| 质粒名称: | pCMV-Mm.cargo(Cre) |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 174862 |
| 存储实验室: | Feng Zhang |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.1 |
| 空载体大小: |
5400 bp
|
| 完整质粒大小: | 7410 bp |
| 载体类型: | 哺乳细胞表达 |
| 载体抗性: | 氨苄青霉素，100μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | Cre flanked by MmPeg10 UTRs |
| 物种来源: | M. musculus (mouse) |
| 插入大小: | 2000 bp |
| 启动子: | CMV |
| 克隆方法: | Gibson克隆 |
| 5’测序引物 | CMV Forward |
|
文献引用方法 |
|
| 材料和方法部分: | pCMV-Mm.cargo(Cre) was a gift from Feng Zhang (Addgene plasmid # 174862 ; http://n2t.net/addgene:174862 ; RRID:Addgene_174862) |
| 参考文献部分: |
Mammalian retrovirus-like protein PEG10 packages its own mRNA and can be pseudotyped for mRNA delivery. Segel M, Lash B, Song J, Ladha A, Liu CC, Jin X, Mekhedov SL, Macrae RK, Koonin EV, Zhang F. Science. 2021 Aug 20;373(6557):882-889. doi: 10.1126/science.abg6155. 10.1126/science.abg6155 PubMed 34413232
|
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pCMV-Mm.cargo(Cre)载体质粒基本信息
pCMV-Mm.cargo(Cre)质粒图谱载体图谱和pCMV-Mm.cargo(Cre)载体序列质粒序列多克隆位点信息
载体图谱.png)
pCMV-Mm.cargo(Cre)质粒载体简介
pCMV-Mm.cargo(Cre)质粒序列载体序列
LOCUS pCMV-Mm.cargo(Cre) 7419 bp DNA circular SYN 15-DEC-2025
DEFINITION Expresses mouse Peg10 cargoRNA encoding Cre recombinase.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7419)
AUTHORS Segel M, Lash B, Song J, Ladha A, Liu CC, Jin X, Mekhedov SL, Macrae
RK, Koonin EV, Zhang F
TITLE Mammalian retrovirus-like protein PEG10 packages its own mRNA and
can be pseudotyped for mRNA delivery.
JOURNAL Science. 2021 Aug 20;373(6557):882-889. doi:
10.1126/science.abg6155.
PUBMED 34413232
REFERENCE 2 (bases 1 to 7419)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Dec 15, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science";
journalName: "Science"; date: "2021-08-20- 20"; volume: "373";
issue: "6557"; pages: "882-889"
COMMENT Sequence Label: pCMV-Mm.cargo(Cre)
FEATURES Location/Qualifiers
source 1..7419
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 1..380
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 381..584
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 535..555
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 629..648
/label=T7
/note="T7 promoter, forward primer"
promoter 629..647
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 1047..2075
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLL
SVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLP
RPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFL
GIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVER
WISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQR
YLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLED
GD"
primer_bind complement(2776..2793)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 2782..3006
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 3052..3480
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(3139..3158)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 3349..3370
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(3489..3509)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 3494..3823
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 3674..3809
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 3736..3755
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 3890..4684
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(3944..3963)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 4554..4573
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 4858..4979
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(4895..4914)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 4949..4968
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(5028..5044)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5028..5044)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(5041..5063)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 5052..5068
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(join(5076..5082,5083..5100,5101..5106))
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5121..5142
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(5259..5276)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(5430..6018)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(5510..5529)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(join(6189..6980,6981..7049))
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 6812..6831
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(7050..7154)
/gene="bla"
/label=AmpR promoter
primer_bind complement(7229..7248)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
ORIGIN
1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
661 gctagcgttt aaaaccggat ttggcgcccc ccctcctagg atctctctat ataaggcaag
721 cagtccggac tcccgataca cgcgtttcca acttgagttg gtgtgtgtcg aagaatcctg
781 accaactacg acctggggag agcagccaac cgagaaggtc caccgagcct cgcctaggtc
841 tgctgcgcgg gctgcggtcc ggagccttct ccggaccgcg gtcacccagt ggaccgggcc
901 gtcgcgggac ccctcatcct tcgtggcatc gcagaggaat cctcgtgtgg aacaggcggg
961 ttttaagaac caaaagacgc caaccacgag ggtcccagga tccagggctc cctccccagg
1021 ggagtgaagc ccctctcacc gcagccatga atttactgac cgtacaccaa aatttgcctg
1081 cattaccggt cgatgcaacg agtgatgagg ttcgcaagaa cctgatggac atgttcaggg
1141 atcgccaggc gttttctgag catacctgga aaatgcttct gtccgtttgc cggtcgtggg
1201 cggcatggtg caagttgaat aaccggaaat ggtttcccgc agaacctgaa gatgttcgcg
1261 attatcttct atatcttcag gcgcgcggtc tggcagtaaa aactatccag caacatttgg
1321 gccagctaaa catgcttcat cgtcggtccg ggctgccacg accaagtgac agcaatgctg
1381 tttcactggt tatgcggcgg atccgaaaag aaaacgttga tgccggtgaa cgtgcaaaac
1441 aggctctagc gttcgaacgc actgatttcg accaggttcg ttcactcatg gaaaatagcg
1501 atcgctgcca ggatatacgt aatctggcat ttctggggat tgcttataac accctgttac
1561 gtatagccga aattgccagg atcagggtta aagatatctc acgtactgac ggtgggagaa
1621 tgttaatcca tattggcaga acgaaaacgc tggttagcac cgcaggtgta gagaaggcac
1681 ttagcctggg ggtaactaaa ctggtcgagc gatggatttc cgtctctggt gtagctgatg
1741 atccgaataa ctacctgttt tgccgggtca gaaaaaatgg tgttgccgcg ccatctgcca
1801 ccagccagct atcaactcgc gccctggaag ggatttttga agcaactcat cgattgattt
1861 acggcgctaa ggatgactct ggtcagagat acctggcctg gtctggacac agtgcccgtg
1921 tcggagccgc gcgagatatg gcccgcgctg gagtttcaat accggagatc atgcaagctg
1981 gtggctggac caatgtaaat attgtcatga actatatccg taacctggat agtgaaacag
2041 gggcaatggt gcgcctgctg gaagatggcg attaaggcat ggacgagctg tacaagtaac
2101 atcctgcagt gctgcgtgaa acctgtcatg tcctttgtag tctctgccct caacttgatc
2161 ctgtgcagct tctcaatcta tgactgtgtg gtactggacc ttcagaggcg cacagagctc
2221 aagtcagttt tcgtcttgac tgccacttta taagttgaca ggcctgggtt ttacttgtta
2281 aaacctctca ccatctcaat cacaggctgc caagtgtctt tacaaagaag ctgatacaaa
2341 cacaggccat gctgatttct tacagaggga gagaagagga agagaagaag aaagaggagg
2401 aagaggacat gacttgccca tatgctgggc accttataaa ggaagccaga cttttcggtg
2461 cagtatggaa aggcttccgt gattctcttg ctgcacccca cgaaacttca ccaccttcaa
2521 actccatttt cacggttccg ttaattttca aggagcagca actcgactgg ttctctgcta
2581 catgaaacac ctcagcttga aaaggaagtg ctctctcaga ctgacttgtg agtgtgcctt
2641 cacattctgg tgcaaatcat gcttaagctt ggtaccgagc tcggatccac tagtccagtg
2701 tggtggaatt ctgcagatat ccagcacagt ggcggccgct cgagtctaga gggcccgttt
2761 aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
2821 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
2881 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
2941 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
3001 ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct
3061 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
3121 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
3181 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
3241 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
3301 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
3361 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
3421 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
3481 aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
3541 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
3601 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
3661 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
3721 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca
3781 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg
3841 tatatccatt ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca
3901 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
3961 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
4021 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc
4081 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
4141 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
4201 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
4261 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
4321 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
4381 gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct
4441 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
4501 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
4561 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
4621 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
4681 ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga
4741 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
4801 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac
4861 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
4921 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
4981 catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt
5041 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
5101 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
5161 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
5221 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
5281 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
5341 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
5401 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
5461 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
5521 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
5581 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
5641 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
5701 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
5761 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
5821 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
5881 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
5941 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
6001 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
6061 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
6121 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
6181 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
6241 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
6301 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
6361 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
6421 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
6481 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
6541 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
6601 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
6661 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
6721 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
6781 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
6841 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
6901 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
6961 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
7021 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
7081 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
7141 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat
7201 ctcccgatcc cctatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
7261 agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag
7321 ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt
7381 ttgcgctgct tcgcgatgta cgggccagat atacgcgtt
//
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pCMV-Mm.cargo(Cre)质粒载体应用举例
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