| 质粒名称: | pAAVLINKv1-pCALM1-Cre-3'-EIF4G1-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001630 |
| 产品用途: | 3' AAVLINK plasmid for EIF4G1 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | EIF4G1 |
| 物种来源: |
H.sapiens
|
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv1-pCALM1-Cre-3'-EIF4G1-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv1-pCALM1-Cre-3'-EIF4G1-FLAG载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-3'-EIF4G1-FLAG质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-3'-EIF4G1-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-3'-EIF4G1-FLAG质粒载体简介
pAAVLINKv1-pCALM1-Cre-3'-EIF4G1-FLAG质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-3'-EIF4G1-FLAG 6387 bp DNA circular SYN 18-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6387)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 21, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6387
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 145..265
/label=pCALM1
CDS 277..1308
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1309..1349
/label=Splicing Donor
protein_bind 1356..1389
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1390..1481
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1482..3100
/codon_start=1
/label=EIF4G1
/translation="VAAPLTPHDSPRSPSLAPSILTTSSLHLEGD*AGARAAAEAQEPS
PQTQHQKLLAQLLVL*IASQPFNKRYPQKAQIIDVWCRGVA*AENEARKLETEETA*SG
VNGEGTVGTGLIVRGHLLPSGASARKWRSGVENGPPSLRGCARQLASRRIGTVGGMP*S
EKLPYPQ*AP*RRLSLRRS*RRNPRLSLRNISISMT*KRQSSACRSWPHPPCSSSLYGM
VSSLRWSAVPLLVSIWGSCCTSCSVLGICLLLSTTKGCMKSWNWLRTWKLTSPTCGST*
RNW*HPFCRKVGCPWGSCSGRLQSL*DRWAKLLPCCWRSWASCAKAWVLKRWGRCGEKP
GLAGRNFYLKARTLVHSSLNRRWSIPWERSRKPLARGHSPPRS*TGSWRSC*RRAAVTS
GCSTG*RPT*VSSR*YPTR*FEPS*RLSAILQLFLRLPSEWTLQC*KRERSCCRNTCVT
SRRSYRRSTPSRPL**P*NSLPTCCGCSLTHCMTRTW*RRMPSTVGRVARTPLSSRARV
WPLNLSQPSSSGSVKQRRSLTT"
misc_feature 2019..2024
/label=SDS
misc_feature 2118..2123
/label=SDS
misc_feature 2446..2451
/label=SDS
misc_feature 2658..2663
/label=SDS
misc_feature 2970..2975
/label=SDS
misc_feature 3081..3086
/label=SDS
CDS 3107..3172
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3176..3607
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3320..3325
/label=SDS
repeat_region 3650..3790
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3650..3779
/label=AAV2 ITR
rep_origin 3865..4320
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4602..4706
/gene="bla"
/label=AmpR promoter
CDS 4707..5567
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5738..6326
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
1441 tattggtctt actgacatcc actttgcctt tctctccaca ggtagccgcc ccattgacac
1501 ctcacgactc accaagatca ccaagcctgg ctccatcgat tctaacaacc agctctttgc
1561 acctggaggg cgactgagct ggggcaaggg cagcagcgga ggctcaggag ccaagccctc
1621 agacgcagca tcagaagctg ctcgcccagc tactagtact ttgaatcgct tctcagccct
1681 tcaacaagcg gtacccacag aaagcacaga taatagacgt gtggtgcaga ggagtagctt
1741 gagccgagaa cgaggcgaga aagctggaga ccgaggagac cgcctagagc ggagtgaacg
1801 gggaggggac cgtggggacc ggcttgatcg tgcgcggaca cctgctacca agcggagctt
1861 cagcaaggaa gtggaggagc ggagtagaga acggccctcc cagcctgagg ggctgcgcaa
1921 ggcagctagc ctcacggagg atcgggaccg tgggcgggat gccgtgaagc gagaagctgc
1981 cctaccccca gtgagccccc tgaaggcggc tctctctgag gaggagttag agaagaaatc
2041 caaggctatc attgaggaat atctccatct caatgacatg aaagaggcag tccagtgcgt
2101 gcaggagctg gcctcaccct ccttgctctt catctttgta cggcatggtg tcgagtctac
2161 gctggagcgc agtgccattg ctcgtgagca tatggggcag ctgctgcacc agctgctctg
2221 tgctgggcat ctgtctactg ctcagtacta ccaagggttg tatgaaatct tggaattggc
2281 tgaggacatg gaaattgaca tcccccacgt gtggctctac ctagcggaac tggtaacacc
2341 cattctgcag gaaggtgggg tgcccatggg ggagctgttc agggagatta caaagcctct
2401 gagaccgttg ggcaaagctg cttccctgtt gctggagatc ctgggcctcc tgtgcaaaag
2461 catgggtcct aaaaaggtgg ggacgctgtg gcgagaagcc gggcttagct ggaaggaatt
2521 tctacctgaa ggccaggaca ttggtgcatt cgtcgctgaa cagaaggtgg agtataccct
2581 gggagaggag tcggaagccc ctggccagag ggcactcccc tccgaggagc tgaacaggca
2641 gctggagaag ctgctgaagg agggcagcag taaccagcgg gtgttcgact ggatagaggc
2701 caacctgagt gagcagcaga tagtatccaa cacgttagtt cgagccctca tgacggctgt
2761 ctgctattct gcaattattt ttgagactcc cctccgagtg gacgttgcag tgctgaaagc
2821 gcgagcgaag ctgctgcaga aatacctgtg tgacgagcag aaggagctac aggcgctcta
2881 cgccctccag gcccttgtag tgaccttaga acagcctccc aacctgctgc ggatgttctt
2941 tgacgcactg tatgacgagg acgtggtgaa ggaggatgcc ttctacagtt gggagagtag
3001 caaggacccc gctgagcagc agggcaaggg tgtggccctt aaatctgtca cagccttctt
3061 caagtggctc cgtgaagcag aggaggagtc tgaccacaac tgtacagact acaaagacca
3121 tgacggtgat tataaagatc atgacatcga ctacaaggat gacgatgaca agtaactcga
3181 gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
3241 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
3301 cgtatggctt tcattttctc ctccttgtat aaatcctggt tagttcttgc cacggcggaa
3361 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
3421 tccgtggtgt ttatttgtga aatttgtgat gctattgctt tatttgtaac catctagctt
3481 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca
3541 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggaga tgtgggaggt
3601 tttttaaaga tctggagacc ggtgtggcac gtgcggaccg agcggccgca ggaaccccta
3661 gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca
3721 aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc
3781 tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
3841 cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg
3901 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cttagcgccc gctcctttcg
3961 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
4021 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
4081 tgggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
4141 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaactcta
4201 tctcgggcta ttcttttgat ttataaggga ttttgccgat ttcggtctat tggttaaaaa
4261 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt
4321 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc
4381 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
4441 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
4501 gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
4561 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
4621 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
4681 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
4741 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
4801 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
4861 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
4921 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
4981 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
5041 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
5101 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
5161 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
5221 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
5281 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
5341 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
5401 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
5461 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
5521 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
5581 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
5641 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
5701 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
5761 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
5821 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
5881 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
5941 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
6001 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
6061 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
6121 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
6181 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
6241 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
6301 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
6361 ccttttgctg gccttttgct cacatgt
//
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