| 质粒名称: | px330-U6-ISDge10 gRNA(PciI XbaI)-CAG-ISDge10-P2A-mCherry |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002338 |
| 产品用途: | A human codon-optimized ISDge10 TnpB and chimeric guide RNA expression plasmid. |
| 存储实验室: | Guanghai Xiang lab |
|
载体骨架基本信息 |
|
| 载体名称: | px330 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CAG |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Dh5a |
|
插入基因信息 |
|
| 插入基因名称: | Nuclease, mCherry |
| 物种来源: | Synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Comprehensive assessment of activity, specificity, and safety of hypercompact TnpB systems for gene editing.
Xin C., Xiang G., Cao S., Wang Y., Yuan S., Liu X., Huo Y., Sun J., Wan X., Liu D., Hong J., Hu J., Wang H.
Genome Biol. 2026 (2026)
|
px330-U6-ISDge10 gRNA(PciI XbaI)-CAG-ISDge10-P2A-mCherry
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
px330-U6-ISDge10 gRNA(PciI XbaI)-CAG-ISDge10-P2A-mCherry载体质粒基本信息
px330-U6-ISDge10 gRNA(PciI XbaI)-CAG-ISDge10-P2A-mCherry质粒图谱载体图谱和px330-U6-ISDge10 gRNA(PciI XbaI)-CAG-ISDge10-P2A-mCherry载体序列质粒序列多克隆位点信息
px330-U6-ISDge10 gRNA(PciI XbaI)-CAG-ISDge10-P2A-mCherry质粒载体简介
px330-U6-ISDge10 gRNA(PciI XbaI)-CAG-ISDge10-P2A-mCherry质粒序列载体序列
LOCUS px330-U6-ISDge10 gRNA(PciI XbaI)-CAG-ISDge10-P2A-mCherry 6345 bp DNA circular SYN 20-APR-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6345)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Aug 26, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6345
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin 1..589
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
promoter 669..917
/label=U6 promoter
/note="RNA polymerase III promoter for human U6 snRNA
(Domitrovich & Kunkel, 2003)"
enhancer 1145..1430
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer;
contains an 18-bp deletion relative to the standard CMV
enhancer"
promoter 1432..1708
/label=chicken beta-actin promoter
/note="contains a 21-bp deletion relative to the standard
chicken beta-actin promoter"
promoter 1553..1572
/label=chicken beta-actin promoter
/note="contains a 21-bp deletion relative to the standard
chicken beta-actin promoter"
intron 1709..1936
/label=hybrid intron
/note="hybrid between chicken beta-actin (CBA) and minute
virus of mice (MMV) introns (Gray et al., 2011)"
regulatory 1948..1957
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 1954..3186
/codon_start=1
/label=HIS44
/translation="MAPKKKRKVGIHGVPAATSTTHRKVYRYRIEPTPVQESKLYMLAG
SRRFVFNWALARRREHYAETGKTLGYNAQAGELTALKNQEETSWLKESDSQLLQQALKD
VERAFVNFFEKRARFPRFKSKKTDTPRFRIPQRVRIEGSRVYVPKVGWVKLRKSQEIEG
KTKSATFKREADGHWYVLLVSEFEMPDVPLPPVPESEVVGIDLGLKDFYVLSDGGRKEA
PRFARKGQRKLRRAARRHSKCTRGSNRKAKAKRKLARVHRQIANQRKDFVHKATSGLVQ
QYQGFCIENLSIKGMAKTKLSKSVLDAALGEFRRQLAYKAQWHRKWLAVIDRWFPSSKL
CGECGSINADLTLSDREWTCECGAVHDRDLNAARNIKREGLSQIVVAGHAETLNARGEG
VRPAIAGSPRGS"
CDS 1960..1980
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
misc_feature 2612..2617
/label=SDS
CDS 3187..3234
/codon_start=1
/product="bipartite nuclear localization signal from
nucleoplasmin"
/label=nucleoplasmin NLS
/translation="KRPAATKKAGQAKKKK"
CDS 3244..3300
/codon_start=1
/product="2A peptide from porcine teschovirus-1
polyprotein"
/label=P2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="ATNFSLLKQAGDVEENPGP"
CDS 3301..4011
/codon_start=1
/product="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
/label=mCherry
/note="mammalian codon-optimized"
/translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
DDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
EGRHSTGGMDELYK"
misc_feature 3317..3322
/label=SDS
misc_feature 3749..3754
/label=SDS
polyA_signal 4042..4249
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
repeat_region 4258..4398
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4258..4387
/label=AAV2 ITR
rep_origin 4473..4928
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5210..5314
/gene="bla"
/label=AmpR promoter
CDS 5315..6175
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
ORIGIN
1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt ctcgaggcta
661 gcacgcgtga gggcctattt cccatgattc cttcatattt gcatatacga tacaaggctg
721 ttagagagat aattggaatt aatttgactg taaacacaaa gatattagta caaaatacgt
781 gacgtagaaa gtaataattt cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg
841 actatcatat gcttaccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
901 ggaaaggacg aaacaccgtg gagcggttca cgaccgcgac ctcaacgccg cccggaacat
961 caagcgggaa gggctttcgc aaatcgtcgt cgcggggcac gcggagacgt taaacgctcg
1021 gggagagggt gtcagacctg cgatagcggg cagccctcga tgaagcgaga atccaacggc
1081 tttagccgtt ggagtgtcaa ccaggtctgc atgtcttacc ttttttaagc ttgtcgaagg
1141 tacccgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
1201 cattgacgtc aatagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
1261 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
1321 ttgacgtcaa tgacggtaaa tggcccgcct ggcattgtgc ccagtacatg accttatggg
1381 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga
1441 gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt
1501 tatttatttt ttaattattt tgtgcagcga tgggggcggg gggggggggg ggcgcgcgcc
1561 aggcggggcg gggcggggcg aggggcgggg cggggcgagg cggagaggtg cggcggcagc
1621 caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg aggcggcggc ggcggcggcc
1681 ctataaaaag cgaagcgcgc ggcgggcggg agtcgctgcg cgctgccttc gccccgtgcc
1741 ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt tactcccaca
1801 ggtgagcggg cgggacggcc cttctcctcc gggctgtaat tagctgagca agaggtaagg
1861 gtttaaggga tggttggttg gtggggtatt aatgtttaat tacctggagc acctgcctga
1921 aatcactttt tttcaggttg gaccggtgcc accatggccc caaagaagaa gcggaaggtc
1981 ggtatccacg gagtcccagc agccactagt accacccacc gcaaggtgta cagataccgc
2041 atcgagccca cccccgtgca ggagagcaag ctgtacatgc tggccggcag ccggagattc
2101 gtgttcaact gggccctggc ccggagaaga gagcactacg ccgaaaccgg caagaccctg
2161 ggatacaacg cccaggcagg agagctgacc gccctgaaaa accaagaaga aaccagctgg
2221 ctgaaagagt ccgactcaca actgctgcag caggcactga aagacgtgga aagagccttc
2281 gtgaacttct tcgaaaaacg ggccagattc cccagattca aaagcaagaa aaccgacacc
2341 cccagattca gaatccccca aagagtgaga atagagggca gcagagtgta cgtgcccaag
2401 gtggggtggg tgaagctgag aaagagtcag gagatagaag gaaaaaccaa aagcgccacc
2461 ttcaagaggg aggcagatgg ccattggtac gtgctgctgg tgagtgagtt cgagatgcct
2521 gatgtgcctc tgcccccagt gcccgagagc gaggtggtgg gaatcgacct gggactgaag
2581 gacttctacg tcctgtctga cgggggcagg aaggaggccc ccagattcgc tagaaagggc
2641 cagcgaaaac tgagaagagc tgcaagaaga catagcaagt gcacccgggg aagcaacaga
2701 aaggccaagg caaagaggaa gctggcaaga gtgcacagac agatcgcaaa ccagagaaaa
2761 gacttcgtgc acaaggccac cagcggcctc gtgcagcagt accaaggctt ctgcatcgaa
2821 aacctgagca tcaagggcat ggccaaaacc aaactgtcaa aaagcgtgct ggacgcagcc
2881 ctgggggaat ttagaagaca gctggcctat aaagcccagt ggcatagaaa gtggctggca
2941 gtgatagaca gatggttccc cagcagcaag ctgtgcggag agtgcggcag cattaacgcc
3001 gatctgaccc tgagcgacag agaatggacc tgcgagtgtg gcgcagtgca cgacagagat
3061 ctgaacgccg ctcgcaacat caagagagag ggcctgagtc agattgtggt ggccggacat
3121 gccgaaaccc tgaacgccag aggggagggc gtgagacccg ccattgccgg aagccctaga
3181 ggatccaaaa ggccggcggc cacgaaaaag gccggccagg caaaaaagaa aaagggaagc
3241 ggagctacta acttcagcct gctgaagcag gctggagacg tggaggagaa ccctggacct
3301 atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag
3361 gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc
3421 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc
3481 ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac
3541 cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc
3601 gtgatgaact tcgatgacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac
3661 ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta
3721 atgcagaaga aaaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc
3781 gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct
3841 gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc
3901 aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa
3961 cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagta agaattccta
4021 gagctcgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
4081 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
4141 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
4201 aggacagcaa gggggaggat tgggaagaga atagcaggca tgctggggag cggccgcagg
4261 aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg
4321 ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag
4381 cgcgcagctg cctgcagggg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
4441 tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg cattaagcgc
4501 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgcct tagcgcccgc
4561 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
4621 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
4681 acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
4741 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
4801 caactctatc tcgggctatt cttttgattt ataagggatt ttgccgattt cggtctattg
4861 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt
4921 tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
4981 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
5041 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
5101 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
5161 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
5221 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
5281 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
5341 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
5401 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
5461 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
5521 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
5581 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
5641 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
5701 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
5761 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
5821 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
5881 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
5941 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
6001 tgctgataaa tctggagccg gtgagcgtgg aagccgcggt atcattgcag cactggggcc
6061 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
6121 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
6181 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
6241 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
6301 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttc
//
