pAAVLINKv2-SCP1-Cre-UDeg3a-3'-MSH6-FLAG

LOCUS       pAAVLINKv2-SCP1-Cre-UDeg3a-3'-MSH6-FLAG             7055 bp DNA     circular SYN 18-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7055)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported May 22, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7055
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    12..130
                     /label=AAV2 ITR
     misc_feature    145..225
                     /label=SCP1
     CDS             237..1265
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     CDS             1281..1370
                     /codon_start=1
                     /label=UDeg3a
                     /translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
     misc_feature    1295..1300
                     /label=SDS
     misc_feature    1298..1303
                     /label=SDS
     intron          1374..1414
                     /label=Splicing Donor
     protein_bind    1421..1454
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1455..1546
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1547..3768
                     /codon_start=1
                     /label=MSH6
                     /translation="V*YPAPSFGMHPKL*ELSLRKNILGKS*VMALG*CYPRCLKV*LQ
                     SLIPLG*HQERKVNWPSLL*VVVSSTSKNALLIRSFYQWLILKNIFPWILTQSALQDLV
                     LSSPKPINEWC*MQ*H*TTWRFF*MEQMVLLKEPY*RGLILAILLLVSGS*SNGFVPHS
                     VTIMLLMIV*MP*KTSWLCLTKSPKL*SF*RSFQILRGYSVKFIMLGLP*RVRTTQTAG
                     L*CMKKLHTARRRLLIFFLLWKDSK*CVKL*GSWKKLLMVLSLKSLSRSSLCRQKILKV
                     VFLI*L*N*TDGIQPLTMKRLERLDLLLPKQALTLIMTKLLLT*EKMNRASWNT*RNSA
                     TELAVGP*SIGGLVGTVTSWKFLRISPLAICQKNTS*NLPRRAVNDTGPKLLKRSWLIS
                     *MLKNGGMYH*RTACGDCSITLIKITRTGSLL*SVSQCWMFYCAWLTIVEGVMVLCVAQ
                     *FCCRKIPPPS*SLKDHAILALRRLFLEMILFLMTF**AVRKRSRKMAKPIVCLLLDQI
                     WGASLRL*DRLAY*L*WPRWVVTSLLKCAGSHQLIECLLDLVPQTE*CQVKVHFLLN*V
                     KLPAYSCMQQHILWCLWMN*EEVLQHLMGRQ*QMQLLKNLLRL*NVVHYFQLTTIH**K
                     IILKMLLCA*DIWHAW*KMNVKTPARRLLRSSINSLRELVLKAMALMQQGLLISQRKLF
                     KRDIEKQENLRR*ISHYDYFGKFAWLVKGQL*MLKLSINC*L*LRN"
     misc_feature    2538..2543
                     /label=SDS
     CDS             3775..3840
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      3844..4275
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    3988..3993
                     /label=SDS
     repeat_region   4318..4458
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4318..4447
                     /label=AAV2 ITR
     rep_origin      4533..4988
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5270..5374
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5375..6235
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6406..6994
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
      181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
      241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
      301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
      361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
      421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
      481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
      541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
      601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
      661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
      721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
      781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
      841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
      901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
      961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
     1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
     1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
     1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
     1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
     1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
     1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
     1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
     1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
     1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggtct gatacccggc
     1561 tcccagtttt gggatgcatc caaaactttg agaactctcc ttgaggaaga atattttagg
     1621 gaaaagctaa gtgatggcat tggggtgatg ttaccccagg tgcttaaagg tatgacttca
     1681 gagtctgatt ccattgggtt gacaccagga gagaaaagtg aattggccct ctctgctcta
     1741 ggtggttgtg tcttctacct caaaaaatgc cttattgatc aggagctttt atcaatggct
     1801 aattttgaag aatatattcc cttggattct gacacagtca gcactacaag atctggtgct
     1861 atcttcacca aagcctatca acgaatggtg ctagatgcag tgacattaaa caacttggag
     1921 atttttctga atggaacaaa tggttctact gaaggaaccc tactagagag ggttgatact
     1981 tgccatactc cttttggtaa gcggctccta aagcaatggc tttgtgcccc actctgtaac
     2041 cattatgcta ttaatgatcg tctagatgcc atagaagacc tcatggttgt gcctgacaaa
     2101 atctccgaag ttgtagagct tctaaagaag cttccagatc ttgagaggct actcagtaaa
     2161 attcataatg ttgggtctcc cctgaagagt cagaaccacc cagacagcag ggctataatg
     2221 tatgaagaaa ctacatacag caagaagaag attattgatt ttctttctgc tctggaagga
     2281 ttcaaagtaa tgtgtaaaat tatagggatc atggaagaag ttgctgatgg ttttaagtct
     2341 aaaatcctta agcaggtcat ctctctgcag acaaaaaatc ctgaaggtcg ttttcctgat
     2401 ttgactgtag aattgaaccg atgggataca gcctttgacc atgaaaaggc tcgaaagact
     2461 ggacttatta ctcccaaagc aggctttgac tctgattatg accaagctct tgctgacata
     2521 agagaaaatg aacagagcct cctggaatac ctagagaaac agcgcaacag aattggctgt
     2581 aggaccatag tctattgggg gattggtagg aaccgttacc agctggaaat tcctgagaat
     2641 ttcaccactc gcaatttgcc agaagaatac gagttgaaat ctaccaagaa gggctgtaaa
     2701 cgatactgga ccaaaactat tgaaaagaag ttggctaatc tcataaatgc tgaagaacgg
     2761 agggatgtat cattgaagga ctgcatgcgg cgactgttct ataactttga taaaaattac
     2821 aaggactggc agtctgctgt agagtgtatc gcagtgttgg atgttttact gtgcctggct
     2881 aactatagtc gagggggtga tggtcctatg tgtcgcccag taattctgtt gccggaagat
     2941 acccccccct tcttagagct taaaggatca cgccatcctt gcattacgaa gacttttttt
     3001 ggagatgatt ttattcctaa tgacattcta ataggctgtg aggaagagga gcaggaaaat
     3061 ggcaaagcct attgtgtgct tgttactgga ccaaatatgg ggggcaagtc tacgcttatg
     3121 agacaggctg gcttattagc tgtaatggcc cagatgggtt gttacgtccc tgctgaagtg
     3181 tgcaggctca caccaattga tagagtgttt actagacttg gtgcctcaga cagaataatg
     3241 tcaggtgaaa gtacattttt tgttgaatta agtgaaactg ccagcatact catgcatgca
     3301 acagcacatt ctctggtgct tgtggatgaa ttaggaagag gtactgcaac atttgatggg
     3361 acggcaatag caaatgcagt tgttaaagaa cttgctgaga ctataaaatg tcgtacatta
     3421 ttttcaactc actaccattc attagtagaa gattattctc aaaatgttgc tgtgcgccta
     3481 ggacatatgg catgcatggt agaaaatgaa tgtgaagacc ccagccagga gactattacg
     3541 ttcctctata aattcattaa gggagcttgt cctaaaagct atggctttaa tgcagcaagg
     3601 cttgctaatc tcccagagga agttattcaa aagggacata gaaaagcaag agaatttgag
     3661 aagatgaatc agtcactacg attatttcgg gaagtttgcc tggctagtga aaggtcaact
     3721 gtagatgctg aagctgtcca taaattgctg actttgatta aggaattatg tacagactac
     3781 aaagaccatg acggtgatta taaagatcat gacatcgact acaaggatga cgatgacaag
     3841 taactcgaga taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     3901 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     3961 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggtta gttcttgcca
     4021 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     4081 ctgacaattc cgtggtgttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
     4141 tctagcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
     4201 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggagatg
     4261 tgggaggttt tttaaagatc tggagaccgg tgtggcacgt gcggaccgag cggccgcagg
     4321 aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg
     4381 ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag
     4441 cgcgcagctg cctgcagggg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
     4501 tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg cattaagcgc
     4561 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgcct tagcgcccgc
     4621 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     4681 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     4741 acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     4801 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     4861 caactctatc tcgggctatt cttttgattt ataagggatt ttgccgattt cggtctattg
     4921 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt
     4981 tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
     5041 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     5101 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     5161 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     5221 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     5281 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     5341 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     5401 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     5461 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     5521 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     5581 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     5641 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     5701 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     5761 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     5821 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     5881 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     5941 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     6001 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     6061 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     6121 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     6181 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     6241 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     6301 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     6361 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     6421 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     6481 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     6541 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     6601 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     6661 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     6721 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     6781 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     6841 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     6901 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     6961 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     7021 gttcctggcc ttttgctggc cttttgctca catgt
//