| 质粒名称: | px330-U6-ISAam1 gRNA(Bbs1)-CAG-ISAam1-P2A-mCherry |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002339 |
| 产品用途: | A human codon-optimized ISAam1 TnpB and chimeric guide RNA expression plasmid. |
| 存储实验室: | Guanghai Xiang lab |
|
载体骨架基本信息 |
|
| 载体名称: | px330 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CAG |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Dh5a |
|
插入基因信息 |
|
| 插入基因名称: | Nuclease, mCherry |
| 物种来源: |
Synthetic
|
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Comprehensive assessment of activity, specificity, and safety of hypercompact TnpB systems for gene editing.
Xin C., Xiang G., Cao S., Wang Y., Yuan S., Liu X., Huo Y., Sun J., Wan X., Liu D., Hong J., Hu J., Wang H.
Genome Biol. 2026 (2026)
|
px330-U6-ISAam1 gRNA(Bbs1)-CAG-ISAam1-P2A-mCherry
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
px330-U6-ISAam1 gRNA(Bbs1)-CAG-ISAam1-P2A-mCherry载体质粒基本信息
px330-U6-ISAam1 gRNA(Bbs1)-CAG-ISAam1-P2A-mCherry质粒图谱载体图谱和px330-U6-ISAam1 gRNA(Bbs1)-CAG-ISAam1-P2A-mCherry载体序列质粒序列多克隆位点信息
px330-U6-ISAam1 gRNA(Bbs1)-CAG-ISAam1-P2A-mCherry质粒载体简介
px330-U6-ISAam1 gRNA(Bbs1)-CAG-ISAam1-P2A-mCherry质粒序列载体序列
LOCUS px330-U6-ISAam1 gRNA(Bbs1)-CAG-ISAam1-P2A-mCherry 6247 bp DNA circular SYN 20-APR-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6247)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Aug 26, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6247
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin 1..589
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
promoter 651..899
/label=U6 promoter
/note="RNA polymerase III promoter for human U6 snRNA
(Domitrovich & Kunkel, 2003)"
enhancer 1119..1404
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer;
contains an 18-bp deletion relative to the standard CMV
enhancer"
promoter 1406..1682
/label=chicken beta-actin promoter
/note="contains a 21-bp deletion relative to the standard
chicken beta-actin promoter"
promoter 1527..1546
/label=chicken beta-actin promoter
/note="contains a 21-bp deletion relative to the standard
chicken beta-actin promoter"
intron 1683..1910
/label=hybrid intron
/note="hybrid between chicken beta-actin (CBA) and minute
virus of mice (MMV) introns (Gray et al., 2011)"
regulatory 1922..1931
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 1928..3088
/codon_start=1
/label=ISAam1_humanized
/translation="MAPKKKRKVGIHGVPAATSVNKSYKFRLYPTKEQEQLLAKTFGCV
RFVYNKMLEERIQIYEKFKDDKEALKKQTFPTPAKYKKEFPWLKEVDSLALANAQLNLQ
KAFQNFFSGRAGFPKFKNRKAKQSYTTNVVNGNIQLSDGYIKLPKLKWVKFKQHREIPA
HHIIKACTIKKTKTGKYYVSILTEYEHQPVPKEIQTVVGLDFSMNGLFVDSEGKRANYP
RFYRQALEKLAKEQRILSRRKKGSNRWHKQRLKVAKLHEKIANQRKDFLHKKSYELAKQ
YDCVVIENLNMKGMSQVLNFGKSVHDNGWGMFTTFLQYKLEEQGKKLIKIDKWFPSSKT
CSCCDQVKESLSLGERTFRCECGFESDRDVNAAINIKHEGMKRLAIA"
CDS 1934..1954
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
misc_feature 2127..2132
/label=SDS
misc_feature 2193..2198
/label=SDS
CDS 3089..3136
/codon_start=1
/product="bipartite nuclear localization signal from
nucleoplasmin"
/label=nucleoplasmin NLS
/translation="KRPAATKKAGQAKKKK"
CDS 3146..3202
/codon_start=1
/product="2A peptide from porcine teschovirus-1
polyprotein"
/label=P2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="ATNFSLLKQAGDVEENPGP"
CDS 3203..3913
/codon_start=1
/product="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
/label=mCherry
/note="mammalian codon-optimized"
/translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
DDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
EGRHSTGGMDELYK"
misc_feature 3219..3224
/label=SDS
misc_feature 3651..3656
/label=SDS
polyA_signal 3944..4151
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
repeat_region 4160..4300
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4160..4289
/label=AAV2 ITR
rep_origin 4375..4830
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5112..5216
/gene="bla"
/label=AmpR promoter
CDS 5217..6077
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
ORIGIN
1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt gagggcctat
661 ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag ataattggaa
721 ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga aagtaataat
781 ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat atgcttaccg
841 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgaaacaccg
901 acagggacgt caatgcggca atcaatatca aacatgaggg catgaaacga ttagcaatag
961 cctaacttgt cctcgaaccg tgggacacac ggggatcgct cagtcaactt cccgtcatga
1021 gatgggatta cctgagaagc ccccacctct aagcgaagcg taggtggtgg gagcatgtca
1081 ctcggatatg gtaagacatg cttttttcta gaggtacccg ttacataact tacggtaaat
1141 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaatagt aacgccaata
1201 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
1261 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
1321 gcctggcatt gtgcccagta catgacctta tgggactttc ctacttggca gtacatctac
1381 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
1441 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
1501 gcgatggggg cggggggggg ggggggcgcg cgccaggcgg ggcggggcgg ggcgaggggc
1561 ggggcggggc gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt
1621 ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg
1681 cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc cgccgccgcc tcgcgccgcc
1741 cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc
1801 ctccgggctg taattagctg agcaagaggt aagggtttaa gggatggttg gttggtgggg
1861 tattaatgtt taattacctg gagcacctgc ctgaaatcac tttttttcag gttggaccgg
1921 tgccaccatg gccccaaaga agaagcggaa ggtcggtatc cacggagtcc cagcagccac
1981 tagtgtgaac aagagctaca agttcaggct gtaccccacc aaggagcagg agcagctgct
2041 ggctaagacc ttcggctgcg tgaggttcgt gtacaacaag atgctggagg agaggattca
2101 aatctacgag aaattcaagg acgacaagga ggccctgaag aagcagacct tccccacccc
2161 tgccaaatac aagaaggagt tcccctggct gaaggaggtg gacagcctgg ctctggctaa
2221 cgctcagctg aacctgcaga aggcctttca gaacttcttc tccggccgcg ccggctttcc
2281 taagttcaag aacagaaagg ccaagcagag ctacaccaca aacgtggtga acgggaacat
2341 ccagctgagc gacggataca tcaagctgcc caagctgaaa tgggtgaagt tcaagcagca
2401 cagagagatc ccagcccacc acatcatcaa ggcctgcacc atcaagaaaa ccaagaccgg
2461 caaatactac gtgagcatcc tgaccgaata cgaacaccag cccgtgccca aggaaatcca
2521 gaccgtggtg ggactggact tcagcatgaa cgggctgttc gtggacagcg aaggcaagag
2581 ggccaactat cccagattct acagacaggc cctggagaaa ctggccaagg agcagagaat
2641 cctgagtaga cggaaaaagg gaagcaacag atggcataag cagagactga aagtggccaa
2701 gctgcacgaa aagatcgcca accagagaaa ggacttcctg cacaagaaga gctacgagct
2761 ggccaaacag tacgactgcg tggtgatcga gaacctgaac atgaaaggaa tgagtcaggt
2821 gctgaacttc ggcaagagcg tgcacgacaa cggctgggga atgttcacaa cctttctgca
2881 gtacaaactg gaggagcagg gaaaaaagct gatcaagatc gacaagtggt tccccagcag
2941 caagacctgc agctgctgcg accaggtgaa ggagagcctg agcctgggcg agagaacctt
3001 cagatgcgag tgcggcttcg agagcgacag agacgtgaac gccgccatca acatcaagca
3061 cgagggcatg aagagactgg ccatcgccaa aaggccggcg gccacgaaaa aggccggcca
3121 ggcaaaaaag aaaaagggaa gcggagctac taacttcagc ctgctgaagc aggctggaga
3181 cgtggaggag aaccctggac ctatggtgag caagggcgag gaggataaca tggccatcat
3241 caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga
3301 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt
3361 gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg
3421 ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc
3481 cgagggcttc aagtgggagc gcgtgatgaa cttcgatgac ggcggcgtgg tgaccgtgac
3541 ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa
3601 cttcccctcc gacggccccg taatgcagaa gaaaaccatg ggctgggagg cctcctccga
3661 gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa
3721 ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca
3781 gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta
3841 caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga
3901 gctgtacaag taagaattcc tagagctcgc tgatcagcct cgactgtgcc ttctagttgc
3961 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
4021 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
4081 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga gaatagcagg
4141 catgctgggg agcggccgca ggaaccccta gtgatggagt tggccactcc ctctctgcgc
4201 gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg
4261 gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt
4321 ctccttacgc atctgtgcgg tatttcacac cgcatacgtc aaagcaacca tagtacgcgc
4381 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
4441 ttgccagcgc cttagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
4501 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
4561 tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc
4621 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
4681 tgttccaaac tggaacaaca ctcaactcta tctcgggcta ttcttttgat ttataaggga
4741 ttttgccgat ttcggtctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
4801 attttaacaa aatattaacg tttacaattt tatggtgcac tctcagtaca atctgctctg
4861 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
4921 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
4981 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc
5041 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
5101 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
5161 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
5221 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
5281 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
5341 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
5401 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
5461 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
5521 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
5581 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
5641 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
5701 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
5761 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
5821 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
5881 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt ggaagccgcg
5941 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
6001 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
6061 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
6121 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
6181 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
6241 gatcttc
//
