TERT

LOCUS       TERT                9728 bp ds-DNA     circular SYN 

10-NOV-2022
DEFINITION  For use in RBP tethering screen.
ACCESSION   .
VERSION     .
KEYWORDS    TERT
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9728)
  TITLE     Yeo Lab plasmids
REFERENCE   2  (bases 1 to 9728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 10, 2022 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..9728
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human 

elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of 

human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, 

forward primer"
     primer_bind     1196..1215
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1196..1214
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA 

polymerase"
     protein_bind    1293..1317
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) 

BP reaction"
     CDS             complement(4336..4347)
                     /codon_start=1
                     /product="WELQut protease recognition and 

cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    complement(4685..4709)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) 

BP reaction"
     CDS             4834..4875
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(5330..5347)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, 

reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5336..5560
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation 

signal"
     primer_bind     complement(5693..5712)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    5844..5891
                     /label=FRT
                     /bound_moiety="FLP recombinase from the 

Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in 

the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             5899..6921
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase 

from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     

/translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     

VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     

TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     

VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     

DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     

FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    7051..7172
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7088..7107)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7142..7161
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7221..7237)
                     /label=M13 rev
                     /note="common sequencing primer, one of 

multiple similar 
                     variants"
     primer_bind     complement(7221..7237)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7234..7256)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7245..7261
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac 

operator to 
                     inhibit transcription in E. coli. This 

inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside 

(IPTG)."
     promoter        complement(7269..7299)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7314..7335
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator 

protein"
                     /note="CAP binding activates transcription in 

the presence 
                     of cAMP."
     primer_bind     complement(7452..7469)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7623..8211)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC 

origin of 
                     replication"
     primer_bind     complement(7703..7722)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8382..9242)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, 

carbenicillin, and
                     related antibiotics"
                     

/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     

ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     

PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     

EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     

LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9005..9024
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse 

primer"
     promoter        complement(9243..9347)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9422..9441)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast 

selectable 
                     marker"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga 

gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa 

ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta 

tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca 

ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt 

gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg 

aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt 

tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg 

tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct 

ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt 

ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg 

gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc 

tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg 

gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc 

aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag 

ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag 

gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt 

ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca 

cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa 

gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gaattagctt 

ggtactaata
     1201 cgactcacta tagggagacc caagctggct aggtaagctt ggtaccgagc 

tcggatccac
     1261 tagtccagtg tggtggaatt ctgcagatat caacaagttt gtacaaaaaa 

gcaggctgcc
     1321 accatgccgc gcgctccccg ctgccgagcc gtgcgctccc tgctgcgcag 

ccactaccgc
     1381 gaggtgctgc cgctggccac gttcgtgcgg cgcctggggc cccagggctg 

gcggctggtg
     1441 cagcgcgggg acccggcggc tttccgcgcg ctggtggccc agtgcctggt 

gtgcgtgccc
     1501 tgggacgcac ggccgccccc cgccgccccc tccttccgcc aggtgtcctg 

cctgaaggag
     1561 ctggtggccc gagtgctgca gaggctgtgc gagcgcggcg cgaagaacgt 

gctggccttc
     1621 ggcttcgcgc tgctggacgg ggcccgcggg ggcccccccg aggccttcac 

caccagcgtg
     1681 cgcagctacc tgcccaacac ggtgaccgac gcactgcggg ggagcggggc 

gtgggggctg
     1741 ctgctgcgcc gcgtgggcga cgacgtgctg gttcacctgc tggcacgctg 

cgcgctcttt
     1801 gtgctggtgg ctcccagctg cgcctaccag gtgtgcgggc cgccgctgta 

ccagctcggc
     1861 gctgccactc aggcccggcc cccgccacac gctagtggac cccgaaggcg 

tctgggatgc
     1921 gaacgggcct ggaaccatag cgtcagggag gccggggtcc ccctgggcct 

gccagccccg
     1981 ggtgcgagga ggcgcggggg cagtgccagc cgaagtctgc cgttgcccaa 

gaggcccagg
     2041 cgtggcgctg cccctgagcc ggagcggacg cccgttgggc aggggtcctg 

ggcccacccg
     2101 ggcaggacgc gtggaccgag tgaccgtggt ttctgtgtgg tgtcacctgc 

cagacccgcc
     2161 gaagaagcca cctctttgga gggtgcgctc tctggcacgc gccactccca 

cccatccgtg
     2221 ggccgccagc accacgcggg ccccccatcc acatcgcggc caccacgtcc 

ctgggacacg
     2281 ccttgtcccc cggtgtacgc cgagaccaag cacttcctct actcctcagg 

cgacaaggag
     2341 cagctgcggc cctccttcct actcagctct ctgaggccca gcctgactgg 

cgctcggagg
     2401 ctcgtggaga ccatctttct gggttccagg ccctggatgc cagggactcc 

ccgcaggttg
     2461 ccccgcctgc cccagcgcta ctggcaaatg cggcccctgt ttctggagct 

gcttgggaac
     2521 cacgcgcagt gcccctacgg ggtgctcctc aagacgcact gcccgctgcg 

agctgcggtc
     2581 accccagcag ccggtgtctg tgcccgggag aagccccagg gctctgtggc 

ggcccccgag
     2641 gaggaggaca cagacccccg tcgcctggtg cagctgctcc gccagcacag 

cagcccctgg
     2701 caggtgtacg gcttcgtgcg ggcctgcctg cgccggctgg tgcccccagg 

cctctggggc
     2761 tccaggcaca acgaacgccg cttcctcagg aacaccaaga agttcatctc 

cctggggaag
     2821 catgccaagc tctcgctgca ggagctgacg tggaagatga gcgtgcggga 

ctgcgcttgg
     2881 ctgcgcagga gcccaggggt tggctgtgtt ccggccgcag agcaccgtct 

gcgtgaggag
     2941 atcctggcca agttcctgca ctggctgatg agtgtgtacg tcgtcgagct 

gctcaggtct
     3001 ttcttttatg tcacggagac cacgtttcaa aagaacaggc tctttttcta 

ccggaagagt
     3061 gtctggagca agttgcaaag cattggaatc agacagcact tgaagagggt 

gcagctgcgg
     3121 gagctgtcgg aagcagaggt caggcagcat cgggaagcca ggcccgccct 

gctgacgtcc
     3181 agactccgct tcatccccaa gcctgacggg ctgcggccga ttgtgaacat 

ggactacgtc
     3241 gtgggagcca gaacgttccg cagagaaaag agggccgagc gtctcacctc 

gagggtgaag
     3301 gcactgttca gcgtgctcaa ctacgagcgg gcgcggcgcc ccggcctcct 

gggcgcctct
     3361 gtgctgggcc tggacgatat ccacagggcc tggcgcacct tcgtgctgcg 

tgtgcgggcc
     3421 caggacccgc cgcctgagct gtactttgtc aaggacaggc tcacggaggt 

catcgccagc
     3481 atcatcaaac cccagaacac gtactgcgtg cgtcggtatg ccgtggtcca 

gaaggccgcc
     3541 catgggcacg tccgcaaggc cttcaagagc cacgtctcta ccttgacaga 

cctccagccg
     3601 tacatgcgac agttcgtggc tcacctgcag gagaccagcc cgctgaggga 

tgccgtcgtc
     3661 atcgagcaga gctcctccct gaatgaggcc agcagtggcc tcttcgacgt 

cttcctacgc
     3721 ttcatgtgcc accacgccgt gcgcatcagg ggcaagtcct acgtccagtg 

ccaggggatc
     3781 ccgcagggct ccatcctctc cacgctgctc tgcagcctgt gctacggcga 

catggagaac
     3841 aagctgtttg cggggattcg gcgggacggg ctgctcctgc gtttggtgga 

tgatttcttg
     3901 ttggtgacac ctcacctcac ccacgcgaaa accttcctca ggaccctggt 

ccgaggtgtc
     3961 cctgagtatg gctgcgtggt gaacttgcgg aagacagtgg tgaacttccc 

tgtagaagac
     4021 gaggccctgg gtggcacggc ttttgttcag atgccggccc acggcctatt 

cccctggtgc
     4081 ggcctgctgc tggatacccg gaccctggag gtgcagagcg actactccag 

ctatgcccgg
     4141 acctccatca gagccagtct caccttcaac cgcggcttca aggctgggag 

gaacatgcgt
     4201 cgcaaactct ttggggtctt gcggctgaag tgtcacagcc tgtttctgga 

tttgcaggtg
     4261 aacagcctcc agacggtgtg caccaacatc tacaagatcc tcctgctgca 

ggcgtacagg
     4321 tttcacgcat gtgtgctgca gctcccattt catcagcaag tttggaagaa 

ccccacattt
     4381 ttcctgcgcg tcatctctga cacggcctcc ctctgctact ccatcctgaa 

agccaagaac
     4441 gcagggatgt cgctgggggc caagggcgcc gccggccctc tgccctccga 

ggccgtgcag
     4501 tggctgtgcc accaagcatt cctgctcaag ctgactcgac accgtgtcac 

ctacgtgcca
     4561 ctcctggggt cactcaggac agcccagacg cagctgagtc ggaagctccc 

ggggacgacg
     4621 ctgactgccc tggaggccgc agccaacccg gcactgccct cagacttcaa 

gaccatcctg
     4681 gacgacccag ctttcttgta caaagtggtt gatatccagc acagtggcgg 

ccgctcgagt
     4741 ctagagggcc cgcggttcga aaccagcgga ggcggcggaa gcgggggagg 

aggcagcggc
     4801 ggaggcggca gcgggggcgg cggaagcgct accggtaagc ctatccctaa 

ccctctcctc
     4861 ggtctcgatt ctacgcgtac cggtatggct tctaacttta ctcagttcgt 

tctcgtcgac
     4921 aatggcggaa ctggcgacgt gactgtcgcc ccaagcaact tcgctaacgg 

ggtcgctgaa
     4981 tggatcagct ctaactcgcg atcacaggct tacaaagtaa cctgtagcgt 

tcgtcagagc
     5041 tctgcgcaga atcgcaaata caccatcaaa gtcgaggtgc ctaaagtggc 

aacccagact
     5101 gttggtggtg aagagcttcc tgtagccgga tggagatctt acttaaatat 

ggaactaacc
     5161 attccaattt tcgccacgaa ttccgactgc gagcttattg ttaaggcaat 

gcaaggtctc
     5221 ctaaaagatg gaaacccgat tccctcggcc atcgcggcca actccggcat 

ctacggaggt
     5281 ggaggtggat ctcgtacggg ttagtaatga gtttaaaccc gctgatcagc 

ctcgactgtg
     5341 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt 

gaccctggaa
     5401 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca 

ttgtctgagt
     5461 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga 

ggattgggaa
     5521 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc 

ggaaagaacc
     5581 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag 

cgcggcgggt
     5641 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc 

cgctcctttc
     5701 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc 

tctaaatcgg
     5761 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa 

aaaacttgat
     5821 tagggtgatg gttcacgtac ctagaagttc ctattccgaa gttcctattc 

tctagaaagt
     5881 ataggaactt ccttggccaa aaagcctgaa ctcaccgcga cgtctgtcga 

gaagtttctg
     5941 atcgaaaagt tcgacagcgt ctccgacctg atgcagctct cggagggcga 

agaatctcgt
     6001 gctttcagct tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag 

ctgcgccgat
     6061 ggtttctaca aagatcgtta tgtttatcgg cactttgcat cggccgcgct 

cccgattccg
     6121 gaagtgcttg acattgggga attcagcgag agcctgacct attgcatctc 

ccgccgtgca
     6181 cagggtgtca cgttgcaaga cctgcctgaa accgaactgc ccgctgttct 

gcagccggtc
     6241 gcggaggcca tggatgcgat cgctgcggcc gatcttagcc agacgagcgg 

gttcggccca
     6301 ttcggaccgc aaggaatcgg tcaatacact acatggcgtg atttcatatg 

cgcgattgct
     6361 gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc 

gtccgtcgcg
     6421 caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg 

gcacctcgtg
     6481 cacgcggatt tcggctccaa caatgtcctg acggacaatg gccgcataac 

agcggtcatt
     6541 gactggagcg aggcgatgtt cggggattcc caatacgagg tcgccaacat 

cttcttctgg
     6601 aggccgtggt tggcttgtat ggagcagcag acgcgctact tcgagcggag 

gcatccggag
     6661 cttgcaggat cgccgcggct ccgggcgtat atgctccgca ttggtcttga 

ccaactctat
     6721 cagagcttgg ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg 

atgcgacgca
     6781 atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag 

aagcgcggcc
     6841 gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg 

ccccagcact
     6901 cgtccgaggg caaaggaata gcacgtacta cgagatttcg attccaccgc 

cgccttctat
     6961 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct 

ccagcgcggg
     7021 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta 

taatggttac
     7081 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact 

gcattctagt
     7141 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc 

gacctctagc
     7201 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta 

tccgctcaca
     7261 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc 

ctaatgagtg
     7321 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg 

aaacctgtcg
     7381 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg 

tattgggcgc
     7441 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg 

gcgagcggta
     7501 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa 

cgcaggaaag
     7561 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc 

gttgctggcg
     7621 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc 

aagtcagagg
     7681 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag 

ctccctcgtg
     7741 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct 

cccttcggga
     7801 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta 

ggtcgttcgc
     7861 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc 

cttatccggt
     7921 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc 

agcagccact
     7981 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt 

gaagtggtgg
     8041 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct 

gaagccagtt
     8101 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc 

tggtagcggt
     8161 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca 

agaagatcct
     8221 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta 

agggattttg
     8281 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa 

atgaagtttt
     8341 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg 

cttaatcagt
     8401 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg 

actccccgtc
     8461 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc 

aatgataccg
     8521 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc 

cggaagggcc
     8581 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa 

ttgttgccgg
     8641 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc 

cattgctaca
     8701 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg 

ttcccaacga
     8761 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc 

cttcggtcct
     8821 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat 

ggcagcactg
     8881 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg 

tgagtactca
     8941 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc 

ggcgtcaata
     9001 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg 

aaaacgttct
     9061 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat 

gtaacccact
     9121 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg 

gtgagcaaaa
     9181 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg 

ttgaatactc
     9241 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct 

catgagcgga
     9301 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac 

atttccccga
     9361 aaagtgccac ctgacgtcga cggatcggga gatctcccga tcccctatgg 

tgcactctca
     9421 gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct 

tgtgtgttgg
     9481 aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc 

ttgaccgaca
     9541 attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat 

gtacgggcca
     9601 gatatacgcg ttgacattga ttattgacta ggcttttgca aaaagctttg 

caaagatgga
     9661 taaagtttta aacagagagg aatctttgca gctaatggac cttctaggtc 

ttgaaaggag
     9721 tgggaatt
//