pAAVLINKv2-SCP1-Cre-UDeg3a-3'-AGL-FLAG

LOCUS       pAAVLINKv2-SCP1-Cre-UDeg3a-3'-AGL-FLAG         6950 bp DNA     circular SYN 18-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6950)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported May 22, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6950
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    12..130
                     /label=AAV2 ITR
     misc_feature    145..225
                     /label=SCP1
     CDS             237..1265
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     CDS             1281..1370
                     /codon_start=1
                     /label=UDeg3a
                     /translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
     misc_feature    1295..1300
                     /label=SDS
     misc_feature    1298..1303
                     /label=SDS
     intron          1374..1414
                     /label=Splicing Donor
     protein_bind    1421..1454
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1455..1546
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1547..3663
                     /codon_start=1
                     /label=AGL
                     /translation="GPMNIFKK*NLKTCLQEVLLYSELVLIHMHKSLLEFFEII*HNSV
                     LTLNLAA*LLTMQILY*KFLLLLLPPD*LWLS*IRSFTDVNQKKRKMVEGAMTYQTGQP
                     LNMQVFKV*CLYWQK*DQRMTWGILFVII*DLEIG*LTMSVTGLFHDQELLLKLVNGCR
                     LCSST*SRSHVTLSHVTLMLY*LVHIPLFWIQHGSRCQALFRMVQPL*NTFHWVQFNCV
                     E*ENSLPCQFFHLP*WMYLIG*MRSQKKRSNVVFL*LQAYLIFLLVFSAAGEGILLLHL
                     EVYC*LLDAM*KPGILF*HLRVP*GMVSFLIYWVKEFMPDTIVGMLCGGGCSVSRITVK
                     WFQMV*TFSSAQFPECILQMILLLCLLAHWISHCLKSYRKQCKNTCRAYSSEKGMLVPR
                     *IET*RTKVLI*LQELMKKQDLFMEEIVSIVAHGWIKWEKVTELETEESQPHQEMGLLW
                     KLWA*VNLLFAGCWNYPKKIFSLIMKSQ*KDMERL*RSHMMSGTEKYKTTLKSYFMFPK
                     TLQI*MKSIQIWFTNVAYTKIVMELQVLGVTISSGLILP*QWLWPLSSLLQKKHGKLWR
                     LQKKNCLVPLA*KL*IQMIWFTVEFMTMH*TMTTTILLKVSIITKDLSGCGLLGIFFVQ
                     NYIFPD*WARRLLQRL*FWLKMFFPDIMFILRDPLGKDFQN*PMRMPSTVLSAVKHKPG
                     QLLLFLRHFMI"
     CDS             3670..3735
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      3739..4170
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    3883..3888
                     /label=SDS
     repeat_region   4213..4353
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4213..4342
                     /label=AAV2 ITR
     rep_origin      4428..4883
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5165..5269
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5270..6130
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6301..6889
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
      181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
      241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
      301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
      361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
      421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
      481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
      541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
      601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
      661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
      721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
      781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
      841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
      901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
      961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
     1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
     1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
     1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
     1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
     1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
     1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
     1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
     1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
     1501 gcacctattg gtcttactga catccacttt gcctttctct ccacagggcc caatgaatat
     1561 attcaagaaa tagaatttga aaacttgtct ccaggaagtg ttattatatt cagagttagt
     1621 cttgatccac atgcacaagt cgctgttgga attcttcgaa atcatctgac acaattcagt
     1681 cctcacttta aatctggcag cctagctgtt gacaatgcag atcctatatt aaaaattcct
     1741 tttgcttctc ttgcctccag attaactttg gctgagctaa atcagatcct ttaccgatgt
     1801 gaatcagaag aaaaggaaga tggtggaggg tgctatgaca taccaaactg gtcagccctt
     1861 aaatatgcag gtcttcaagg tttaatgtct gtattggcag aaataagacc aaagaatgac
     1921 ttggggcatc ctttttgtaa taatttgaga tctggagatt ggatgattga ctatgtcagt
     1981 aaccggctta tttcacgatc aggaactatt gctgaagttg gtaaatggtt gcaggctatg
     2041 ttcttctacc tgaagcagat cccacgttac cttatcccat gttactttga tgctatatta
     2101 attggtgcat ataccactct tctggataca gcatggaagc agatgtcaag ctttgttcag
     2161 aatggttcaa cctttgtgaa acacctttca ttgggttcag ttcaactgtg tggagtagga
     2221 aaattccctt ccctgccaat tctttcacct gccctaatgg atgtacctta taggttaaat
     2281 gagatcacaa aagaaaagga gcaatgttgt gtttctctag ctgcaggctt acctcatttt
     2341 tcttctggta ttttccgctg ctggggaagg gatactttta ttgcacttag aggtatactg
     2401 ctgattactg gacgctatgt agaagccagg aatattattt tagcatttgc gggtaccctg
     2461 aggcatggtc tcattcctaa tctactgggt gaaggaattt atgccagata caattgtcgg
     2521 gatgctgtgt ggtggtggct gcagtgtatc caggattact gtaaaatggt tccaaatggt
     2581 ctagacattc tcaagtgccc agtttccaga atgtatccta cagatgattc tgctcctttg
     2641 cctgctggca cactggatca gccattgttt gaagtcatac aggaagcaat gcaaaaacac
     2701 atgcagggca tacagttccg agaaaggaat gctggtcccc agatagatcg aaacatgaag
     2761 gacgaaggtt ttaatataac tgcaggagtt gatgaagaaa caggatttgt ttatggagga
     2821 aatcgtttca attgtggcac atggatggat aaaatgggag aaagtgacag agctagaaac
     2881 agaggaatcc cagccacacc aagagatggg tctgctgtgg aaattgtggg cctgagtaaa
     2941 tctgctgttc gctggttgct ggaattatcc aaaaaaaata ttttccctta tcatgaagtc
     3001 acagtaaaaa gacatggaaa ggctataaag gtctcatatg atgagtggaa cagaaaaata
     3061 caagacaact ttgaaaagct atttcatgtt tccgaagacc cttcagattt aaatgaaaag
     3121 catccaaatc tggttcacaa acgtggcata tacaaagata gttatggagc ttcaagtcct
     3181 tggtgtgact atcagctcag gcctaatttt accatagcaa tggttgtggc ccctgagctc
     3241 tttactacag aaaaagcatg gaaagctttg gagattgcag aaaaaaaatt gcttggtccc
     3301 cttggcatga aaactttaga tccagatgat atggtttact gtggaattta tgacaatgca
     3361 ttagacaatg acaactacaa tcttgctaaa ggtttcaatt atcaccaagg acctgagtgg
     3421 ctgtggccta ttgggtattt tcttcgtgca aaattatatt tttccagatt gatgggcccg
     3481 gagactactg caaagactat agttttggtt aaaaatgttc tttcccgaca ttatgttcat
     3541 cttgagagat ccccttggaa aggacttcca gaactgacca atgagaatgc ccagtactgt
     3601 cctttcagct gtgaaacaca agcctggtca attgctacta ttcttgagac actttatgat
     3661 ttatgtacag actacaaaga ccatgacggt gattataaag atcatgacat cgactacaag
     3721 gatgacgatg acaagtaact cgagataatc aacctctgga ttacaaaatt tgtgaaagat
     3781 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     3841 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     3901 ggttagttct tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     3961 ctcggctgtt gggcactgac aattccgtgg tgtttatttg tgaaatttgt gatgctattg
     4021 ctttatttgt aaccatctag ctttatttgt gaaatttgtg atgctattgc tttatttgta
     4081 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag
     4141 gttcaggggg agatgtggga ggttttttaa agatctggag accggtgtgg cacgtgcgga
     4201 ccgagcggcc gcaggaaccc ctagtgatgg agttggccac tccctctctg cgcgctcgct
     4261 cgctcactga ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct
     4321 cagtgagcga gcgagcgcgc agctgcctgc aggggcgcct gatgcggtat tttctcctta
     4381 cgcatctgtg cggtatttca caccgcatac gtcaaagcaa ccatagtacg cgccctgtag
     4441 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     4501 cgccttagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     4561 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     4621 cctcgacccc aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata
     4681 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     4741 aactggaaca acactcaact ctatctcggg ctattctttt gatttataag ggattttgcc
     4801 gatttcggtc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     4861 caaaatatta acgtttacaa ttttatggtg cactctcagt acaatctgct ctgatgccgc
     4921 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     4981 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     5041 gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt
     5101 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     5161 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     5221 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     5281 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     5341 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     5401 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     5461 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     5521 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     5581 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     5641 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     5701 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     5761 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     5821 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     5881 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     5941 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     6001 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     6061 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     6121 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     6181 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     6241 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     6301 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     6361 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     6421 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     6481 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     6541 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     6601 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     6661 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     6721 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     6781 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     6841 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     6901 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
//