| 质粒名称: | pAAV-CAG-NES-GAF-CaMP3-sfGFP |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000161 |
| 产品用途: | Expresses the GAF-CaMP3-sfGFP far-red/green bacteriophytochrome-based positive calcium indicator in the cytosol of mammalian cells |
| 存储实验室: | Laboratory of Molecular Design |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV |
| 表达系统: | 腺病毒，哺乳动物表达 |
| 启动子: | CAG |
| 拷贝数: | 低拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Mach1 |
|
插入基因信息 |
|
| 插入基因名称: | GAF-CaMP3 |
| 物种来源: | Synthetic |
| 密码子优化: | 细菌表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
GAF-CaMP3-sfGFP, An Enhanced Version of the Near-Infrared Genetically Encoded Positive Phytochrome-Based Calcium Indicator for the Visualization of Neuronal Activity
Subach O.M., Subach F.V.
Int. J. Mol. Sci. 2020, Vol. 21, Page 6883 21, 6883 (2020)
|
买家导航
pAAV-CAG-NES-GAF-CaMP3-sfGFP载体质粒基本信息
pAAV-CAG-NES-GAF-CaMP3-sfGFP质粒图谱载体图谱和pAAV-CAG-NES-GAF-CaMP3-sfGFP载体序列质粒序列多克隆位点信息
pAAV-CAG-NES-GAF-CaMP3-sfGFP质粒载体简介
pAAV-CAG-NES-GAF-CaMP3-sfGFP质粒序列载体序列
LOCUS pAAV-CAG-NES-GAF-CaMP3-sfGFP 6583 bp DNA circular SYN 22-APR-2023
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6583)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 28, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6583
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin complement(182..770)
/direction=LEFT
/label=ori
/note="high-copy-number colE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(941..1801)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(1802..1906)
/gene="bla"
/label=AmpR promoter
rep_origin complement(1932..2387)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2942..3219
/label=chicken beta-actin promoter
CDS 3652..3678
/codon_start=1
/product="nuclear export signal from the HIV Rev protein
(Fischer et al., 1995)"
/label=NES
/translation="LPPLERLTL"
CDS 3688..4740
/codon_start=1
/label=GAF-CaMP3
/translation="MRAGPSIDQSGMLTPALERIRAAESLRALCDDTALLFRRCTGYDR
VMVYRFGAHGQDQVFSECRAPGLESYLGNRYPSSMVPRLARQLYLRQRVRMRADVAYLG
RPTSPLPDQLTEEQIAEFKEAFTLFDKDGDGTVTTKELGAVMRSLGQNPTEAELRVMID
EVDADGDGTLDCPEFLAMMARRMKYRDTEEEIREAFGVVDADGNGYISAAELRHVLTSL
GEKLTDEEVGELIREAAINGDGQVNCEEFVQMMTARGDSGGGCSSRRMWNKVGHAVRAI
GRLSSGGCHLRYLNDMGVRASLVVSLVVGGKLWGLVVCYHYLPRYIHHELRAYCRSLAD
GIAARIAALES"
primer_bind 3911..3932
/label=WKG160-F1
primer_bind 4510..4532
/label=WKG160-F2
CDS 4747..5466
/codon_start=1
/product="enhanced CFP"
/label=sfGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLL
EFVTAAGITHGMDELYK"
misc_feature 5485..6073
/label=WPRE
/note="woodchuck posttranscriptional regulatory element"
CDS complement(5956..5967)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
polyA_signal 6116..6237
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
ORIGIN
1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
841 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
901 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
961 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
1021 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
1081 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
1141 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
1201 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
1261 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
1321 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
1381 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
1441 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
1501 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
1561 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
1621 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
1681 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
1741 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
1801 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
1861 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
1921 aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
1981 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
2041 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
2101 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
2161 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
2221 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
2281 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
2341 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt
2401 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
2461 gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc gggcgtcggg cgacctttgg
2521 tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga gtggccaact ccatcactag
2581 gggttccttg tagttaatga ttaacccgcc atgctactta tctacgtagc catgctctag
2641 gaagatcgta ggttaattaa ggcgcgcccc taggggagat ggccagccag cccattgacg
2701 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
2761 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
2821 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
2881 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
2941 gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca
3001 attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg gggggggggg
3061 gggcgcgcgc caggcggggc ggggcggggc gaggggcggg gcggggcgag gcggagaggt
3121 gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg
3181 cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc gacgctgcct
3241 tcgccccgtg ccccgctccg ccgccgcctc gcgccgcccg ccccggctct gactgaccgc
3301 gttactccca caggtgagcg ggcgggacgg cccttctcct ccgggctgta attagcgctt
3361 ggtttaatga cggcttgttt cttttctgtg gctgcgtgaa agccttgagg ggctccggga
3421 gggccctttg tgcgggggga gcggctcggg gctgtccgcg gggggacggc tgccttcggg
3481 ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agagcctctg
3541 ctaaccatgt tcatgccttc ttctttttcc tacagctcct gggcaacgtg ctggttattg
3601 tgctgtctca tcattttggc aaagaattgg ggatccgaca ccatgcttca acttcctcct
3661 cttgaacgtc ttactctttc gagatctatg cgggcaggtc catccattga tcagtctgga
3721 atgttgacgc ccgcccttga gaggattagg gctgccgaga gccttcgggc cctctgtgac
3781 gacaccgctt tgctttttcg acgatgtacc ggctacgacc gtgtaatggt gtacagattt
3841 ggcgctcatg gacaggacca ggtatttagt gagtgtcgtg cgccgggact ggagagctac
3901 ctgggtaacc gctaccctag ctcaatggtt ccacgattgg caaggcaact gtatctgcgg
3961 caacgagttc gaatgagggc ggacgtggct tacctagggc ggccaacgag ccctttgccg
4021 gaccaactga ccgaagagca gatcgcagaa tttaaagagg ctttcaccct atttgacaag
4081 gacggggatg ggacagtaac caccaaggag ctgggggcgg tgatgcggtc tctggggcag
4141 aaccccacag aagcagagct gcgggtcatg atcgatgaag tagatgccga cggtgacggc
4201 actctcgact gccctgagtt cctggcaatg atggcaagaa gaatgaaata cagggacacg
4261 gaagaagaaa ttagagaagc gttcggtgtg gttgatgcgg atggcaatgg ctacatcagt
4321 gcagcagagc ttcgccatgt gttgacaagc cttggagaga agttaacaga tgaagaggtt
4381 ggtgaattga tcagggaagc agccatcaat ggggatggcc aggtgaactg cgaggagttc
4441 gtacaaatga tgacagcgag gggcgattca ggaggcgggt gctcatcgcg tcgtatgtgg
4501 aataaggtag gtcacgcagt cagagctata ggtcggctga gctccggtgg ttgtcatctc
4561 cggtacctca atgatatggg ggtccgcgcc tcattggttg taagcctggt tgtaggcgga
4621 aaactttggg ggctggtggt gtgttaccat tatcttccga gatacatcca ccatgaactc
4681 agggcatatt gcagaagtct tgcggatgga attgcggcga gaatagctgc ccttgagtct
4741 gaattcatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
4801 ctggacggcg acgtaaacgg ccacaagttc agcgtgcgcg gcgagggcga gggcgatgcc
4861 accaacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
4921 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac
4981 atgaagcgcc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
5041 atcagcttca aggacgacgg cacctacaag acccgcgccg aggtgaagtt cgagggcgac
5101 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
5161 gggcacaagc tggagtacaa cttcaacagc cacaacgtct atatcaccgc cgacaagcag
5221 aagaacggca tcaaggccaa cttcaagatc cgccacaacg tggaggacgg cagcgtgcag
5281 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
5341 aaccactacc tgagcaccca gtccgtgctg agcaaagacc ccaacgagaa gcgcgatcac
5401 atggtcctgc tggagttcgt gaccgccgcc gggatcactc acggcatgga cgagctgtat
5461 aagtaagaat tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg
5521 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
5581 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
5641 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
5701 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
5761 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
5821 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
5881 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
5941 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
6001 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
6061 gccgcctccc cgcatcgata ccgtcgaccc gggcggccgc ttcgagcaga catgataaga
6121 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
6181 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
6241 aacaacaatt gcattcattt tatgtttcag gttcaggggg agatgtggga ggttttttaa
6301 agcaagtaaa acctctacaa atgtggtaaa atcgataagg atcttcctag agcatggcta
6361 cgtagataag tagcatggcg ggttaatcat taactacaag gaacccctag tgatggagtt
6421 ggccactccc tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg
6481 acgcccgggc tttgcccggg cggcctcagt gagcgagcga gcgcgcagct gcattaatga
6541 atcggccaac gcgcggggag aggcggtttg cgtattgggc gct
//
pAAV-CAG-NES-GAF-CaMP3-sfGFP载体图谱质粒图谱pdf版和pAAV-CAG-NES-GAF-CaMP3-sfGFP载体序列质粒序列等相关资料下载
pAAV-CAG-NES-GAF-CaMP3-sfGFP质粒载体应用举例
- 上一篇：pAAV-CAG-NES-GAF-CaMP2-sfGFP
- 下一篇：没有了
