| 质粒名称: | px330-U6-ISDra2 gRNA(PciI XbaI)-CAG-ISDra2-P2A-mCherry |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002337 |
| 产品用途: | A human codon-optimized ISDra2 TnpB and chimeric guide RNA expression plasmid. |
| 存储实验室: | Guanghai Xiang lab |
|
载体骨架基本信息 |
|
| 载体名称: | px330 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CAG |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
|
插入基因信息 |
|
| 插入基因名称: |
Nuclease, mCherry
|
| 物种来源: | Synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Comprehensive assessment of activity, specificity, and safety of hypercompact TnpB systems for gene editing.
Xin C., Xiang G., Cao S., Wang Y., Yuan S., Liu X., Huo Y., Sun J., Wan X., Liu D., Hong J., Hu J., Wang H.
Genome Biol. 2026 (2026)
|
px330-U6-ISDra2 gRNA(PciI XbaI)-CAG-ISDra2-P2A-mCherry
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
px330-U6-ISDra2 gRNA(PciI XbaI)-CAG-ISDra2-P2A-mCherry载体质粒基本信息
px330-U6-ISDra2 gRNA(PciI XbaI)-CAG-ISDra2-P2A-mCherry质粒图谱载体图谱和px330-U6-ISDra2 gRNA(PciI XbaI)-CAG-ISDra2-P2A-mCherry载体序列质粒序列多克隆位点信息
px330-U6-ISDra2 gRNA(PciI XbaI)-CAG-ISDra2-P2A-mCherry质粒载体简介
px330-U6-ISDra2 gRNA(PciI XbaI)-CAG-ISDra2-P2A-mCherry质粒序列载体序列
LOCUS px330-U6-ISDra2 gRNA(PciI XbaI)-CAG-ISDra2-P2A-mCherry 6387 bp DNA circular SYN 17-MAR-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6387)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Aug 26, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6387
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin 1..589
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
promoter 669..917
/label=U6 promoter
/note="RNA polymerase III promoter for human U6 snRNA
(Domitrovich & Kunkel, 2003)"
enhancer 1136..1421
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer;
contains an 18-bp deletion relative to the standard CMV
enhancer"
promoter 1423..1699
/label=chicken beta-actin promoter
/note="contains a 21-bp deletion relative to the standard
chicken beta-actin promoter"
promoter 1544..1563
/label=chicken beta-actin promoter
/note="contains a 21-bp deletion relative to the standard
chicken beta-actin promoter"
intron 1700..1927
/label=hybrid intron
/note="hybrid between chicken beta-actin (CBA) and minute
virus of mice (MMV) introns (Gray et al., 2011)"
regulatory 1939..1948
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 1945..3228
/codon_start=1
/label=ISDra2_humanized
/translation="MAPKKKRKVGIHGVPAATSIRNKAFVVRLYPNAAQTELINRTLGS
ARFVYNHFLARRIAAYKESGKGLTYGQTSSELTLLKQAEETSWLSEVDKFALQNSLKNL
ETAYKNFFRTVKQSGKKVGFPRFRKKRTGESYRTQFTNNNIQIGEGRLKLPKLGWVKTK
GQQDIQGKILNVTVRRIHEGHYEASVLCEVEIPYLPAAPKFAAGVDVGIKDFAIVTDGV
RFKHEQNPKYYRSTLKRLRKAQQTLSRRKKGSARYGKAKTKLARIHKRIVNKRQDFLHK
LTTSLVREYEIIGTEHLKPDNMRKNRRLALSISDAGWGEFIRQLEYKAAWYGRLVSKVS
PYFPSSQLCHDCGFKNPEVKNLAVRTWTCPNCGETHDRDENAALNIRREALVAAGISDT
LNAHGGYVRPASAGNGLRSENHATLVVGS"
CDS 1951..1971
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
misc_feature 2201..2206
/label=SDS
CDS 3229..3276
/codon_start=1
/product="bipartite nuclear localization signal from
nucleoplasmin"
/label=nucleoplasmin NLS
/translation="KRPAATKKAGQAKKKK"
CDS 3286..3342
/codon_start=1
/product="2A peptide from porcine teschovirus-1
polyprotein"
/label=P2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="ATNFSLLKQAGDVEENPGP"
CDS 3343..4053
/codon_start=1
/product="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
/label=mCherry
/note="mammalian codon-optimized"
/translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
DDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
EGRHSTGGMDELYK"
misc_feature 3359..3364
/label=SDS
misc_feature 3791..3796
/label=SDS
polyA_signal 4084..4291
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
repeat_region 4300..4440
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4300..4429
/label=AAV2 ITR
rep_origin 4515..4970
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5252..5356
/gene="bla"
/label=AmpR promoter
CDS 5357..6217
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
ORIGIN
1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt ctcgaggcta
661 gcacgcgtga gggcctattt cccatgattc cttcatattt gcatatacga tacaaggctg
721 ttagagagat aattggaatt aatttgactg taaacacaaa gatattagta caaaatacgt
781 gacgtagaaa gtaataattt cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg
841 actatcatat gcttaccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
901 ggaaaggacg aaacaccgtg gggaaaccca tgaccgagac gagaacgctg cgctgaacat
961 tcggcgtgaa gcgttggtgg ctgcgggaat ctcagacacc ttaaacgctc atggaggcta
1021 tgtcagacct gcttcggcgg gcaatggtct gcgaagtgag aatcacgcga ctttagtcgt
1081 gtgaggttca atttaatcgg atatggtaag attttttaag cttgtcgacg gtacccgtta
1141 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
1201 caatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
1261 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
1321 atgacggtaa atggcccgcc tggcattgtg cccagtacat gaccttatgg gactttccta
1381 cttggcagta catctacgta ttagtcatcg ctattaccat ggtcgaggtg agccccacgt
1441 tctgcttcac tctccccatc tcccccccct ccccaccccc aattttgtat ttatttattt
1501 tttaattatt ttgtgcagcg atgggggcgg gggggggggg gggcgcgcgc caggcggggc
1561 ggggcggggc gaggggcggg gcggggcgag gcggagaggt gcggcggcag ccaatcagag
1621 cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc cctataaaaa
1681 gcgaagcgcg cggcgggcgg gagtcgctgc gcgctgcctt cgccccgtgc cccgctccgc
1741 cgccgcctcg cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg
1801 gcgggacggc ccttctcctc cgggctgtaa ttagctgagc aagaggtaag ggtttaaggg
1861 atggttggtt ggtggggtat taatgtttaa ttacctggag cacctgcctg aaatcacttt
1921 ttttcaggtt ggaccggtgc caccatggcc ccaaagaaga agcggaaggt cggtatccac
1981 ggagtcccag cagccactag tatccggaac aaggccttcg tggtgagact gtaccccaac
2041 gccgcccaga ccgagctgat caacagaacc ctcgggagcg ccaggttcgt gtacaaccac
2101 ttcctggccc ggaggatcgc cgcctacaaa gaaagcggaa agggactcac ctacggccag
2161 accagcagcg aactcaccct gctgaaacaa gctgaggaaa cctcctggct gagcgaggtg
2221 gacaagttcg cactgcagaa cagcctgaaa aacctggaga ccgcctacaa gaacttcttc
2281 agaaccgtga agcagagcgg caagaaggtg ggcttcccca gattcagaaa gaagagaacc
2341 ggcgagagct acagaaccca gttcaccaac aacaacatcc agatcggcga gggcagactg
2401 aagctgccca agctgggctg ggtgaagacc aagggccagc aggacatcca gggcaagatc
2461 ctgaacgtga ccgtgagaag aatccacgag ggccactacg aggccagcgt gctgtgcgag
2521 gtggagatcc cctacctgcc cgccgccccc aagttcgccg ccggcgtgga cgtgggcatc
2581 aaggacttcg ccatcgtgac cgacggcgtg agattcaagc acgagcagaa ccccaagtac
2641 tacagaagca ccctgaagag actgagaaag gcccagcaga ccctgagcag aagaaagaag
2701 ggcagcgcca gatacggcaa ggccaagacc aagctggcca gaatccacaa gagaatcgtg
2761 aacaagagac aggacttcct gcacaagctg accaccagcc tggtgagaga gtacgagatc
2821 atcggcaccg agcacctgaa gcccgacaac atgagaaaga acagaagact ggccctgagc
2881 atcagcgacg ccggctgggg cgagttcatc agacagctgg agtacaaggc cgcctggtac
2941 ggcagactgg tgagcaaggt gagcccctac ttccccagca gccagctgtg ccacgactgc
3001 ggcttcaaga accccgaggt gaagaacctg gccgtgagaa cctggacctg ccccaactgc
3061 ggcgagaccc acgacagaga cgagaacgcc gccctgaaca tcagaagaga ggccctggtg
3121 gccgccggca tcagcgacac cctgaacgcc cacggcggct acgtgagacc cgccagcgcc
3181 ggcaacggcc tgagaagcga gaaccacgcc accctggtgg tgggatccaa aaggccggcg
3241 gccacgaaaa aggccggcca ggcaaaaaag aaaaagggaa gcggagctac taacttcagc
3301 ctgctgaagc aggctggaga cgtggaggag aaccctggac ctatggtgag caagggcgag
3361 gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc
3421 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
3481 cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg
3541 tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac
3601 tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgatgac
3661 ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag
3721 gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaaaaccatg
3781 ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc
3841 aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac
3901 aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc
3961 acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac
4021 tccaccggcg gcatggacga gctgtacaag taagaattcc tagagctcgc tgatcagcct
4081 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
4141 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
4201 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
4261 attgggaaga gaatagcagg catgctgggg agcggccgca ggaaccccta gtgatggagt
4321 tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc
4381 gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg
4441 ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgtc
4501 aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
4561 gcgcagcgtg accgctacac ttgccagcgc cttagcgccc gctcctttcg ctttcttccc
4621 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
4681 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg
4741 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
4801 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaactcta tctcgggcta
4861 ttcttttgat ttataaggga ttttgccgat ttcggtctat tggttaaaaa atgagctgat
4921 ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt tatggtgcac
4981 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
5041 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
5101 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg
5161 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
5221 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
5281 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
5341 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
5401 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
5461 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
5521 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
5581 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
5641 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
5701 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
5761 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
5821 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
5881 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
5941 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
6001 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
6061 cggtgagcgt ggaagccgcg gtatcattgc agcactgggg ccagatggta agccctcccg
6121 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
6181 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
6241 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
6301 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
6361 ccccgtagaa aagatcaaag gatcttc
//
