| 质粒名称: | pAAVLINKv1-pCALM1-Cre-3'-ABCA10-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001848 |
| 产品用途: | 3' AAVLINK plasmid for ABCA10 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | ABCA10 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv1-pCALM1-Cre-3'-ABCA10-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv1-pCALM1-Cre-3'-ABCA10-FLAG载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-3'-ABCA10-FLAG质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-3'-ABCA10-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-3'-ABCA10-FLAG质粒载体简介
pAAVLINKv1-pCALM1-Cre-3'-ABCA10-FLAG质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-3'-ABCA10-FLAG 7195 bp DNA circular SYN 10-AUG-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7195)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 10, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7195
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
/note="color: #ffe4c4"
promoter 145..265
/label=pCALM1
CDS 277..1308
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1309..1349
/label=Splicing Donor
protein_bind 1356..1389
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1390..1481
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1482..3908
/codon_start=1
/gene="ABCA10"
/gene_synonym="EST698739"
/product="ATP-binding cassette sub-family A member 10"
/label=ABCA10
/note="ATP-binding cassette A10; ATP-binding cassette,
sub-family A (ABC1), member 10"
/db_xref="GI:1789691080"
/db_xref="CCDS:CCDS11684.1"
/db_xref="GeneID:10349"
/db_xref="HGNC:HGNC:30"
/db_xref="MIM:612508"
/protein_id="NP_001364250.1"
/translation="VLCSLPETRKAVSSAALWRRQIYAVATLRFLKLRRERRALLCLLL
VLGIAFIPIILEKIMYKVTRETHCWEFSPSMYFLSLEQIPKTPLTSLLIVNNTGSNIED
LVHSLKCQDIVLEIDDFRNRNGSDDPSYNGAIIVSGDQKDYRFSVACNTKKLNCFPVLM
GIVSNALMGIFNFTELIQMESTSFSRDDIVLDLGFIDGSIFLLLITNCVSPFIGMSSIS
DYKKNVQSQLWISGLWPSAYWCGQALVDIPLYFLILFSIHLIYYFIFLGFQLSWELMFV
LVVCIIGCAVSLIFLTYVLSFIFRKWRKNNGFWSFGFFIILICVSTIMVSTQYEKLNLI
LCMIFIPSFTLLGYVMLLIQLDFMRNLDSLDNRINEVNKTILLTTLIPYLQSVIFLFVI
RCLEMKYGNEIMNKDPVFRISPRSRETHPNPEEPEEEDEDVQAERVQAANALTAPNLEE
EPVITASCLHKEYYETKKSCFSTRKKKIAIRNVSFCVKKGEVLGLLGHNGAGKSTSIKM
ITGCTKPTAGVVVLQGSRASVRQQHDNSLKFLGYCPQENSLWPKLTMKEHLELYAAVKG
LGKEDAALSISRLVEALKLQEQLKAPVKTLSEGIKRKLCFVLSILGNPSVVLLDEPFTG
MDPEGQQQMWQILQATVKNKERGTLLTTHYMSEAEAVCDRMAMMVSGTLRCIGSIQHLK
NKFGRDYLLEIKMKEPTQVEALHTEILKLFPQAAWQERYSSLMAYKLPVEDVHPLSRAF
FKLEAMKQTFNLEEYSLSQATLEQVFLELCKEQELGNVDDKIDTTVEWKLLPQEDP"
misc_feature 1870..1875
/label=SDS
misc_feature 2851..2856
/label=SDS
CDS 3915..3980
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3984..4415
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 4128..4133
/label=SDS
repeat_region 4458..4598
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4458..4587
/label=AAV2 ITR
repeat_region 4459..4599
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
rep_origin 4673..5128
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5410..5514
/gene="bla"
/label=AmpR promoter
CDS 5515..6375
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6546..7134
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
1441 tattggtctt actgacatcc actttgcctt tctctccaca ggttctttgt tctcttcctg
1501 aaacaagaaa ggctgtcagt agtgcagctc tctggagacg acaaatctat gcagtggcaa
1561 cacttcgctt cttaaagtta aggcgtgaaa ggagagctct tttgtgtttg ttactagtac
1621 ttggaattgc ttttatcccc atcattctag agaagataat gtataaagta actcgtgaaa
1681 ctcattgttg ggagttttca cccagtatgt atttcctttc tctggaacaa atcccgaaga
1741 cgcctcttac cagcctgtta atcgttaata atacaggatc aaatattgaa gacctcgtgc
1801 attcactgaa gtgtcaggat atagttttgg aaatagatga ctttagaaac agaaatggct
1861 cagatgatcc ctcctacaat ggagccatca tagtgtctgg tgaccagaag gattacagat
1921 tttctgttgc gtgtaatacc aagaaattga attgttttcc tgttcttatg ggaattgtta
1981 gcaatgccct tatgggaatt tttaacttca cggagcttat tcaaatggag agcacttcat
2041 tttctcgtga tgacatagtg ctggatcttg gttttataga tgggtccata tttttgttgt
2101 tgatcacaaa ctgcgtttct ccttttatcg gcatgagcag catcagcgat tataaaaaaa
2161 atgttcaatc ccagttatgg atttcaggcc tctggccttc agcatactgg tgtggacagg
2221 ctctggtgga cattccatta tacttcttga ttctcttttc aatacattta atttactact
2281 tcatatttct gggattccag ctttcatggg aactcatgtt tgttttggtg gtatgcataa
2341 ttggttgtgc agtttctctt atattcctca catatgtgct ttcattcatc tttcgcaagt
2401 ggagaaaaaa taatggcttt tggtcttttg gcttttttat tatcttaata tgtgtatcca
2461 caattatggt atcaactcaa tatgaaaaac tcaacttaat tttgtgcatg attttcatac
2521 cttccttcac tttgctgggg tatgtcatgt tattgatcca gctcgacttt atgagaaact
2581 tggacagtct ggacaataga ataaatgaag tcaataaaac cattctttta acaaccttaa
2641 taccatacct tcagagtgtt attttccttt ttgtcataag gtgtctggaa atgaagtatg
2701 gaaatgaaat aatgaataaa gacccagttt tcagaatctc tccacggagt agagaaactc
2761 atcccaatcc ggaagagccc gaagaagaag atgaagatgt tcaagctgaa agagtccaag
2821 cagcaaatgc actcactgct ccaaacttgg aggaggaacc agtcataact gcaagctgtt
2881 tacacaagga atattatgag acaaagaaaa gttgcttttc aacaagaaag aagaaaatag
2941 ccatcagaaa tgtttccttt tgtgttaaaa aaggtgaagt tttgggatta ctaggacaca
3001 atggagctgg taaaagtact tccattaaaa tgataactgg gtgcacaaag ccaactgcag
3061 gagtggtggt gttacaaggc agcagagcat cagtaaggca acagcatgac aacagcctca
3121 agttcttggg gtactgccct caggagaact cactgtggcc caagcttaca atgaaagagc
3181 acttggagtt gtatgcagct gtgaaaggac tgggcaaaga agatgctgct ctcagtattt
3241 cacgattggt ggaagctctt aagctccagg aacaacttaa ggctcctgtg aaaactctat
3301 cagagggaat aaagagaaag ctgtgctttg tgctgagcat cctggggaac ccatcagtgg
3361 tgcttctaga tgagccgttc accgggatgg accccgaggg gcagcagcaa atgtggcaga
3421 tacttcaggc taccgttaaa aacaaggaga ggggcaccct cttgaccacc cattacatgt
3481 cagaggctga ggctgtgtgt gaccgtatgg ccatgatggt gtcaggaacg ctaaggtgta
3541 ttggttccat tcaacatctg aaaaacaagt ttggtagaga ttatttacta gaaataaaaa
3601 tgaaagaacc tacccaggtg gaagctctcc acacagagat tttgaagctt ttcccacagg
3661 ctgcttggca ggaaagatat tcctctttaa tggcgtataa gttacctgtg gaggatgtcc
3721 accctctatc tcgggccttt ttcaagttag aggcgatgaa acagaccttc aacctggagg
3781 aatacagcct ctctcaggct accttggagc aggtattctt agaactctgt aaagagcagg
3841 agctgggaaa tgttgatgat aaaattgata caacagttga atggaaactt ctcccacagg
3901 aagacccttg tacagactac aaagaccatg acggtgatta taaagatcat gacatcgact
3961 acaaggatga cgatgacaag taactcgaga taatcaacct ctggattaca aaatttgtga
4021 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
4081 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
4141 atcctggtta gttcttgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
4201 aggggctcgg ctgttgggca ctgacaattc cgtggtgttt atttgtgaaa tttgtgatgc
4261 tattgcttta tttgtaacca tctagcttta tttgtgaaat ttgtgatgct attgctttat
4321 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt
4381 ttcaggttca gggggagatg tgggaggttt tttaaagatc tggagaccgg tgtggcacgt
4441 gcggaccgag cggccgcagg aacccctagt gatggagttg gccactccct ctctgcgcgc
4501 tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc
4561 ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc ggtattttct
4621 ccttacgcat ctgtgcggta tttcacaccg catacgtcaa agcaaccata gtacgcgccc
4681 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
4741 gccagcgcct tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
4801 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
4861 cggcacctcg accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc
4921 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
4981 ttccaaactg gaacaacact caactctatc tcgggctatt cttttgattt ataagggatt
5041 ttgccgattt cggtctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
5101 tttaacaaaa tattaacgtt tacaatttta tggtgcactc tcagtacaat ctgctctgat
5161 gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
5221 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
5281 cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta
5341 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
5401 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
5461 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
5521 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
5581 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
5641 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
5701 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
5761 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
5821 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
5881 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
5941 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
6001 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
6061 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
6121 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
6181 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
6241 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
6301 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
6361 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
6421 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
6481 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
6541 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
6601 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
6661 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
6721 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
6781 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
6841 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
6901 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
6961 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
7021 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
7081 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
7141 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgt
//
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pAAVLINKv1-pCALM1-Cre-3'-ABCA10-FLAG质粒载体应用举例
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