pAAVLINK-EF1a-HA-5'-SPTBN1

LOCUS       pAAVLINK-EF1a-HA-5'-SPTBN1               6998 bp DNA     circular SYN 20-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6998)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 3, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6998
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    12..130
                     /label=AAV2 ITR
     promoter        158..700
                     /label=EF1-alpha
                     /note="Chimeric promoter of ef1a and htlv 5' UTR"
     promoter        187..398
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             411..679
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     misc_feature    505..510
                     /label=SDS
     CDS             716..742
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             749..4148
                     /codon_start=1
                     /label=SPTBN1
                     /translation="MTTTVATDYDNIEIQQQYSDVNNRWDVDDWDNENSSARLFERSRI
                     KALADEREAVQKKTFTKWVNSHLARVSCRITDLYTDLRDGRMLIKLLEVLSGERLPKPT
                     KGRMRIHCLENVDKALQFLKEQRVHLENMGSHDIVDGNHRLTLGLIWTIILRFQIQDIS
                     VETEDNKEKKSAKDALLLWCQMKTAGYPNVNIHNFTTSWRDGMAFNALIHKHRPDLIDF
                     DKLKKSNAHYNLQNAFNLAEQHLGLTKLLDPEDISVDHPDEKSIITYVVTYYHYFSKMK
                     ALAVEGKRIGKVLDNAIETEKMIEKYESLASDLLEWIEQTIIILNNRKFANSLVGVQQQ
                     LQAFNTYRTVEKPPKFTEKGNLEVLLFTIQSKMRANNQKVYMPREGKLISDINKAWERL
                     EKAEHERELALRNELIRQEKLEQLARRFDRKAAMRETWLSENQRLVSQDNFGFDLPAVE
                     AATKKHEAIETDIAAYEERVQAVVAVARELEAENYHDIKRITARKDNVIRLWEYLLELL
                     RARRQRLEMNLGLQKIFQEMLYIMDWMDEMKVLVLSQDYGKHLLGVEDLLQKHTLVEAD
                     IGIQAERVRGVNASAQKFATDGEGYKPCDPQVIRDRVAHMEFCYQELCQLAAERRARLE
                     ESRRLWKFFWEMAEEEGWIREKEKILSSDDYGKDLTSVMRLLSKHRAFEDEMSGRSGHF
                     EQAIKEGEDMIAEEHFGSEKIRERIIYIREQWANLEQLSAIRKKRLEEASLLHQFQADA
                     DDIDAWMLDILKIVSSSDVGHDEYSTQSLVKKHKDVAEEIANYRPTLDTLHEQASALPQ
                     EHAESPDVRGRLSGIEERYKEVAELTRLRKQALQDTLALYKMFSEADACELWIDEKEQW
                     LNNMQIPEKLEDLEVIQHRFESLEPEMNNQASRVAVVNQIARQLMHSGHPSEKEIKAQQ
                     DKLNTRWSQFRELVDRKKDALLSALSIQNYHLECNETKSWIREKTKVIESTQDLGNDLA
                     GVMALQRKLTGMERDLVAIEAKLSDLQKEAEKLESEHPDQAQAILSRLAEISDVWEEMK
                     TTLKNREASLGEASKLQQFLRDLDDFQSWLSRTQTAIASEDMPNTLTEAEKLLTQHENI
                     KNEIDNYEEDYQKMRDMGEMVTQVSI"
     misc_feature    2970..2975
                     /label=SDS
     RBS             3241..3249
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     misc_feature    3243..3248
                     /label=SDS
     misc_feature    3598..3603
                     /label=SDS
     misc_feature    3798..3803
                     /label=SDS
     misc_feature    4092..4097
                     /label=SDS
     intron          4139..4179
                     /label=Splicing Donor
     misc_feature    4183..4216
                     /label=lox2272-JT1571
     repeat_region   4261..4401
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4261..4390
                     /label=AAV2 ITR
     rep_origin      4476..4931
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5213..5317
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5318..6178
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6349..6937
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
      181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
      241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
      301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
      361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
      421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
      481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
      541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
      601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
      661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgtaccc
      721 ttatgacgta cctgactatg ctggatctat gacgaccaca gtagccacag actatgacaa
      781 cattgagatc cagcagcagt acagtgatgt caacaaccgc tgggatgtcg acgactggga
      841 caatgagaac agctctgcgc ggctttttga gcggtcccgc atcaaggctc tggcagatga
      901 gcgtgaagcc gtgcagaaga agaccttcac caagtgggtc aattcccacc ttgcccgtgt
      961 gtcctgccgg atcacagacc tgtacactga ccttcgagat ggacggatgc tcatcaagct
     1021 gctggaggtc ctctctggag agaggctgcc taaacccacc aagggacgaa tgcgcatcca
     1081 ctgcttagag aatgtggaca aggcccttca gttcctgaag gagcagagag tccatcttga
     1141 gaacatgggg tcccatgaca tcgtggatgg aaaccaccgg ctgacccttg gcctcatctg
     1201 gaccatcatc ctgcgcttcc agatccagga tatcagtgtg gaaactgaag acaacaaaga
     1261 gaagaaatct gccaaggatg cattgctgtt gtggtgccag atgaagacag ctgggtaccc
     1321 caatgtcaac attcacaatt tcaccactag ctggagggac ggcatggcct tcaatgcact
     1381 gatacacaaa caccggcctg acctgataga ttttgacaaa ctaaagaaat ctaacgcaca
     1441 ctacaacctg cagaatgcat ttaatctggc agaacagcac ctcggcctca ctaaactgtt
     1501 ggaccccgaa gacatcagcg tggaccatcc tgatgagaag tccataatca cttatgtggt
     1561 gacttattac cactacttct ctaagatgaa ggccttagct gttgaaggaa aacgaattgg
     1621 aaaggtgctt gacaatgcta ttgaaacaga aaaaatgatt gaaaagtatg aatcacttgc
     1681 ctctgacctt ctggaatgga ttgaacaaac catcatcatt ctgaacaatc gcaaatttgc
     1741 caattcactg gtcggggttc aacagcagct tcaggcattc aacacttacc gcactgtgga
     1801 gaaaccaccc aaatttactg agaaggggaa cttggaagtg ctgctcttca ccattcagag
     1861 caagatgagg gccaacaacc agaaggtcta catgccccgg gaggggaagc tcatctctga
     1921 catcaacaag gcctgggaaa gactggaaaa agcggaacac gaaagagaac tggctttgcg
     1981 gaatgagctc ataagacagg agaaactgga acagctcgcc cgcagatttg atcgcaaggc
     2041 agctatgagg gagacttggc tgagcgaaaa ccagcgtctg gtgtctcagg acaactttgg
     2101 gtttgacctt cctgcagttg aggccgccac aaaaaagcac gaggccattg agacagacat
     2161 tgccgcatac gaggagcgtg tgcaggctgt ggtagccgtg gccagggagc tcgaggccga
     2221 gaattaccac gacatcaagc gcatcacagc gaggaaggac aatgtcatcc ggctctggga
     2281 atacctactg gaactgctca gggcccggag acagcggctc gagatgaacc tggggctgca
     2341 gaagatattc caggaaatgc tctacattat ggactggatg gatgaaatga aggtgctagt
     2401 attgtctcaa gactatggca aacacttact tggtgtggaa gacctgttac agaagcacac
     2461 cctggttgaa gcagacattg gcatccaggc agagcgggtg agaggtgtca atgcctccgc
     2521 ccagaagttc gcaacagacg gggaaggtta caagccctgt gacccccagg tgatccgaga
     2581 ccgcgtggcc cacatggagt tctgttatca agagctttgc cagctggcgg ctgagcgcag
     2641 ggcccgtctg gaagagtccc gccgcctctg gaagttcttc tgggagatgg cagaagagga
     2701 aggctggata cgggagaagg agaagatcct gtcctcggac gattacggga aagacctgac
     2761 cagcgtcatg cgcctgctca gcaagcaccg ggcgttcgag gacgagatga gcggccgcag
     2821 tggccacttt gagcaggcca tcaaggaagg cgaagacatg atcgcggagg agcacttcgg
     2881 gtcggagaag atccgtgaga ggatcattta catccgggag cagtgggcca acctagagca
     2941 gctctcggcc attcggaaga agcgcctgga ggaggcctcc ctgctgcacc agttccaggc
     3001 agatgctgat gacattgatg cctggatgct ggacatcctc aagattgtct ccagcagcga
     3061 cgtgggccac gatgagtatt ccacacagtc tctggtcaag aaacacaagg acgtggcgga
     3121 agagatcgcc aattacaggc ccacccttga cacgctgcac gaacaagcca gcgccctccc
     3181 ccaggagcat gccgagtctc cagacgtgag gggcaggctg tcgggcatcg aggagcggta
     3241 taaggaggtg gcagagctga cgcggctgcg gaagcaggca ctccaggaca ctctggccct
     3301 gtacaagatg ttcagcgagg ctgatgcctg tgagctctgg atcgacgaga aggagcagtg
     3361 gctcaacaac atgcagatcc cagagaagct ggaggatctg gaggtcatcc agcacagatt
     3421 tgagagccta gaaccagaaa tgaacaacca ggcttcccgg gttgcagtgg tgaaccagat
     3481 tgcacgccag ctgatgcaca gcggccaccc aagtgagaag gaaatcaaag cccagcagga
     3541 caaactcaac acaaggtgga gccagttcag agaactggtt gacaggaaga aggatgccct
     3601 cctgtctgcc ctgagcatcc agaactacca cctcgagtgc aatgaaacca aatcctggat
     3661 tcgggaaaag accaaggtca tcgagtccac ccaggacctg ggcaatgacc tggctggcgt
     3721 catggccctg cagcgcaagc tgaccggcat ggagcgggac ttggtggcca ttgaggcaaa
     3781 gctgagtgac ctgcagaagg aggcggagaa gctggagtcc gagcaccccg accaggccca
     3841 ggccatcctg tctcggctgg ccgagatcag cgacgtgtgg gaggagatga agaccaccct
     3901 gaaaaaccga gaggcctccc tgggagaggc cagcaagctg cagcagttcc tacgggactt
     3961 ggacgacttc cagtcctggc tctctaggac ccagacagcg atcgcctcgg aggacatgcc
     4021 aaacaccctg accgaggctg agaagctgct cacgcagcac gagaacatca agaatgagat
     4081 cgacaactac gaggaggact accagaagat gagggacatg ggcgagatgg tcacccaggt
     4141 aagtatcaag gttacaagac aggtttaagg agaccaataa gctattattc gtataggata
     4201 ctttatacga agttattaag ttatggagac cggtgtggca cgtgcggacc gagcggccgc
     4261 aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg
     4321 ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc
     4381 gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt tctccttacg catctgtgcg
     4441 gtatttcaca ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg gcgcattaag
     4501 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccttagcgcc
     4561 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     4621 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     4681 aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     4741 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     4801 actcaactct atctcgggct attcttttga tttataaggg attttgccga tttcggtcta
     4861 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     4921 gtttacaatt ttatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     4981 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     5041 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     5101 atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
     5161 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
     5221 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     5281 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     5341 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     5401 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     5461 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     5521 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     5581 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     5641 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     5701 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     5761 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     5821 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     5881 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     5941 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     6001 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     6061 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     6121 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     6181 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     6241 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     6301 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     6361 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     6421 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     6481 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     6541 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     6601 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     6661 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     6721 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     6781 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     6841 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     6901 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     6961 acggttcctg gccttttgct ggccttttgc tcacatgt
//