| 质粒名称: | pCMV-GFP、pCMVGFP |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 11153 |
| 存储实验室: | Connie Cepko |
|
载体骨架基本信息 |
|
| 载体名称: | pCAGEN |
| 载体出品公司: | Clontech |
| 空载体大小: | 3084 bp |
| 载体类型: | 哺乳细胞表达 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | CMV promoter-EGFP |
| 基因又名: | cytomegalovirus immediate early promoter |
| 插入大小: | 1400 bp |
| 启动子: | CMV |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | Spe I(已破坏) |
| 3’克隆位点: | NotI (未破坏) |
| 5’测序引物 | CMV-F |
| 3’测序引物 | SV40pA-R |
| 备注： | 本载体插入基因EGFP，来自pEGFP-N1载体。 |
|
文献引用方法 |
|
| 材料和方法部分: |
pCMV-GFP was a gift from Connie Cepko (Addgene plasmid # 11153 ; http://n2t.net/addgene:11153 ; RRID:Addgene_11153)
|
| 参考文献部分: | Electroporation and RNA interference in the rodent retina in vivo and in vitro. Matsuda T, Cepko CL. Proc Natl Acad Sci U S A. 2004 Jan 6. 101(1):16-22. 10.1073/pnas.2235688100 PubMed 14603031 |
买家导航
pCMV-GFP载体 pCMVGFP质粒基本信息
pCMV-GFP质粒图谱 pCMVGFP载体图谱和pCMV-GFP载体序列 pCMVGFP质粒序列多克隆位点信息
pCMV-GFP质粒 pCMVGFP载体简介
pCMV-GFP质粒序列 pCMVGFP载体序列
LOCUS pCMV-GFP 4479 bp ds-DNA circular SYN 29-AUG-2023
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4479)
AUTHORS biofeng.com
TITLE Direct Submission
JOURNAL Exported Tuesday, Aug 29, 2023 from SnapGene 4.3.6 to Vector NTI(R)
format
https://www.biofeng.com
COMMENT VNTDATE|1031011200|
COMMENT VNTDBDATE|1031011200|
COMMENT VNTNAME|pCMV-GFP.dna|
COMMENT VNTAUTHORNAME|biofeng.com|
FEATURES Location/Qualifiers
enhancer 58..361
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 362..565
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
misc_feature 588..668
/label=MCS
/note="multiple cloning site"
regulatory 670..679
/regulatory_class="other"
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 676..1395
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
polyA_signal 1547..1602
/label=beta-globin poly(A) signal
/note="rabbit beta-globin polyadenylation signal (Gil and
Proudfoot, 1987)"
primer_bind complement(1963..1979)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 1987..2003
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(2011..2041)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 2056..2077
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 2135..2331
/label=SV40 promoter
/note="SV40 early promoter"
rep_origin 2182..2317
/label=SV40 ori
/note="SV40 origin of replication"
polyA_signal 2337..2471
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(2710..3298)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(3469..4329)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
promoter complement(4330..4434)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 ctagtaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
61 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
121 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
181 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
241 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
301 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
361 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
421 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
481 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
541 gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct agcgctaccg
601 gactcagatc tcgagctcaa gcttcgaatt ctgcagtcga cggtaccgcg ggcccgggat
661 ccaccggtcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
721 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
781 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
841 gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac
901 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
961 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
1021 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
1081 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc
1141 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc
1201 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
1261 ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag
1321 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
1381 gagctgtaca agtaaagcgg ccgcactcct caggtgcagg ctgcctatca gaaggtggtg
1441 gctggtgtgg ccaatgccct ggctcacaaa taccactgag atctttttcc ctctgccaaa
1501 aattatgggg acatcatgaa gccccttgag catctgactt ctggctaata aaggaaattt
1561 attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag gacatatggg
1621 agggcaaatc atttaaaaca tcagaatgag tatttggttt agagtttggc aacatatgcc
1681 catatgctgg ctgccatgaa caaaggttgg ctataaagag gtcatcagta tatgaaacag
1741 ccccctgctg tccattcctt attccataga aaagccttga cttgaggtta gatttttttt
1801 atattttgtt ttgtgttatt tttttcttta acatccctaa aattttcctt acatgtttta
1861 ctagccagat ttttcctcct ctcctgacta ctcccagtca tagctgtccc tcttctctta
1921 tggagatccc tcgacctgca gcccaagctt ggcgtaatca tggtcatagc tgtttcctgt
1981 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
2041 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
2101 tttccagtcg ggaaacctgt cgtgccagcg gatccgcatc tcaattagtc agcaaccata
2161 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
2221 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
2281 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctaact
2341 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
2401 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
2461 atgtctggat ccgctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
2521 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
2581 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
2641 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
2701 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
2761 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
2821 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
2881 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
2941 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
3001 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
3061 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
3121 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
3181 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
3241 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
3301 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
3361 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
3421 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
3481 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
3541 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
3601 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
3661 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
3721 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
3781 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
3841 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
3901 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
3961 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
4021 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
4081 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
4141 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
4201 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
4261 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
4321 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
4381 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
4441 tccccgaaaa gtgccacctg ggtcgacatt gattattga
//
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