| 载体名称: | RSF1010 |
|---|---|
| 生长条件： | 37°C 1 day(s) |
| 生物安全等级: | 1级 |
| 宿主菌: | |
| 载体类型: | |
| 复制子: | |
| 抗性: | 链霉素、sul |
| 启动子: | |
| 建议宿主菌: | |
| 载体大小（kb）: | 8.9 |
| 多克隆位点: | 否 |
| 参考文献: |
P110
Nano, F. E., W. D. Shepherd, M. M. Watkins, S. A. Kuhl, and S. Kaplan. 1984. Broad-host-range plasmid vector for the in vitor construction of transcriptionaltranslational lac fusions. Gene 34: 219-226.
P116
Bagdasarian, M. M., E. Amann, R. Lurz, B. Ruckert, and M. Bagdasarina. 1983. Activity of the hybrid trp-lac(tac) promoter of Escherichia in Pseudomonas putida : construction of broad-range, controlled vectors. Gene 26: 273-282.
P46
Bagdasarian, M., R. Lurz, F. C. H. Franklin, M. M. Bagdasarina, J. Frey, and K. N. Timmis. 1981. Specific-purpose plasmid cloning vectors. II. Broad host range, high copy number, RSF1010-derived vector system for gene cloning in Pseudomonas. Gene 16: 237-247.
|
买家导航
RSF1010载体质粒基本信息
RSF1010质粒图谱载体图谱和RSF1010载体序列质粒序列多克隆位点信息
RSF1010质粒载体简介
RSF1010质粒序列载体序列
LOCUS RSF1010 8684 bp DNA SYN
DEFINITION RSF1010
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..8684
/organism="RSF1010"
/mol_type="other DNA"
CDS 375..866
/label="ORF frame 3"
/translation="MGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLP
DEDKSTPLHDLLARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLG
RLGTADRYADLALMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPL
TWG*"
CDS 866..1702
/label="ORF frame 2"
/translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
SY*"
CDS complement(1674..2213)
/label="ORF frame 1"
/translation="MPHVAARTASRDRDTGRYQSHRPEQTLLYQIVDEYYPAFAALMA
EQGKELPGYVQREFEEFLQCGRLEHGFLRVRCESCHAEHLVAFSCKRRGFCPSCGARR
MAESAALLVDEVLPEQPMRQWVLSFPFQLRFLFGVVCGKGRNPTLRLWPAIFSGEIDV
FPGDRRSRLISSMTSVAPA*"
CDS complement(3121..3777)
/label="ORF frame 3"
/translation="MVGPCLAGLLKPCLGLERFGLLCPALLRGLAVVPLEPLSGGPLD
AVIDPLGDHQVAVRVLAATGMDGQRIRQALGTGQVLGELGRQRLLLVEGQLDRQGKFD
LLEQPPIGAFIQVGSIPVVGGPLDELRHVPGFGVQDFIHVAGILAFALDVVGLGPGRL
AARPAAGFRRKVINRHAASLLLLLFGSMQWPSESAPPEGWPLGQFLEEKPVSAPSPTK
*"
CDS 3250..5379
/label="ORF frame 1"
/translation="MAIYHLTAKTGSRSGGQSARAKADYIQREGKYARDMDEVLHAES
GHMPEFVERPADYWDAADLYERANGRLFKEVEFALPVELTLDQQKALASEFAQHLTGA
ERLPYTLAIHAGGGENPHCHLMISERINDGIERPAAQWFKRYNGKTPEKGGAQKTEAL
KPKAWLEQTREAWADHANRALERAGHDARIDHRTLEAQGIERLPGVHLGPNVVEMEGR
GIRTDRADVALNIDTANAQIIDLQEYREAIDHERNRQSEEIQRHQRVSGADRTAGPEH
GDTGRRSPAGHEPDPAGQRGAGGGVAESPAPDRGGMGGAGQRVAGGSRRGEQRRAERP
ERVAGVALEAMANRDAGFHDAYGGAADRIVALARPDATDNRGRLDLAALGGPMKNDRT
LQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVLQNTPWLKRMNAQGNDVYIR
PAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKNYQAWVKVADAAGGELRGQ
IARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQPWVLLRESKGKTATAGP
ALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALDEYRSEMAGLVKRFGDD
LSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEADYIERTVSKVMGLPSV
QLARAELARAPAPRQRGMDRGGPDFSM*"
CDS 4835..5479
/label="ORF frame 2"
/translation="MAAWRASPTARTSTPPAPVISRGCCCVNPRARPPPLARRWCSRL
ASRSSRPSGSRRRPAGWPASNCPSGSLAATGARRWTSTAARWPGWSSASVMTSASATL
SPRRSWPAGAAVPRKSARPWPRPAQRWQSASPATKRITSSAPSARSWVCPASSLRGPS
WHGHRHPASEAWTGAGQISACSACVGTHACYTMSTHAQKGVLWNTKKALQGRST*"
CDS complement(5887..6654)
/label="ORF frame 3"
/translation="MPPEPPISERCAIVGLAHTEADEEPALVVVHTPFLGLGAGHARQ
VGLPADVIDQYRAAPAGLLLVACAHHGRALAGMVQEHDRAPGIGGDGLHATDDLGHGA
AGVFFLDVEPAQRVQHHQAAALGGALEAVEPLRGHDVGQAADQRLDQQAVSHGLPFLG
AEVRPKGVQAVMNGGGRVFGGQVDHRAGGQFAHLQQIRPACNLCGQLQGQHGFTGTTG
RHQRPDRTGHHVGQNVVQRWRRCCERLQNIDRLMGSH*"
CDS 5890..6729
/label="ORF frame 1"
/translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKS
MLALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAV
ADGLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVI
GRMEAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSA
EAEEWGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPR
GEA*"
CDS 6617..7567
/label="ORF frame 2"
/translation="MAHRSLIGGSGGMTAGCSSPPCWRGSARARGCPVVKPKNKHSLS
HVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDYGDGKRIEFSGPEPLGADDLRILQG
LVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAVTAECHVVKGSYRALAKEIGAEVD
SGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSEYASDEADGRLYVALNPLIAQAV
MGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGKTGKASIDTLCGYVWPSEASGS
TMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKAAG*"
promoter 7728..7758
/label="P5_promoter"
/translation="MAHRSLIGGSGGMTAGCSSPPCWRGSARARGCPVVKPKNKHSLS
HVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDYGDGKRIEFSGPEPLGADDLRILQG
LVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAVTAECHVVKGSYRALAKEIGAEVD
SGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSEYASDEADGRLYVALNPLIAQAV
MGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGKTGKASIDTLCGYVWPSEASGS
TMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKAAG*"
CDS 7875..8663
/label="ORF frame 3"
/translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
IDLVRHPAIPTPRLFRPTQKSRVCAGAGRAQADGIPVSLDSYQPATQAYALSRGVAYL
NDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAAL
TGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRAL
TGRGPGVSGPRHSLQSLPPPQVELTSSAHTSRAPCATGWRYWRR*"
ORIGIN
1 aactgcacat tcgggatatt tctctatatt cgcgcttcat cagaaaactg aaggaacctc
61 cattgaatcg aactaatatt ttttttggtg aatcgcattc tgactggttg cctgtcagag
121 gcggagaatc tggtgatttt gtttttcgac gtggtgacgg gcatgccttc gcgaaaatcg
181 cacctgcttc ccgccgcggt gagctcgctg gagagcgtga ccgcctcatt tggctcaaag
241 gtcgaggtgt ggcttgcccc gaggtcatca actggcagga ggaacaggag ggtgcatgct
301 tggtgataac ggcaattccg ggagtaccgg cggctgatct gtctggagcg gatttgctca
361 aagcgtggcc gtcaatgggg cagcaacttg gcgctgttca cagcctatcg gttgatcaat
421 gtccgtttga gcgcaggctg tcgcgaatgt tcggacgcgc cgttgatgtg gtgtcccgca
481 atgccgtcaa tcccgacttc ttaccggacg aggacaagag tacgccgctg cacgatcttt
541 tggctcgtgt cgaacgagag ctaccggtgc ggctcgacca agagcgcacc gatatggttg
601 tttgccatgg tgatccctgc atgccgaact tcatggtgga ccctaaaact cttcaatgca
661 cgggtctgat cgaccttggg cggctcggaa cagcagatcg ctatgccgat ttggcactca
721 tgattgctaa cgccgaagag aactgggcag cgccagatga agcagagcgc gccttcgctg
781 tcctattcaa tgtattgggg atcgaagccc ccgaccgcga acgccttgcc ttctatctgc
841 gattggaccc tctgacttgg ggttgatgtt catgccgcct gtttttcctg ctcattggca
901 cgtttcgcaa cctgttctca ttgcggacac cttttccagc ctcgtttgga aagtttcatt
961 gccagacggg actcctgcaa tcgtcaaggg attgaaacct atagaagaca ttgctgatga
1021 actgcgcggg gccgactatc tggtatggcg caatgggagg ggagcagtcc ggttgctcgg
1081 tcgtgagaac aatctgatgt tgctcgaata tgccggggag cgaatgctct ctcacatcgt
1141 tgccgagcac ggcgactacc aggcgaccga aattgcagcg gaactaatgg cgaagctgta
1201 tgccgcatct gaggaacccc tgccttctgc ccttctcccg atccgggatc gctttgcagc
1261 tttgtttcag cgggcgcgcg atgatcaaaa cgcaggttgt caaactgact acgtccacgc
1321 ggcgattata gccgatcaaa tgatgagcaa tgcctcggaa ctgcgtgggc tacatggcga
1381 tctgcatcat gaaaacatca tgttctccag tcgcggctgg ctggtgatag atcccgtcgg
1441 tctggtcggt gaagtgggct ttggcgccgc caatatgttc tacgatccgg ctgacagaga
1501 cgacctttgt ctcgatccta gacgcattgc acagatggcg gacgcattct ctcgtgcgct
1561 ggacgtcgat ccgcgtcgcc tgctcgacca ggcgtacgct tatgggtgcc tttccgcagc
1621 ttggaacgcg gatggagaag aggagcaacg cgatctagct atcgcggccg cgatcaagca
1681 ggtgcgacag acgtcatact agatatcaag cgacttctcc tatcccctgg gaacacatca
1741 atctcaccgg agaatatcgc tggccaaagc cttagcgtag gattccgccc cttcccgcaa
1801 acgaccccaa acaggaaacg cagctgaaac gggaagctca acacccactg acgcatgggt
1861 tgttcaggca gtacttcatc aaccagcaag gcggcacttt cggccatccg ccgcgcccca
1921 cagctcgggc agaaaccgcg acgcttacag ctgaaagcga ccaggtgctc ggcgtggcaa
1981 gactcgcagc gaacccgtag aaagccatgc tccagccgcc cgcattggag aaattcttca
2041 aattcccgtt gcacatagcc cggcaattcc tttccctgct ctgccataag cgcagcgaat
2101 gccgggtaat actcgtcaac gatctgatag agaagggttt gctcgggtcg gtggctctgg
2161 taacgaccag tatcccgatc ccggctggcc gtcctggccg ccacatgagg catgttccgc
2221 gtccttgcaa tactgtgttt acatacagtc tatcgcttag cggaaagttc ttttaccctc
2281 agccgaaatg cctgccgttg ctagacattg ccagccagtg cccgtcactc ccgtactaac
2341 tgtcacgaac ccctgcaata actgtcacgc ccccctgcaa taactgtcac gaacccctgc
2401 aataactgtc acgcccccaa acctgcaaac ccagcagggg cgggggctgg cggggtgttg
2461 gaaaaatcca tccatgatta tctaagaata atccactagg cgcggttatc agcgcccttg
2521 tggggcgctg ctgcccttgc ccaatatgcc cggccagagg ccggatagct ggtctattcg
2581 ctgcgctagg ctacacaccg ccccaccgct gcgcggcagg gggaaaggcg ggcaaagccc
2641 gctaaacccc acaccaaacc ccgcagaaat acgctggagc gcttttagcc gctttagcgg
2701 cctttccccc tacccgaagg gtgggggcgc gtgtgcagcc ccgcagggcc tgtctcggtc
2761 gatcattcag cccggctcat ccttctggcg tggcggcaga ccgaacaagg cgcggtcgtg
2821 gtcgcgttca aggtacgcat ccattgccgc catgagccga tcctccggcc actcgctgct
2881 gttcaccttg gccaaaatca tggcccccac cagcaccttg cgccttgttt cgttcttgcg
2941 ctcttgctgc tgttcccttg cccgcacccg ctgaatttcg gcattgattc gcgctcgttg
3001 ttcttcgagc ttggccagcc gatccgccgc cttgttgctc cccttaacca tcttgacacc
3061 ccattgttaa tgtgctgtct cgtaggctat catggaggca cagcggcggc aatcccgacc
3121 ctactttgta ggggagggcg cacttaccgg tttctcttcg agaaactggc ctaacggcca
3181 cccttcgggc ggtgcgctct ccgagggcca ttgcatggag ccgaaaagca aaagcaacag
3241 cgaggcagca tggcgattta tcaccttacg gcgaaaaccg gcagcaggtc gggcggccaa
3301 tcggccaggg ccaaggccga ctacatccag cgcgaaggca agtatgcccg cgacatggat
3361 gaagtcttgc acgccgaatc cgggcacatg ccggagttcg tcgagcggcc cgccgactac
3421 tgggatgctg ccgacctgta tgaacgcgcc aatgggcggc tgttcaagga ggtcgaattt
3481 gccctgccgg tcgagctgac cctcgaccag cagaaggcgc tggcgtccga gttcgcccag
3541 cacctgaccg gtgccgagcg cctgccgtat acgctggcca tccatgccgg tggcggcgag
3601 aacccgcact gccacctgat gatctccgag cggatcaatg acggcatcga gcggcccgcc
3661 gctcagtggt tcaagcggta caacggcaag accccggaga agggcggggc acagaagacc
3721 gaagcgctca agcccaaggc atggcttgag cagacccgcg aggcatgggc cgaccatgcc
3781 aaccgggcat tagagcgggc tggccacgac gcccgcattg accacagaac acttgaggcg
3841 cagggcatcg agcgcctgcc cggtgttcac ctggggccga acgtggtgga gatggaaggc
3901 cggggcatcc gcaccgaccg ggcagacgtg gccctgaaca tcgacaccgc caacgcccag
3961 atcatcgact tacaggaata ccgggaggca atagaccatg aacgcaatcg acagagtgaa
4021 gaaatccaga ggcatcaacg agttagcgga gcagatcgaa ccgctggccc agagcatggc
4081 gacactggcc gacgaagccc ggcaggtcat gagccagacc cagcaggcca gcgaggcgca
4141 ggcggcggag tggctgaaag cccagcgcca gacaggggcg gcatgggtgg agctggccaa
4201 agagttgcgg gaggtagccg ccgaggtgag cagcgccgcg cagagcgccc ggagcgcgtc
4261 gcgggggtgg cactggaagc tatggctaac cgtgatgctg gcttccatga tgcctacggt
4321 ggtgctgctg atcgcatcgt tgctcttgct cgacctgacg ccactgacaa ccgaggacgg
4381 ctcgatctgg ctgcgcttgg tggcccgatg aagaacgaca ggactttgca ggccataggc
4441 cgacagctca aggccatggg ctgtgagcgc ttcgatatcg gcgtcaggga cgccaccacc
4501 ggccagatga tgaaccggga atggtcagcc gccgaagtgc tccagaacac gccatggctc
4561 aagcggatga atgcccaggg caatgacgtg tatatcaggc ccgccgagca ggagcggcat
4621 ggtctggtgc tggtggacga cctcagcgag tttgacctgg atgacatgaa agccgagggc
4681 cgggagcctg ccctggtagt ggaaaccagc ccgaagaact atcaggcatg ggtcaaggtg
4741 gccgacgccg caggcggtga acttcggggg cagattgccc ggacgctggc cagcgagtac
4801 gacgccgacc cggccagcgc cgacagccgc cactatggcc gcttggcggg cttcaccaac
4861 cgcaaggaca agcacaccac ccgcgccggt tatcagccgt gggtgctgct gcgtgaatcc
4921 aagggcaaga ccgccaccgc tggcccggcg ctggtgcagc aggctggcca gcagatcgag
4981 caggcccagc ggcagcagga gaaggcccgc aggctggcca gcctcgaact gcccgagcgg
5041 cagcttagcc gccaccggcg cacggcgctg gacgagtacc gcagcgagat ggccgggctg
5101 gtcaagcgct tcggtgatga cctcagcaag tgcgacttta tcgccgcgca gaagctggcc
5161 agccggggcc gcagtgccga ggaaatcggc aaggccatgg ccgaggccag cccagcgctg
5221 gcagagcgca agcccggcca cgaagcggat tacatcgagc gcaccgtcag caaggtcatg
5281 ggtctgccca gcgtccagct tgcgcgggcc gagctggcac gggcaccggc accccgccag
5341 cgaggcatgg acaggggcgg gccagatttc agcatgtagt gcttgcgttg gtactcacgc
5401 ctgttatact atgagtactc acgcacagaa gggggtttta tggaatacga aaaaagcgct
5461 tcagggtcgg tctacctgat caaaagtgac aagggctatt ggttgcccgg tggctttggt
5521 tatacgtcaa acaaggccga ggctggccgc ttttcagtcg ctgatatggc cagccttaac
5581 cttgacggct gcaccttgtc cttgttccgc gaagacaagc ctttcggccc cggcaagttt
5641 ctcggtgact gatatgaaag accaaaagga caagcagacc ggcgacctgc tggccagccc
5701 tgacgctgta cgccaagcgc gatatgccga gcgcatgaag gccaaaggga tgcgtcagcg
5761 caagttctgg ctgaccgacg acgaatacga ggcgctgcgc gagtgcctgg aagaactcag
5821 agcggcgcag ggcgggggta gtgaccccgc cagcgcctaa ccaccaactg cctgcaaagg
5881 aggcaatcaa tggctaccca taagcctatc aatattctgg aggcgttcgc agcagcgccg
5941 ccaccgctgg actacgtttt gcccaacatg gtggccggta cggtcggggc gctggtgtcg
6001 cccggtggtg ccggtaaatc catgctggcc ctgcaactgg ccgcacagat tgcaggcggg
6061 ccggatctgc tggaggtggg cgaactgccc accggcccgg tgatctacct gcccgccgaa
6121 gacccgccca ccgccattca tcaccgcctg cacgcccttg gggcgcacct cagcgccgag
6181 gaacggcaag ccgtggctga cggcctgctg atccagccgc tgatcggcag cctgcccaac
6241 atcatggccc cggagtggtt cgacggcctc aagcgcgccg ccgagggccg ccgcctgatg
6301 gtgctggaca cgctgcgccg gttccacatc gaggaagaaa acgccagcgg ccccatggcc
6361 caggtcatcg gtcgcatgga ggccatcgcc gccgataccg ggtgctctat cgtgttcctg
6421 caccatgcca gcaagggcgc ggccatgatg ggcgcaggcg accagcagca ggccagccgg
6481 ggcagctcgg tactggtcga taacatccgc tggcagtcct acctgtcgag catgaccagc
6541 gccgaggccg aggaatgggg tgtggacgac gaccagcgcc ggttcttcgt ccgcttcggt
6601 gtgagcaagg ccaactatgg cgcaccgttc gctgatcggt ggttcaggcg gcatgacggc
6661 ggggtgctca agcccgccgt gctggagagg cagcgcaaga gcaagggggt gccccgtggt
6721 gaagcctaag aacaagcaca gcctcagcca cgtccggcac gacccggcgc actgtctggc
6781 ccccggcctg ttccgtgccc tcaagcgggg cgagcgcaag cgcagcaagc tggacgtgac
6841 gtatgactac ggcgacggca agcggatcga gttcagcggc ccggagccgc tgggcgctga
6901 tgatctgcgc atcctgcaag ggctggtggc catggctggg cctaatggcc tagtgcttgg
6961 cccggaaccc aagaccgaag gcggacggca gctccggctg ttcctggaac ccaagtggga
7021 ggccgtcacc gctgaatgcc atgtggtcaa aggtagctat cgggcgctgg caaaggaaat
7081 cggggcagag gtcgatagtg gtggggcgct caagcacata caggactgca tcgagcgcct
7141 ttggaaggta tccatcatcg cccagaatgg ccgcaagcgg caggggtttc ggctgctgtc
7201 ggagtacgcc agcgacgagg cggacgggcg cctgtacgtg gccctgaacc ccttgatcgc
7261 gcaggccgtc atgggtggcg gccagcatgt gcgcatcagc atggacgagg tgcgggcgct
7321 ggacagcgaa accgcccgcc tgctgcacca gcggctgtgt ggctggatcg accccggcaa
7381 aaccggcaag gcttccatag ataccttgtg cggctatgtc tggccgtcag aggccagtgg
7441 ttcgaccatg cgcaagcgcc gccagcgggt gcgcgaggcg ttgccggagc tggtcgcgct
7501 gggctggacg gtaaccgagt tcgcggcggg caagtacgac atcacccggc ccaaggcggc
7561 aggctgaccc cccccactct attgtaaaca agacattttt atcttttata ttcaatggct
7621 tattttcctg ctaattggta ataccatgaa aaataccatg ctcagaaaag gcttaacaat
7681 attttgaaaa attgcctact gagcgctgcc gcacagctcc ataggccgct ttcctggctt
7741 tgcttccaga tgtatgctct tctgctcctg cagctaatgg atcaccgcaa acaggttact
7801 cgcctgggga ttccctttcg acccgagcat ccgtatgata ctcatgctcg attattatta
7861 ttatagaagc ccccatgaat aaatcgctca tcattttcgg catcgtcaac ataacctcgg
7921 acagtttctc cgatggaggc cggtatctgg cgccagacgc agccattgcg caggcgcgta
7981 agctgatggc cgagggggca gatgtgatcg acctggtccg gcatccagca atcccgacgc
8041 cgcgcctgtt tcgtccgaca cagaaatcgc gcgtatgcgc cggtgctgga cgcgctcagg
8101 cagatggcat tcccgtctcg ctcgacagtt atcaacccgc gacgcaagcc tatgccttgt
8161 cgcgtggtgt ggcctatctc aatgatattc gcggttttcc agacgctgcg ttctatccgc
8221 aattggcgaa atcatctgcc aaactcgtcg ttatgcattc ggtgcaagac gggcaggcag
8281 atcggcgcga ggcacccgct ggcgacatca tggatcacat tgcggcgttc tttgacgcgc
8341 gcatcgcggc gctgacgggt gccggtatca aacgcaaccg ccttgtcctt gatcccggca
8401 tggggttttt tctgggggct gctcccgaaa cctcgctctc ggtgctggcg cggttcgatg
8461 aattgcggct gcgcttcgat ttgccggtgc ttctgtctgt ttcgcgcaaa tcctttctgc
8521 gcgcgctcac aggccgtggt ccgggggtgt cggggccgcg acactcgctg cagagcttgc
8581 cgccgccgca ggtggagctg acttcatccg cacacacgag ccgcgcccct tgcgcgacgg
8641 gctggcggta ttggcggcgc tgaaagaaac cgcaagaatt cgtt
//
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RSF1010质粒载体应用举例
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