pDisplay-HA1m-ires-mCherry

价格：元

规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg

相关技术服务：质粒构建    基因合成    质粒大提

联系方式：I47-825O-882O

买家导航

pDisplay-HA1m-ires-mCherry载体质粒基本信息

质粒名称: pDisplay-HA1m-ires-mCherry
出品公司: Wekwikgene
目录编号: 0000841
产品用途: Express the medium-affinity histamine sensor GRAB_HA1m in mammalian cells
存储实验室: KIRYL PIATKEVICH Lab

载体骨架基本信息
载体名称: pDisplay
表达系统: 哺乳动物表达
启动子: CMV
拷贝数: 高拷贝
载体抗性: 氨苄霉素
生长温度: 37 ℃
宿主菌: TOP10

插入基因信息
插入基因名称: Histamine 4R
物种来源: Human
密码子优化: 未优化
 

pDisplay-HA1m-ires-mCherry质粒图谱载体图谱和pDisplay-HA1m-ires-mCherry载体序列质粒序列多克隆位点信息

pDisplay-HA1m-ires-mCherry载体图谱

pDisplay-HA1m-ires-mCherry质粒载体简介

pDisplay-HA1m-ires-mCherry质粒序列载体序列

LOCUS       pDisplay-HA1m-ires-mCherry             8584 bp DNA     circular DNA 23-DEC-2024
DEFINITION  pDisplay-BirA-ER.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8584)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 29, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..8584
                     /mol_type="other DNA"
                     /organism="pDisplay-BirA-ER"
     promoter        11..627
                     /label=CMV_immearly_promoter
                     /label=CMV_immearly_promoter
                     /label=CMV_immearly_promoter
                     /label=CMV_immearly_promoter
                     /label=CMV_immearly_promoter
     misc_feature    90..377
                     /label=CAG_enhancer
                     /label=CAG_enhancer
                     /label=CAG_enhancer
                     /label=CAG_enhancer
                     /label=CAG_enhancer
     misc_feature    544..564
                     /label=CMV_fwd_primer
                     /label=CMV_fwd_primer
                     /label=CMV_fwd_primer
                     /label=CMV_fwd_primer
                     /label=CMV_fwd_primer
     primer_bind     549..566
                     /label=PCMV5F
     promoter        638..656
                     /label=T7_promoter
                     /label=T7_promoter
                     /label=T7_promoter
                     /label=T7_promoter
                     /label=T7_promoter
     CDS             721..2775
                     /label=WBH1-NE
     misc_feature    721..783
                     /label=Ig K leader sequence
                     /label=Ig K leader sequence
     primer_bind     complement(795..810)
                     /label=pDEST-vector-rev
     gene            817..2775
                     /label=WBH1
     variation       1120..1122
                     /label=2.43
                     /label=nonstandard type:  mutation
     variation       1243..1245
                     /label=3.32
                     /label=nonstandard type:  mutation
     variation       1249..1251
                     /label=3.34
                     /label=nonstandard type:  mutation
     variation       1255..1257
                     /label=3.36
                     /label=nonstandard type:  mutation
     primer_bind     1490..1509
                     /label=9-HRH4-F
     variation       1507..1509
                     /label=5.42
                     /label=nonstandard type:  mutation
     variation       1510..1512
                     /label=5.43
                     /label=nonstandard type:  mutation
     variation       1573..1575
                     /label=5.64
                     /label=nonstandard type:  mutation
     variation       1588..1590
                     /label=5.69
                     /label=nonstandard type:  mutation
     variation       1591..1593
                     /label=5.70
                     /label=nonstandard type:  mutation
     variation       1597..1599
                     /label=5.72
                     /label=nonstandard type:  mutation
     gene            1600..2403
                     /label=NE ICL3
     Region          1654..2376
                     /label=cpEGFP
     variation       2404..2406
                     /label=6.31
                     /label=nonstandard type:  mutation
     variation       2407..2409
                     /label=6.32
                     /label=nonstandard type:  mutation
     variation       2410..2412
                     /label=6.33
                     /label=nonstandard type:  mutation
     variation       2413..2415
                     /label=6.34
                     /label=nonstandard type:  mutation
     variation       2416..2418
                     /label=6.35
                     /label=nonstandard type:  mutation
     variation       2464..2466
                     /label=6.51
                     /label=nonstandard type:  mutation
     variation       2467..2469
                     /label=6.52
                     /label=nonstandard type:  mutation
     variation       2533..2535
                     /label=7.39
                     /label=nonstandard type:  mutation
     variation       2542..2544
                     /label=7.42
                     /label=nonstandard type:  mutation
     primer_bind     complement(2756..2809)
                     /label=R-IRES-NheI-HRH4
     primer_bind     2776..2794
                     /label=HTR2C-IRES-fwd
     gene            2782..3365
                     /label=IRES
     gene            3372..4076
                     /label=mCherry
     gene            4077..4127
                     /label=CAAX
     primer_bind     complement(4102..4158)
                     /label=pDest-CAAX-rev
     primer_bind     4128..4152
                     /label=pDEST-vector-fwd
     misc_feature    4164..4173
                     /label=PDGFR transmembrane domain
                     /label=PDGFR transmembrane domain
     misc_feature    complement(4335..4352)
                     /label=BGH_rev_primer
                     /label=BGH_rev_primer
                     /label=BGH_rev_primer
                     /label=BGH_rev_primer
                     /label=BGH_rev_primer
                     /note="/translation=METDTLLLWVLLLWVPGSTGDYPYDVPDYAGAQPARSMK
                     DNTVP 
                     LKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDWGVDVFTVPGKGYSLPEPIQ 
                     LLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGDACIAEYQQAGRGRRGRKW 
                     FSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKLGADKVRVKWPNDLYLQD 
                     RKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGWITLQEAGINLDRNTLA 
                     AMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIFGISRGIDKQGA 
                     LLLEQDGIIKPWMGGEISLRSAEKGSTSGSGGSKDEL*"
     terminator      4338..4565
                     /label=bGH_PA_terminator
                     /label=bGH_PA_terminator
                     /label=bGH_PA_terminator
                     /label=bGH_PA_terminator
                     /label=bGH_PA_terminator
                     /note="/translation=METDTLLLWVLLLWVPGSTGDYPYDVPDYAGAQPARSMK
                     DNTVP 
                     LKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDWGVDVFTVPGKGYSLPEPIQ 
                     LLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGDACIAEYQQAGRGRRGRKW 
                     FSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKLGADKVRVKWPNDLYLQD 
                     RKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGWITLQEAGINLDRNTLA 
                     AMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIFGISRGIDKQGA 
                     LLLEQDGIIKPWMGGEISLRSAEKGSTSGSGGSKDEL*"
     rep_origin      complement(4682..5301)
                     /direction=LEFT
                     /label=pBR322_origin
                     /label=pBR322_origin
                     /label=pBR322_origin
                     /label=pBR322_origin
                     /label=pBR322_origin
                     /note="/translation=METDTLLLWVLLLWVPGSTGDYPYDVPDYAGAQPARSMK
                     DNTVP 
                     LKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDWGVDVFTVPGKGYSLPEPIQ 
                     LLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGDACIAEYQQAGRGRRGRKW 
                     FSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKLGADKVRVKWPNDLYLQD 
                     RKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGWITLQEAGINLDRNTLA 
                     AMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIFGISRGIDKQGA 
                     LLLEQDGIIKPWMGGEISLRSAEKGSTSGSGGSKDEL*"
     primer_bind     4756..4773
                     /label=f-xa.7
     primer_bind     complement(4758..4775)
                     /label=f-xa.J
     primer_bind     complement(5224..5241)
                     /label=f-xa.G
     terminator      complement(5449..5718)
                     /label=TK_PA_terminator
                     /label=TK_PA_terminator
                     /label=TK_PA_terminator
                     /label=TK_PA_terminator
                     /label=TK_PA_terminator
                     /note="/translation=METDTLLLWVLLLWVPGSTGDYPYDVPDYAGAQPARSMK
                     DNTVP 
                     LKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDWGVDVFTVPGKGYSLPEPIQ 
                     LLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGDACIAEYQQAGRGRRGRKW 
                     FSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKLGADKVRVKWPNDLYLQD 
                     RKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGWITLQEAGINLDRNTLA 
                     AMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIFGISRGIDKQGA 
                     LLLEQDGIIKPWMGGEISLRSAEKGSTSGSGGSKDEL*"
     CDS             5842..6378
                     /codon_start=1
                     /label=ORF frame 2
                     /label=ORF frame 2
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEAV
                     SPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAIGHDEILAVG
                     HARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQPPH
                     CISHDGYFLGRSKVR"
     CDS             complement(5893..6687)
                     /codon_start=1
                     /label=ORF frame 3
                     /label=ORF frame 3
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTYGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     misc_feature    complement(6786..6805)
                     /label=SV40pro_F_primer
                     /label=SV40pro_F_primer
                     /label=SV40pro_F_primer
                     /label=SV40pro_F_primer
                     /label=SV40pro_F_primer
                     /note="/translation=MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRL
                     SAQGR 
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL 
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDQEH 
                     QGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQD 
                     IALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     rep_origin      complement(6790..6867)
                     /direction=LEFT
                     /label=SV40_origin
                     /label=SV40_origin
                     /label=SV40_origin
                     /label=SV40_origin
                     /label=SV40_origin
                     /note="/translation=MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRL
                     SAQGR 
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL 
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDQEH 
                     QGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQD 
                     IALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     misc_feature    7032..7052
                     /label=pBABE_3_primer
                     /label=pBABE_3_primer
                     /label=pBABE_3_primer
                     /label=pBABE_3_primer
                     /label=pBABE_3_primer
                     /note="/translation=MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRL
                     SAQGR 
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL 
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDQEH 
                     QGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQD 
                     IALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     gene            complement(7135..7995)
                     /gene="Ampicillin"
                     /label=Ampicillin
                     /label=Ampicillin
                     /label=Ampicillin
                     /label=Ampicillin
                     /label=Ampicillin
                     /note="/translation=MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRL
                     SAQGR 
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL 
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDQEH 
                     QGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQD 
                     IALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     CDS             complement(7135..7995)
                     /codon_start=1
                     /label=ORF frame 2
                     /label=ORF frame 2
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7346..7363
                     /label=f-xa.11
     primer_bind     complement(7352..7369)
                     /label=fxaE
     primer_bind     complement(7795..7812)
                     /label=f-xa.C
     primer_bind     7971..7988
                     /label=pRLTK.R
     promoter        complement(8037..8065)
                     /label=AmpR_promoter
                     /label=AmpR_promoter
                     /label=AmpR_promoter
                     /label=AmpR_promoter
                     /label=AmpR_promoter
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     EIGASLIKHW*"
     rep_origin      8144..8450
                     /label=f1_origin
                     /label=f1_origin
                     /label=f1_origin
                     /label=f1_origin
                     /label=f1_origin
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     EIGASLIKHW*"
     primer_bind     8226..8243
                     /label=f-xa.15
ORIGIN
        1 gcgcgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
       61 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      121 gaccgcccaa cgacccctgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      181 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
      241 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      301 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      361 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
      421 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
      481 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
      541 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc
      601 taactagaga acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga
      661 cccaagcttg gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattcgg
      721 atggagacag acacactcct gctatgggta ctgctgctct gggttccagg ttccactggt
      781 gacacaagtt tgtacaaaaa agttggcacc accggtatgg agcccgccgg cgccggcggc
      841 gccgccattc tgctggacga cgacgacgac tactacctca caacccccgg ctaccccggc
      901 tccggctcta acgcctccct gtctcctcca gccgacctgt tccccgccgc ctctaaccac
      961 tcgaccgctg tcgtggtgtc tggcgacctg gacctgtacg tgatcattcc actgggcatc
     1021 ttcctcgccg tgctgtctct cgtgacattc gccggcaacg tgatggtgct gcacgccctc
     1081 agaacagaga agaagctcca aacagtgtct aacattttca tcatgtctct cgccattgcc
     1141 gactgctctg tgggcctgtt cgccatgcca ctgagcgccg tgtacgccct gctgggctac
     1201 tggcctttcg gcctcgtggt gtgcaacatg tggttgagta tggactacgt ggccagcaca
     1261 gcctctattt tcaacctgtt cgccctgtcc ctcgaccggt tctggtctat tatttctcca
     1321 ctcagatacc tcaagaagcg gacacgcaag cgggccatta ttatgtgctc tagcgtgtgg
     1381 ctcgcctctc tcctgtggat tatcccggtt gtaggatggc accagatgtt caacgacggc
     1441 aggcgggacg tgccagtgga ggagtgcgac accgagttcg ccgacgagca ggtgttcaag
     1501 gtgctgacag ccatcttcaa cttctacgtg cctatggccg ccatgattgc cctgtacggc
     1561 aggattttcc acgagattaa gctcagagcc gcgtctcagc cagcaccggc cgggccgcgc
     1621 gacaccgacg cgctggacct ggaggaggga ggaaacgtct atatcaaggc cgacaagcag
     1681 aagaacggca tcaaggcgaa cttcgtgatc cgccacaaca tcgaggacgg cggcgtgcag
     1741 ctcgcctacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     1801 aaccactacc tgagcgtgca gtccaaactt tcgaaagacc ccaacgagaa gcgcgatcac
     1861 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     1921 aagggcggta ccggagggag catggtgaga aagggcgagg agctgttcac cggggtggtg
     1981 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     2041 ggtgagggcg atgccaccga gggcaagctg accctgaagt tcatctgcac caccggcaag
     2101 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
     2161 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     2221 atccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     2281 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     2341 gacggcaaca tcctggggca caagctggag tacaacaccg gagcagcagc acgctggcgc
     2401 gcgaagaagg ccgccttcca gctgggcgtg atcctgggcg ccttctgcct gtgcttcatg
     2461 ccttacttca ttagcttcat cattaccgcc ttctgcaagg actgcgtgtc tcctgccgtg
     2521 cactctattt tcacatggct gggctacgtg aactctacaa caaacccttt cctgtacaca
     2581 tggacaaacg ccaacttccg gtccgccttc cggaagatcc tccggatgaa ggagacacca
     2641 aagaagccag tgtacatgca gatgcctcac aagaccccta gaccaaagaa gcagccatta
     2701 gaagctaggg tgagcggcac agcctctggc ggcaccacag ccccagcccg caagtctgac
     2761 gacaccagac tctaagctag cgcccctctc cctccccccc ccctaacgtt actggccgaa
     2821 gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt
     2881 cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg
     2941 gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc
     3001 ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc
     3061 ccccacctgg cgacaggtgc ctctgcggcc aacgccacgt gtataagata cacctgcaaa
     3121 ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct
     3181 ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg
     3241 atctgatctg gggcctcggt acacatgctt tacatgtgtt tagtcgaggt taaaaaaacg
     3301 tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataatatgg
     3361 ccacaaccat ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc
     3421 gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg
     3481 gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc
     3541 ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg
     3601 tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt
     3661 gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc
     3721 tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg
     3781 gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg
     3841 aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact
     3901 acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct
     3961 acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac
     4021 agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaaggatg
     4081 gtaaaaagaa gaaaaagaag tcaaagacaa agtgtgtaat tatgtaagtc gacgaacaaa
     4141 aactcatctc agaagaggat ctgaatgctg tgggccagga cacgcaggag gtcatcgtgg
     4201 tgccacactc cttgcccttt aaggtggtgg tgatctcagc catcctggcc ctggtggtgc
     4261 tcaccatcat ctcccttatc atcctcatca tgctttggca gaagaagcca cgttaggcgg
     4321 ccgctcgaga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     4381 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     4441 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     4501 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
     4561 tatggcttct gaggcggaaa gaaccagtgg cggtaatacg gttatccaca gaatcggggg
     4621 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     4681 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     4741 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     4801 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     4861 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     4921 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     4981 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacgcgac ttatcgccac
     5041 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     5101 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     5161 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     5221 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     5281 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     5341 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     5401 aaaaatgaag ttttaaatca atctaaagta tatatgagta acctgaggct atggcagggc
     5461 ctgccgcccc gacgttggct gcgagccctg ggccttcacc cgaacttggg gggtggggtg
     5521 gggaaaagga agaaacgcgg gcgtattggc cccaatgggg tctcggtggg gtatcgacag
     5581 agtgccagcc ctgggaccga accccgcgtt tatgaacaaa cgacccaaca ccgtgcgttt
     5641 tattctgtct ttttattgcc gtcatagcgc gggttccttc cggtattgtc tccttccgtg
     5701 tttcagttag cctcccccta gggtgggcga agaactccag catgagatcc ccgcgctgga
     5761 ggatcatcca gccggcgtcc cggaaaacga ttccgaagcc caacctttca tagaaggcgg
     5821 cggtggaatc gaaatctcgt gatggcaggt tgggcgtcgc ttggtcggtc atttcgaacc
     5881 ccagagtccc gctcagaaga actcgtcaag aaggcgatag aaggcgatgc gctgcgaatc
     5941 gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc cattcgccgc caagctcttc
     6001 agcaatatca cgggtagcca acgctatgtc ctgatagcgg tccgccacac ccagccggcc
     6061 acagtcgatg aatccagaaa agcggccatt ttccaccatg atattcggca agcaggcatc
     6121 gccataggtc acgacgagat cctcgccgtc gggcatgctc gccttgagcc tggcgaacag
     6181 ttcggctggc gcgagcccct gatgctcttc gtccagatca tcctgatcga caagaccggc
     6241 ttccatccga gtacgtgctc gctcgatgcg atgtttcgct tggtggtcga atgggcaggt
     6301 agccggatca agcgtatgca gccgccgcat tgcatcagcc atgatggata ctttctcggc
     6361 aggagcaagg tgagatgaca ggagatcctg ccccggcact tcgcccaata gcagccagtc
     6421 ccttcccgct tcagtgacaa cgtcgagcac agctgcgcaa ggaacgcccg tcgtggccag
     6481 ccacgatagc cgcgctgcct cgtcttgcag ttcattcagg gcaccggaca ggtcggtctt
     6541 gacaaaaaga accgggcgcc cctgcgctga cagccggaac acggcggcat cagagcagcc
     6601 gattgtctgt tgtgcccagt catagccgaa tagcctctcc acccaagcgg ccggagaacc
     6661 tgcgtgcaat ccatcttgtt caatcatgcg aaacgatcct catcctgtct cttgatcgat
     6721 ctttgcaaaa gcctaggcct ccaaaaaagc ctcctcacta cttctggaat agctcagagg
     6781 ccgggcctcg gcctctgcat aaataaaaaa aattagtcag ccatggggcg gagaatgggc
     6841 ggaactgggc ggagttaggg gcggaatggg cggagttagg ggcgggacta tggttgctga
     6901 ctaattgaga tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca
     6961 cctggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg gggagcctgg
     7021 ggactttcca caccctaact gacacacatt ccacagctgg ttctttccgc ctcaggactc
     7081 ttcctttttc aataaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     7141 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     7201 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     7261 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     7321 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     7381 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     7441 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     7501 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     7561 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     7621 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     7681 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     7741 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     7801 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     7861 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     7921 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     7981 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     8041 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     8101 cacatttccc cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg
     8161 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     8221 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     8281 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     8341 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     8401 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     8461 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     8521 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
     8581 ttac
//

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