| 质粒名称: | pAAVLINK-EF1a-5'-PCDH15 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001581 |
| 产品用途: | 5' AAVLINK plasmid for PCDH15 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: |
EF1a
|
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | PCDH15 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EF1a-5'-PCDH15
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EF1a-5'-PCDH15载体质粒基本信息
pAAVLINK-EF1a-5'-PCDH15质粒图谱载体图谱和pAAVLINK-EF1a-5'-PCDH15载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-5'-PCDH15质粒载体简介
pAAVLINK-EF1a-5'-PCDH15质粒序列载体序列
LOCUS pAAVLINK-EF1a-5'-PCDH15 6831 bp DNA circular SYN 18-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6831)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 19, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6831
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 158..700
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 187..398
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 411..679
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 505..510
/label=SDS
CDS 713..3971
/codon_start=1
/label=PCDH15
/translation="MFRQFYLWTCLASGIILGSLFEICLGQYDDDCKLARGGPPATIVA
IDEESRNGTILVDNMLIKGTAGGPDPTIELSLKDNVDYWVLMDPVKQMLFLNSTGRVLD
RDPPMNIHSIVVQVQCINKKVGTIIYHEVRIVVRDRNDNSPTFKHESYYATVNELTPVG
TTIFTGFSGDNGATDIDDGPNGQIEYVIQYNPDDPTSNDTFEIPLMLTGNIVLRKRLNY
EDKTRYFVIIQANDRAQNLNERRTTTTTLTVDVLDGDDLGPMFLPCVLVPNTRDCRPLT
YQAAIPELRTPEELNPIIVTPPIQAIDQDRNIQPPSDRPGILYSILVGTPEDYPRFFHM
HPRTAELSLLEPVNRDFHQKFDLVIKAEQDNGHPLPAFAGLHIEILDENNQSPYFTMPS
YQGYILESAPVGATISDSLNLTSPLRIVALDKDIEDTKDPELHLFLNDYTSVFTVTQTG
ITRYLTLLQPVDREEQQTYTFSITAFDGVQESEPVIVNIQVMDANDNTPTFPEISYDVY
VYTDMRPGDSVIQLTAVDADEGSNGEITYEILVGAQGDFIINKTTGLITIAPGVEMIVG
RTYALTVQAADNAPPAERRNSICTVYIEVLPPNNQSPPRFPQLMYSLEISEAMRVGAVL
LNLQATDREGDSITYAIENGDPQRVFNLSETTGILTLGKALDRESTDRYILIITASDGR
PDGTSTATVNIVVTDVNDNAPVFDPYLPRNLSVVEEEANAFVGQVKATDPDAGINGQVH
YSLGNFNNLFRITSNGSIYTAVKLNREVRDYYELVVVATDGAVHPRHSTLTLAIKVLDI
DDNSPVFTNSTYTVLVEENLPAGTTILQIEAKDVDLGANVSYRIRSPEVKHFFALHPFT
GELSLLRSLDYEAFPDQEASITFLVEAFDIYGTMPPGIATVTVIVKDMNDYPPVFSKRI
YKGMVAPDAVKGTPITTVYAEDADPPGLPASRVRYRVDDVQFPYPASIFEVEEDSGRVI
TRVNLNEEPTTIFKLVVVAFDDGEPVMSSSATVKILVLHPGEIPRFTQEEYRPPPVSEL
ATKGTMVGVISAAAINQSIVYSIVSGNEEDTFGINNIT"
misc_feature 2480..2485
/label=SDS
misc_feature 2549..2554
/label=SDS
misc_feature 3479..3484
/label=SDS
misc_feature 3575..3580
/label=SDS
misc_feature 3822..3827
/label=SDS
misc_feature 3836..3841
/label=SDS
intron 3972..4012
/label=Splicing Donor
misc_feature 4016..4049
/label=lox2272-JT1571
repeat_region 4094..4234
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4094..4223
/label=AAV2 ITR
rep_origin 4309..4764
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5046..5150
/gene="bla"
/label=AmpR promoter
CDS 5151..6011
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6182..6770
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgtttcg
721 acagttttat ctctggacat gtttagcttc agggatcatc ctgggctctc tctttgaaat
781 ctgcttgggc cagtatgatg atgattgcaa actagctagg ggaggaccac cagctaccat
841 agttgctatt gatgaagaaa gtcggaatgg tacaattctg gtggacaaca tgctgatcaa
901 agggactgct ggaggaccag accccaccat agaactttct ttaaaggata atgtggatta
961 ctgggtgttg atggatcctg ttaagcaaat gcttttcctg aacagcaccg gaagagttct
1021 ggatagagat ccaccgatga acatacactc cattgtggtg caggtccagt gcatcaacaa
1081 aaaagtgggc actattatct accatgaagt gcgaatagtg gtgagagaca ggaatgacaa
1141 ctcacccact ttcaagcatg aaagctacta tgccacagtg aatgagctca ctccagttgg
1201 taccacaata ttcacaggat tttcaggaga caatggagct acagatatag atgatggacc
1261 aaatggacag atagagtatg ttattcagta taatccagat gatccgacat ccaatgacac
1321 ctttgaaatt cccctaatgt tgactggaaa tatagtgtta aggaagaggc tcaactatga
1381 agataagact cgctactttg tcataatcca agctaatgac cgtgcccaaa atctgaatga
1441 gaggcgaacc accaccacca ctctcacagt ggatgttctg gatggagatg acttgggtcc
1501 aatgtttctt ccttgtgtcc ttgtgccaaa cactcgtgat tgccgtccac tcacttatca
1561 agctgccata cctgagttga gaactccgga agaactgaac cccattattg ttacgccacc
1621 aatccaagcc attgatcagg accggaatat tcaaccgcca tcagataggc caggaatcct
1681 ctattccatc cttgttggga ctcctgagga ttacccacga tttttccata tgcatcctag
1741 gacagcagaa cttagtctcc tggagccagt aaacagagac tttcaccaga aatttgattt
1801 ggttattaag gctgaacaag acaatggtca tcctcttcct gcctttgccg gtctacacat
1861 tgaaatactg gatgaaaaca atcaaagtcc atattttaca atgcccagtt atcaaggcta
1921 tatcctggaa tctgccccag tgggagcaac catttcggac agtctcaatt tgacttcacc
1981 tttaagaata gtagctctgg acaaggacat agaagataca aaagacccag agcttcacct
2041 ttttctgaat gactacacct cagtcttcac cgtcacacag actggtatta ctcgctacct
2101 caccttactt caaccagtgg acagggaaga acagcaaact tacacctttt cgataacagc
2161 atttgatggt gtacaagaaa gtgagccagt catcgtcaat attcaagtga tggatgcaaa
2221 tgataacacg ccaaccttcc ctgaaatatc ctatgatgtg tatgtttata cagacatgag
2281 acctggggac agtgtcatac agctcactgc agtcgacgca gacgaagggt caaatgggga
2341 gatcacatat gaaatccttg ttggggctca gggagacttc atcatcaata aaacaacagg
2401 gcttatcacc atcgctccag gggtggaaat gatagtcggg cggacttacg cactcacggt
2461 ccaagcagcg gataatgctc ctcctgcaga gcgaaggaac tccatctgca ctgtgtatat
2521 tgaagtgctt ccaccaaata atcaaagccc tcctcgcttc ccacagctga tgtatagcct
2581 tgaaattagt gaagccatga gggttggtgc tgttttatta aatctacagg caactgatcg
2641 agagggagac tcaataacat atgccattga gaatggagat cctcagagag tttttaatct
2701 ttcagaaacc acggggattc taaccttagg gaaagcactg gacagggaaa gcactgatcg
2761 ctacattctg atcatcacag cttcagatgg caggccagat gggacctcaa ctgccacagt
2821 aaacatagtg gtgacagatg tcaatgacaa tgctccagtg tttgatcctt atctgccaag
2881 aaatttatct gtggtggaag aagaagccaa tgcctttgtg ggtcaagtaa aagcaacaga
2941 ccctgatgct ggaataaatg gtcaagtgca ctacagtttg ggtaacttta ataatctttt
3001 tcgtatcaca tccaatggga gcatttacac agcagtgaag cttaacagag aagtcaggga
3061 ctactatgaa cttgttgttg tggcaacaga tggagcagta caccctcgtc attcaactct
3121 aaccttggcc atcaaggttt tggacattga tgataacagt cctgtgttca ccaattcaac
3181 atacactgtc cttgttgaag agaatttgcc agctgggact accatccttc aaatagaggc
3241 caaagatgtc gaccttggag caaatgtgtc ttaccggata agaagcccag aagtgaagca
3301 cttttttgca ctacatccat ttacaggaga actatcgctt ttaaggagtt tagattatga
3361 ggcatttcca gaccaagaag caagtatcac ttttctggta gaggcctttg atatttatgg
3421 aacaatgcca cctggtattg ctactgtcac agtgattgta aaggatatga atgattatcc
3481 tcctgtcttt agtaaacgaa tatacaaagg gatggtggct ccggatgcag tcaagggtac
3541 acctatcaca acagtttatg ctgaagatgc agaccctcct ggattacctg caagtcgtgt
3601 gaggtataga gtagatgatg tacagtttcc ttaccctgcc agtatttttg aagtggaaga
3661 agattctgga agagtaataa cacgagtcaa tcttaatgaa gaacctacaa caatttttaa
3721 gttggtggtg gttgcttttg atgatgggga gcctgtgatg tccagcagtg ccacagtgaa
3781 gattcttgtc ttacatcctg gtgagatccc acgcttcaca caggaggaat atagacctcc
3841 tccagtaagt gaacttgcca ccaaagggac catggttggt gtaatttctg ctgctgccat
3901 taatcaaagt attgtgtact ccattgtttc aggaaatgaa gaagatacat ttggaattaa
3961 taacatcaca ggtaagtatc aaggttacaa gacaggttta aggagaccaa taagctatta
4021 ttcgtatagg atactttata cgaagttatt aagttatgga gaccggtgtg gcacgtgcgg
4081 accgagcggc cgcaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc
4141 tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc
4201 tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc tgatgcggta ttttctcctt
4261 acgcatctgt gcggtatttc acaccgcata cgtcaaagca accatagtac gcgccctgta
4321 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
4381 gcgccttagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
4441 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
4501 acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat
4561 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
4621 aaactggaac aacactcaac tctatctcgg gctattcttt tgatttataa gggattttgc
4681 cgatttcggt ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
4741 acaaaatatt aacgtttaca attttatggt gcactctcag tacaatctgc tctgatgccg
4801 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
4861 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
4921 ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt
4981 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
5041 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
5101 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
5161 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
5221 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
5281 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
5341 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
5401 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
5461 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
5521 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
5581 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
5641 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
5701 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
5761 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
5821 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
5881 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
5941 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
6001 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
6061 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
6121 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
6181 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
6241 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
6301 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
6361 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
6421 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
6481 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
6541 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
6601 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
6661 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
6721 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
6781 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg t
//
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pAAVLINK-EF1a-5'-PCDH15质粒载体应用举例
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