pGR107

LOCUS       pGR107                 10436 bp DNA     circular SYN 19-JUN-2023
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10436)
  AUTHORS   Ming
  TITLE     Direct Submission
  JOURNAL   Exported Jun 19, 2023 from SnapGene 6.0.2 to Vector NTI(R) format
            https://www.snapgene.com
COMMENT     VNTDATE|1024617600|
COMMENT     VNTDBDATE|1024617600|
COMMENT     VNTNAME|pGR107|
COMMENT     VNTAUTHORNAME|Ming|
FEATURES             Location/Qualifiers
     misc_feature    540..562
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     promoter        659..1004
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     regulatory      1710..1719
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     terminator      7664..7916
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(7923..7939)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(8106..8124)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8145..8161)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8169..8185
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8193..8223)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8238..8259
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    8498..8522
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(8613..9201)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9302..10117)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
     rep_origin      join(10408..10436,1..407)
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
ORIGIN
        1 tttttatccc cggaagcctg tggatagagg gtagttatcc acgtgaaacc gctaatgccc
       61 cgcaaagcct tgattcacgg ggctttccgg cccgctccaa aaactatcca cgtgaaatcg
      121 ctaatcaggg tacgtgaaat cgctaatcgg agtacgtgaa atcgctaata aggtcacgtg
      181 aaatcgctaa tcaaaaaggc acgtgagaac gctaatagcc ctttcagatc aacagcttgc
      241 aaacacccct cgctccggca agtagttaca gcaagtagta tgttcaatta gcttttcaat
      301 tatgaatata tatatcaatt attggtcgcc cttggcttgt ggacaatgcg ctacgcgcac
      361 cggctccgcc cgtggacaac cgcaagcggt tgcccaccgt cgagcgccag cgcctttgcc
      421 cacaacccgg cggccggccg caacagatcg ttttataaat tttttttttt gaaaaagaaa
      481 aagcccgaaa ggcggcaacc tctcgggctt ctggatttcc gatccccgga attagatctt
      541 ggcaggatat attgtggtgt aacgttctcg catgcctgca ggtcaacatg gtggagcacg
      601 acacgcttgt ctactccaaa aatatcaaag atacagtctc agaagaccaa agggcaattg
      661 agacttttca acaaagggta atatccggaa acctcctcgg attccattgc ccagctatct
      721 gtcactttat tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc catcattgcg
      781 ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa gatggacccc
      841 cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca aagcaagtgg
      901 attgatgtga tatctccact gacgtaaggg atgacgcaca atcccactat ccttcgcaag
      961 acccttcctc tatataagga agttcatttc atttggagag gagaaaacta aaccatacac
     1021 caccaacaca accaaaccca ccacgcccaa ttgttacaca cccgcttgaa aaagaaagtt
     1081 taacaaatgg ccaaggtgcg cgaggtttac caatctttta cagactccac cacaaaaact
     1141 ctcatccaag atgaggctta tagaaacatt cgccccatca tggaaaaaca caaactagct
     1201 aacccttacg ctcaaacggt tgaagcggct aatgatctag aggggttcgg catagccacc
     1261 aatccctata gcattgaatt gcatacacat gcagccgcta agaccataga gaataaactt
     1321 ctagaggtgc ttggttccat cctaccacaa gaacctgtta catttatgtt tcttaaaccc
     1381 agaaagctaa actacatgag aagaaacccg cggatcaagg acattttcca aaatgttgcc
     1441 attgaaccaa gagacgtagc caggtacccc aaggaaacaa taattgacaa actcacagag
     1501 atcacaacgg aaacagcata cattagtgac actctgcact tcttggatcc gagctacata
     1561 gtggagacat tccaaaactg cccaaaattg caaacattgt atgcgacctt agttctcccc
     1621 gttgaggcag cctttaaaat ggaaagcact cacccgaaca tatacagcct caaatacttc
     1681 ggagatggtt tccagtatat accaggcaac catggtggcg gggcatacca tcatgaattc
     1741 gctcatctac aatggctcaa agtgggaaag atcaagtgga gggaccccaa ggatagcttt
     1801 ctcggacatc tcaattacac gactgagcag gttgagatgc acacagtgac agtacagttg
     1861 caggaatcgt tcgcggcaaa ccacttgtac tgcatcagga gaggagactt gctcacaccg
     1921 gaggtgcgca ctttcggcca acctgacagg tacgtgattc caccacagat cttcctccca
     1981 aaagttcaca actgcaagaa gccgattctc aagaaaacta tgatgcagct cttcttgtat
     2041 gttaggacag tcaaggtcgc aaaaaattgt gacatttttg ccaaagtcag acaattaatt
     2101 aaatcatctg acttggacaa atactctgct gtggaactgg tttacttagt aagctacatg
     2161 gagttccttg ccgatttaca agctaccacc tgcttctcag acacactttc tggtggcttg
     2221 ctaacaaaga cccttgcacc ggtgagggct tggatacaag agaaaaagat gcagctgttt
     2281 ggtcttgagg actacgcgaa gttagtcaaa gcagttgatt tccacccggt ggatttttct
     2341 ttcaaagtgg aaacttggga cttcagattc caccccttgc aagcgtggaa agccttccga
     2401 ccaagggaag tgtcggatgt agaggaaatg gaaagtttgt tctcagatgg ggacctgctt
     2461 gattgcttca caagaatgcc agcttatgcg gtaaacgcag aggaagattt agctgcaatc
     2521 aggaaaacgc ccgagatgga tgtcggtcaa gaagttaaag agcctgcagg agacagaaat
     2581 caatactcaa accctgcaga aactttcctc aacaagctcc acaggaaaca cagtagggag
     2641 gtgaaacacc aggccgcaaa gaaagctaaa cgcctagctg aaatccagga gtcaatgaga
     2701 gctgaaggtg atgccgaacc aaatgaaata agcgggacga tgggggcaat acccagcaac
     2761 gccgaacttc ctggcacgaa tgatgccaga caagaactca cactcccaac cactaaacct
     2821 gtccctgcaa ggtgggaaga tgcttcattc acagattcta gtgtggaaga ggagcaggtt
     2881 aaactccttg gaaaagaaac cgttgaaaca gcgacgcaac aagtcatcga aggacttcct
     2941 tggaaacact ggattcctca attaaatgct gttggattca aggcgctgga aattcagagg
     3001 gataggagtg gaacaatgat catgcccatc acagaaatgg tctccgggct ggaaaaagag
     3061 gacttccctg aaggaactcc aaaagagttg gcacgagaat tgttcgctat gaacagaagc
     3121 cctgccacca tccctttgga cctgcttaga gccagagact acggcagtga tgtaaagaac
     3181 aagagaattg gtgccatcac aaagacacag gcaacgagtt ggggcgaata cttgacagga
     3241 aagatagaaa gcttaactga gaggaaagtt gcgacttgtg tcattcatgg agctggaggt
     3301 tctggaaaaa gtcatgccat ccagaaggca ttgagagaaa ttggcaaggg ctcggacatc
     3361 actgtagtcc tgccgaccaa tgaactgcgg ctagattgga gtaagaaagt gcctaacact
     3421 gagccctata tgttcaagac ctctgaaaag gcgttaattg ggggaacagg cagcatagtc
     3481 atctttgacg attactcaaa acttcctccc ggttacatag aagccttagt ctgtttctac
     3541 tctaaaatca agctaatcat tctaacagga gatagcagac aaagcgtcta ccatgaaact
     3601 gctgaggacg cctccatcag gcatttggga ccagcaacag agtacttctc aaaatactgc
     3661 cgatactatc tcaatgccac acaccgcaac aagaaagatc ttgcgaacat gcttggtgtc
     3721 tacagtgaga gaacgggagt caccgaaatc agcatgagcg ccgagttctt agaaggaatc
     3781 ccaactttgg taccctcgga tgagaagaga aagctgtaca tgggcaccgg gaggaatgac
     3841 acgttcacat acgctggatg ccaggggcta actaagccga aggtacaaat agtgttggac
     3901 cacaacaccc aagtgtgtag cgcgaatgtg atgtacacgg cactttctag agccaccgat
     3961 aggattcact tcgtgaacac aagtgcaaat tcctctgcct tctgggaaaa gttggacagc
     4021 accccttacc tcaagacttt cctatcagtg gtgagagaac aagcactcag ggagtacgag
     4081 ccggcagagg cagagccaat tcaagagcct gagccccaga cacacatgtg tgtcgagaat
     4141 gaggagtccg tgctagaaga gtacaaagag gaactcttgg aaaagtttga cagagagatc
     4201 cactctgaat cccatggtca ttcaaactgt gtccaaactg aagacacaac cattcagttg
     4261 ttttcgcatc aacaagcaaa agatgagacc ctcctctggg cgactataga tgcgcggctc
     4321 aagaccagca atcaagaaac aaacttccga gaattcctga gcaagaagga cattggggac
     4381 gttctgtttt taaactacca aaaagctatg ggtttaccca aagagcgtat tcctttttcc
     4441 caagaggtct gggaagcttg tgcccacgaa gtacaaagca agtacctcag caagtcaaag
     4501 tgcaacttga tcaatgggac tgtgagacag agcccagact tcgatgaaaa taagattatg
     4561 gtattcctca agtcgcagtg ggtcacaaag gtggaaaaac taggtctacc caagattaag
     4621 ccaggtcaaa ccatagcagc cttttaccag cagactgtga tgctttttgg aactatggct
     4681 aggtacatgc gatggttcag acaggctttc cagccaaaag aagtcttcat aaactgtgag
     4741 accacgccag atgacatgtc tgcatgggcc ttgaacaact ggaatttcag cagacctagc
     4801 ttggctaatg actacacagc tttcgaccag tctcaggatg gagccatgtt gcaatttgag
     4861 gtgctcaaag ccaaacacca ctgcatacca gaggaaatca ttcaggcata catagatatt
     4921 aagactaatg cacagatttt cctaggcacg ttatcaatta tgcgcctgac tggtgaaggt
     4981 cccacttttg atgcaaacac tgagtgcaac atagcttaca cccatacaaa gtttgacatc
     5041 ccagccggaa ctgctcaagt ttatgcagga gacgactccg cactggactg tgttccagaa
     5101 gtgaagcata gtttccacag gcttgaggac aaattactcc taaagtcaaa gcctgtaatc
     5161 acgcagcaaa agaagggcag ttggcctgag ttttgtggtt ggctgatcac accaaaaggg
     5221 gtgatgaaag acccaattaa gctccatgtt agcttaaaat tggctgaagc taagggtgaa
     5281 ctcaagaaat gtcaagattc ctatgaaatt gatctgagtt atgcctatga ccacaaggac
     5341 tctctgcatg acttgttcga tgagaaacag tgtcaggcac acacactcac ttgcagaaca
     5401 ctaatcaagt cagggagagg cactgtctca ctttcccgcc tcagaaactt tctttaaccg
     5461 ttaagttacc ttagagattt gaataagatg gatattctca tcagtagttt gaaaagttta
     5521 ggttattcta ggacttccaa atctttagat tcaggacctt tggtagtaca tgcagtagcc
     5581 ggagccggta agtccacagc cctaaggaag ttgatcctca gacacccaac attcaccgtg
     5641 catacactcg gtgtccctga caaggtgagt atcagaacta gaggcataca gaagccagga
     5701 cctattcctg agggcaactt cgcaatcctc gatgagtata ctttggacaa caccacaagg
     5761 aactctaacc aggcactttt tgctgaccct tatcaggcac cggagtttag cctagagccc
     5821 cacttctact tggaaacatc atttcgagtt ccgaggaaag tggcagattt gatagctggc
     5881 tgtggcttcg atttcgagac caactcaccg gaagaagggc acttagagat cactggcata
     5941 ttcaaagggc ccctactcgg aaaggtgata gccattgatg aggagtctga gacaacactg
     6001 tccaggcatg gtgttgagtt tgttaagccc tgccaagtga cgggacttga gttcaaagta
     6061 gtcactattg tgtctgccgc accaatagag gaaattggcc agtccacagc tttctacaac
     6121 gctatcacca ggtcaaaggg attgacatat gtccgcgcag ggccataggc tgaccgctcc
     6181 ggtcaattct gaaaaagtgt acatagtatt aggtctatca tttgctttag tttcaattac
     6241 ctttctgctt tctagaaata gcttacccca cgtcggtgac aacattcaca gcttgccaca
     6301 cggaggagct tacagagacg gcaccaaagc aatcttgtac aactccccaa atctagggtc
     6361 acgagtgagt ctacacaacg gaaagaacgc agcatttgct gccgttttgc tactgacttt
     6421 gctgatctat ggaagtaaat acatatctca acgcaatcat acttgtgctt gtggtaacaa
     6481 tcatagcagt cattagcact tccttagtga ggactgaacc ttgtgtcatc aagattactg
     6541 gggaatcaat cacagtgttg gcttgcaaac tagatgcaga aaccataagg gccattgccg
     6601 atctcaagcc actctccgtt gaacggttaa gtttccattg atactcgaaa gaggtcagca
     6661 ccagctagca tcgattcccg ggtcgaccgc cgataagctt gatagggcca ttgccgatct
     6721 caagccactc tccgttgaac ggttaagttt ccattgatac tcgaaagatg tcagcaccag
     6781 ctagcacaac acagcccata gggtcaacta cctcaactac cacaaaaact gcaggcgcaa
     6841 ctcctgccac agcttcaggc ctgttcacca tcccggatgg ggatttcttt agtacagccc
     6901 gtgccatagt agccagcaat gctgtcgcaa caaatgagga cctcagcaag attgaggcta
     6961 tttggaagga catgaaggtg cccacagaca ctatggcaca ggctgcttgg gacttagtca
     7021 gacactgtgc tgatgtagga tcatccgctc aaacagaaat gatagataca ggtccctatt
     7081 ccaacggcat cagcagagct agactggcag cagcaattaa agaggtgtgc acacttaggc
     7141 aattttgcat gaagtatgct ccagtggtat ggaactggat gttaactaac aacagtccac
     7201 ctgctaactg gcaagcacaa ggtttcaagc ctgagcacaa attcgctgca ttcgacttct
     7261 tcaatggagt caccaaccca gctgccatca tgcccaaaga ggggctcatc cggccaccgt
     7321 ctgaagctga aatgaatgct gcccaaactg ctgcctttgt gaagattaca aaggccaggg
     7381 cacaatccaa cgactttgcc agcctagatg cagctgtcac tcgaggtcgt atcactggaa
     7441 caacaaccgc tgaggctgtt gtcactctac caccaccata actacgtcta cataaccgac
     7501 gcctacccca gtttcatagt attttctggt ttgattgtat gaataatata aataaaaaaa
     7561 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     7621 aaaaaaaaaa aaaaaaaaac tagtgggtac cgcgaatttc cccgatcgtt caaacatttg
     7681 gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt
     7741 tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag
     7801 atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat
     7861 atagcgcgca aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcgaat
     7921 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     7981 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     8041 cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcctgatgag ctccagcttt
     8101 tgttcccttt agtgagggtt aattccgagc ttggcgtaat catggtcata gctgtttcct
     8161 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     8221 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     8281 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     8341 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     8401 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     8461 gaatcagggg ataacgcagg aaagaacatg aaggccttga caggatatat tggcgggtaa
     8521 actaagtcgc tgtatgtgtt tgtttgagat ctcatgtgag caaaaggcca gcaaaaggcc
     8581 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     8641 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     8701 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     8761 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     8821 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     8881 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     8941 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     9001 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
     9061 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     9121 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     9181 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     9241 tggaacgaaa actcacgtta agggattttg gtcatggtta caaccaatta accaattctg
     9301 attagaaaaa ctcatcgagc atcaaatgaa actgcaattt attcatatca ggattatcaa
     9361 taccatattt ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg aggcagttcc
     9421 ataggatggc aagatcctgg tatcggtctg cgattccgac tcgtccaaca tcaatacaac
     9481 ctattaattt cccctcgtca aaaataaggt tatcaagtga gaaatcacca tgagtgacga
     9541 ctgaatccgg tgagaatggc aaaagtttat gcatttcttt ccagacttgt tcaacaggcc
     9601 agccattacg ctcgtcatca aaatcactcg catcaaccaa accgttattc attcgtgatt
     9661 gcgcctgagc gagacgaaat acgcgatcgc tgttaaaagg acaattacaa acaggaatcg
     9721 aatgcaaccg gcgcaggaac actgccagcg catcaacaat attttcacct gaatcaggat
     9781 attcttctaa tacctggaat gctgttttcc ctgggatcgc agtggtgagt aaccatgcat
     9841 catcaggagt acggataaaa tgcttgatgg tcggaagagg cataaattcc gtcagccagt
     9901 ttagtctgac catctcatct gtaacatcat tggcaacgct acctttgcca tgtttcagaa
     9961 acaactctgg cgcatcgggc ttcccataca atccatagat tgtcgcacct gattgcccga
    10021 cattatcgcg agcccattta tacccatata aatcagcatc catgttggaa tttaatcgcg
    10081 gcctggagca agacgtttcc cgttgaatat ggctcataac accccttgta ttactgttta
    10141 tgtaagcaga cagttttatt gttcatgatg atatattttt atcttgtgca atgtaacatc
    10201 agagattttg agacacaacg tggctttgtt gaataaatcg aacttttgct gagttgaagg
    10261 atcagatcac gcatcttccc gacaacgcag accgttccgt ggcaaagcaa aagttcaaaa
    10321 tcaccaactg gtccacctac aacaaagctc tcatcaaccg tggctccctc actttctggc
    10381 tggatgatgg ggcgattcag gcgatcccca tccaacagcc cgccgtcgag cgggct
//