| 质粒名称: | pLZ416 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000631 |
| 产品用途: | Mammalian Cre-repressible GEMSANR expression vector (PhCMV-loxP-GEMSANR-loxP-pA). |
| 存储实验室: | Mingqi XIE Lab |
|
载体骨架基本信息 |
|
| 载体名称: |
pCMV
|
| 表达系统: | 哺乳动物表达 |
| 启动子: | PhCMV |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | GEMSANR |
| 物种来源: | synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Multi-layered computational gene networks by engineered tristate logics
Shao J., Qiu X., Zhang L., Li S., Xue S., Si Y., Li Y., Jiang J., Wu Y., Xiong Q., Wang Y., Chen Q., Gao T., Zhu L., Wang H., Xie M.
Cell 187, 5064-5080.e14 (2024)
|
pLZ416
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pLZ416载体质粒基本信息
pLZ416质粒图谱载体图谱和pLZ416载体序列质粒序列多克隆位点信息
pLZ416质粒载体简介
pLZ416质粒序列载体序列
LOCUS pLZ416 9414 bp DNA circular SYN 10-DEC-2024
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9414)
AUTHORS Trial User
TITLE Direct Submission
JOURNAL Exported Jul 20, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..9414
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 235..614
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 643..668
/label=185-WKG-602-CMV-F
primer_bind 769..789
/label=oMHS-CMV-F
misc_feature 829
/label=putative transcriptional start
promoter 863..882
/label=T7 promoter
promoter 863..881
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
misc_recomb 905..938
/label=LoxP
protein_bind complement(905..938)
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (ATGTATGC) (Shaw et al., 2021)."
regulatory 1001..1010
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
misc_feature 1007..1069
/label=Igk secretion signal
regulatory 1082..1091
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 1090
/codon_start=1
/label=5xGS Linker
/translation=""
CDS 1091..1153
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 1154..1228
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
CDS 1229..1291
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 1292..1366
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
CDS 1373..1435
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 1436..1510
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
CDS 1511..1573
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 1574..1648
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
CDS 1655..1717
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 1718..1792
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
CDS 1793..1855
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 1856..1930
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
CDS 1937..1999
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 2000..2074
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
CDS 2075..2137
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 2138..2212
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
misc_feature 2225..2968
/label=mouse EpoR-D1_D2_TM
/note="mouse EpoR-D1_D2_TM"
primer_bind complement(2292..2314)
/label=186-WKG-603-R
misc_feature 2498..2500
/label=F93A
/note="F93A"
misc_feature 2952..2968
/label=EpoR-D1_D2_TM
misc_feature 2978..3806
/label=IL6ST
misc_feature 3005..3031
/label=box1
misc_feature 3329..3331
/label=Y759A
primer_bind 3701..3729
/label=200-WKG-631-F1
polyA_signal 3835..4059
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
terminator 4068..4252
/label=SV40 late polyA
protein_bind complement(4289..4322)
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (ATGTATGC) (Shaw et al., 2021)."
misc_feature 4335..4403
/label=Secretory (IgK)
/label=nonstandard type: signal
CDS 4422..4934
/codon_start=1
/product="NanoLuc(R) luciferase"
/label=Nluc
/note="human codon-optimized"
/translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
RLCERILA"
misc_feature 4455..4457
/label=cyt-STOP (TGG->TGA)
primer_bind 4730..4759
/label=201-WKG-631-F2
terminator 4941..4963
/label=SV40 late polyA
polyA_signal 5011..5235
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 5281..5709
/label=f1 bacteriophage origin of replication
/label=f1 bacteriophage origin of replication; arrow _f1
ori
primer_bind complement(5295..5319)
/label=184-WKG-599-R
primer_bind 5580..5599
/label=189-WKG-612-F
promoter 5723..6052
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
misc_feature 5955..6004
/label=P_SV40 TSS-region
CDS 6119..6910
/label=Kan/neoR
/label=nonstandard type: resistance
primer_bind complement(6163..6190)
/label=188-WKG-610-R
polyA_signal 7087..7208
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(7253..7273)
/label=M13-rev
primer_bind complement(7257..7273)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
misc_binding complement(7279..7301)
/label=LacO
protein_bind 7281..7297
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(7305..7335)
/label=lac promoter
primer_bind complement(7318..7341)
/label=190-WKG-612-R
protein_bind 7350..7371
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
misc_feature 7477..8417
/label=ColE1
/note="ColE1"
misc_feature complement(8421..9278)
/label=AmpR
/label=nonstandard type: resistance
promoter complement(9279..9383)
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gcttataact tcgtatagca tacattatac gaagttatcg gcgcgccgcg gccgcgatca
961 attcggtacc gaagttccta ttccgaattc ctagtggccg gccaccatgg agaccgatac
1021 cttattactg tgggtgcttc ttctgtgggt tcccggtagc accggagacg gatccaagct
1081 tgccaccatg ggggagctcg acgagttggt ttatctctta gacggacccg gatatgaccc
1141 cattcattca gatggctccg gaacgggcag cgggaccgga agtggcaccg gaaccacttc
1201 cggaaccggt actggcggga gtaccggcgg tgagctagac gaactcgtgt acctgttgga
1261 tggccccggt tacgacccaa tccacagcga tggaagtgga actgggtctg ggactgggtc
1321 cggcaccggg actacttcag gcaccggcac tgggggctcc actggccata ccggggagct
1381 cgacgagttg gtttatctct tagacggacc cggatatgac cccattcatt cagatggctc
1441 cggaacgggc agcgggaccg gaagtggcac cggaaccact tccggaaccg gtactggcgg
1501 gagtaccggc ggtgagctag acgaactcgt gtacctgttg gatggccccg gttacgaccc
1561 aatccacagc gatggaagtg gaactgggtc tgggactggg tccggcaccg ggactacttc
1621 aggcaccggc actgggggct ccactggcca taccggggag ctcgacgagt tggtttatct
1681 cttagacgga cccggatatg accccattca ttcagatggc tccggaacgg gcagcgggac
1741 cggaagtggc accggaacca cttccggaac cggtactggc gggagtaccg gcggtgagct
1801 agacgaactc gtgtacctgt tggatggccc cggttacgac ccaatccaca gcgatggaag
1861 tggaactggg tctgggactg ggtccggcac cgggactact tcaggcaccg gcactggggg
1921 ctccactggc cataccgggg agctcgacga gttggtttat ctcttagacg gacccggata
1981 tgaccccatt cattcagatg gctccggaac gggcagcggg accggaagtg gcaccggaac
2041 cacttccgga accggtactg gcgggagtac cggcggtgag ctagacgaac tcgtgtacct
2101 gttggatggc cccggttacg acccaatcca cagcgatgga agtggaactg ggtctgggac
2161 tgggtccggc accgggacta cttcaggcac cggcactggg ggctccactg gccatatgga
2221 attcgcacct tcacccagcc tcccggaccc caagtttgag agcaaagcgg ccctgctggc
2281 atcccggggc tccgaagaac ttctgtgctt cacccaacgc ttggaagact tggtgtgttt
2341 ctgggaggaa gcggcgagct ccgggatgga cttcaactac agcttctcat accagctcga
2401 gggtgagtca cgaaagtcat gtagcctgca ccaggctccc accgtccgcg gctccgtgcg
2461 tttctggtgt tcactgccaa cagcggacac atcgagtgct gtgccgctgg agctgcaggt
2521 gacggaggcg tccggttctc ctcgctatca ccgcatcatc catatcaatg aagtagtgct
2581 cctggacgcc cccgcggggc tgctggcgcg ccgggcagaa gagggcagcc acgtggtgct
2641 gcgctggctg ccacctcctg gagcacctat gaccacccac atccgatatg aagtggacgt
2701 gtcggcaggc aaccgggcag gagggacaca aagggtggag gtcctggaag gccgcactga
2761 gtgtgttctg agcaacctgc ggggcgggac gcgctacacc ttcgctgttc gagcgcgcat
2821 ggccgagccg agcttcagcg gattctggag tgcctggtct gagcccgcgt cactactgac
2881 cgctagcgac ctggaccctc tcatcttgac gctgtctctc attctggtcc tcatctcgtt
2941 gttgctgacg gttctggccc tgctgtccgc agcagcaaat aagcgagacc taattaaaaa
3001 acacatctgg cctaatgttc cagatccttc aaagagtcat attgcccagt ggtcacctca
3061 cactcctcca aggcacaatt ttaattcaaa agatcaaatg tattcagatg gcaatttcac
3121 tgatgtaagt gttgtggaaa tagaagcaaa tgacaaaaag ccttttccag aagatctgaa
3181 atcattggac ctgttcaaaa aggaaaaaat taatactgaa ggacacagca gtggtattgg
3241 ggggtcttca tgcatgtcat cttctaggcc aagcatttct agcagtgatg aaaatgaatc
3301 ttcacaaaac acttcgagca ctgtccaggc ttctaccgtg gtacacagtg gctacagaca
3361 ccaagttccg tcagtccaag tcttctcaag atccgagtct acccagccct tgttagattc
3421 agaggagcgg ccagaagatc tacaattagt agatcatgta gatggcggtg atggtatttt
3481 tcccaggcaa cagtacttca aacagaactg cagtcagcat gaatccagtc cagatatttc
3541 acattttgaa aggtcaaagc aagtttcatc agtcaatgag gaagattttg ttagacttaa
3601 acagcagatt tcagatcata tttcacaatc ctgtggatct gggcaaatga aaatgtttca
3661 ggaagtttct gcagcagatg cttttggtcc aggtactgag ggacaagtag aaagatttga
3721 aacagttggc atggaggctg cgactgatga aggcatgcct aaaagttact taccacagac
3781 tgtacggcaa ggcggctaca tgcctcagta acctgcaggg ctgatcagcc tcgactgtgc
3841 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
3901 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
3961 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
4021 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggat aagatacatt
4081 gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt
4141 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac
4201 aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcgct
4261 tggtaccgtc tttccagtgg ttaattaaat aacttcgtat agcatacatt atacgaagtt
4321 atctcgaggc caccatgact agtgagacag acacactcct gctatgggta ctgctgctct
4381 gggttccagg ttccactggt gacgctagtg gtggttctgg tatggtcttc acactcgaag
4441 atttcgttgg ggactggcga cagacagccg gctacaacct ggaccaagtc cttgaacagg
4501 gaggtgtgtc cagtttgttt cagaatctcg gggtgtccgt aactccgatc caaaggattg
4561 tcctgagcgg tgaaaatggg ctgaagatcg acatccatgt catcatcccg tatgaaggtc
4621 tgagcggcga ccaaatgggc cagatcgaaa aaatttttaa ggtggtgtac cctgtggatg
4681 atcatcactt taaggtgatc ctgcactatg gcacactggt aatcgacggg gttacgccga
4741 acatgatcga ctatttcgga cggccgtatg aaggcatcgc cgtgttcgac ggcaaaaaga
4801 tcactgtaac agggaccctg tggaacggca acaaaattat cgacgagcgc ctgatcaacc
4861 ccgacggctc cctgctgttc cgagtaacca tcaacggagt gaccggctgg cggctgtgcg
4921 aacgcattct ggcgatcgat agacatgata agatacattg atgtctagaa gcttctagag
4981 ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
5041 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
5101 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
5161 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
5221 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc
5281 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
5341 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
5401 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
5461 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
5521 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
5581 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
5641 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
5701 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
5761 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
5821 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
5881 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
5941 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga
6001 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
6061 gggagcttgt atatccattt tcggatctga tcaagagaca ggatgaggat cgtttcgcat
6121 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
6181 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
6241 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
6301 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
6361 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
6421 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
6481 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
6541 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
6601 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg
6661 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
6721 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
6781 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
6841 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
6901 cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg
6961 ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
7021 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
7081 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
7141 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
7201 gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca
7261 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
7321 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
7381 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
7441 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
7501 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
7561 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
7621 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
7681 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
7741 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
7801 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
7861 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
7921 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
7981 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
8041 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
8101 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
8161 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
8221 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
8281 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
8341 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
8401 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
8461 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
8521 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
8581 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
8641 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
8701 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
8761 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
8821 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
8881 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
8941 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
9001 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
9061 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
9121 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
9181 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
9241 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
9301 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
9361 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtc
//
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