| 质粒名称: | pAAVLINK-EF1a-HA-5'-YTHDC2 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001467 |
| 产品用途: | 5' AAVLINK plasmid for YTHDC2 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | YTHDC2 |
| 物种来源: |
H.sapiens
|
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINK-EF1a-HA-5'-YTHDC2载体质粒基本信息
pAAVLINK-EF1a-HA-5'-YTHDC2质粒图谱载体图谱和pAAVLINK-EF1a-HA-5'-YTHDC2载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-HA-5'-YTHDC2质粒载体简介
pAAVLINK-EF1a-HA-5'-YTHDC2质粒序列载体序列
LOCUS pAAVLINK-EF1a-HA-5'-YTHDC2 5814 bp DNA circular SYN 14-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5814)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 11, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5814
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 146..688
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 175..386
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 399..667
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 493..498
/label=SDS
CDS 704..730
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 737..2954
/codon_start=1
/label=YTHDC2
/translation="SRPSSVSPRQPAPGGGGGGGPSPCGPGGGGRAKGLKDIRIDEEVK
IAVNIALERFRYGDQREMEFPSSLTSTERAFIHRLSQSLGLVSKSKGKGANRYLTVKKK
DGSETAHAMMTCNLTHNTKHAVRSLIQRFPVTNKERTELLPKTERGNVFAVEAENREMS
KTSGRLNNGIPQIPVKRGESEFDSFRQSLPVFEKQEEIVKIIKENKVVLIVGETGSGKT
TQIPQFLLDDCFKNGIPCRIFCTQPRRLAAIAVAERVAAERRERIGQTIGYQIRLESRV
SPKTLLTFCTNGVLLRTLMAGDSTLSTVTHVIVDEVHERDRFSDFLLTKLRDLLQKHPT
LKLILSSAALDVNLFIRYFGSCPVIYIQGRPFEVKEMFLEDILRTTGYTNKEMLKYKKE
KQQEEKQQTTLTEWYSAQENSFKPESQRQRTVLNVTDEYDLLDDGGDAVFSQLTEKDVN
CLEPWLIKEMDACLSDIWLHKDIDAFAQVFHLILTENVSVDYRHSETSATALMVAAGRG
FASQVEQLISMGANVHSKASNGWMALDWAKHFGQTEIVDLLESYSATLEFGNLDESSLV
QTNGSDLSAEDRELLEAYHHSFDDEKVDLDLIMHLLYNICHSCDAGAVLIFLPGYDEIV
GLRDRILFDDKRFADSTHRYQVFMLHSNMQTSDQKKVLKNPPAGVRKIILSTNIAETSI
TVNDVVFVIDSGKVKEKSFDALNFVTMLKMVWISKASAIQRKGRA"
misc_feature 861..866
/label=SDS
intron 2955..2995
/label=Splicing Donor
misc_feature 2999..3032
/label=lox2272-JT1571
repeat_region 3077..3217
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3077..3206
/label=AAV2 ITR
rep_origin 3292..3747
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4029..4133
/gene="bla"
/label=AmpR promoter
CDS 4134..4994
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5165..5753
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtggatct gcgatcgctc cggtgcccgt cagtgggcag
181 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg
241 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
301 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
361 gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac
421 gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc
481 ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga
541 gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc
601 tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt
661 acagatccaa gctgtgaccg gcgcctaccg tacggccacc atgtaccctt atgacgtacc
721 tgactatgct ggatcttcca ggccgagcag cgtctccccg cggcagccgg ctcctggcgg
781 tggcggaggc ggcggcccct cgccttgtgg ccctgggggc ggcggccggg ccaaggggct
841 gaaggacatt cgcattgatg aggaggtgaa gatcgcagtc aatatcgcgc tggagcgctt
901 ccgatacggg gaccagagag aaatggaatt tccttcttct ttgaccagta ctgaaagagc
961 ctttattcat cgactcagtc agtctcttgg tttggtctct aaaagtaaag gaaagggagc
1021 aaatagatac ctaactgtga agaagaaaga tggatcagaa acagctcatg caatgatgac
1081 ctgtaatttg actcataata caaaacatgc tgttaggagc ctaattcaaa gatttcctgt
1141 caccaataaa gagcgtacag aacttctgcc taaaacagaa agaggaaatg tgtttgcagt
1201 tgaagctgaa aaccgggaaa tgagcaagac aagtgggcga ctcaacaatg gcatacctca
1261 gattccagtg aaaagaggag aatccgaatt tgattctttt aggcagtctt taccagtgtt
1321 tgagaaacag gaagaaattg ttaaaataat taaggaaaat aaagtagttt tgattgtagg
1381 agaaactggg tctggaaaga ccacacagat tcctcagttc cttttagatg attgctttaa
1441 aaatggtatc ccctgccgta tattttgtac tcaaccaaga cgattggcag ctatcgctgt
1501 ggctgaaaga gttgccgcag agagacggga aaggattggt caaacaattg gttatcagat
1561 ccgattagaa agcagggttt ctccaaagac acttctgaca ttttgtacta atggggtatt
1621 gcttcgtaca ttgatggcag gagatagtac gttgtcgact gtgacacatg ttatcgtgga
1681 tgaagtgcat gaaagggatc gatttagtga ttttttactt acaaagttaa gagatttgtt
1741 gcaaaagcac ccaactttga aactaattct ttctagtgct gccttggatg taaatctctt
1801 tataagatat tttggaagtt gtccagtgat atatatacag ggaagaccat ttgaagtaaa
1861 agaaatgttt ctggaagata ttttaagaac aactggatat acaaacaaag aaatgttaaa
1921 atataaaaag gaaaaacagc aagaagagaa acaacaaacc acacttacag aatggtactc
1981 agctcaagaa aatagtttca agcctgaatc tcagaggcag agaactgttc taaatgtgac
2041 tgatgagtat gacttactgg atgatggtgg tgatgctgtc ttcagtcagc tgactgaaaa
2101 agatgtgaat tgccttgaac catggttaat aaaggaaatg gatgcttgcc tttctgatat
2161 atggctacat aaagatattg atgcctttgc tcaggtcttt catctcattt taactgaaaa
2221 tgttagtgtt gattacagac atagtgaaac cagtgcaaca gctctgatgg ttgctgcagg
2281 acgtggcttt gcaagtcaag tagaacagtt aatcagtatg ggagccaatg tccatagtaa
2341 agcatcaaat ggctggatgg cattggattg ggctaaacac tttgggcaga ctgaaattgt
2401 ggatcttcta gaatcttaca gtgctacact ggaatttgga aatctagatg aaagttctct
2461 ggttcaaaca aatggaagtg acctcagtgc tgaagacaga gagctcctgg aagcttatca
2521 tcatagtttc gatgatgaaa aagtagactt ggatttgatc atgcatcttc tatacaatat
2581 ctgccatagt tgtgatgctg gtgcagtact aatttttctg cctggatatg acgaaattgt
2641 tggactgaga gatcgcatcc tgtttgatga caagcggttt gctgacagta cacatagata
2701 ccaagtcttt atgcttcatt caaatatgca aacatccgat caaaagaaag tattaaaaaa
2761 cccacctgca ggtgttcgaa aaataattct ttccaccaat attgctgaaa ccagcatcac
2821 agtcaatgat gttgtctttg ttattgattc tggtaaggtg aaagagaaat cctttgatgc
2881 tctgaatttt gttacaatgt taaaaatggt atggatttcc aaagctagtg ccatacagcg
2941 gaaaggcagg gcaggtaagt atcaaggtta caagacaggt ttaaggagac caataagcta
3001 ttattcgtat aggatacttt atacgaagtt attaagttat ggagaccggt gtggcacgtg
3061 cggaccgagc ggccgcagga acccctagtg atggagttgg ccactccctc tctgcgcgct
3121 cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg
3181 gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc
3241 cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct
3301 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
3361 ccagcgcctt agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
3421 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
3481 ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct
3541 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
3601 tccaaactgg aacaacactc aactctatct cgggctattc ttttgattta taagggattt
3661 tgccgatttc ggtctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
3721 ttaacaaaat attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg
3781 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
3841 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
3901 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
3961 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
4021 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
4081 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
4141 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
4201 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
4261 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
4321 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
4381 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
4441 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
4501 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
4561 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
4621 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
4681 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
4741 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
4801 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
4861 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
4921 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
4981 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
5041 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
5101 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
5161 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
5221 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
5281 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
5341 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
5401 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
5461 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
5521 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
5581 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
5641 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
5701 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
5761 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgt
//
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