pAAVLINKv1-pCALM1-Cre-3'-BTAF1-FLAG

LOCUS       pAAVLINKv1-pCALM1-Cre-3'-BTAF1-FLAG             6212 bp DNA     circular SYN 10-AUG-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6212)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 11, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6212
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     promoter        145..265
                     /label=pCALM1
     CDS             277..1308
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     intron          1309..1349
                     /label=Splicing Donor
     protein_bind    1356..1389
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1390..1481
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1482..2925
                     /codon_start=1
                     /gene="BTAF1"
                     /gene_synonym="MOT1; TAF(II)170; TAF172; TAFII170"
                     /product="TATA-binding protein-associated factor 172"
                     /label=BTAF1
                     /note="TBP-associated factor 172; B-TFIID transcription 
                     factor-associated 170 kDa subunit; ATP-dependent helicase 
                     BTAF1; BTAF1 RNA polymerase II, B-TFIID transcription 
                     factor-associated, 170kDa (Mot1 homolog, S. cerevisiae)"
                     /db_xref="GI:27477070"
                     /db_xref="CCDS:CCDS7419.1"
                     /db_xref="GeneID:9044"
                     /db_xref="HGNC:HGNC:17307"
                     /db_xref="MIM:605191"
                     /protein_id="NP_003963.1"
                     /translation="LQHQVKRHNLIVASYDVVRNDIDFFRNIKFNYCILDEGHVIKNGK
                     TKLSKAVKQLTANYRIILSGTPIQNNVLELWSLFDFLMPGFLGTERQFAARYGKPILAS
                     RDARSSSREQEAGVLAMDALHRQVLPFLLRRMKEDVLQDLPPKIIQDYYCTLSPLQVQL
                     YEDFAKSRAKCDVDETVSSATLSEETEKPKLKATGHVFQALQYLRKLCNHPALVLTPQH
                     PEFKTTAEKLAVQNSSLHDIQHAPKLSALKQLLLDCGLGNGSTSESGTESVVAQHRILI
                     FCQLKSMLDIVEHDLLKPHLPSVTYLRLDGSIPPGQRHSIVSRFNNDPSIDVLLLTTHV
                     GGLGLNLTGADTVVFVEHDWNPMRDLQAMDRAHRIGQKRVVNVYRLITRGTLEEKIMGL
                     QKFKMNIANTVISQENSSLQSMGTDQLLDLFTLDKDGKAEKADTSTSGKASMKSILENL
                     SDLWDQEQYDSEYSLENFMHSLK"
     misc_feature    2422..2427
                     /label=SDS
     CDS             2932..2997
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      3001..3432
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    3145..3150
                     /label=SDS
     repeat_region   3475..3615
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    3475..3604
                     /label=AAV2 ITR
     repeat_region   3476..3616
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      3690..4145
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4427..4531
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4532..5392
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5563..6151
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
      181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
      241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
      301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
      361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
      421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
      481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
      541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
      601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
      661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
      721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
      781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
      841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
      901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
      961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
     1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
     1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
     1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
     1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
     1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
     1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
     1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
     1441 tattggtctt actgacatcc actttgcctt tctctccaca ggttacagca ccaagtaaaa
     1501 aggcacaatc taatagtggc ttcatatgat gttgtgagga atgacataga tttctttaga
     1561 aatattaaat ttaactactg cattcttgat gaaggccatg tcatcaaaaa tggaaaaaca
     1621 aagttgtcaa aagcagtaaa acaactgact gctaattata ggattattct ttctggaaca
     1681 ccaatccaga acaacgtttt ggagctgtgg tcattatttg atttcctcat gccaggattt
     1741 ttgggtactg aacgccagtt tgctgctcga tatggtaaac ctatattagc aagtagggat
     1801 gctcgaagct ccagtcgaga gcaagaagca ggtgttcttg ctatggatgc gctgcaccgc
     1861 caagtactac cgtttctttt gagaagaatg aaagaagatg ttttgcagga tcttccacct
     1921 aaaattattc aagactatta ttgtactctt agtcctctcc aggttcagct ctatgaagat
     1981 tttgctaagt ctcgtgccaa gtgtgatgtt gatgaaacag tttcttcagc tacactttct
     2041 gaagaaactg aaaaaccaaa gcttaaagct acaggccacg tattccaggc attacagtac
     2101 ttacgtaaac tgtgcaacca tccagcatta gtcttaacac ctcaacatcc agaattcaag
     2161 accactgccg aaaaactggc agttcagaat tcttctctac atgatattca acatgcccct
     2221 aagctctcag ctttgaaaca attgctgttg gactgcggtt tgggaaatgg cagcacttcc
     2281 gagagtggca cagagtctgt tgtggcccag cacaggatac tgatattctg tcagctgaaa
     2341 agcatgcttg atatagtaga gcatgatctc ctcaaacctc acttgccctc tgtcacttat
     2401 ttgagattag atggcagcat acctcctggt cagaggcatt ccattgtttc ccggtttaat
     2461 aatgatccat ctatagacgt tctgttactt accactcacg ttggtggcct gggacttaat
     2521 ttgacaggcg ctgacacagt agtatttgtg gagcatgact ggaatcctat gcgagatcta
     2581 caagccatgg accgggccca tcgcattggg cagaaacgtg tggttaacgt ataccgattg
     2641 ataaccagag gaacattgga agaaaaaata atggggttgc agaaattcaa gatgaacata
     2701 gcgaatactg ttattagcca agagaattct agtttgcaga gcatggggac tgatcagctt
     2761 cttgatctgt ttactcttga taaggatggc aaagcagaaa aagctgacac ctctacttct
     2821 gggaaagcaa gtatgaaatc aattcttgaa aacctgagtg atctttggga tcaagagcag
     2881 tatgattcag agtacagcct ggaaaatttt atgcattctc tcaagtgtac agactacaaa
     2941 gaccatgacg gtgattataa agatcatgac atcgactaca aggatgacga tgacaagtaa
     3001 ctcgagataa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     3061 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     3121 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttagtt cttgccacgg
     3181 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     3241 acaattccgt ggtgtttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatct
     3301 agctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
     3361 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggagatgtgg
     3421 gaggtttttt aaagatctgg agaccggtgt ggcacgtgcg gaccgagcgg ccgcaggaac
     3481 ccctagtgat ggagttggcc actccctctc tgcgcgctcg ctcgctcact gaggccgggc
     3541 gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc
     3601 gcagctgcct gcaggggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt
     3661 cacaccgcat acgtcaaagc aaccatagta cgcgccctgt agcggcgcat taagcgcggc
     3721 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccttag cgcccgctcc
     3781 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     3841 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     3901 tgatttgggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     3961 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     4021 ctctatctcg ggctattctt ttgatttata agggattttg ccgatttcgg tctattggtt
     4081 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac
     4141 aattttatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg
     4201 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
     4261 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
     4321 gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     4381 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     4441 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     4501 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     4561 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     4621 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     4681 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     4741 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     4801 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     4861 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     4921 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     4981 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     5041 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     5101 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     5161 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     5221 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     5281 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     5341 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     5401 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     5461 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     5521 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     5581 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     5641 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     5701 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     5761 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     5821 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     5881 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     5941 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     6001 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     6061 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     6121 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     6181 cctggccttt tgctggcctt ttgctcacat gt
//