pLZ538

LOCUS       pLZ538           4593 bp DNA     circular SYN 02-APR-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4593)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Jul 20, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..4593
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    309..443
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(325..344)
                     /label=PCMV-R
     primer_bind     complement(333..352)
                     /label=EBV-R
     primer_bind     complement(341..360)
                     /label=PCMV-myc-R-bd
     primer_bind     complement(343..362)
                     /label=pCI-neo-R
     primer_bind     346..366
                     /label=PSG5-R(SV40-R)
     primer_bind     410..431
                     /label=PFastBac1-R
     primer_bind     complement(418..439)
                     /label=CMV(-1063)
     primer_bind     complement(425..443)
                     /label=SV40-PolyA
     primer_bind     521..540
                     /label=PEGFP-C1-R
     primer_bind     523..541
                     /label=PFastBac-R
     primer_bind     complement(586..608)
                     /label=PGEX-3
     primer_bind     complement(586..608)
                     /label=PGEX4T-3
     rep_origin      complement(788..1416)
                     /direction=LEFT
                     /label=ColE1 origin
     primer_bind     complement(886..905)
                     /label=PBR322ori-F
     primer_bind     886..905
                     /label=PBR322ori-R
     primer_bind     1444..1467
                     /label=BlaRV
     primer_bind     complement(1649..1672)
                     /label=BlaM1
     primer_bind     1793..1812
                     /label=anbian-R
     CDS             complement(1863..2241)
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     complement(1985..2004)
                     /label=anbian-F
     promoter        complement(2426..2530)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2461..2482
                     /label=163-F
     promoter        complement(2467..2495)
                     /label=Amp prom
     misc_feature    2562..2611
                     /label=2XSTAT3
     misc_feature    2624..2673
                     /label=2XSTAT3
     misc_feature    2680..2729
                     /label=2XSTAT3
     misc_feature    2736..2785
                     /label=2XSTAT3
     misc_feature    2800..2948
                     /label=P-hCMVmin
     promoter        2822..2860
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2823..2842
                     /label=PIRES2-EGFP.P5'
     primer_bind     2824..2843
                     /label=pLVX-F
     primer_bind     2855..2874
                     /label=PCEP-F
     primer_bind     2857..2882
                     /label=PLNCX 5' Primer
     primer_bind     2857..2881
                     /label=PLNCX2-5'
     primer_bind     2890..2913
                     /label=PQC-seq-F
     primer_bind     2898..2920
                     /label=CMV
     primer_bind     2907..2926
                     /label=PTRE2HYG-F
     primer_bind     complement(2933..2948)
                     /label=oYW11
     primer_bind     2959..2999
                     /label=oLH486 F
     primer_bind     2979..3006
                     /label=oLH456 F
     misc_feature    2979..2983
                     /label=New Feature
     misc_feature    2979..2983
                     /label=New Feature
     primer_bind     2985..3004
                     /label=oSCL1092
     misc_feature    2992..3230
                     /label=GLP1-Fc
     CDS             3133..3225
                     /codon_start=1
                     /label=GLP-1
                     /translation="HGEGTFTSDVSSYLEGQAAKEFIAWLVKGRG"
     misc_feature    3136..3138
                     /label=A-G
     misc_feature    3178..3180
                     /label=G-E
     primer_bind     complement(3209..3245)
                     /label=oLH471 R
     misc_feature    3220..3222
                     /label=R-G
     CDS             3226..3939
                     /codon_start=1
                     /label=Fc
                     /translation="ISAMVRSDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPE
                     VTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGK
                     EYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSD
                     IAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNH
                     YTQKSLSLSPGKASGS"
     primer_bind     3226..3250
                     /label=oLH472 F
     primer_bind     3226..3249
                     /label=Hwtx-F
     primer_bind     complement(3328..3347)
                     /label=pFUSE-R
     primer_bind     complement(3920..3972)
                     /label=oLH473 R
     primer_bind     complement(3920..3972)
                     /label=oLH483 R
     CDS             3943..3990
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATTLKQAGDVEENPGP"
     primer_bind     3954..4012
                     /label=oSCL084
     primer_bind     3955..4016
                     /label=oLH458 F
     primer_bind     3989..4012
                     /label=oSCL1108
     primer_bind     3989..4012
                     /label=oSCL1314
     misc_feature    3991..4059
                     /label=Secretory (IgK)
                     /label=nonstandard type:  signal
     CDS             4078..4593
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     misc_feature    4111..4113
                     /label=cyt-STOP (TGG->TGA)
     primer_bind     complement(4572..4593)
                     /label=oLH459 R
     primer_bind     complement(join(4575..4593,1..19))
                     /label=oLH487 R
     primer_bind     complement(4580..4590)
                     /label=oSCL085
     primer_bind     complement(4580..4590)
                     /label=oSCL1109
ORIGIN
        1 ccggtgacgg tggtccaggc tgcgttggcg gcctacctat ggctaacgcc atgggacgct
       61 agttgtgaac aaggtgtgaa gagcctattg agctacataa gaatcctccg gcccctgaat
      121 gcggctaatc ccaacctcgg agcaggtggt cacaaaccag tgattggcct gtcgtaacgc
      181 gcaagtccgt ggcggaaccg actactttgg gtgtccgtgt ttccttttat tttattgtgg
      241 ctgcttatgg tgacaatcac agattgttat cataaagcga attggattgc ggccgcaaag
      301 ctagcttcga tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
      361 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
      421 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
      481 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg
      541 attatgatcc ggctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
      601 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
      661 gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga
      721 tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac
      781 catatcgggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
      841 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
      901 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
      961 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     1021 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     1081 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     1141 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     1201 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     1261 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     1321 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     1381 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     1441 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     1501 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     1561 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     1621 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     1681 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     1741 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     1801 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     1861 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     1921 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     1981 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     2041 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     2101 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     2161 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     2221 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     2281 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     2341 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     2401 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     2461 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     2521 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgtcgacatt tcccgtaaat
     2581 cgtcgagtcg acatttcccg taaatcgtcg agggcccatc gatgtcgaca tttcccgtaa
     2641 atcgtcgagt cgacatttcc cgtaaatcgt cgagggcccg tcgacatttc ccgtaaatcg
     2701 tcgagtcgac atttcccgta aatcgtcgaa tcgatgtcga catttcccgt aaatcgtcga
     2761 gtcgacattt cccgtaaatc gtcgaacgcg tcctgcaggt cgagctcggt acccgggtcg
     2821 aggtaggcgt gtacggtggg aggcctatat aagcagagct cgtttagtga accgtcagat
     2881 cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg accgatccag
     2941 cctccgcgga attcgagctc gcccggggat cctctagaaa gcttcgccac catgaagatc
     3001 atcctgtggc tgtgtgtgtt cggcctgttc ctggccaccc tgttccccat cagctggcag
     3061 atgcccgtgg agtccggcct gtcctccgag gactccgcca gctccgagag cttcgccaag
     3121 cgcatcaagc gccacggcga gggcaccttc accagcgacg tgagcagcta cctggagggc
     3181 caggccgcca aggagttcat cgcctggctg gtgaagggcc gcggcatatc ggccatggtt
     3241 agatctgaca aaactcacac atgcccaccg tgcccagcac ctgaactcct ggggggaccg
     3301 tcagtcttcc tcttcccccc aaaacccaag gacaccctca tgatctcccg gacccctgag
     3361 gtcacatgcg tggtggtgga cgtgagccac gaagaccctg aggtcaagtt caactggtac
     3421 gtggacggcg tggaggtgca taatgccaag acaaagccgc gggaggagca gtacaacagc
     3481 acgtaccgtg tggtcagcgt cctcaccgtc ctgcaccagg actggctgaa tggcaaggag
     3541 tacaagtgca aggtctccaa caaagccctc ccagccccca tcgagaaaac catctccaaa
     3601 gccaaagggc agccccgaga accacaggtg tacaccctgc ccccatcccg ggaggagatg
     3661 accaagaacc aggtcagcct gacctgcctg gtcaaaggct tctatcccag cgacatcgcc
     3721 gtggagtggg agagcaatgg gcagccggag aacaactaca agaccacgcc tcccgtgctg
     3781 gactccgacg gctccttctt cctctacagc aagctcaccg tggacaagag caggtggcag
     3841 caggggaacg tcttctcatg ctccgtgatg cacgaggctc tgcacaacca ctacacgcag
     3901 aagagcctct ccctgtctcc gggtaaagcc agcggaagtg gagcaaccac gctgaagcag
     3961 gcaggcgacg tggaggagaa tcctggaccc atgactagtg agacagacac actcctgcta
     4021 tgggtactgc tgctctgggt tccaggttcc actggtgacg ctagtggtgg ttctggtatg
     4081 gtcttcacac tcgaagattt cgttggggac tggcgacaga cagccggcta caacctggac
     4141 caagtccttg aacagggagg tgtgtccagt ttgtttcaga atctcggggt gtccgtaact
     4201 ccgatccaaa ggattgtcct gagcggtgaa aatgggctga agatcgacat ccatgtcatc
     4261 atcccgtatg aaggtctgag cggcgaccaa atgggccaga tcgaaaaaat ttttaaggtg
     4321 gtgtaccctg tggatgatca tcactttaag gtgatcctgc actatggcac actggtaatc
     4381 gacggggtta cgccgaacat gatcgactat ttcggacggc cgtatgaagg catcgccgtg
     4441 ttcgacggca aaaagatcac tgtaacaggg accctgtgga acggcaacaa aattatcgac
     4501 gagcgcctga tcaaccccga cggctccctg ctgttccgag taaccatcaa cggagtgacc
     4561 ggctggcggc tgtgcgaacg cattctggcg tga
//