| 质粒名称: | pGRG37 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 16667 |
| 存储实验室: | Nancy Craig |
|
载体骨架基本信息 |
|
| 载体名称: | pGRG37 |
| 载体出品公司: | -- |
| 载体抗性: | 氯霉素和氨苄青霉素 |
| 空载体大小: | 14263 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 细菌表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 低拷贝 |
| 生长条件: | 30 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | mTn7::Gateway cassette |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | Gateway克隆 |
| 5' 克隆位点: | attR1(未破坏) |
| 3' 克隆位点: | attR2(未破坏) |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pGRG37 was a gift from Nancy Craig (Addgene plasmid # 16667 ; http://n2t.net/addgene:16667 ; RRID:Addgene_16667) |
| 参考文献部分: | Fast, easy and efficient: site-specific insertion of transgenes into enterobacterial chromosomes using Tn7 without need for selection of the insertion event. McKenzie GJ, Craig NL. BMC Microbiol. 2006 . 6():39. 10.1186/1471-2180-6-39 PubMed 16646962 |
买家导航
pGRG37载体质粒基本信息
pGRG37质粒图谱载体图谱和pGRG37载体序列质粒序列多克隆位点信息
pGRG37质粒载体简介
pGRG37质粒序列载体序列
LOCUS pGRG37 14263 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pGRG37
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 14263)
AUTHORS McKenzie GJ, Craig NL
TITLE Fast, easy and efficient: site-specific insertion of transgenes into
enterobacterial chromosomes using Tn7 without need for selection of
the insertion event.
JOURNAL BMC Microbiol. 2006 . 6():39.
PUBMED 16646962
REFERENCE 2 (bases 1 to 14263)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..14263
/organism="synthetic DNA construct"
/mol_type="other DNA"
protein_bind 6..130
/gene="mutant version of attR"
/label=attR1
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
promoter 155..185
/label=lac UV5 promoter
/note="E. coli lac promoter with an ""up"" mutation"
CDS 239..919
/codon_start=1
/product="chloramphenicol acetyltransferase"
/label=CmR
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
DPAY"
primer_bind complement(305..324)
/label=CAT-R
/note="Chloramphenicol resistance gene, reverse primer"
CDS 1239..1544
/codon_start=1
/gene="ccdB"
/product="CcdB, a bacterial toxin that poisons DNA gyrase"
/label=ccdB
/note="Plasmids containing the ccdB gene cannot be
propagated in standard E. coli strains."
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
primer_bind 1423..1442
/label=ccdB-fwd
/note="ccdB gene, forward primer"
protein_bind complement(1585..1709)
/gene="mutant version of attR"
/label=attR2
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
CDS complement(2116..2994)
/codon_start=1
/gene="araC"
/product="L-arabinose regulatory protein"
/label=araC
/translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
EKVNDVAVKLS"
promoter 3021..3305
/gene="araBAD"
/label=araBAD promoter
/note="promoter of the L-arabinose operon of E. coli; the
araC regulatory gene is transcribed in the opposite
direction (Guzman et al., 1995)"
primer_bind 3228..3247
/label=pBAD Forward
/note="For vectors with E. coli araBAD promoter, forward
primer"
primer_bind complement(9996..10013)
/label=pBAD Reverse
/note="For vectors with E. coli araBAD promoter, reverse
primer"
terminator 10166..10252
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 10344..10371
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
promoter 10390..10481
/gene="bla"
/label=AmpR promoter
CDS 10482..11342
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(10700..10719)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
primer_bind complement(11471..11490)
/label=F1ori-R
/note="F1 origin, reverse primer"
oriT 11513..11622
/note="incP origin of transfer"
primer_bind complement(11660..11679)
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
rep_origin 12362..12584
/label=pSC101 ori
/note="low-copy replication origin that requires the Rep101
protein"
CDS 12632..13582
/codon_start=1
/gene="rep101 encoding an A56V mutation"
/product="temperature-sensitive version of the RepA protein
needed for replication with the pSC101 origin (Armstrong et
al., 1984)"
/label=Rep101(Ts)
/translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
RTVSFTYNQYVQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
mobile_element 14015..14180
/mobile_element_type="transposon:Tn7"
/note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
1 atcaaacaag tttgtacaaa aaagctgaac gagaaacgta aaatgatata aatatcaata
61 tattaaatta gattttgcat aaaaaacaga ctacataata ctgtaaaaca caacatatcc
121 agtcctatgg gcggccgcat taggcacccc aggctttaca ctttatgctt ccggctcgta
181 taatgtgtgg attttgagtt aggatccgtc gagattttca ggagctaagg aagctaaaat
241 ggagaaaaaa atcactggat ataccaccgt tgatatatcc caatggcatc gtaaagaaca
301 ttttgaggca tttcagtcag ttgctcaatg tacctataac cagaccgttc agctggatat
361 tacggccttt ttaaagaccg taaagaaaaa taagcacaag ttttatccgg cctttattca
421 cattcttgcc cgcctgatga atgctcatcc ggaattccgt atggcaatga aagacggtga
481 gctggtgata tgggatagtg ttcacccttg ttacaccgtt ttccatgagc aaactgaaac
541 gttttcatcg ctctggagtg aataccacga cgatttccgg cagtttctac acatatattc
601 gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt ttattgagaa
661 tatgtttttc gtctcagcca atccctgggt gagtttcacc agttttgatt taaacgtggc
721 caatatggac aacttcttcg cccccgtttt caccatgggc aaatattata cgcaaggcga
781 caaggtgctg atgccgctgg cgattcaggt tcatcatgcc gtttgtgatg gcttccatgt
841 cggcagaatg cttaatgaat tacaacagta ctgcgatgag tggcaggcgg ggcgtaatct
901 agaggatccg gcttactaaa agccagataa cagtatgcgt atttgcgcgc tgatttttgc
961 ggtataagaa tatatactga tatgtatacc cgaagtatgt caaaaagagg tatgctatga
1021 agcagcgtat tacagtgaca gttgacagcg acagctatca gttgctcaag gcatatatga
1081 tgtcaatatc tccggtctgg taagcacaac catgcagaat gaagcccgtc gtctgcgtgc
1141 cgaacgctgg aaagcggaaa atcaggaagg gatggctgag gtcgcccggt ttattgaaat
1201 gaacggctct tttgctgacg agaacagggg ctggtgaaat gcagtttaag gtttacacct
1261 ataaaagaga gagccgttat cgtctgtttg tggatgtaca gagtgatatt attgacacgc
1321 ccgggcgacg gatggtgatc cccctggcca gtgcacgtct gctgtcagat aaagtccccc
1381 gtgaacttta cccggtggtg catatcgggg atgaaagctg gcgcatgatg accaccgata
1441 tggccagtgt gccggtctcc gttatcgggg aagaagtggc tgatctcagc caccgcgaaa
1501 atgacatcaa aaacgccatt aacctgatgt tctggggaat ataaatgtca ggctccctta
1561 tacacagcca gtctgcaggt cgaccatagt gactggatat gttgtgtttt acagtattat
1621 gtagtctgtt ttttatgcaa aatctaattt aatatattga tatttatatc attttacgtt
1681 tctcgttcag ctttcttgta caaagtggtt cgatgggtca atagcggccg ccactcgagc
1741 acctaggagg cgcgccacgg ccgcgtcgac cccacgcccc tctttaatac gacgggcaat
1801 ttgcacttca gaaaatgaag agtttgcttt agccataaca aaagtccagt atgctttttc
1861 acagcataac tggactgatt tcagtttaca actattctgt ctagtttaag actttattgt
1921 catagtttag atctattttg ttcagtttaa gactttattg tccgcccaca ggccacgatg
1981 cgtccggcgt agaggatctg ctcatgtttg acagcttatc atcgatgcat aatgtgcctg
2041 tcaaatggac gaagcaggga ttctgcaaac cctatgctac tccgtcaagc cgtcaattgt
2101 ctgattcgtt accaattatg acaacttgac ggctacatca ttcacttttt cttcacaacc
2161 ggcacggaac tcgctcgggc tggccccggt gcatttttta aatacccgcg agaaatagag
2221 ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata ggcatccggg tggtgctcaa
2281 aagcagcttc gcctggctga tacgttggtc ctcgcgccag cttaagacgc taatccctaa
2341 ctgctggcgg aaaagatgtg acagacgcga cggcgacaag caaacatgct gtgcgacgct
2401 ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag cctcgcgtac
2461 ccgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc gcagtaacaa
2521 ttgctcaagc agatttatcg ccagcagctc cgaatagcgc ccttcccctt gcccggcgtt
2581 aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg ggcgaaagaa
2641 ccccgtattg gcaaatattg acggccagtt aagccattca tgccagtagg cgcgcggacg
2701 aaagtaaacc cactggtgat accattcgcg agcctccgga tgacgaccgt agtgatgaat
2761 ctctcctggc gggaacagca aaatatcacc cggtcggcaa acaaattctc gtccctgatt
2821 tttcaccacc ccctgaccgc gaatggtgag attgagaata taacctttca ttcccagcgg
2881 tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac ccgccaccag
2941 atgggcatta aacgagtatc ccggcagcag gggatcattt tgcgcttcag ccatactttt
3001 catactcccg ccattcagag aagaaaccaa ttgtccatat tgcatcagac attgccgtca
3061 ctgcgtcttt tactggctct tctcgctaac caaaccggta accccgctta ttaaaagcat
3121 tctgtaacaa agcgggacca aagccatgac aaaaacgcgt aacaaaagtg tctataatca
3181 cggcagaaaa gtccacattg attatttgca cggcgtcaca ctttgctatg ccatagcatt
3241 tttatccata agattagcgg atcctacctg acgcttttta tcgcaactct ctactgtttc
3301 tccatacccg tttttttggg ctagcgaatt cgagctcggt acccaagtct taaactagac
3361 agaatagttg taaactgaaa tcagtccagt tatgctgtga aaaagcatac tggacttttg
3421 ttatggctaa agcaaactct tcattttctg aagtgcaaat tgcccgtcgt attaaagagg
3481 ggcgtggcca agggcatggt aaagactata ttccatggct aacagtacaa gaagttcctt
3541 cttcaggtcg ttcccaccgt atttattctc ataagacggg acgagtccat catttgctat
3601 ctgacttaga gcttgctgtt tttctcagtc ttgagtggga gagcagcgtg ctagatatac
3661 gcgagcagtt ccccttatta cctagtgata ccaggcagat tgcaatagat agtggtatta
3721 agcatcctgt tattcgtggt gtagatcagg ttatgtctac tgatttttta gtggactgca
3781 aagatggtcc ttttgagcag tttgctattc aagtcaaacc tgcagcagcc ttacaagacg
3841 agcgtacctt agaaaaacta gaactagagc gtcgctattg gcagcaaaag caaattcctt
3901 ggttcatttt tactgataaa gaaataaatc ccgtagtaaa agaaaatatt gaatggcttt
3961 attcagtgaa aacagaagaa gtttctgcgg agcttttagc acaactatcc ccattggccc
4021 atatcctgca agaaaaagga gatgaaaaca ttatcaatgt ctgtaagcag gttgatattg
4081 cttatgattt ggagttaggc aaaacattga gtgagatacg agccttaacc gcaaatggtt
4141 ttattaagtt caatatttat aagtctttca gggcaaataa gtgtgcagat ctctgtatta
4201 gccaagtagt gaatatggag gagttgcgct atgtggcaaa ttaatgaggt tgtgctattt
4261 gataatgatc cgtatcgcat tttggctata gaggatggcc aagttgtctg gatgcaaata
4321 agcgctgata aaggagttcc acaagctagg gctgagttgt tgctaatgca gtatttagat
4381 gaaggccgct tagttagaac tgatgaccct tatgtacatc ttgatttaga agagccgtct
4441 gtagattctg tcagcttcca gaagcgcgag gaggattatc gaaaaattct tcctattatt
4501 aatagtaagg atcgtttcga ccctaaagtc agaagcgaac tcgttgagca tgtggtccaa
4561 gaacataagg ttactaaggc tacagtttat aagttgttac gccgttactg gcagcgtggt
4621 caaacgccta atgcattaat tcctgactac aaaaacagcg gtgcaccagg ggaaagacgt
4681 tcagcgacag gaacagcaaa gattggccga gccagagaat atggtaaggg tgaaggaacc
4741 aaggtaacgc ccgagattga acgccttttt aggttgacca tagaaaagca cctgttaaat
4801 caaaaaggta caaagaccac cgttgcctat agacgatttg tggacttgtt tgctcagtat
4861 tttcctcgca ttccccaaga ggattaccca acactacgtc agtttcgtta tttttatgat
4921 cgagaatacc ctaaagctca gcgcttaaag tctagagtta aagcaggggt atataaaaaa
4981 gacgtacgac ccttaagtag tacagccact tctcaggcgt taggccctgg gagtcgttat
5041 gagattgatg ccacgattgc tgatatttat ttagtggatc atcatgatcg ccaaaaaatc
5101 ataggaagac caacgcttta cattgtgatt gatgtgttta gtcggatgat cacgggcttt
5161 tatatcggct ttgaaaatcc gtcttatgtg gtggcgatgc aggcttttgt aaatgcttgc
5221 tctgacaaaa cggccatttg tgcccagcat gatattgaga ttagtagctc agactggccg
5281 tgtgtaggtt tgccagatgt gttgctagcg gaccgtggcg aattaatgag tcatcaggtc
5341 gaagccttag tttctagttt taatgtgcga gtggaaagtg ctccacctag acgtggcgat
5401 gctaaaggca tagtggaaag cacttttaga acactacaag ccgagtttaa gtcctttgca
5461 cctggcattg tagagggcag tcggatcaaa agccatggtg aaacagacta taggttagat
5521 gcatctctgt cggtatttga gttcacacaa attattttgc gtacgatctt attcagaaat
5581 aaccatctgg tgatggataa atacgatcga gatgctgatt ttcctacaga tttaccgtct
5641 attcctgtcc agctatggca atggggtatg cagcatcgta caggtagttt aagggctgtg
5701 gagcaagagc agttgcgagt agcgttactg cctcgccgaa aggtctctat ttcttcattt
5761 ggcgttaatt tgtggggttt gtattactcg gggtcagaga ttctgcgtga gggttggttg
5821 cagcggagca ctgatatagc tagacctcaa catttagaag cggcttatga cccagtgctg
5881 gttgatacga tttatttgtt tccgcaagtt ggcagccgtg tattttggcg ctgtaatctg
5941 acggaacgta gtcggcagtt taaaggtctc tcattttggg aggtttggga tatacaagca
6001 caagaaaaac acaataaagc caatgcgaag caggatgagt taactaaacg cagggagctt
6061 gaggcgttta ttcagcaaac cattcagaaa gcgaataagt taacgcccag tactactgag
6121 cccaaatcaa cacgcattaa gcagattaaa actaataaaa aagaagccgt gacctcggag
6181 cgtaaaaaac gtgcggagca tttgaagcca agctcttcag gtgatgaggc taaagttatt
6241 cctttcaacg cagtggaagc ggatgatcaa gaagattaca gcctacccac atacgtgcct
6301 gaattatttc aggatccacc agaaaaggat gagtcatgag tgctacccgg attcaagcag
6361 tttatcgtga tacgggggta gaggcttatc gtgataatcc ttttatcgag gccttaccac
6421 cattacaaga gtcagtgaat agtgctgcat cactgaaatc ctctttacag cttacttcct
6481 ctgacttgca aaagtcccgt gttatcagag ctcataccat ttgtcgtatt ccagatgact
6541 attttcagcc attaggtacg catttgctac taagtgagcg tatttcggtc atgattcgag
6601 gtggctacgt aggcagaaat cctaaaacag gagatttaca aaagcattta caaaatggtt
6661 atgagcgtgt tcaaacggga gagttggaga catttcgctt tgaggaggca cgatctacgg
6721 cacaaagctt attgttaatt ggttgttctg gtagtgggaa gacgacctct cttcatcgta
6781 ttctagccac gtatcctcag gtgatttacc atcgtgaact caatgtagag caggtggtgt
6841 atttgaaaat agactgctcg cataatggtt cgctaaaaga aatctgcttg aattttttca
6901 gagcgttgga tcgagccttg ggctcgaact atgagcgtcg ttatggctta aaacgtcatg
6961 gtatagaaac catgttggct ttgatgtcgc aaatagccaa tgcacatgct ttagggttgt
7021 tggttattga tgaaattcag catttaagcc gctctcgttc gggtggatct caagagatgc
7081 tgaacttttt tgtgacgatg gtgaatatta ttggcgtacc agtgatgttg attggtaccc
7141 ctaaagcacg agagattttt gaggctgatt tgcggtctgc acgtagaggg gcagggtttg
7201 gagctatatt ctgggatcct atacaacaaa cgcaacgtgg aaagcccaat caagagtgga
7261 tcgcttttac ggataatctt tggcaattac agcttttaca acgcaaagat gcgctgttat
7321 cggatgaggt ccgtgatgtg tggtatgagc taagccaagg agtgatggac attgtagtaa
7381 aactttttgt actcgctcag ctccgtgcgc tagctttagg caatgagcgt attaccgctg
7441 gtttattgcg gcaagtgtat caagatgagt taaagcctgt gcaccccatg ctagaggcat
7501 tacgctcggg tatcccagaa cgcattgctc gttattctga tctagtcgtt cccgagattg
7561 ataaacggtt aatccaactt cagctagata tcgcagcgat acaagaacaa acaccagaag
7621 aaaaagccct tcaagagtta gataccgaag atcagcgtca tttatatctg atgctgaaag
7681 aggattacga ttcaagcctg ttaattccca ctattaaaaa agcgtttagc cagaatccaa
7741 cgatgacaag acaaaagtta ctgcctcttg ttttgcagtg gttgatggaa ggcgaaacgg
7801 tagtgtcaga actagaaaag ccctccaaga gtaaaaaggt ttcggctata aaggtagtca
7861 agcccagcga ctgggatagc ttgcctgata cggatttacg ttatatctat tcacaacgcc
7921 aacctgaaaa aaccatgcat gaacggttaa aagggaaagg ggtaatagtg gatatggcga
7981 gcttatttaa acaagcaggt tagccatgag aaactttcct gttccgtact cgaatgagct
8041 gatttatagc actattgcac gggcaggcgt ttatcaaggg attgttagtc ctaagcagct
8101 gttggatgag gtgtatggca accgcaaggt ggtcgctacc ttaggtctgc cctcgcattt
8161 aggtgtgata gcaagacatc tacatcaaac aggacgttac gctgttcagc agcttattta
8221 tgagcatacc ttattccctt tatatgctcc gtttgtaggc aaggagcgcc gagacgaagc
8281 tattcggtta atggagtacc aagcgcaagg tgcggtgcat ttaatgctag gagtcgctgc
8341 ttctagagtt aagagcgata accgctttag atactgccct gattgcgttg ctcttcagct
8401 aaataggtat ggggaagcct tttggcaacg agattggtat ttgcccgctt tgccatattg
8461 tccaaaacac ggtgctttag tcttctttga tagagctgta gatgatcacc gacatcaatt
8521 ttgggctttg ggtcatactg agctgctttc agactacccc aaagactccc tatctcaatt
8581 aacagcacta gctgcttata tagcccctct gttagatgct ccacgagcgc aagagctttc
8641 cccaagcctt gagcagtgga cgctgtttta tcagcgctta gcgcaggatc tagggctaac
8701 caaaagcaag cacattcgtc atgacttggt ggcggagaga gtgaggcaga cttttagtga
8761 tgaggcacta gagaaactgg atttaaagtt ggcagagaac aaggacacgt gttggctgaa
8821 aagtatattc cgtaagcata gaaaagcctt tagttattta cagcatagta ttgtgtggca
8881 agccttattg ccaaaactaa cggttataga agcgctacag caggcaagtg ctcttactga
8941 gcactctata acgacaagac ctgttagcca gtctgtgcaa cctaactctg aagatttatc
9001 tgttaagcat aaagactggc agcaactagt gcataaatac caaggaatta aggcggcaag
9061 acagtcttta gagggtgggg tgctatacgc ttggctttac cgacatgaca gggattggct
9121 agttcactgg aatcaacagc atcaacaaga gcgtctggca cccgccccta gagttgattg
9181 gaaccaaaga gatcgaattg ctgtacgaca actattaaga atcataaagc gtctagatag
9241 tagccttgat cacccaagag cgacatcgag ctggctgtta aagcaaactc ctaacggaac
9301 ctctcttgca aaaaatctac agaaactgcc tttggtagcg ctttgcttaa agcgttactc
9361 agagagtgtg gaagattatc aaattagacg gattagccaa gcttttatta agcttaaaca
9421 ggaagatgtt gagcttaggc gctggcgatt attaagaagt gcaacgttat ctaaagagcg
9481 gataactgag gaagcacaaa gattcttgga aatggtttat ggggaagagt gagtggttag
9541 gctagctaca tttaatgaca atgtgcaggt tgtacatatt ggtcatttat tccgtaactc
9601 gggtcataag gagtggcgta tttttgtttg gtttaatcca atgcaagaac ggaaatggac
9661 tcgatttact catttgcctt tattaagtcg agctaaggtg gttaacagta caacaaagca
9721 aataaataag gcggatcgtg tgattgagtt tgaagcatcg gatcttcaac gagccaaaat
9781 aatcgatttt cctaatctct cgtcctttgc ttccgtacgc aacaaggatg gagcgcagag
9841 ttcatttatt tacgaagctg aaacaccata tagcaagact cgttatcaca tcccacagtt
9901 agagctagct cggtcattat ttttagggga tcctctagag tcgacctgca ggcatgcaag
9961 cttggctgtt ttggcggatg agagaagatt ttcagcctga tacagattaa atcagaacgc
10021 agaagcggtc tgataaaaca gaatttgcct ggcggcagta gcgcggtggt cccacctgac
10081 cccatgccga actcagaagt gaaacgccgt agcgccgatg gtagtgtggg gtctccccat
10141 gcgagagtag ggaactgcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
10201 ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccggg
10261 agcggatttg aacgttgcga agcaacggcc cggagggtgg cgggcaggac gcccgccata
10321 aactgccagg catcaaatta agcagaaggc catcctgacg gatggccttt ttgcgtttct
10381 acaaactctt ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
10441 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
10501 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
10561 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
10621 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
10681 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag
10741 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
10801 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
10861 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
10921 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
10981 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa
11041 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
11101 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
11161 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
11221 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
11281 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
11341 aactgtcaga ccaagtttac tcatatatac tttagattga tttacgcgcc ctgtagcggc
11401 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
11461 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cgccggccag
11521 cctcgcagag caggattccc gttgagcacc gccaggtgcg aataagggac agtgaagaag
11581 gaacacccgc tcgcgggtgg gcctacttca cctatcctgc ccggcggcat caccggcgcc
11641 acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg atggggaaga tcgggctcgc
11701 cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg
11761 ggactgttgg gcgccatctc cttgcatgca ccattccttg cggcggcggt gctcaacggc
11821 ctcaacctac tactgggctg cttcctaatg caggagtcgc ataagggaga gcgtcgatcc
11881 ccgacagtaa gacgggtaag cctgttgatg ataccgctgc cttactgggt gcattagcca
11941 gtctgaatga cctgtcacgg gataatccga agtggtcaga ctggaaaatc agagggcagg
12001 aactgctgaa cagcaaaaag tcagatagca ccacatagca gacccgccat aaaacgccct
12061 gagaagcccg tgacgggctt ttcttgtatt atgggtagtt tccttgcatg aatccataaa
12121 aggcgcctgt agtgccattt acccccattc actgccagag ccgtgagcgc agcgaactga
12181 atgtcacgaa aaagacagcg actcaggtgc ctgatggtcg gagacaaaag gaatattcag
12241 cgatttgccc gagcttgcga gggtgctact taagccttta gggttttaag gtctgttttg
12301 tagaggagca aacagcgttt gcgacatcct tttgtaatac tgcggaactg actaaagtag
12361 tgagttatac acagggctgg gatctattct ttttatcttt ttttattctt tctttattct
12421 ataaattata accacttgaa tataaacaaa aaaaacacac aaaggtctag cggaatttac
12481 agagggtcta gcagaattta caagttttcc agcaaaggtc tagcagaatt tacagatacc
12541 cacaactcaa aggaaaagga ctagtaatta tcattgacta gcccatctca attggtatag
12601 tgattaaaat cacctagacc aattgagatg tatgtctgaa ttagttgttt tcaaagcaaa
12661 tgaactagcg attagtcgct atgacttaac ggagcatgaa accaagctaa ttttatgctg
12721 tgtggcacta ctcaacccca cgattgaaaa ccctacaagg aaagaacgga cggtatcgtt
12781 cacttataac caatacgttc agatgatgaa catcagtagg gaaaatgctt atggtgtatt
12841 agctaaagca accagagagc tgatgacgag aactgtggaa atcaggaatc ctttggttaa
12901 aggctttgag attttccagt ggacaaacta tgccaagttc tcaagcgaaa aattagaatt
12961 agtttttagt gaagagatat tgccttatct tttccagtta aaaaaattca taaaatataa
13021 tctggaacat gttaagtctt ttgaaaacaa atactctatg aggatttatg agtggttatt
13081 aaaagaacta acacaaaaga aaactcacaa ggcaaatata gagattagcc ttgatgaatt
13141 taagttcatg ttaatgcttg aaaataacta ccatgagttt aaaaggctta accaatgggt
13201 tttgaaacca ataagtaaag atttaaacac ttacagcaat atgaaattgg tggttgataa
13261 gcgaggccgc ccgactgata cgttgatttt ccaagttgaa ctagatagac aaatggatct
13321 cgtaaccgaa cttgagaaca accagataaa aatgaatggt gacaaaatac caacaaccat
13381 tacatcagat tcctacctac ataacggact aagaaaaaca ctacacgatg ctttaactgc
13441 aaaaattcag ctcaccagtt ttgaggcaaa atttttgagt gacatgcaaa gtaagtatga
13501 tctcaatggt tcgttctcat ggctcacgca aaaacaacga accacactag agaacatact
13561 ggctaaatac ggaaggatct gaggttctta tggctcttgt atctatcagt gaagcatcaa
13621 gactaacaaa caaaagtaga acaactgttc accgttacat atcaaaggga aaactgtcca
13681 tatgcacaga tgaaaacggt gtaaaaaaga tagatacatc agagctttta cgagtttttg
13741 gtgcatttaa agctgttcac catgaacaga tcgacaatgt aacagatgaa cagcatgtaa
13801 cacctaatag aacaggtgaa accagtaaaa caaagcaact agaacatgaa attgaacacc
13861 tgagacaact tgttacagct caacagtcac acatagacag cctgaaacag gcgatgctgc
13921 ttatcgaatc aaagctgccg acaacacggg agccagtgac gcctcccgtg gggaaaaaat
13981 catggcaatt ctggaagaaa tagcgctttc agcctgtggg cggacaaaat agttgggaac
14041 tgggaggggt ggaaatggag tttttaagga ttatttaggg aagagtgaca aaatagatgg
14101 gaactgggtg tagcgtcgta agctaatacg aaaattaaaa atgacaaaat agtttggaac
14161 tagatttcac ttatctggtt ggtcgacact agtattaccc tgttatccct agatttaaat
14221 gatatcggat cctagtaagc cacgttttaa ttaatcagat ccc
//
