtgcaaga aggcggaaga ggaggccaag
961 gaggcccagg agaaggccga cgagctgaga caacgacacc ccgattcgca ggcggcggaa
1021 gacgcggagg atctcgcaaa tctggctgtc gccgccgtgc tgaccgcttg tctgctggcc
1081 caggagcatc ccaacgccga tatcgctaag ctgtgcatca aggccgcgag cgaagctgcg
1141 gaggccgcgt caaaagccgc cgagctggcc caacggcacc cagacagtca agcagccaga
1201 gatgctatca aactcgcctc tcaagccgcc cgcgccgtga tcctggccat catgctcgcc
1261 gcagagaatc ccaacgctga tattgcaaag ctatgtatta aggcggccag cgaagccgca
1321 gaggctgcct cgaaagctgc ggaacttgca cagcggcacc ccgactccca ggccgcccga
1381 gatgcaatta aactggccag tcaggccgct gaggcggtgg agagagccat ctggctggcc
1441 gccgagaacc ccaatgccga tatcgccaaa aaatgcatca aagcggcctc cgaagcagcc
1501 gaggaggcct ctaaagctgc cgaagaggct caaagacacc cagacagcca gaaggcacgc
1561 gacgagatca aggaggcctc tcagaaggcc gaggaggtga aggagagatg caagagcgcc
1621 agcggaagtg gcggaggagg cgacgtcgct agcatgctca agatggagtt cgcatgtgac
1681 atctgtggtc ggaagttcgc cagaaagtac catctgtcca ggcataccag aatccatacc
1741 ggcgagaaac catttgcttg tgacatttgt ggccggaaat tcgccgatag aagcacactg
1801 agacggcata caaagatcca cacacagcgg cctcagattc cacctaagcc tttcgcctgt
1861 gacatctgtg gaaggaagtt cgctcggaaa gaccatcttc ggaaccatac acggatacac
1921 actggtgaga agcctttcgc atgtgacatc tgcggtagaa agttcgcatt cccttacctg
1981 ctgaggcgcc ataccaagat ccatactcag cggccacaaa ttccaccaaa gccattcgct
2041 tgtgatatct gtggacggaa attcgcactg aagcaccatc tgttgcggca tacccggatt
2101 cacacaggcg agaagccatt cgcctgcgac atttgcggcc ggaagttcgc tgagagatcc
2161 aaactgcgga ggcacactaa gattcacaca cagcggccac agataccacc taaaccattc
2221 gcatgcgaca tctgtggcag aaagttcgcc ttgaagcaga ccttgcaggt ccatacacgc
2281 attcacacag gtgagaaacc tttcgcttgc gatatctgcg gtcgcaagtt cgccgacaga
2341 tccaccttga gacgccatac taagatccat tccaggacct gcagagacta caaagaccat
2401 gacggcgact ataaagatca cgacatcgat tacaaagacg acgacgacaa gatggctcca
2461 aagaagaaga gaaaggtggg tgccagcgga agtggcggag gaggcgacgt ctaaggatcc
2521 actagtccag tgtggtggaa ttctgcagat atccagcaca gtggcggccg ctcgagtcta
2581 gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
2641 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
2701 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
2761 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
2821 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc
2881 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
2941 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
3001 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
3061 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
3121 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
3181 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
3241 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
3301 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
3361 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
3421 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
3481 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
3541 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc
3601 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
3661 cccgggagct tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg
3721 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
3781 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
3841 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
3901 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
3961 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
4021 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
4081 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
4141 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
4201 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga
4261 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
4321 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
4381 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
4441 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
4501 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac
4561 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
4621 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
4681 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
4741 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
4801 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg
4861 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
4921 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
4981 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
5041 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
5101 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
5161 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
5221 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
5281 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
5341 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
5401 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
5461 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
5521 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
5581 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
5641 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
5701 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
5761 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
5821 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
5881 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
5941 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
6001 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
6061 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
6121 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
6181 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
6241 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
6301 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
6361 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
6421 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
6481 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
6541 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
6601 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
6661 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
6721 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
6781 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
6841 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
6901 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
6961 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtc
//
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