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pCMVInt载体质粒基本信息
pCMVInt质粒图谱载体图谱和pCMVInt载体序列质粒序列多克隆位点信息
pCMVInt质粒载体简介
pCMVInt质粒序列载体序列
LOCUS pCMVInt 6230 bp DNA circular SYN 13-JUL-2023
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6230)
AUTHORS Biofeng
TITLE Direct Submission
JOURNAL Exported Jul 13, 2023 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6230
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin 2..457
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
CDS 745..1605
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
protein_bind complement(1707..1740)
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
rep_origin 1818..2406
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 2907..2923
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
enhancer 2953..3256
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 3257..3460
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 3576..3594
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
CDS 3687..5504
/codon_start=1
/product="integrase from phage phi-C31"
/label=phage phi-C31 integrase
/translation="MDTYAGAYDRQSRERENSSAASPATQRSANEDKAADLQREVERDG
GRFRFVGHFSEAPGTSAFGTAERPEFERILNECRAGRLNMIIVYDVSRFSRLKVMDAIP
IVSELLALGVTIVSTQEGVFRQGNVMDLIHLIMRLDASHKESSLKSAKILDTKNLQREL
GGYVGGKAPYGFELVSETKEITRNGRMVNVVINKLAHSTTPLTGPFEFEPDVIRWWWRE
IKTHKHLPFKPGSQAAIHPGSITGLCKRMDADAVPTRGETIGKKTASSAWDPATVMRIL
RDPRIAGFAAEVIYKKKPDGTPTTKIEGYRIQRDPITLRPVELDCGPIIEPAEWYELQA
WLDGRGRGKGLSRGQAILSAMDKLYCECGAVMTSKRGEESIKDSYRCRRRKVVDPSAPG
QHEGTCNVSMAALDKFVAERIFNKIRHAEGDEETLALLWEAARRFGKLTEAPEKSGERA
NLVAERADALNALEELYEDRAAGAYDGPVGRKHFRKQQAALTLRQQGAEERLAELEAAE
APKLPLDQWFPEDADADPTGPKSWWGRASVDDKRVFVGLFVDKIVVTKSTTGRGQGTPI
EKRASITWAKPPTDDDEDDAQDGTEDVAA"
promoter complement(5568..5586)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
polyA_signal 5973..6107
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
ORIGIN
1 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
61 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
121 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
181 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
241 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
301 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
361 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
421 aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat
481 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
541 ccaggtcttg gactggtgag aacggcttgc tcggcagctt cgatgtgtgc tggagggaga
601 ataaaggtct aagatgtgcg atagagggaa gtcgcattga attatgtgct gtgtagggat
661 cgctggtatc aaatatgtgt gcccacccct ggcatgagac aataaccctg ataaatgctt
721 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
781 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
841 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
901 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
961 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
1021 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
1081 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
1141 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
1201 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
1261 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
1321 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
1381 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
1441 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
1501 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
1561 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
1621 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
1681 aagatccttt ttgataatct catgccataa cttcgtataa tgtatgctat acgaagttat
1741 ggcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
1801 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
1861 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
1921 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
1981 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
2041 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
2101 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
2161 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagca ttgagaaagc
2221 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
2281 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
2341 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
2401 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
2461 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
2521 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
2581 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
2641 agagcttgca attcgcgcgt ttttcaatat tattgaagca tttatcaggg ttattgtctc
2701 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
2761 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
2821 aaaaataggc gtatacgagg ccctttcact cattaggcac cccaggcttt acactttata
2881 gcttccggct cgtataatgt gtggaattgt gagcggataa caatttcaca caggaaacag
2941 catcgtgcag gtcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
3001 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
3061 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
3121 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
3181 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
3241 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
3301 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
3361 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
3421 cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga accgtcagat
3481 cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg accgatccag
3541 cctccggact ctagcctagg cttttgcaaa aagctattta ggtgacacta tagaaggtac
3601 gcctgcaggt accggtccgg aattcccggg tcgacgagct cactagtcgt agggtcgccg
3661 acatgacaca aggggttgtg accggggtgg acacgtacgc gggtgcttac gaccgtcagt
3721 cgcgcgagcg cgaaaattcg agcgcagcaa gcccagcgac acagcgtagc gccaacgaag
3781 acaaggcggc cgaccttcag cgcgaagtcg agcgcgacgg gggccggttc aggttcgtcg
3841 ggcatttcag cgaagcgccg ggcacgtcgg cgttcgggac ggcggagcgc ccggagttcg
3901 aacgcatcct gaacgaatgc cgcgccgggc ggctcaacat gatcattgtc tatgacgtgt
3961 cgcgcttctc gcgcctgaag gtcatggacg cgattccgat tgtctcggaa ttgctcgccc
4021 tgggcgtgac gattgtttcc actcaggaag gcgtcttccg gcagggaaac gtcatggacc
4081 tgattcacct gattatgcgg ctcgacgcgt cgcacaaaga atcttcgctg aagtcggcga
4141 agattctcga cacgaagaac cttcagcgcg aattgggcgg gtacgtcggc gggaaggcgc
4201 cttacggctt cgagcttgtt tcggagacga aggagatcac gcgcaacggc cgaatggtca
4261 atgtcgtcat caacaagctt gcgcactcga ccactcccct taccggaccc ttcgagttcg
4321 agcccgacgt aatccggtgg tggtggcgtg agatcaagac gcacaaacac cttcccttca
4381 agccgggcag tcaagccgcc attcacccgg gcagcatcac ggggctttgt aagcgcatgg
4441 acgctgacgc cgtgccgacc cggggcgaga cgattgggaa gaagaccgct tcaagcgcct
4501 gggacccggc aaccgttatg cgaatccttc gggacccgcg tattgcgggc ttcgccgctg
4561 aggtgatcta caagaagaag ccggacggca cgccgaccac gaagattgag ggttaccgca
4621 ttcagcgcga cccgatcacg ctccggccgg tcgagcttga ttgcggaccg atcatcgagc
4681 ccgctgagtg gtatgagctt caggcgtggt tggacggcag ggggcgcggc aaggggcttt
4741 cccgggggca agccattctg tccgccatgg acaagctgta ctgcgagtgt ggcgccgtca
4801 tgacttcgaa gcgcggggaa gaatcgatca aggactctta ccgctgccgt cgccggaagg
4861 tggtcgaccc gtccgcacct gggcagcacg aaggcacgtg caacgtcagc atggcggcac
4921 tcgacaagtt cgttgcggaa cgcatcttca acaagatcag gcacgccgaa ggcgacgaag
4981 agacgttggc gcttctgtgg gaagccgccc gacgcttcgg caagctcact gaggcgcctg
5041 agaagagcgg cgaacgggcg aaccttgttg cggagcgcgc cgacgccctg aacgcccttg
5101 aagagctgta cgaagaccgc gcggcaggcg cgtacgacgg acccgttggc aggaagcact
5161 tccggaagca acaggcagcg ctgacgctcc ggcagcaagg ggcggaagag cggcttgccg
5221 aacttgaagc cgccgaagcc ccgaagcttc cccttgacca atggttcccc gaagacgccg
5281 acgctgaccc gaccggccct aagtcgtggt gggggcgcgc gtcagtagac gacaagcgcg
5341 tgttcgtcgg gctcttcgta gacaagatcg ttgtcacgaa gtcgactacg ggcagggggc
5401 agggaacgcc catcgagaag cgcgcttcga tcacgtgggc gaagccgccg accgacgacg
5461 acgaagacga cgcccaggac ggcacggaag acgtagcggc gtagcgagac acccggatcc
5521 ctcgaggggc ccaagcttac gcgtgcatgc gacgtcatag ctctctccct atagtgagtc
5581 gtattataag ctagcttggg atctttgtga aggaacctta cttctgtggt gtgacataat
5641 tggacaaact acctacagag atttaaagct ctaaggtaaa tataaaattt ttaagtgtat
5701 aatgtgttaa actagctgca tatgcttgct gcttgagagt tttgcttact gagtatgatt
5761 tatgaaaata ttatacacag gagctagtga ttctaattgt ttgtgtattt tagattcaca
5821 gtcccaaggc tcatttcagg cccctcagtc ctcacagtct gttcatgatc ataatcagcc
5881 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc
5941 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
6001 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
6061 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcgat cctgcattaa
6121 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg gctggcgtaa tagcgaagag
6181 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
//
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