| 质粒名称: | pAAVLINK-EF1alpha-HA-5'-RFX7 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001889 |
| 产品用途: | 5' AAVLINK plasmid for RFX7 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | EF1alpha |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | RFX7 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EF1alpha-HA-5'-RFX7
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EF1alpha-HA-5'-RFX7载体质粒基本信息
pAAVLINK-EF1alpha-HA-5'-RFX7质粒图谱载体图谱和pAAVLINK-EF1alpha-HA-5'-RFX7载体序列质粒序列多克隆位点信息
pAAVLINK-EF1alpha-HA-5'-RFX7质粒载体简介
pAAVLINK-EF1alpha-HA-5'-RFX7质粒序列载体序列
LOCUS pAAVLINK-EF1alpha-HA-5'-RFX7 5739 bp DNA circular SYN 25-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5739)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 12, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5739
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
/note="color: #ffe4c4"
promoter 158..700
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 187..398
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 411..679
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 505..510
/label=SDS
CDS 716..742
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 749..2879
/codon_start=1
/gene="RFX7"
/gene_synonym="MRD71; RFXDC2"
/product="DNA-binding protein RFX7 isoform 1"
/label=RFX7
/note="isoform 1 is encoded by transcript variant
1;regulatory factor X domain containing 2; DNA-binding
protein RFX7; regulatory factor X domain-containing protein
2; regulatory factor X, 7"
/db_xref="CCDS:CCDS92005.1"
/db_xref="GeneID:64864"
/db_xref="HGNC:HGNC:25777"
/db_xref="MIM:612660"
/protein_id="NP_073752.6"
/translation="MAEEQQQPPPQQPDAHQQLPPSAPNSGVALPALVPGLPGTEASAL
QHKIKNSICKTVQSKVDCILQEVEKFTDLEKLYLYLQLPSGLSNGEKSDQNAMSSSRAQ
QMHAFSWIRNTLEEHPETSLPKQEVYDEYKSYCDNLGYHPLSAADFGKIMKNVFPNMKA
RRLGTRGKSKYCYSGLRKKAFVHMPTLPNLDFHKTGDGLEGAEPSGQLQNIDEEVISSA
CRLVCEWAQKVLSQPFDTVLELARFLVKSHYIGTKSMAALTVMAAAPAGMKGITQPSAF
IPTAESNSFQPQVKTLPSPIDAKQQLQRKIQKKQQEQKLQSPLPGESAAKKSESATSNG
VTNLPNGNPSILSPQPIGIVVAAVPSPIPVQRTRQLVTSPSPMSSSDGKVLPLNVQVVT
QHMQSVKQAPKTPQNVPASPGGDRSARHRYPQILPKPANTSALTIRSPTTVLFTSSPIK
TAVVPASHMSSLNVVKMTTISLTPSNSNTPLKHSASVSSATGTTEESRSVPQIKNGSVV
SLQSPGSRSSSAGGTSAVEVKVEPETSSDEHPVQCQENSDEAKAPQTPSALLGQKSNTD
GALQKPSNEGVIEIKATKVCDQRTKCKSRCNEMLPGTSTGNNQSTITLSVASQNLTFTS
SSSPPNGDSINKDPKLCTKSPRKRLSSTLQETQVPPVKKPIVEQLSAATIEGQKQGSVK
KDQKVPHSGKTEGSTA"
misc_feature 2756..2761
/label=SDS
intron 2880..2920
/label=Splicing Donor
misc_feature 2924..2957
/label=lox2272-JT1571
repeat_region 3002..3142
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3002..3131
/label=AAV2 ITR
repeat_region 3003..3143
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
rep_origin 3217..3672
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3954..4058
/gene="bla"
/label=AmpR promoter
CDS 4059..4919
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5090..5678
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgtaccc
721 ttatgacgta cctgactatg ctggatctat ggcagaggaa caacaacagc cgccaccaca
781 gcagcctgat gcccatcagc agcttccccc cagcgccccc aactcggggg tggccctgcc
841 agcccttgtg cccgggctgc cagggacaga ggccagcgcg ctgcaacaca agatcaagaa
901 ctccatctgc aaaactgtac aatctaaagt ggactgcatt ttgcaagaag ttgagaagtt
961 tacagaccta gagaaactct acctctacct tcagctgcct tctggtctca gcaatggaga
1021 gaaaagtgat cagaatgcca tgtcatctag tcgggcacaa caaatgcatg ccttttcctg
1081 gattcggaat accctagagg aacatccgga gacttcactg cccaaacagg aagtctatga
1141 tgagtacaag agctattgtg acaatcttgg ttaccatcca ttaagtgctg ctgattttgg
1201 aaagatcatg aaaaacgtct ttccaaacat gaaggcacgt cgtttgggca caagaggcaa
1261 atctaaatat tgctacagtg gactaagaaa aaaagctttt gttcatatgc caacactgcc
1321 caaccttgac tttcacaaaa ctggagatgg gttggaagga gctgaacctt ctgggcagct
1381 tcaaaatatt gatgaagaag ttatctcttc tgcttgccgt cttgtgtgtg agtgggccca
1441 gaaagtgtta agccaaccat ttgacaccgt cttggaatta gcccgcttcc ttgtaaaaag
1501 tcactatata ggcaccaagt caatggcagc tctaactgta atggcagcag caccagcagg
1561 aatgaaagga attacccagc cttctgcttt tatacctaca gctgaaagta attcctttca
1621 gcctcaggtg aagactttgc catctccaat tgatgctaaa cagcagttgc aacggaaaat
1681 ccagaagaag cagcaagaac agaaactaca atcccctttg ccaggagaat ctgcagcaaa
1741 aaagtcagaa agtgctacaa gcaatggagt gactaatctt cctaatggaa atccttcaat
1801 cctttctcct caacctattg gtatcgttgt ggcagctgtc cctagtccca ttccggtcca
1861 gcggactagg caattggtaa cttcaccgag tccaatgagt tcttctgacg gcaaagttct
1921 tcccctcaat gtacaggtgg tcactcagca catgcagtct gtgaaacagg caccaaagac
1981 tccccagaac gttccagcca gtcctggtgg ggatcgttct gcccggcacc gttaccctca
2041 gatcttaccc aaaccagcga acaccagtgc actcaccatt cgctctccaa ctactgtcct
2101 ctttactagt agtcccatca aaactgctgt tgtacccgct tcacacatga gttctctaaa
2161 tgtggtgaaa atgacaacaa tatccctcac acccagcaac agtaacaccc ctcttaaaca
2221 ttctgcctca gtcagcagtg ctacaggaac aacagaagaa tcaaggagtg ttccacagat
2281 caagaatggt tctgtcgtgt cgcttcagtc tcctgggtcc aggagcagca gtgcgggggg
2341 aacatctgct gtggaagtca aagtggaacc cgaaacatca tcagatgagc atcctgtaca
2401 gtgccaagag aactctgatg aggctaaagc tccccagaca cctagtgccc ttttggggca
2461 gaaaagtaat acagacggag cactgcagaa accttcaaat gaaggtgtca ttgaaataaa
2521 agcaactaag gtctgtgacc agaggaccaa atgtaaaagt cgctgtaatg aaatgctgcc
2581 aggcacgtca acaggcaata atcaaagcac tatcactcta tcagttgctt ctcagaactt
2641 aactttcacc agcagcagct caccacctaa tggtgactca atcaataaag accctaaatt
2701 atgcactaaa agcccaagaa aacgactgtc ttctacattg caagagaccc aggtgcctcc
2761 tgtaaagaaa ccaattgtgg aacagctttc agcagctacc atagaagggc agaaacaagg
2821 cagtgttaag aaggaccaaa aggttccaca ttcagggaaa acagaaggtt caacagcagg
2881 taagtatcaa ggttacaaga caggtttaag gagaccaata agctattatt cgtataggat
2941 actttatacg aagttattaa gttatggaga ccggtgtggc acgtgcggac cgagcggccg
3001 caggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag
3061 gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag
3121 cgagcgcgca gctgcctgca ggggcgcctg atgcggtatt ttctccttac gcatctgtgc
3181 ggtatttcac accgcatacg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa
3241 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccttagcgc
3301 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
3361 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
3421 aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
3481 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
3541 cactcaactc tatctcgggc tattcttttg atttataagg gattttgccg atttcggtct
3601 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
3661 cgtttacaat tttatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
3721 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
3781 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
3841 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
3901 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
3961 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
4021 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
4081 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
4141 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
4201 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
4261 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
4321 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
4381 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
4441 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
4501 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
4561 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
4621 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
4681 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
4741 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
4801 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
4861 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
4921 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
4981 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
5041 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
5101 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
5161 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
5221 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
5281 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
5341 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
5401 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
5461 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
5521 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
5581 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
5641 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
5701 tacggttcct ggccttttgc tggccttttg ctcacatgt
//
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pAAVLINK-EF1alpha-HA-5'-RFX7质粒载体应用举例
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