ND6-L1397-N_Q1310A_left

LOCUS       ND6-L1397-N_Q1310A_left                5462 bp DNA     circular SYN 02-APR-2026
DEFINITION  expresses the left-side half ND6-Q1310A in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    ND6-L1397-N_Q1310A_left
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5462)
  AUTHORS   Lei Z, Meng H, Liu L, Zhao H, Rao X, Yan Y, Wu H, Liu M, He A, Yi C
  TITLE     Mitochondrial base editor induces substantial nuclear off-target 
            mutations.
  JOURNAL   Nature. 2022 May 12. pii: 10.1038/s41586-022-04836-5. doi: 
            10.1038/s41586-022-04836-5.
  PUBMED    35551512
REFERENCE   2  (bases 1 to 5462)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 16, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2022 May 12. pii: 10.1038/s41586-022-04836-5. doi: 
            10.1038/s41586-022-04836-5."
FEATURES             Location/Qualifiers
     source          1..5462
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        132..335
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     286..306
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     377..396
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        377..395
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      403..412
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             502..528
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             532..558
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             562..588
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2041..2292
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     primer_bind     complement(2517..2534)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2523..2747
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(2818..2834)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2818..2834)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2831..2853)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2842..2858
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2866..2896)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2911..2932
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3049..3066)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3220..3808)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3300..3319)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3979..4839)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4602..4621
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4840..4944)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5023..5042)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(5214..5462,1..131)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
       61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat ggccctgagc
      421 agagccgttt gtggaacaag ccggcagctg gctcctgtgc ttggctacct gggcagcaga
      481 cagaagcaca gcctgccaga ctacccctat gacgtgcccg actacgccgg ctacccttat
      541 gacgtgcctg attacgccgg atacccttac gacgttccag attacgccat ggacatcgcg
      601 gacctcagga cgctgggcta cagccagcaa cagcaagaga agatcaagcc caaagtaagg
      661 agcaccgtgg cccagcacca cgaggccctg gtgggtcacg gcttcaccca cgcgcatatc
      721 gttgctctga gccaacatcc cgcagctctg ggtaccgttg cggtgaagta tcaggacatg
      781 atcgcggcac tgcctgaagc tacacacgaa gccatagtgg gcgttggcaa gcagtggagc
      841 ggtgccagag cgcttgaggc actgttgacg gtggctggcg agctgagggg accgccactg
      901 caactggaca ccggccaact gctgaagatc gccaagaggg gaggcgtgac ggcggtggag
      961 gccgtgcatg cctggaggaa cgcactgacc ggcgcgcccc tgaacctgac accagagcaa
     1021 gtagtggcta ttgcaagtca cgacggtggc aaacaagcgc tggagaccgt gcagaggctc
     1081 cttccggtgc tctgccaagc acacggtctc actccggaac aggtggtcgc aatcgcgagc
     1141 aacatcggcg gcaagcaagc ccttgagaca gtccaaagac ttttgcctgt cctttgtcag
     1201 gcgcatggcc ttacgcctga gcaagtcgtt gcgatcgcct cccacgacgg cggaaaacag
     1261 gctttggaaa ccgtgcagcg gttgctgccc gttttgtgcc aagcccacgg attgaccccc
     1321 gaacaggttg tagccatagc ttctaacatc ggaggtaagc aggcactgga aaccgtgcag
     1381 cgcctgctcc cagtactgtg tcaggctcat gggctcactc cggaacaggt ggtcgcaatc
     1441 gcgagcaacg gcggcggcaa gcaagccctt gagacagtcc aaagactttt gcctgtcctt
     1501 tgtcaggcgc atggccttac gcctgagcaa gtcgttgcga tcgcctccaa cggcggcgga
     1561 agacctgctt tggaaagcat cgtggcccag ctgagcagac ccgaccctgc cctcgccgct
     1621 ctgaccaacg accacctggt ggccctggct tgcctcgggg gcaggccagc tcttgacgcc
     1681 gtgaagaagg gccttggtgg atccggatct tacgccctgg gcccttacca gatctctgcc
     1741 cctcagctgc ccgcctacaa cggcgctacc gtgggcacat tctactacgt gaacgacgcc
     1801 ggcggcctgg aaagcaaggt gttcagcagc ggcggaccta ccccttatcc taactacgcc
     1861 aatgctggcc acgtggaagg acaaagcgcc ctgttcatga gagataacgg catcagcgag
     1921 ggcctggtct ttcacaacaa ccccgagggc acctgcggct tctgcgtgaa tatgaccgag
     1981 acactgctgc ctgaaaacgc caagatgacc gtggtgccac ctgagggaag cggcggcagc
     2041 accaacctga gcgacatcat cgagaaggaa accggcaagc agctggtgat ccaggagagc
     2101 atcctgatgc tgcctgaaga ggtggaagaa gtgatcggca acaagcctga gagcgacatc
     2161 ctggtgcaca ccgcctacga cgagtctacc gacgagaacg tgatgctgct gacaagcgat
     2221 gcccctgagt acaagccctg ggccctggtg atccaagact ccaatggcga gaacaagatc
     2281 aagatgctgt aatagaccac gatcgttatg ctgatcatac cctaatgatc ccagcaagat
     2341 aatgtcctgt cttctaagat gtgcatcaag cctggtacat actgaaaacc ctataaggtc
     2401 ctggataatt tttgtttgat tattcattga agaaacattt attttccaat tgtgtgaagt
     2461 ttttgactgt taataaaaga atctgtcaac catcaaagtt taaacccgct gatcagcctc
     2521 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     2581 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     2641 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     2701 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
     2761 aagaaccagc tggggctcaa taccgtcgac ctctagctag agcttggcgt aatcatggtc
     2821 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     2881 aagcataaag tgtaaagcct agggtgccta atgagtgagc taactcacat taattgcgtt
     2941 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     3001 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     3061 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     3121 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     3181 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     3241 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     3301 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     3361 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     3421 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     3481 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     3541 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     3601 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     3661 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     3721 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     3781 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     3841 cactcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     3901 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     3961 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     4021 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     4081 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     4141 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     4201 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     4261 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     4321 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     4381 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     4441 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     4501 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     4561 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     4621 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     4681 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     4741 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     4801 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     4861 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     4921 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg
     4981 atcgggagat cgatctcccg atcccctagg gtcgactctc agtacaatct gctctgatgc
     5041 cgcatagtta agccagtagc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
     5101 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct
     5161 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg
     5221 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
     5281 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     5341 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     5401 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     5461 tc
//