| 质粒名称: | pAAVLINK-FLNC-B |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001772 |
| 产品用途: | Body' AAVLINK plasmid for FLNC expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | Promoterless |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | FLNC |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-FLNC-B
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-FLNC-B载体质粒基本信息
pAAVLINK-FLNC-B质粒图谱载体图谱和pAAVLINK-FLNC-B载体序列质粒序列多克隆位点信息
pAAVLINK-FLNC-B质粒载体简介
pAAVLINK-FLNC-B质粒序列载体序列
LOCUS pAAVLINK-FLNC-B 6141 bp DNA circular SYN 22-JAN-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6141)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 8, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6141
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..141
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..141
/label=AAV2 ITR
protein_bind 156..189
/label=loxJTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 190..281
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 282..3297
/codon_start=1
/label=FLNC
/translation="VCAYGPGLKGGLVGTPAPFSIDTKGAGTGGLGLTVEGPCEAKIEC
QDNGDGSCAVSYLPTEPGEYTINILFAEAHIPGSPFKATIRPVFDPSKVRASGPGLERG
KVGEAATFTVDCSEAGEAELTIEILSDAGVKAEVLIHNNADGTYHITYSPAFPGTYTIT
IKYGGHPVPKFPTRVHVQPAVDTSGVKVSGPGVEPHGVLREVTTEFTVDARSLTATGDN
HVTARVLNPSGAKTDTYVTDNGDGTYRVQYTAYEEGVHLVEVLYDEVAVPKSPFRVGVT
EGCDPTRVRAFGPGLEGGLVNKANRFTVETRGAGTGGLGLAIEGPSEAKMSCKDNKDGS
CTVEYIPFTPGDYDVNITFGGRPIPGSPFRVPVKDVVDPGKVKCSGPGLGAGVRARVPQ
TFTVDCSQAGRAPLQVAVLGPTGVAEPVEVRDNGDGTHTVHYTPATDGPYTVAVKYADQ
EVPRSPFKIKVLPAHDASKVRASGPGLNASGIPASLPVEFTIDARDAGEGLLTVQILDP
EGKPKKANIRDNGDGTYTVSYLPDMSGRYTITIKYGGDEIPYSPFRIHALPTGDASKCL
VTVSIGGHGLGACLGPRIQIGQETVITVDAKAAGEGKVTCTVSTPDGAELDVDVVENHD
GTFDIYYTAPEPGKYVITIRFGGEHIPNSPFHVLACDPLPHEEEPSEVPQLRQPYAPPR
PGARPTHWATEEPVVPVEPMESMLRPFNLVIPFAVQKGELTGEVRMPSGKTARPNITDN
KDGTITVRYAPTEKGLHQMGIKYDGNHIPGSPLQFYVDAINSRHVSAYGPGLSHGMVNK
PATFTIVTKDAGEGGLSLAVEGPSKAEITCKDNKDGTCTVSYLPTAPGDYSIIVRFDDK
HIPGSPFTAKITGDDSMRTSQLNVGTSTDVSLKITESDLSQLTASIRAPSGNEEPCLLK
RLPNRHIGISFTPKEVGEHVVSVRKSGKHVTNSPFKILVGPSEIGDASKVRVWGKGLSE
GHTFQVAEFIVDTRNA"
misc_feature 1048..1053
/label=SDS
misc_feature 1654..1659
/label=SDS
misc_feature 2311..2316
/label=SDS
misc_feature 3112..3117
/label=SDS
intron 3298..3338
/label=Splicing Donor
misc_feature 3342..3375
/label=lox2272-JT1571
repeat_region 3404..3544
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3404..3533
/label=AAV2 ITR
rep_origin 3619..4074
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4356..4460
/gene="bla"
/label=AmpR promoter
CDS 4461..5321
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5492..6080
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
121 actccatcac taggggttcc tgcggccgca cgcgtataac ttcgtatagc atacattata
181 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
241 tattggtctt actgacatcc actttgcctt tctctccaca ggtctgtgct tatggcccgg
301 gtctcaaggg tggactggta ggcacccccg cgccattctc catcgacacc aagggggctg
361 gcacaggtgg cctggggctg accgtagagg gcccctgcga ggccaagatc gagtgccagg
421 acaatggtga tggctcatgt gctgtcagct acctgcccac ggagcctggc gagtacacca
481 tcaacatcct gtttgctgag gcccacatcc ctggctcgcc cttcaaagcc accattcggc
541 ctgtgtttga cccgagcaag gtgcgggcca gtggaccggg cctggagcgc ggcaaggtcg
601 gtgaggcagc caccttcact gtggactgct cagaggcagg cgaggcggag ctgaccattg
661 agatcctgtc ggatgccggg gtcaaggccg aggtgctgat ccacaacaac gcggatggca
721 cctaccacat cacctacagc cctgccttcc ctggcaccta caccattacc atcaagtatg
781 gcgggcatcc cgtgcccaaa ttccccaccc gtgtccatgt gcagcctgcg gtcgatacca
841 gtggcgtcaa ggtctcaggg cctggtgttg agccacacgg tgtcctgcgg gaggtgacca
901 ctgagttcac tgtggatgca agatccctaa cagccacagg cgacaaccac gtgacggctc
961 gtgtgctcaa cccctcgggg gccaagacag acacctatgt gacagacaat ggggacggca
1021 cctaccgagt gcagtacacc gcctacgagg agggcgtgca tctggtggag gtcctgtatg
1081 atgaggtcgc tgtgcccaag agccccttcc gagtgggcgt gaccgagggc tgtgatccca
1141 cccgcgtccg agccttcggg ccaggcctgg agggtggctt ggtcaacaag gccaaccgat
1201 tcactgtgga gaccagggga gcgggcaccg ggggccttgg cctagccatc gagggtccct
1261 cggaagccaa gatgtcctgc aaggacaaca aggatggtag ctgcaccgtg gagtacatcc
1321 ccttcactcc tggagactat gacgtcaaca tcaccttcgg ggggcggccc atcccaggga
1381 gcccgttccg cgtgccagtg aaggatgtgg tggaccctgg gaaggtgaag tgctcagggc
1441 cagggctggg ggctggtgtc agggcccggg ttcctcagac cttcacagtg gactgcagtc
1501 aagctggccg ggcgcccctg caggtggctg tgctgggccc cacaggtgtg gccgagcctg
1561 tggaggtgcg ggacaatgga gatggcaccc acactgtcca ctacacccca gccactgacg
1621 ggccctacac ggtagccgtc aagtatgctg accaggaggt gccacgcagc cccttcaaga
1681 tcaaggtcct cccagctcat gatgccagca aggtgcgggc cagcggccca ggcctcaacg
1741 cctctggcat ccctgccagc ctgcctgtgg agttcaccat cgacgcacgg gacgcgggcg
1801 aggggttgct cactgtccag atcttggacc ccgagggtaa gcccaagaag gccaacatcc
1861 gggacaatgg ggatggcacg tacactgtgt cctacctgcc ggacatgagt ggccggtaca
1921 ccatcaccat caagtatggc ggtgatgaga tcccctactc gcccttccgc atccatgctc
1981 tgcccactgg ggatgccagc aagtgcctcg tcacagtgtc cattggaggc catggcctgg
2041 gtgcctgcct gggccctcga atccagattg ggcaggagac ggtgatcacg gtggatgcca
2101 aggcagccgg tgaggggaag gtgacatgca cggtgtccac gccggatggg gcagagctcg
2161 atgtggatgt ggttgagaac catgacggta cctttgacat ctactacaca gcgcccgagc
2221 cgggcaagta cgtcatcacc atccgcttcg ggggtgagca catccccaac agccccttcc
2281 acgtgctggc gtgtgacccc ctgccgcacg aggaggagcc ctctgaagtg ccacagctgc
2341 gccagcccta cgctcctccc cggcccggcg cccgccccac acactgggcc acagaggagc
2401 cagtggtgcc tgtggagcca atggagtcca tgctgaggcc cttcaacctg gtcatcccct
2461 tcgcggtgca gaaaggggag ctcacaggag aggtgcggat gccctcgggg aagacggcac
2521 ggcccaacat caccgacaac aaggacggca ccatcacggt gaggtatgca cccactgaga
2581 aaggcctgca ccagatgggg atcaagtatg acggcaacca catccctggg agccccttac
2641 agttctatgt ggatgccatc aacagccgcc atgtcagtgc ctatgggcca ggcctgagcc
2701 atggcatggt caacaagcca gccaccttca ctattgtcac caaagatgct ggagaagggg
2761 gtctgtcact ggccgtggag ggcccatcca aggcagagat cacctgtaag gacaacaagg
2821 atggcacctg caccgtgtcc tatctgccga ctgcgcctgg agactacagc atcatcgtgc
2881 gcttcgatga caagcacatc ccggggagcc ccttcacagc caagatcaca ggtgatgact
2941 ccatgaggac ctcacagctg aatgtgggca cctccacgga cgtgtcactg aagatcaccg
3001 agagtgatct gagccagctg accgccagca tccgtgcccc ctcgggcaac gaggagccct
3061 gcctgctgaa gcgcctgccc aaccggcaca ttgggatctc cttcaccccc aaggaggtcg
3121 gggagcacgt ggtgagcgtg cgcaagagtg gcaagcatgt caccaacagc cccttcaaga
3181 tcctggtggg gccatctgag atcggggacg ccagcaaggt gcgggtctgg ggcaaggggc
3241 tttccgaggg acacacattc caggtggcag agttcatcgt ggacactcgc aatgcaggta
3301 agtatcaagg ttacaagaca ggtttaagga gaccaataag ctattattcg tataggatac
3361 tttatacgaa gttatgaatt ccacgtgcgg accgagcggc cgcaggaacc cctagtgatg
3421 gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc
3481 gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg
3541 caggggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
3601 cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
3661 ttacgcgcag cgtgaccgct acacttgcca gcgccttagc gcccgctcct ttcgctttct
3721 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
3781 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg
3841 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
3901 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac tctatctcgg
3961 gctattcttt tgatttataa gggattttgc cgatttcggt ctattggtta aaaaatgagc
4021 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca attttatggt
4081 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
4141 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
4201 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
4261 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
4321 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
4381 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
4441 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
4501 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
4561 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
4621 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
4681 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
4741 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
4801 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
4861 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
4921 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
4981 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
5041 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
5101 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
5161 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
5221 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
5281 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
5341 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
5401 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
5461 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
5521 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
5581 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
5641 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
5701 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
5761 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
5821 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
5881 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
5941 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
6001 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
6061 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
6121 gctggccttt tgctcacatg t
//
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