pCAMBIA1301-35S-GFP-AtKAN1

LOCUS      pCAMBIA1301-35S-GFP-AtKAN1           11306 bp DNA     circular SYN 27-FEB-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11306)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 25, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..11306
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1070..1699
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2128..3201
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      3267..3461
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     misc_feature    3805..3945
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(4131..4719)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4806..5600)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    6025..6049
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    6127..6301
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(6341..7366)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(7434..8111)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     protein_bind    8302..8323
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8338..8368
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8376..8392
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8400..8416
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        8432..8777
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             8799..9515
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     gene            9522..10733
                     /label=AT5G16560
     terminator      10777..11029
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    11051..11075
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ctgccctttt cctggcgttt tcttgtcgcg tgttttagtc gcataaagta gaatacttgc
       61 gactagaacc ggagacatta cgccatgaac aagagcgccg ccgctggcct gctgggctat
      121 gcccgcgtca gcaccgacga ccaggacttg accaaccaac gggccgaact gcacgcggcc
      181 ggctgcacca agctgttttc cgagaagatc accggcacca ggcgcgaccg cccggagctg
      241 gccaggatgc ttgaccacct acgccctggc gacgttgtga cagtgaccag gctagaccgc
      301 ctggcccgca gcacccgcga cctactggac attgccgagc gcatccagga ggccggcgcg
      361 ggcctgcgta gcctggcaga gccgtgggcc gacaccacca cgccggccgg ccgcatggtg
      421 ttgaccgtgt tcgccggcat tgccgagttc gagcgttccc taatcatcga ccgcacccgg
      481 agcgggcgcg aggccgccaa ggcccgaggc gtgaagtttg gcccccgccc taccctcacc
      541 ccggcacaga tcgcgcacgc ccgcgagctg atcgaccagg aaggccgcac cgtgaaagag
      601 gcggctgcac tgcttggcgt gcatcgctcg accctgtacc gcgcacttga gcgcagcgag
      661 gaagtgacgc ccaccgaggc caggcggcgc ggtgccttcc gtgaggacgc attgaccgag
      721 gccgacgccc tggcggccgc cgagaatgaa cgccaagagg aacaagcatg aaaccgcacc
      781 aggacggcca ggacgaaccg tttttcatta ccgaagagat cgaggcggag atgatcgcgg
      841 ccgggtacgt gttcgagccg cccgcgcacg tctcaaccgt gcggctgcat gaaatcctgg
      901 ccggtttgtc tgatgccaag ctggcggcct ggccggccag cttggccgct gaagaaaccg
      961 agcgccgccg tctaaaaagg tgatgtgtat ttgagtaaaa cagcttgcgt catgcggtcg
     1021 ctgcgtatat gatgcgatga gtaaataaac aaatacgcaa ggggaacgca tgaaggttat
     1081 cgctgtactt aaccagaaag gcgggtcagg caagacgacc atcgcaaccc atctagcccg
     1141 cgccctgcaa ctcgccgggg ccgatgttct gttagtcgat tccgatcccc agggcagtgc
     1201 ccgcgattgg gcggccgtgc gggaagatca accgctaacc gttgtcggca tcgaccgccc
     1261 gacgattgac cgcgacgtga aggccatcgg ccggcgcgac ttcgtagtga tcgacggagc
     1321 gccccaggcg gcggacttgg ctgtgtccgc gatcaaggca gccgacttcg tgctgattcc
     1381 ggtgcagcca agcccttacg acatatgggc caccgccgac ctggtggagc tggttaagca
     1441 gcgcattgag gtcacggatg gaaggctaca agcggccttt gtcgtgtcgc gggcgatcaa
     1501 aggcacgcgc atcggcggtg aggttgccga ggcgctggcc gggtacgagc tgcccattct
     1561 tgagtcccgt atcacgcagc gcgtgagcta cccaggcact gccgccgccg gcacaaccgt
     1621 tcttgaatca gaacccgagg gcgacgctgc ccgcgaggtc caggcgctgg ccgctgaaat
     1681 taaatcaaaa ctcatttgag ttaatgaggt aaagagaaaa tgagcaaaag cacaaacacg
     1741 ctaagtgccg gccgtccgag cgcacgcagc agcaaggctg caacgttggc cagcctggca
     1801 gacacgccag ccatgaagcg ggtcaacttt cagttgccgg cggaggatca caccaagctg
     1861 aagatgtacg cggtacgcca aggcaagacc attaccgagc tgctatctga atacatcgcg
     1921 cagctaccag agtaaatgag caaatgaata aatgagtaga tgaattttag cggctaaagg
     1981 aggcggcatg gaaaatcaag aacaaccagg caccgacgcc gtggaatgcc ccatgtgtgg
     2041 aggaacgggc ggttggccag gcgtaagcgg ctgggttgtc tgccggccct gcaatggcac
     2101 tggaaccccc aagcccgagg aatcggcgtg agcggtcgca aaccatccgg cccggtacaa
     2161 atcggcgcgg cgctgggtga tgacctggtg gagaagttga aggccgcgca ggccgcccag
     2221 cggcaacgca tcgaggcaga agcacgcccc ggtgaatcgt ggcaagcggc cgctgatcga
     2281 atccgcaaag aatcccggca accgccggca gccggtgcgc cgtcgattag gaagccgccc
     2341 aagggcgacg agcaaccaga ttttttcgtt ccgatgctct atgacgtggg cacccgcgat
     2401 agtcgcagca tcatggacgt ggccgttttc cgtctgtcga agcgtgaccg acgagctggc
     2461 gaggtgatcc gctacgagct tccagacggg cacgtagagg tttccgcagg gccggccggc
     2521 atggccagtg tgtgggatta cgacctggta ctgatggcgg tttcccatct aaccgaatcc
     2581 atgaaccgat accgggaagg gaagggagac aagcccggcc gcgtgttccg tccacacgtt
     2641 gcggacgtac tcaagttctg ccggcgagcc gatggcggaa agcagaaaga cgacctggta
     2701 gaaacctgca ttcggttaaa caccacgcac gttgccatgc agcgtacgaa gaaggccaag
     2761 aacggccgcc tggtgacggt atccgagggt gaagccttga ttagccgcta caagatcgta
     2821 aagagcgaaa ccgggcggcc ggagtacatc gagatcgagc tagctgattg gatgtaccgc
     2881 gagatcacag aaggcaagaa cccggacgtg ctgacggttc accccgatta ctttttgatc
     2941 gatcccggca tcggccgttt tctctaccgc ctggcacgcc gcgccgcagg caaggcagaa
     3001 gccagatggt tgttcaagac gatctacgaa cgcagtggca gcgccggaga gttcaagaag
     3061 ttctgtttca ccgtgcgcaa gctgatcggg tcaaatgacc tgccggagta cgatttgaag
     3121 gaggaggcgg ggcaggctgg cccgatccta gtcatgcgct accgcaacct gatcgagggc
     3181 gaagcatccg ccggttccta atgtacggag cagatgctag ggcaaattgc cctagcaggg
     3241 gaaaaaggtc gaaaaggtct ctttcctgtg gatagcacgt acattgggaa cccaaagccg
     3301 tacattggga accggaaccc gtacattggg aacccaaagc cgtacattgg gaaccggtca
     3361 cacatgtaag tgactgatat aaaagagaaa aaaggcgatt tttccgccta aaactcttta
     3421 aaacttatta aaactcttaa aacccgcctg gcctgtgcat aactgtctgg ccagcgcaca
     3481 gccgaagagc tgcaaaaagc gcctaccctt cggtcgctgc gctccctacg ccccgccgct
     3541 tcgcgtcggc ctatcgcggc cgctggccgc tcaaaaatgg ctggcctacg gccaggcaat
     3601 ctaccagggc gcggacaagc cgcgccgtcg ccactcgacc gccggcgccc acatcaaggc
     3661 accctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
     3721 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
     3781 agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt
     3841 gtatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg
     3901 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc
     3961 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     4021 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     4081 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     4141 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     4201 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     4261 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     4321 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     4381 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     4441 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     4501 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     4561 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     4621 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     4681 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     4741 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgcattct
     4801 aggtactaaa acaattcatc cagtaaaata taatatttta ttttctccca atcaggcttg
     4861 atccccagta agtcaaaaaa tagctcgaca tactgttctt ccccgatatc ctccctgatc
     4921 gaccggacgc agaaggcaat gtcataccac ttgtccgccc tgccgcttct cccaagatca
     4981 ataaagccac ttactttgcc atctttcaca aagatgttgc tgtctcccag gtcgccgtgg
     5041 gaaaagacaa gttcctcttc gggcttttcc gtctttaaaa aatcatacag ctcgcgcgga
     5101 tctttaaatg gagtgtcttc ttcccagttt tcgcaatcca catcggccag atcgttattc
     5161 agtaagtaat ccaattcggc taagcggctg tctaagctat tcgtataggg acaatccgat
     5221 atgtcgatgg agtgaaagag cctgatgcac tccgcataca gctcgataat cttttcaggg
     5281 ctttgttcat cttcatactc ttccgagcaa aggacgccat cggcctcact catgagcaga
     5341 ttgctccagc catcatgccg ttcaaagtgc aggacctttg gaacaggcag ctttccttcc
     5401 agccatagca tcatgtcctt ttcccgttcc acatcatagg tggtcccttt ataccggctg
     5461 tccgtcattt ttaaatatag gttttcattt tctcccacca gcttatatac cttagcagga
     5521 gacattcctt ccgtatcttt tacgcagcgg tatttttcga tcagtttttt caattccggt
     5581 gatattctca ttttagccat ttattatttc cttcctcttt tctacagtat ttaaagatac
     5641 cccaagaagc taattataac aagacgaact ccaattcact gttccttgca ttctaaaacc
     5701 ttaaatacca gaaaacagct ttttcaaagt tgttttcaaa gttggcgtat aacatagtat
     5761 cgacggagcc gattttgaaa ccgcggtgat cacaggcagc aacgctctgt catcgttaca
     5821 atcaacatgc taccctccgc gagatcatcc gtgtttcaaa cccggcagct tagttgccgt
     5881 tcttccgaat agcatcggta acatgagcaa agtctgccgc cttacaacgg ctctcccgct
     5941 gacgccgtcc cggactgatg ggctgcctgt atcgagtggt gattttgtgc cgagctgccg
     6001 gtcggggagc tgttggctgg ctggtggcag gatatattgt ggtgtaaaca aattgacgct
     6061 tagacaactt aataacacat tgcggacgtt tttaatgtac tgaattaacg ccgaattaat
     6121 tcgggggatc tggattttag tactggattt tggttttagg aattagaaat tttattgata
     6181 gaagtatttt acaaatacaa atacatacta agggtttctt atatgctcaa cacatgagcg
     6241 aaaccctata ggaaccctaa ttcccttatc tgggaactac tcacacatta ttatggagaa
     6301 actcgagctt gtcgatcgac agatccggtc ggcatctact ctatttcttt gccctcggac
     6361 gagtgctggg gcgtcggttt ccactatcgg cgagtacttc tacacagcca tcggtccaga
     6421 cggccgcgct tctgcgggcg atttgtgtac gcccgacagt cccggctccg gatcggacga
     6481 ttgcgtcgca tcgaccctgc gcccaagctg catcatcgaa attgccgtca accaagctct
     6541 gatagagttg gtcaagacca atgcggagca tatacgcccg gagtcgtggc gatcctgcaa
     6601 gctccggatg cctccgctcg aagtagcgcg tctgctgctc catacaagcc aaccacggcc
     6661 tccagaagaa gatgttggcg acctcgtatt gggaatcccc gaacatcgcc tcgctccagt
     6721 caatgaccgc tgttatgcgg ccattgtccg tcaggacatt gttggagccg aaatccgcgt
     6781 gcacgaggtg ccggacttcg gggcagtcct cggcccaaag catcagctca tcgagagcct
     6841 gcgcgacgga cgcactgacg gtgtcgtcca tcacagtttg ccagtgatac acatggggat
     6901 cagcaatcgc gcatatgaaa tcacgccatg tagtgtattg accgattcct tgcggtccga
     6961 atgggccgaa cccgctcgtc tggctaagat cggccgcagc gatcgcatcc atagcctccg
     7021 cgaccggttg tagaacagcg ggcagttcgg tttcaggcag gtcttgcaac gtgacaccct
     7081 gtgcacggcg ggagatgcaa taggtcaggc tctcgctaaa ctccccaatg tcaagcactt
     7141 ccggaatcgg gagcgcggcc gatgcaaagt gccgataaac ataacgatct ttgtagaaac
     7201 catcggcgca gctatttacc cgcaggacat atccacgccc tcctacatcg aagctgaaag
     7261 cacgagattc ttcgccctcc gagagctgca tcaggtcgga gacgctgtcg aacttttcga
     7321 tcagaaactt ctcgacagac gtcgcggtga gttcaggctt tttcatatct cattgccccc
     7381 cgggatctgc gaaagctcga gagagataga tttgtagaga gagactggtg atttcagcgt
     7441 gtcctctcca aatgaaatga acttccttat atagaggaag gtcttgcgaa ggatagtggg
     7501 attgtgcgtc atcccttacg tcagtggaga tatcacatca atccacttgc tttgaagacg
     7561 tggttggaac gtcttctttt tccacgatgc tcctcgtggg tgggggtcca tctttgggac
     7621 cactgtcggc agaggcatct tgaacgatag cctttccttt atcgcaatga tggcatttgt
     7681 aggtgccacc ttccttttct actgtccttt tgatgaagtg acagatagct gggcaatgga
     7741 atccgaggag gtttcccgat attacccttt gttgaaaagt ctcaatagcc ctttggtctt
     7801 ctgagactgt atctttgata ttcttggagt agacgagagt gtcgtgctcc accatgttat
     7861 cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc
     7921 tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga ggcatcttga acgatagcct
     7981 ttcctttatc gcaatgatgg catttgtagg tgccaccttc cttttctact gtccttttga
     8041 tgaagtgaca gatagctggg caatggaatc cgaggaggtt tcccgatatt accctttgtt
     8101 gaaaagtctc aatagccctt tggtcttctg agactgtatc tttgatattc ttggagtaga
     8161 cgagagtgtc gtgctccacc atgttggcaa gctgctctag ccaatacgca aaccgcctct
     8221 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
     8281 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
     8341 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
     8401 aggaaacagc tatgaccatg attacgaatt ctgagacttt tcaacaaagg gtaatatccg
     8461 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
     8521 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
     8581 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     8641 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     8701 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     8761 ttcatttgga gagaacatct agaggtaccg aaaagcttat ggtgagcaag ggcgaggagc
     8821 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
     8881 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
     8941 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg
     9001 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
     9061 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
     9121 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
     9181 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
     9241 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga
     9301 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
     9361 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc
     9421 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
     9481 ccgggatcac tctcggcatg gacgagctgt acaagggatc catgtctatg gaaggtgttt
     9541 ttctagagaa aaccaaaaca aacacaacaa ctactctccc tgatctatct ctccacatca
     9601 gtctcccaga tattcatcaa taccatcaca atgaatcttc taaagaatct tcaagaagat
     9661 cctcccaact cgaaaacaac aaccgatcgt ccaactttga actctcttta tctcatcata
     9721 accatccaac agcaagaatc ttccattgtc ctgatcgaag aacccttaat cttcctcatc
     9781 agcagcatta caacaaccct atcatcaatg gtgttcatca aagggtcgat gaatccgaga
     9841 ttagtaatct ccaccgtcca attagaggca tcccggtcta tcacaaccgt tcattccctt
     9901 tccaccaaca aaactcttca ttaccttctc ttggaggagg agacatggat caaatctcaa
     9961 tcttaaactc atcttccggc tacaacaacg cttaccgatc attacaatct tccccgaggc
    10021 ttaaaggtgt tcctttgcat catcatcatc atcataatca gtatggagtc gttggatctt
    10081 cagattcgtc ttctcctcat caccataacc atcatcatca tgggatgatc agatcaagat
    10141 tcttgcctaa gatgccgaca aagcgaagca tgagagctcc aaggatgcgt tggactagta
    10201 gcctccacgc gcggtttgtt cacgctgttg agcttctagg cggccatgaa agagcaactc
    10261 caaagtcggt tcttgagctc atggatgtaa aagacttaac tttagcacat gtgaagagcc
    10321 atttgcagat gtatcgaact gttaagacca ctaacaagcc tgctgcttca tcagatgggt
    10381 caggagaaga agaaatgggc ataaatggaa acgaagttca tcatcaatca tcgacggatc
    10441 aaagggcaca atctgatgat acttctcttc atcaagaaac tgacatttct tccacacaac
    10501 ctcgttggag taactcttca cgagagacat ggccattaag taataactgc tcaagcgaca
    10561 tagatacaat gatcagaact tcatcaacat caatgatctc tcattatcaa agatccagca
    10621 ttcaaaatca ggagcaaagg tcgaatgatc aagcaaagag gtgtggaaat cttagttgtg
    10681 aaaatccaag tttggagttt acattaggca gaccagattg gcacgagaaa tgagagctcg
    10741 aatttccccg atcgggtgac cagctcgaat ttccccgatc gttcaaacat ttggcaataa
    10801 agtttcttaa gattgaatcc tgttgccggt cttgcgatga ttatcatata atttctgttg
    10861 aattacgtta agcatgtaat aattaacatg taatgcatga cgttatttat gagatgggtt
    10921 tttatgatta gagtcccgca attatacatt taatacgcga tagaaaacaa aatatagcgc
    10981 gcaaactagg ataaattatc gcgcgcggtg tcatctatgt tactagatcg ggaattaaac
    11041 tatcagtgtt tgacaggata tattggcggg taaacctaag agaaaagagc gtttattaga
    11101 ataatcggat atttaaaagg gcgtgaaaag gtttatccgt tcgtccattt gtatgtgcat
    11161 gccaaccaca gggttcccct cgggatcaaa gtactttgat ccaacccctc cgctgctata
    11221 gtgcagtcgg cttctgacgt tcagtgcagc cgtcttctga aaacgacatg tcgcacaagt
    11281 cctaagttac gcgacaggct gccgcc
//