| 质粒名称: | pAAVLINKv2-SCP1-Cre-UDeg3a-3'-SCN8A-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001421 |
| 产品用途: | 3' AAVLINK plasmid for SCN8A expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | SCP1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | SCN8A |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-SCN8A-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-SCN8A-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-SCN8A-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-SCN8A-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-SCN8A-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-3'-SCN8A-FLAG 7283 bp DNA circular SYN 11-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7283)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 7, 2026 from SnapGene 6.0.2
https:///www.biofeng.com
FEATURES Location/Qualifiers
source 1..7283
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1547..3996
/codon_start=1
/label=SCN8A
/translation="VVSSGSSAARSTSRKG*ASLGGSCGKPASSSWSTTGLRPSSSS*F
C*AVAPWPSRTSTLSRERPSAPSWNMLTKSSPISSSWRCCSSGQPMASSSSSPMPGVGW
TSSLWLSL*SAL*LMPWATRN*VP*SPLGP*EL*DP*EPYHDLKG*GWW*MPWWAPSPP
S*MCCWCVSSSG*FSASWELTCLRESTTTALMRLLKSDLKLKMSTIKLNVKSLWRGTIQ
RSDGRT*RSTLTMLGQDTWPFFK*QPSKAGWTSCMQL*IPGSLMSSLSMRTISTCTSIL
SSSSSSAPSSP*TCSLVSSLITSINKRKSSEVRTSS*PKNRRSTTMP*KSWAQRSHRNL
FPAP*TKSKESSLILSLSKPLTLLS*CSSALTW*Q*WWRQTLKASRWRTSSTGLTWCLL
SSSPVSVCSKCLR*GTTTSPLAGTSSTSW*SSSPLWECSWQI*LRNTLFPQPYSESSDW
PVLGASCV*SKAPKGFVPCSLP**CPCLPCSTSAFCSSWSCSSSPFLGCPILHM*STRL
VSMTCSTLRHLATA*SACFKSQPQLVGMACCCPS*TAPLTAA*IRNTQGVALREIVGTP
QWASSSL*ATSSSLS*LS*TCTLPSSWRTSV*PQRKVQTL*VRMTLRPSMRSGRSSTPM
PPSSLSTVSWQTLQMPWSILSECPSPIPLSSSLWICQW*AGIASTAWTSFLPSPSGSWE
IAGSWTSCGSRWKSGSWHPILPKCLTSQSQPHCVASRRRYLQWSCSVPTGDIWQGGASS
AKRQLLISWRMEAHTGRKKRAPHLQPPSRPMTV*LNLKRRNSSGQRKEEGKEPKDKKRS
ENPS"
misc_feature 3737..3742
/label=SDS
misc_feature 3740..3745
/label=SDS
CDS 4003..4068
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 4072..4503
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 4216..4221
/label=SDS
repeat_region 4546..4686
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4546..4675
/label=AAV2 ITR
rep_origin 4761..5216
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5498..5602
/gene="bla"
/label=AmpR promoter
CDS 5603..6463
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6634..7222
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggttg tgtccagcgg
1561 ttcaagtgct gccaggtcaa catcgaggaa gggctaggca agtcttggtg gatcctgcgg
1621 aaaacctgct tcctcatcgt ggagcacaac tggtttgaga ccttcatcat cttcatgatt
1681 ctgctgagca gtggcgccct ggccttcgag gacatctaca ttgagcagag aaagaccatc
1741 cgcaccatcc tggaatatgc tgacaaagtc ttcacctata tcttcatcct ggagatgttg
1801 ctcaagtgga cagcctatgg cttcgtcaag ttcttcacca atgcctggtg ttggctggac
1861 ttcctcattg tggctgtctc tttagtcagc cttatagcta atgccctggg ctactcggaa
1921 ctaggtgcca taaagtccct taggacccta agagctttga gacccttaag agccttatca
1981 cgatttgaag ggatgagggt ggtggtgaat gccttggtgg gcgccatccc ctccatcatg
2041 aatgtgctgc tggtgtgtct catcttctgg ctgattttca gcatcatggg agttaacttg
2101 tttgcgggaa agtaccacta ctgctttaat gagacttctg aaatccgatt tgaaattgaa
2161 gatgtcaaca ataaaactga atgtgaaaag cttatggagg ggaacaatac agagatcaga
2221 tggaagaacg tgaagatcaa ctttgacaat gttggggcag gatacctggc ccttcttcaa
2281 gtagcaacct tcaaaggctg gatggacatc atgtatgcag ctgtagattc ccggaagcct
2341 gatgagcagc ctaagtatga ggacaatatc tacatgtaca tctattttgt catcttcatc
2401 atcttcggct ccttcttcac cctgaacctg ttcattggtg tcatcattga taacttcaat
2461 caacaaaaga aaaagttcgg aggtcaggac atcttcatga ccgaagaaca gaagaagtac
2521 tacaatgcca tgaaaaagct gggctcaaag aagccacaga aacctattcc ccgccccttg
2581 aacaaaatcc aaggaatcgt ctttgatttt gtcactcagc aagcctttga cattgttatc
2641 atgatgctca tctgccttaa catggtgaca atgatggtgg agacagacac tcaaagcaag
2701 cagatggaga acatcctcta ctggattaac ctggtgtttg ttatcttctt cacctgtgag
2761 tgtgtgctca aaatgtttgc gttgaggcac tactacttca ccattggctg gaacatcttc
2821 gacttcgtgg tagtcatcct ctccattgtg ggaatgttcc tggcagatat aattgagaaa
2881 tactttgttt ccccaaccct attccgagtc atccgattgg cccgtattgg gcgcatcttg
2941 cgtctgatca aaggcgccaa agggattcgt accctgctct ttgccttaat gatgtccttg
3001 cctgccctgt tcaacatcgg ccttctgctc ttcctggtca tgttcatctt ctccattttt
3061 gggatgtcca attttgcata tgtgaagcac gaggctggta tcgatgacat gttcaacttt
3121 gagacatttg gcaacagcat gatctgcctg tttcaaatca caacctcagc tggttgggat
3181 ggcctgctgc tgcccatcct aaaccgcccc cctgactgca gcctagataa ggaacaccca
3241 gggagtggct ttaagggaga ttgtgggaac ccctcagtgg gcatcttctt ctttgtaagc
3301 tacatcatca tctctttcct aattgtcgtg aacatgtaca ttgccatcat cctggagaac
3361 ttcagtgtag ccacagagga aagtgcagac cctctgagtg aggatgactt tgagaccttc
3421 tatgagatct gggagaagtt cgaccccgat gccacccagt tcattgagta ctgtaagctg
3481 gcagactttg cagatgcctt ggagcatcct ctccgagtgc ccaagcccaa taccattgag
3541 ctcatcgcta tggatctgcc aatggtgagc ggggatcgca tccactgctt ggacatcctt
3601 tttgccttca ccaagcgggt cctgggagat agcggggagt tggacatcct gcggcagcag
3661 atggaagagc ggttcgtggc atccaatcct tccaaagtgt cttacgagcc aatcacaacc
3721 acactgcgtc gcaagcagga ggaggtatct gcagtggtcc tgcagcgtgc ctaccgggga
3781 catttggcaa ggcggggctt catctgcaaa aagacaactt ctaataagct ggagaatgga
3841 ggcacacacc gggagaaaaa agagagcacc ccatctacag cctccctccc gtcctatgac
3901 agtgtaacta aacctgaaaa ggagaaacag cagcgggcag aggaaggaag aagggaaaga
3961 gccaaaagac aaaaagaggt cagagaatcc aagtgttgta cagactacaa agaccatgac
4021 ggtgattata aagatcatga catcgactac aaggatgacg atgacaagta actcgagata
4081 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
4141 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
4201 tggctttcat tttctcctcc ttgtataaat cctggttagt tcttgccacg gcggaactca
4261 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
4321 tggtgtttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatc tagctttatt
4381 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
4441 aacaacaaca attgcattca ttttatgttt caggttcagg gggagatgtg ggaggttttt
4501 taaagatctg gagaccggtg tggcacgtgc ggaccgagcg gccgcaggaa cccctagtga
4561 tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg
4621 tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc
4681 tgcaggggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
4741 tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
4801 ggttacgcgc agcgtgaccg ctacacttgc cagcgcctta gcgcccgctc ctttcgcttt
4861 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
4921 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg
4981 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
5041 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca actctatctc
5101 gggctattct tttgatttat aagggatttt gccgatttcg gtctattggt taaaaaatga
5161 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta caattttatg
5221 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc
5281 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
5341 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
5401 gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
5461 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
5521 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
5581 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
5641 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
5701 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
5761 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
5821 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
5881 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
5941 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
6001 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
6061 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
6121 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
6181 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
6241 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
6301 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
6361 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
6421 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
6481 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
6541 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
6601 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
6661 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
6721 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
6781 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
6841 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
6901 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
6961 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
7021 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
7081 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
7141 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
7201 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
7261 ttgctggcct tttgctcaca tgt
//
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-SCN8A-FLAG载体图谱质粒图谱pdf版和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-SCN8A-FLAG载体序列质粒序列等相关资料下载
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-SCN8A-FLAG质粒载体应用举例
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