| 质粒名称: | pTE4497 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 80339 |
| 存储实验室: | Ervin Welker |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.1 |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 4654 bp |
| 完整质粒大小: | 9728bp |
| 载体修饰: | 人细胞色素c氧化酶亚基VIII（human cytochrome C oxidase subunit VIII） |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Fn crRNA |
| 物种来源: | -- |
| 插入大小: | 41 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | 人细胞色素c氧化酶亚基VIII（N端） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | AgeI(未破坏) |
| 3' 克隆位点: | NotI (未破坏) |
| 5' 测序引物 | CMV-F |
| 3' 测序引物 | SV40pA-R |
|
文献引用方法 |
|
| 材料和方法部分: | pTE4497 was a gift from Ervin Welker (Addgene plasmid # 80339 ; http://n2t.net/addgene:80339 ; RRID:Addgene_80339) |
| 参考文献部分: | Mb- and FnCpf1 nucleases are active in mammalian cells: activities and PAM preferences of four wild-type Cpf1 nucleases and of their altered PAM specificity variants. Toth E, Czene BC, Kulcsar PI, Krausz SL, Talas A, Nyeste A, Varga E, Huszar K, Weinhardt N, Ligeti Z, Borsy AE, Fodor E, Welker E. Nucleic Acids Res. 2018 Sep 20. pii: 5103950. doi: 10.1093/nar/gky815. 10.1093/nar/gky815 PubMed 30239882 |
买家导航
pTE4497载体质粒基本信息
pTE4497质粒图谱载体图谱和pTE4497载体序列质粒序列多克隆位点信息
pTE4497质粒载体简介
pTE4497质粒序列载体序列
LOCUS pTE4497 9728 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION mammalian expression FnCpf1 nuclease and Fn crRNA.
ACCESSION .
VERSION .
KEYWORDS pTE4497
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9728)
TITLE mammalian expression MbCpf1 nuclease and Mb crRNA
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 9728)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9728
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter complement(121..361)
/label=U6 promoter
/note="RNA polymerase III promoter for human U6 snRNA"
primer_bind complement(171..190)
/label=LKO.1 5'
/note="Human U6 promoter, forward primer"
primer_bind complement(341..361)
/label=hU6-F
/note="Human U6 promoter, forward primer"
enhancer 414..793
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 794..997
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 948..968
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 1042..1061
/label=T7
/note="T7 promoter, forward primer"
promoter 1042..1060
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 5008..5055
/codon_start=1
/product="bipartite nuclear localization signal from
nucleoplasmin"
/label=nucleoplasmin NLS
/translation="KRPAATKKAGQAKKKK"
CDS 5062..5142
/codon_start=1
/product="three tandem HA epitope tags"
/label=3xHA
/translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
primer_bind complement(5216..5233)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 5222..5446
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 5492..5920
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(5579..5598)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 5789..5810
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(5929..5949)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 5934..6263
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 6114..6249
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 6176..6195
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 6330..7124
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(6384..6403)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 6994..7013
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 7298..7419
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(7335..7354)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 7389..7408
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(7468..7484)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(7468..7484)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(7481..7503)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 7492..7508
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(7516..7546)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 7561..7582
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(7699..7716)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(7870..8455)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(7950..7969)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(8626..9486)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 9249..9268
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(9487..9591)
/gene="bla"
/label=AmpR promoter
primer_bind complement(9666..9685)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
ORIGIN
1 cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg
61 cgtacaaaaa aggagacgtt cgaacgtctc catctacaac agtagaaatt acggtgtttc
121 gtcctttcca caagatatat aaagccaaga aatcgaaata ctttcaagtt acggtaagca
181 tatgatagtc cattttaaaa cataatttta aaactgcaaa ctacccaaga aattattact
241 ttctacgtca cgtattttgt actaatatct ttgtgtttac agtcaaatta attctaatta
301 tctctctaac agccttgtat cgtatatgca aatatgaagg aatcatggga aataggccct
361 cttcctgccc gaccttagat ccgaatccat atcgatatgc tagttggcgc gttgacattg
421 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
481 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
541 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
601 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
661 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
721 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
781 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
841 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
901 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
961 taggcgtgta cggtgggagg tctatataag cagagctctc tggctaacta gagaacccac
1021 tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag ctggctagcg
1081 tttaaactta agcttggtac cgccaccatg agcatctacc aggagttcgt caacaagtat
1141 tcactgagta agacactgcg gttcgagctg atcccacagg gcaagacact ggagaacatc
1201 aaggcccgag gcctgattct ggacgatgag aagcgggcaa aagactataa gaaagccaag
1261 cagatcattg ataaatacca ccagttcttt atcgaggaaa ttctgagctc cgtgtgcatc
1321 agtgaggatc tgctgcagaa ttactcagac gtgtacttca agctgaagaa gagcgacgat
1381 gacaacctgc agaaggactt caagtccgcc aaggacacca tcaagaaaca gattagcgag
1441 tacatcaagg actccgaaaa gtttaaaaat ctgttcaacc agaatctgat cgatgctaag
1501 aaaggccagg agtccgacct gatcctgtgg ctgaaacagt ctaaggacaa tgggattgaa
1561 ctgttcaagg ctaactccga tatcactgat attgacgagg cactggaaat catcaagagc
1621 ttcaagggat ggaccacata ctttaaaggc ttccacgaga accgcaagaa cgtgtactcc
1681 agcaacgaca ttcctacctc catcatctac cgaatcgtcg atgacaatct gccaaagttc
1741 ctggagaaca aggccaaata tgaatctctg aaggacaaag ctcccgaggc aattaattac
1801 gaacagatca agaaagatct ggctgaggaa ctgacattcg atatcgacta taagactagc
1861 gaggtgaacc agagggtctt ttccctggac gaggtgtttg aaatcgccaa tttcaacaat
1921 tacctgaacc agtccggcat tactaaattc aataccatca ttggcgggaa gtttgtgaac
1981 ggggagaata ccaagcgcaa gggaattaac gaatacatca atctgtatag ccagcagatc
2041 aacgacaaaa ctctgaagaa atacaagatg tctgtgctgt tcaaacagat cctgagtgat
2101 accgagtcca agtcttttgt cattgataaa ctggaagatg actcagacgt ggtcactacc
2161 atgcagagct tttatgagca gatcgccgct ttcaagacag tggaggaaaa atctattaag
2221 gaaactctga gtctgctgtt cgatgacctg aaagcccaga agctggacct gagtaagatc
2281 tacttcaaaa acgataagag tctgacagac ctgtcacagc aggtgtttga tgactattcc
2341 gtgattggga ccgccgtcct ggagtacatt acacagcaga tcgctccaaa gaacctggat
2401 aatccctcta agaaagagca ggaactgatc gctaagaaaa ccgagaaggc aaaatatctg
2461 agtctggaaa caattaagct ggcactggag gagttcaaca agcacaggga tattgacaaa
2521 cagtgccgct ttgaggaaat cctggccaac ttcgcagcca tccccatgat ttttgatgag
2581 atcgcccaga acaaagacaa tctggctcag atcagtatta agtaccagaa ccagggcaag
2641 aaagacctgc tgcaggcttc agcagaagat gacgtgaaag ccatcaagga tctgctggac
2701 cagaccaaca atctgctgca caagctgaaa atcttccata ttagtcagtc agaggataag
2761 gctaatatcc tggataaaga cgaacacttc tacctggtgt tcgaggaatg ttacttcgag
2821 ctggcaaaca ttgtccccct gtataacaag attaggaact acatcacaca gaagccttac
2881 tctgacgaga agtttaaact gaacttcgaa aatagtaccc tggccaacgg gtgggataag
2941 aacaaggagc ctgacaacac agctatcctg ttcatcaagg atgacaagta ctatctggga
3001 gtgatgaata agaaaaacaa taagatcttc gatgacaaag ccattaagga gaacaaaggg
3061 gaaggataca agaaaatcgt gtataagctg ctgcccggcg caaataagat gctgcctaag
3121 gtgttcttca gcgccaagag tatcaaattc tacaacccat ccgaggacat cctgcggatt
3181 agaaatcact caacacatac taagaacggg agcccccaga agggatatga gaaatttgag
3241 ttcaacatcg aggattgcag gaagtttatt gacttctaca agcagagcat ctccaaacac
3301 cctgaatgga aggattttgg cttccggttt tccgacacac agagatataa ctctatcgac
3361 gagttctacc gcgaggtgga aaatcagggg tataagctga cttttgagaa catttctgaa
3421 agttacatcg acagcgtggt caatcaggga aagctgtacc tgttccagat ctataacaaa
3481 gatttttcag catacagcaa gggcagacca aacctgcata cactgtactg gaaggccctg
3541 ttcgatgaga ggaatctgca ggacgtggtc tataaactga acggagaggc cgaactgttt
3601 taccggaagc agtctattcc taagaaaatc actcacccag ctaaggaggc catcgctaac
3661 aagaacaagg acaatcctaa gaaagagagc gtgttcgaat acgatctgat taaggacaag
3721 cggttcaccg aagataagtt ctttttccat tgtccaatca ccattaactt caagtcaagc
3781 ggcgctaaca agttcaacga cgagatcaat ctgctgctga aggaaaaagc aaacgatgtg
3841 cacatcctga gcattgaccg aggagagcgg catctggcct actataccct ggtggatggc
3901 aaagggaata tcattaagca ggatacattc aacatcattg gcaatgaccg gatgaaaacc
3961 aactaccacg ataaactggc tgcaatcgag aaggatagag actcagctag gaaggactgg
4021 aagaaaatca acaacattaa ggagatgaag gaaggctatc tgagccaggt ggtccatgag
4081 attgcaaagc tggtcatcga atacaatgcc attgtggtgt tcgaggatct gaacttcggc
4141 tttaagaggg ggcgctttaa ggtggaaaaa caggtctatc agaagctgga gaaaatgctg
4201 atcgaaaagc tgaattacct ggtgtttaaa gataacgagt tcgacaagac cggaggcgtc
4261 ctgagagcct accagctgac agctcccttt gaaactttca agaaaatggg aaaacagaca
4321 ggcatcatct actatgtgcc agccggattc acttccaaga tctgccccgt gaccggcttt
4381 gtcaaccagc tgtaccctaa atatgagtca gtgagcaagt cccaggaatt tttcagcaag
4441 ttcgataaga tctgttataa tctggacaag gggtacttcg agttttcctt cgattacaag
4501 aacttcggcg acaaggccgc taaggggaaa tggaccattg cctccttcgg atctcgcctg
4561 atcaactttc gaaattccga taaaaaccac aattgggaca ctagggaggt gtacccaacc
4621 aaggagctgg aaaagctgct gaaagactac tctatcgagt atggacatgg cgaatgcatc
4681 aaggcagcca tctgtggcga gagtgataag aaatttttcg ccaagctgac ctcagtgctg
4741 aatacaatcc tgcagatgcg gaactcaaag accgggacag aactggacta tctgattagc
4801 cccgtggctg atgtcaacgg aaacttcttc gacagcagac aggcacccaa aaatatgcct
4861 caggatgcag acgccaacgg ggcctaccac atcgggctga agggactgat gctgctgggc
4921 cggatcaaga acaatcagga ggggaagaag ctgaacctgg tcattaagaa cgaggaatac
4981 ttcgagtttg tccagaatag aaataacaaa aggccggcgg ccacgaaaaa ggccggccag
5041 gcaaaaaaga aaaagggatc ctacccatac gatgttccag attacgctta tccctacgac
5101 gtgcctgatt atgcataccc atatgatgtc cccgactatg cctaagaatt ctgcagatat
5161 ccagcacagt ggcggccgct cgagtctaga gggcccgttt aaacccgctg atcagcctcg
5221 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
5281 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
5341 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
5401 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
5461 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg
5521 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
5581 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
5641 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
5701 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
5761 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
5821 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
5881 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc
5941 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
6001 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
6061 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
6121 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
6181 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt
6241 ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg
6301 atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt
6361 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
6421 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
6481 ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg
6541 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
6601 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
6661 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
6721 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
6781 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
6841 tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
6901 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
6961 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
7021 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
7081 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt
7141 cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc
7201 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
7261 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa
7321 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
7381 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac
7441 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
7501 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
7561 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
7621 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
7681 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
7741 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
7801 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
7861 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
7921 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
7981 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
8041 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
8101 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
8161 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
8221 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
8281 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
8341 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
8401 tagcggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
8461 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
8521 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
8581 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
8641 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
8701 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
8761 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
8821 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
8881 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
8941 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
9001 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
9061 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
9121 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
9181 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
9241 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
9301 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
9361 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
9421 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
9481 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
9541 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
9601 ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtgcact
9661 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg
9721 ttggaggt
//
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