| 质粒名称: | pAAVLINK-EF1a-HA-5'-CLTC |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001599 |
| 产品用途: | 5' AAVLINK plasmid for CLTC expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | CLTC |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EF1a-HA-5'-CLTC
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EF1a-HA-5'-CLTC载体质粒基本信息
pAAVLINK-EF1a-HA-5'-CLTC质粒图谱载体图谱和pAAVLINK-EF1a-HA-5'-CLTC载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-HA-5'-CLTC质粒载体简介
pAAVLINK-EF1a-HA-5'-CLTC质粒序列载体序列
LOCUS pAAVLINK-EF1a-HA-5'-CLTC 6527 bp DNA circular SYN 18-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6527)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 20, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6527
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 187..398
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 411..679
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 505..510
/label=SDS
CDS 716..742
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 749..3667
/codon_start=1
/label=CLTC
/translation="MAQILPIRFQEHLQLQNLGINPANIGFSTLTMESDKFICIREKVG
EQAQVVIIDMNDPSNPIRRPISADSAIMNPASKVIALKAGKTLQIFNIEMKSKMKAHTM
TDDVTFWKWISLNTVALVTDNAVYHWSMEGESQPVKMFDRHSSLAGCQIINYRTDAKQK
WLLLTGISAQQNRVVGAMQLYSVDRKVSQPIEGHAASFAQFKMEGNAEESTLFCFAVRG
QAGGKLHIIEVGTPPTGNQPFPKKAVDVFFPPEAQNDFPVAMQISEKHDVVFLITKYGY
IHLYDLETGTCIYMNRISGETIFVTAPHEATAGIIGVNRKGQVLSVCVEEENIIPYITN
VLQNPDLALRMAVRNNLAGAEELFARKFNALFAQGNYSEAAKVAANAPKGILRTPDTIR
RFQSVPAQPGQTSPLLQYFGILLDQGQLNKYESLELCRPVLQQGRKQLLEKWLKEDKLE
CSEELGDLVKSVDPTLALSVYLRANVPNKVIQCFAETGQVQKIVLYAKKVGYTPDWIFL
LRNVMRISPDQGQQFAQMLVQDEEPLADITQIVDVFMEYNLIQQCTAFLLDALKNNRPS
EGPLQTRLLEMNLMHAPQVADAILGNQMFTHYDRAHIAQLCEKAGLLQRALEHFTDLYD
IKRAVVHTHLLNPEWLVNYFGSLSVEDSLECLRAMLSANIRQNLQICVQVASKYHEQLS
TQSLIELFESFKSFEGLFYFLGSIVNFSQDPDVHFKYIQAACKTGQIKEVERICRESNC
YDPERVKNFLKEAKLTDQLPLIIVCDRFDFVHDLVLYLYRNNLQKYIEIYVQKVNPSRL
PVVIGGLLDVDCSEDVIKNLILVVRGQFSTDELVAEVEKRNRLKLLLPWLEARIHEGCE
EPATHNALAKIYIDSNNNPERFLRENPYYDSRVVGKYCEKRDPHLACVAYERGQCDLEL
INVCNENSLFKSLSRYLVRRKDPELWGSVLLESNPYRRPLIDQ"
intron 3668..3708
/label=Splicing Donor
misc_feature 3712..3745
/label=lox2272-JT1571
repeat_region 3790..3930
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3790..3919
/label=AAV2 ITR
rep_origin 4005..4460
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4742..4846
/gene="bla"
/label=AmpR promoter
CDS 4847..5707
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5878..6466
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgtaccc
721 ttatgacgta cctgactatg ctggatctat ggcccagatt ctgccaattc gttttcagga
781 gcatctccag ctccagaacc tgggtatcaa cccagcaaac attggcttca gtaccctgac
841 tatggagtct gacaaattca tctgcattag agaaaaagta ggagagcagg cccaggtggt
901 aatcattgat atgaatgacc caagtaatcc aattcgaaga ccaatttcag cagacagcgc
961 catcatgaat ccagctagca aagtaattgc actgaaagct gggaaaactc ttcagatttt
1021 taacattgaa atgaaaagta aaatgaaggc tcataccatg actgatgatg tcaccttttg
1081 gaaatggatc tctttgaata cggttgctct tgttacggat aatgcagttt atcactggag
1141 tatggaagga gagtctcagc cagtgaaaat gtttgatcgc cattctagcc ttgcagggtg
1201 ccagattatc aattaccgta cagatgcaaa acaaaagtgg ttacttctga ctggtatatc
1261 tgcacagcaa aatcgtgtgg tgggagctat gcagctatat tctgtagata ggaaagtgtc
1321 tcagcccatt gaaggacatg cagctagctt tgcacagttt aagatggaag gaaatgcaga
1381 agaatcaacg ttattttgtt ttgcagttcg gggccaagct ggagggaagt tacatattat
1441 tgaagttggc acaccaccta cagggaacca gccctttcca aagaaggcag tggatgtctt
1501 ctttcctcca gaagcacaaa atgattttcc tgttgcaatg cagatcagtg aaaagcatga
1561 tgtggtgttc ttgataacca agtatggtta tatccacctc tatgatcttg agactggtac
1621 ctgcatctac atgaatagaa tcagtggaga aacaattttt gttactgcac ctcatgaagc
1681 cacagctgga ataattggag taaacagaaa gggacaagtt ctgtcagtgt gtgtggaaga
1741 agaaaacata attccttaca tcaccaatgt tctacaaaat cctgatttgg ctctgagaat
1801 ggctgtacgt aataacttag ccggtgctga agaactcttt gcccggaaat ttaatgctct
1861 ttttgcccag ggaaattact cggaggcagc aaaggtggct gctaatgcac caaagggaat
1921 tcttcgtact ccagacacta tccgtcggtt ccagagtgtc ccagcccagc caggtcaaac
1981 ttctcctcta cttcagtact ttggtatcct tttggaccag ggacagctca acaaatacga
2041 atccttagag ctttgtaggc ctgtacttca gcaagggcga aaacagcttt tggagaaatg
2101 gttaaaagaa gataagctgg aatgttctga agaactgggt gatcttgtga aatctgtgga
2161 ccctacattg gcacttagtg tgtacctaag ggctaacgtc ccaaataaag tcattcagtg
2221 ctttgcagaa acaggtcaag tccaaaagat tgttttatat gctaaaaaag ttggatacac
2281 tccagattgg atatttctgc tgagaaatgt aatgcgaatc agtccagatc agggacagca
2341 gtttgcccaa atgttagttc aagatgaaga gcctcttgct gacatcacac agattgtaga
2401 tgtctttatg gaatacaatc taattcagca gtgtactgca ttcttgcttg atgctctgaa
2461 gaataatcgc ccatctgaag gtcctttaca gacgcggtta cttgagatga accttatgca
2521 tgcgcctcaa gttgcagatg ctattctagg caatcagatg ttcacacatt atgaccgggc
2581 tcatattgct caactgtgtg aaaaggctgg cctactgcag cgtgcattag aacatttcac
2641 tgatttatat gatataaaac gtgcagtggt tcacacccat cttcttaacc ctgagtggtt
2701 agtcaactac tttggttcct tatcagtaga agactcccta gaatgtctca gagccatgct
2761 gtctgccaac atccgtcaga atctgcagat ttgtgttcag gtggcttcta aatatcatga
2821 acaactgtca actcagtctc tgattgaact ttttgaatct ttcaagagtt ttgaaggtct
2881 cttttatttt ctgggatcca ttgttaactt tagccaggac ccagatgtgc actttaaata
2941 tattcaggca gcttgcaaga ctgggcaaat caaagaagta gaaagaatct gtagagaaag
3001 caactgctac gatcctgagc gagtcaagaa ttttcttaag gaagcaaaac taacagatca
3061 gctaccactt atcattgtgt gtgatcgatt tgactttgtc catgatttgg tgctctattt
3121 atatagaaat aatcttcaaa agtatataga gatatatgta cagaaggtga atccaagtcg
3181 acttcctgta gttattggag gattacttga tgttgactgt tctgaagatg tcataaaaaa
3241 cttgattctt gttgtaagag gtcaattctc tactgatgag cttgttgctg aggttgaaaa
3301 aagaaacaga ttgaaactgc ttctgccttg gctagaggcc agaattcatg agggctgtga
3361 ggagcctgct actcacaatg ccttagccaa aatctacata gacagtaata acaacccgga
3421 gagatttctt cgtgaaaatc cctactatga cagtcgcgtt gttggaaagt attgtgagaa
3481 gagagatcca catctggcct gtgttgctta tgaacgtggc caatgtgatc tggaacttat
3541 taatgtttgc aatgagaatt ccctcttcaa aagtctttct cgctacctgg tacgtcgaaa
3601 ggatccagaa ttgtggggca gcgtgctgct ggaaagcaat ccttacagga gacccctaat
3661 tgaccaggta agtatcaagg ttacaagaca ggtttaagga gaccaataag ctattattcg
3721 tataggatac tttatacgaa gttattaagt tatggagacc ggtgtggcac gtgcggaccg
3781 agcggccgca ggaaccccta gtgatggagt tggccactcc ctctctgcgc gctcgctcgc
3841 tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag
3901 tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc
3961 atctgtgcgg tatttcacac cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg
4021 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
4081 cttagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
4141 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
4201 cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac
4261 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
4321 tggaacaaca ctcaactcta tctcgggcta ttcttttgat ttataaggga ttttgccgat
4381 ttcggtctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
4441 aatattaacg tttacaattt tatggtgcac tctcagtaca atctgctctg atgccgcata
4501 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
4561 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
4621 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata
4681 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
4741 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
4801 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
4861 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
4921 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
4981 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
5041 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
5101 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
5161 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
5221 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
5281 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
5341 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
5401 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
5461 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
5521 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
5581 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
5641 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
5701 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
5761 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
5821 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
5881 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
5941 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
6001 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
6061 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
6121 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
6181 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
6241 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
6301 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
6361 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
6421 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
6481 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgt
//
